InTechOpen uses cookies to offer you the best online experience. By continuing to use our site, you agree to our Privacy Policy.

Immunology and Microbiology » "Lymphocyte Updates - Cancer, Autoimmunity and Infection", book edited by Gheorghita Isvoranu, ISBN 978-953-51-3344-5, Print ISBN 978-953-51-3343-8, Published: July 12, 2017 under CC BY 3.0 license. © The Author(s).

Chapter 2

Signal Pathway in Precursor B-Cell Lymphoblastic Leukemia/Lymphoma

By Tatsuaki Tsuruyama and Takuya Hiratsuka
DOI: 10.5772/intechopen.68892

Article top


Scheme of B-cell development stage and IL-7R/pre–B-cell receptor (BCR) expression. CLP, common lymphocyte precursor.
Figure 1. Scheme of B-cell development stage and IL-7R/pre–B-cell receptor (BCR) expression. CLP, common lymphocyte precursor.
Surface phenotypic markers and Hardy’s classification. BP-1 and IgM are notable markers. Fr., fraction.
Figure 2. Surface phenotypic markers and Hardy’s classification. BP-1 and IgM are notable markers. Fr., fraction.
Ig recombination and B-cell development stage. VpreB and λ5 are components of surrogate light change in the pre-BCR. Igα and β are adaptor molecules that are identical to CD79a and CD79b. Pre-BCR is tentatively formed in the stage of large pre-B.
Figure 3. Ig recombination and B-cell development stage. VpreB and λ5 are components of surrogate light change in the pre-BCR. Igα and β are adaptor molecules that are identical to CD79a and CD79b. Pre-BCR is tentatively formed in the stage of large pre-B.
Bomb1 locus and microsatellite markers on chromosome 3.
Figure 4. Bomb1 locus and microsatellite markers on chromosome 3.
Microsatellite instability in the genome of lymphoma cells in the lane of healthy lymph node tissue [17]. Dinucleotide CT is deleted in the lymphoma genomes.
Figure 5. Microsatellite instability in the genome of lymphoma cells in the lane of healthy lymph node tissue [17]. Dinucleotide CT is deleted in the lymphoma genomes.
(a) Schematic representation of MLV integration in Zfp521 in SL/Kh mice. By the age of 2–3 months, the MLV host cell has grown in BM, and the endogenous MLV integrates into the genome of the host lymphocytes. The host cells clonally grow with higher expression of the Zfp521 gene. The tandem box in the upper scheme represents long terminal repeat (LTR) of the provirus.
Figure 6. (a) Schematic representation of MLV integration in Zfp521 in SL/Kh mice. By the age of 2–3 months, the MLV host cell has grown in BM, and the endogenous MLV integrates into the genome of the host lymphocytes. The host cells clonally grow with higher expression of the Zfp521 gene. The tandem box in the upper scheme represents long terminal repeat (LTR) of the provirus.
Pre-BCR pathway and Zfp521.
Figure 7. Pre-BCR pathway and Zfp521.
Signaling pathway network in association with IL-7R.
Figure 8. Signaling pathway network in association with IL-7R.

Signal Pathway in Precursor B-Cell Lymphoblastic Leukemia/Lymphoma

Tatsuaki Tsuruyama and Takuya Hiratsuka
Show details


Stat5, c-myc, Hipk2, Fiz1, and ZFP521 to lymphomagenesis precursor B-cell lymphoblastic lymphoma/leukemia have been previously identified as a putative gene involved in the induction of B-cell lymphomagenesis. In this review, we summarize the role of ZFP521 in B-cell lymphomagenesis. Zinc finger protein 521 (Zfp521) is a novel identified gene that is responsible for pre–B-lymphoblastic lymphomagenesis through activation of pre–B-cell receptor (pre-BCR)-signaling by upregulation of adaptor genes and related kinases in the signaling downstream. The pre-BCR-signaling molecules, FLT3, CD43, and IL-7 receptor (IL-7R) were positively regulated by these genes. Stimulation of pre-BCR and/or IL-7R signaling caused aberrant upregulation of other oncogene sets such as cyclin genes, thereby inducing the growth of pre–B cells. IL-7R/Janus kinase (JAK)/STAT signaling cascade is one of the key signaling pathways that are activated in precursor B-cell lymphoblastic lymphoma/leukemia. FLT3, CD43, and pre-BCR cascades crosstalk with JAK/STAT cascade. FLT3 and CD43 cascades have the potential to enhance JAK/STAT cascade effect on pre-B cell growth. On the other hand, pre-BCR and interleukin (IL)-7 receptor exerted competitive effects on pre–B-cell growth; thus, precursor B-cell lymphoblastic lymphomagenesis is a consequence through interaction with these cascades.

Keywords: pre–B-cell receptor, Stat/Jak pathway, Zfp521

1. Introduction

1.1. Summary

B-cell lymphoblastic leukemia/lymphoma (B-LBL) is a neoplasm that exhibits immature phenotype of the B-cell lineage with on-going immunoglobulin rearrangement. Understanding the activation of signal pathways in tumor cells provides significant knowledge on tumorigenesis. Surface markers interleukin-7 receptor (IL-7R), FLT3, CD43, and phenotypic marker pre–B-cell receptor are aberrantly activated in tumor cells. IL-7R is one of the developmental stage markers and is closely associated with immunoglobulin gene rearrangement in mice. In addition, these IL-7R, FLT3, and CD43 signal pathways interact with each other. The signaling molecules, JAK3, Stat5a, Fiz1, and Hipk2, play pivotal roles in these signaling pathways. In this review, we summarize the activation networks of these pathways from the perspective of the activation of adaptor molecules and immunoglobulin rearrangement.

1.2. Introduction

B-LBL is a neoplasm of B-lymphoid precursors and it is essentially identical to acute lymphocytic leukemia as it involves the bone marrow and peripheral blood [1, 2]. These lymphomas and leukemias are composed of medium-sized blast cells with scant cytoplasm, an oval nucleus, transparent nucleus, condensed chromatin, and often multiple nucleoli. The lymphoma tissues exhibit mitotic figures and are phagocytosed by macrophages after apoptosis—this histology is called “Starry sky” and is well known in Burkitt lymphoma. Distinguishing B-precursor types from T-precursor types is impossible because they share similar cytological features. Immuno-phenotypes of pre–B LBL resemble the normal immature B-cell lineages, primarily including pre–B cells, because pre–B LBL consists of ongoing immunoglobulin gene (Ig) rearrangements of heavy chains (Igh) or light chains (Igl). This rearrangement depends on the activity of recombination-activating gene 1 (RAG1) and RAG2 under the high expression of the interleukin 7 receptor (IL-7R) [3]. In addition, pre–B receptors consist of lambda5 and Vpreb component, which are surrogate light-chain components at the time of completion of Igh rearrangement (Figure 1) [4, 5].


Figure 1.

Scheme of B-cell development stage and IL-7R/pre–B-cell receptor (BCR) expression. CLP, common lymphocyte precursor.

2. The characterization of spontaneous pre–B-cell lymphoma in SL/Kh mice

2.1. Experimental mouse model of spontaneous lymphoma

We established an inbred strain of mouse called the spontaneous lymphoma mouse strain (SL/Kh) as a model of murine leukemia virus (MLV) integration-induced B-LBL lymphomagenesis. In the experimental model, transgenic mice carrying chimera genes, such as Emu-myc mice, MT-BCR-ABL mice, [6, 7], and TEL/AML1 mice rapidly develop pre-B LBL [810]. Unlike these models, the SL/Kh mouse develops spontaneously in the absence of artificially introduced gene mutation; however, Zfp521 is the gene that is spontaneously and constitutively mutated by MLV insertion after the birth [11, 12].

These mice share MLV with AKR-strain mice that are susceptible to T lymphoma [13, 14]. SL/Kh mice were found to have multiple copies of the pathogenic endogenous proviral genome that are genetically transmitted through the germ line on chr 7 [12, 15]. A type of MLV expressed from this provirus infects the hematopoietic cells and MLV genome is somatically re-integrated into the host cell genome. Subsequently, B-LBL spontaneously develops with a high frequency of 95% after 6 months of birth. These lymphoma cells are positive for lambda5 and Vpreb, which are a part of the pre-BCR. Myeloid leukemia, mature B-cell lymphoma, and T-cell lymphoma are known to occur in the inbred strain of mouse [16]. Such high occurrence of identical B-lymphoblastic lymphoma/leukemia phenotypes has not been reported in other mice. The initial growth of pre–B cells in SL/Kh was proven to be independent of the provirus integration, but dependent on the bone marrow pre-B1 (Bomb1) locus that includes BANK1 and the enpep gene that involves a glutamyl aminopeptidase (BP-1) (Mm.1193, UniGeneID) [17]. Clinically, the mice present with hepatosplenomegaly in which pre–B LBL invades via the portal tract and replace the splenocytes. In addition, the spinal bone becomes deformed, because of bone structure remodeling. As described later, the identified signal cascade promoting the MLV proviral element gives the clue for understanding of the development of lymphomagenesis through upregulation of signaling pathways and can serve as a model of clinical intervention by administration of anti-tumor drugs because of stable susceptibility for lymphomagenesis.

2.2. Flow cytometry analysis of B-LBL experimental lymphomas

Flow cytometric analysis is the one of the most important methods for analyzing pre–B cells. BP1, B220, IL-7R, CD24, and CD43 are the classical phenotypic markers of pre–B cells as well as λ5 and Vpreb. These markers were available for Hardy’s classification for murine B cell lineage (Figures 2 and 3) [18, 19]. These markers are a little different from those that are used for the classification of human B-cell lineages, because B220 , BP-1, CD43, and CD24 are included.


Figure 2.

Surface phenotypic markers and Hardy’s classification. BP-1 and IgM are notable markers. Fr., fraction.


Figure 3.

Ig recombination and B-cell development stage. VpreB and λ5 are components of surrogate light change in the pre-BCR. Igα and β are adaptor molecules that are identical to CD79a and CD79b. Pre-BCR is tentatively formed in the stage of large pre-B.

2.3. Genetic background of pre–B lymphomagenesis

Bomb1, a quantitative trait locus (QTL), on Mus musculus (MMU) chromosome 3 is responsible for pre–B-cell expansion [20, 21] (Figure 4). In analysis of the congenic mice carrying SL/Kh alleles of Bomb1, polyclonal expansion of pre–B cells is observed. BANK1, an adaptor molecule of pre-BCR, is located near the Bomb1 locus. We generated a congenic strain, NFS.SL/Kh-Bomb1 mice, with the replacement of this locus with SL/Kh Bomb1, without pre–B-induced provirus. The congenic mice showed pre–B-cell expansion, but pre–B lymphomagenesis were not observed. Therefore, the pre–B-cell lymphomagenesis is probably induced by multiple genes, including MLV integration into the proto-oncogenes. Notably, this locus is also susceptible to high-frequency microsatellite instability (MSI) in the pre–B LBL in mouse chromosome 3 including the Bomb1 [17] (Figure 5). MSI is confirmed at ≥2 markers in DNA derived from tumor tissues in 93.7% of SL/Kh mice. To date, there have been only few systematic analyses of MSI and our data are significant in the hematopoietic tumors. Irregular deletion and insertion are observed within Bomb1 in the course of lymphoma tissue with a high frequency.


Figure 4.

Bomb1 locus and microsatellite markers on chromosome 3.


Figure 5.

Microsatellite instability in the genome of lymphoma cells in the lane of healthy lymph node tissue [17]. Dinucleotide CT is deleted in the lymphoma genomes.

3. Upregulation of proto-oncogenes in pre–B lymphomas

Retroviral tagging, such as MLV insertion, is considered as a useful method for the identification of proto-oncogenes. RTCGD (Retrovirus and Transposon-tagged Cancer Gene Database, is one of the established registration systems of MLV integration, and many genes were identified as the common integration site (CIS) [16].

There many identified genes that are involved in the development of hematopoietic tumors. We summarize the signaling pathways that are associated with the target genes as described in the subsequent text.

3.1. IL-7R-signaling pathway and Stat5a

In both humans and mice, the IL-7R (also known as CD127) is expressed by early B-cell progenitors, and signaling via IL-7Rα activates signal transducer and activator of transcription 5 (STAT5) and drives pro-B-cell proliferation, while inhibiting Igκ recombination [22, 23].

Stat5a gene is one of the target genes of MLV integration in B-cell tumors (Table 1). The encoded STAT5 protein is a member of the signal inducer and activator of transcription (STAT) family and includes STAT5A and STAT5B subtypes. They are encoded by separate genes—the proteins are 90% identical at the amino acid level. These encoding genes are both targets of MLV. STAT5 proteins are activated by Janus kinases (JAKs) associated with transmembrane receptors such as interleukin receptor. Because, deletion of Stat5a and Stat5b arrests B-cell development at the pre–pro-B cell stage [24].

GeneMean interval (bp)Number of integration sites
MHC class heavy chain1002

Table 1. 

Common integration site.

Binding of the cytokine ligands to these receptors on the outside of the cell activates the JAK3 [25]. Subsequently, the activated kinases add a phosphate group to tyrosine residues (Y449) on the IL-7Rα chain of the receptor. STAT5 then binds to these phosphorylated tyrosines. STAT5 is subsequently phosphorylated by the JAK3. The phosphorylated STAT5 forms either homodimer. Phosphorylated STAT proteins have the potential to form a dimer that can translocate into the nucleus and upregulate transcriptional activity by binding to the gamma interferon activation site palindromic (GAS) element in the promoters of the target genes. The targets encode c-Myc, Pim-1 [26], Bcl-xL, and Cyclin D1 [27], which promote proliferation and apoptosis in hematopoietic cells [28]. STAT5A, in particular, contributes to IL-7–induced B-cell precursor expansion. IL-7R is highly expressed in pre–B cells during Igh recombination, and therefore Stat5a has been one of the responsible molecules for Igh recombination [29]. Attenuation of IL-7R signaling in both human and mouse pre–B cells is associated with the expression of RAG1 and RAG2.

A comparison of the phenotype of SL/Kh lymphomas showed that when the Stat5a was highly expressed, clones completed Igh DHJHrecombination but not Igh variable segment—DH recombination; on the other hand, when the Stat5a was relatively less expressed in clones, both DHJH and Igh variable segment, DH recombination, are completed. On the other hand, Stat5a-high clones highly express λ5 but low for Vpreb; by contrast, Stat5a-low clones were constitutively high for both λ5 and Vpreb. In summary, the Stat5a-high lymphoma clones are more immature than other lymphomas. Stat5a may contribute to the lymphomagenesis at the immature stage of B cells [29].

3.2. Zfp521 and pre-BCR pathway

The Zfp521 gene was identified at the MLV integration site in the genomes of B-cell lymphomas in the AKXD mouse strain [30, 31]. This gene is also the most frequent integration site as well in the genome of pre–B-cell lymphoma in SL/Kh mice (Figure 6) and is related to immature B lymphomagenesis [11, 32]. Zfp521 expression contributes to neural crest formation and the development of adipose cells, chondrocytes [33, 34], bone [3436], and neural crest [35]. Recently, we reported that ZFP521 regulates and activates pre–B-cell receptor signal pathways, and it modulates the IL-7-signaling pathway [11].


Figure 6.

(a) Schematic representation of MLV integration in Zfp521 in SL/Kh mice. By the age of 2–3 months, the MLV host cell has grown in BM, and the endogenous MLV integrates into the genome of the host lymphocytes. The host cells clonally grow with higher expression of the Zfp521 gene. The tandem box in the upper scheme represents long terminal repeat (LTR) of the provirus.

The pre-BCR is expressed on large pre–B cells in which Igh recombination is completed. In the initiation of Igκ or Igλ gene rearrangement, signals of the IL-7 receptor gradually attenuate in pre–B cells, and B-cell maturation proceeds. Although both the IL-7R and the pre-BCR are required for murine B-cell lymphopoiesis, the orchestration of signal pathways has remained controversial. The responsiveness to IL-7 and stimulation through pre-BCR controls the development of pre–B cells into mature B cells [22, 23]. During the development of pro-B cells into pre–B cells, IL-7 signaling is the major mediator. The mature BCR replaces the pre-BCR. Zfp521 is expressed from professional pre–B cell of Fraction A (Fr. A) to Fr. B-C according to Hardy’s classification. In this pre–B stage, Zfp521 may interact with adaptor molecules of Cd79a/b such as BANK1, Blnk, and Btk. Zfp521 may play as a transcriptional factor, because of a stimulation of this gene expression in a cell line, and the signal was located in the nucleus [30]. However, the binding motif on DNA is not clearly identified. The IL-7 receptor pathway interfered with Vpreb stimulation through the upregulation of BANK1 near or on Bomb1 by ZFP521. BANK1 is disrupted by IL-7R signaling and interacts with phospholipase gamma 2 [37]. In fact, BANK1-PLCg2 binding is enhanced by B-lymphocyte kinase (BLK) [37]. Therefore, complicated per-BCR adaptors are hypothesized (Figure 7).


Figure 7.

Pre-BCR pathway and Zfp521.

Cyclin D3 and Cyclin D2 are upregulated by overexpression of the ZFP521 gene. Pre-BCR was shown to mediate Ras-MEK-extracellular signal-regulated kinase (ERK)-signaling pathway activation and light-chain recombination by silencing Cyclin D3 [38].

In humans, the fusion of the Pax5, which is essential for pre–B-cell development gene, exon 7 to ZFP521 exon 4, has been observed in pre–B-cell acute lymphocytic leukemia by genome-wide analysis of genetic alterations [39]. Dysregulation of ZFP521 gene leads to pre–B-cell lymphomagenesis through the activation of pre–B-cell-specific molecular-signaling pathways [11]. Therefore, ZFP521 could be considered as a target for molecular-targeted therapy.

3.3. FLT3 signaling and LBL development via Fiz1

(a) Fms like tyrosine kinase 3 (FLT3) belongs to the immunoglobulin superfamily CD135 also known as fetal liver kinase-2 (Flk2). This protein is the receptor for the cytokine Flt3 ligand (FLT3L). FLT3 is a type III receptor tyrosine kinase with five immunoglobulin-like motifs in the extracellular region. In the intracellular region, a tyrosine kinase region (TK) and a C-terminal region composed of a juxtamembrane region (JM) and a kinase insert are contained. This protein is constitutively expressed in the hematopoietic stem and progenitor cells. On the other hand, the ligands that bind to the FLT3 receptor (FL) are produced in bone marrow stromal cells. FL directly stimulates hematopoietic stem cells or together with other cytokines and plays an important role in its survival, proliferation, and differentiation. FLT3 is also one of the critical developmental factors for B- and T-lymphocyte development [40].

In the absence of FL, FLT3 remains in the inactivated monomeric form. When FLT3 binds to FL, a ternary complex is formed in which two FLT3 molecules are bridged by one (homodimeric) FLT3L. Ligand binding promotes conformational changes in FLT3 for dimerization, phosphorylation, and association with adaptor proteins such as Fiz1. The complex formation brings the intracellular domains close to each other, promoting initial phosphorylation of the kinase domain. Activated dimeric FLT3 transduces signals to the downstream effectors. In the pathogenesis analysis, FLT3 is expressed on the cell surface of most AML and ALL cells through proliferation activation and apoptosis suppression, which are caused by the stimulation of FL [4143].

Internal tandem duplications (ITDs) occur in exon 14 or 15 of the JM, which are located directly between the transmembrane domain (TM) and tyrosine kinase region TK1 [44]. Insertions, deletions, and point mutations are frequently found in exon 20 of another tyrosine kinase region TK2.The functional kinase region is kept, and only the JM region is elongated. ITDs probably promote ligand-independent dimerization and activation of FLT3 by changing the conformation of the expressed receptor [44, 45]. In addition, another mutation was identified within the kinase activation loop, a part of the functional core. The conformational changes associated with ITDs might change the structure of the receptor such that unique adaptor proteins such as Fiz1 can now dock.

(b) Fiz 1: This gene encodes the zinc finger protein, which interacts with a receptor tyrosine kinase involved in the regulation of hematopoietic and lymphoid cells. This gene product also interacts with a transcription factor that regulates the expression of rod-specific genes in the retina. Fiz1 binds to the catalytic domain of Flt3 but not to c-Kit, Fms, or platelet-derived growth factor receptor [46, 47]. In a part of B-LBL in SL/Kh, Fiz1 is upregulated by MLV genome insertion and interaction with IL-7R pathway is observed. FLT3 stimulation enhances IL-7R signaling cascade by promotion of Stat5a phosphorylation [48]. Therefore, FLT3 and IL-7R signal pathways interact with each other in the development of B-LBL/ALL.

3.4. CD43 and Hipk2 in the development of B-LBL/ALL

HIPK2 is a conserved serine/threonine nuclear kinase that interacts with homeodomain transcription factors. This protein interacts with the cytoplasmic domain of CD43, which is expressed on immature pro- to pre–B cells, Fr. A-C in Hardy classification. In this immature stage, IL-7R is highly expressed and the CD43 pathway may interact with IL-7R pathway recruiting STAT5A. Hipk2 promotes Wnt signaling by stabilizing beta-catenin [49]. Hipk2 interacts with lymphoid-enhancing factor 1, which acts as a transcriptional factor, promoting c-Myc and cyclin D1 expression [50]. CD43 is an E-selectin counter-receptor highly expressed in human pre–B-cell leukemia NALL-1 cell line [51]. In our study, CD43 cross-linking resulted in an increase in STAT5A phosphorylation, when IL-7 was supplied. CD43 signaling may enhance the IL-7R signal pathway [48, 52].

4. Signaling pathway network responsible for pre–B lymphomagenesis

Probably, multiple genes are related to the activation of IL-7R-signaling pathway. Hipk2 and Fiz 1 are candidates of interaction with IL-7R pathway as well as Stat5. Considering the activation of FLT3 pathway in AML, B-LBL may share the activation pathway with AML [10]. We propose a scheme of interactions among the IL-7-, CD43-, and FLT3-signaling pathways (Figure 8) [48]. Thus, we hypothesize that these three pathways form an interacting network and affect B-LBL development. By contrast, pre-BCR pathway is activated by Zfp521 through the upregulation of BLNK [53, 54], BANK1 [37], Btk, and other pre–BCR-related molecules. Pre-BCR pathway has been considered to contribute to pre–B-cell development rather than to proliferation. Therefore, although stimulation of pre-BCR promotes pre-B cell proliferation, Zfp521 may not directly contribute to lymphomagenesis, but contribute to the stabilization of phenotype of B-LBL. Or interaction with IL-7R and pre-BCR may promote aberrant proliferation or development. Further research is required for precise understanding of the interaction between these two pathways in B-cell development.


Figure 8.

Signaling pathway network in association with IL-7R.


1 - Arber DA, Orazi A, Hasserjian R, Thiele J, Borowitz MJ, Le Beau MM, et al. The 2016 revision to the World Health Organization classification of myeloid neoplasms and acute leukemia. Blood. 2016;127:2391−2405
2 - Swerdlow SH, Campo E, Pileri SA, Harris NL, Stein H, Siebert R, et al. The 2016 revision of the World Health Organization classification of lymphoid neoplasms. Blood. 2016;127:2375−2390
3 - Fugmann SD, Lee AI, Shockett PE, Villey IJ, Schatz DG. The RAG proteins and V(D)J recombination: Complexes, ends, and transposition. Annual Review of Immunology. 2000;18:495−527
4 - Geier JK, Schlissel MS. Pre-BCR signals and the control of Ig gene rearrangements. Seminars in Immunology. 2006;18:31−39
5 - Hendriks RW, Middendorp S. The pre-BCR checkpoint as a cell-autonomous proliferation switch. Trends in Immunology. 2004;25:249−256
6 - Berger A, Hoelbl-Kovacic A, Bourgeais J, Hoefling L, Warsch W, Grundschober E, et al. PAK-dependent STAT5 serine phosphorylation is required for BCR-ABL-induced leukemogenesis. Leukemia. 2014;28:629−641
7 - Berger A, Sexl V, Valent P, Moriggl R. Inhibition of STAT5: A therapeutic option in BCR-ABL1-driven leukemia. Oncotarget. 2014;5:9564−9576
8 - Guo Y, Fan BL, Chen YM, Hu Y, Zou Y, Chen XJ, et al. Analysis of cells in Tel/aml-1 positive childhood acute lymphoblastic leukemia by two-dimensional gel electrophoresis. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2009;17(5):1163−1167. PubMed PMID: 19840443
9 - Fronkova E, Madzo J, Zuna J, Reznickova L, Muzikova K, Hrusak O, et al. TEL/AML 1 real-time quantitative reverse transcriptase PCR can complement minimal residual disease assessment in childhood ALL. Leukemia. 2005;19:1296−1297
10 - Sazawal S, Bhatia K, Gutierrez MI, Saxena R, Arya LS, Bhargava M. Paucity of TEL-AML 1 translocation, by multiplex RT-PCR, in B-lineage acute lymphoblastic leukemia (ALL) in Indian patients. American Journal of Hematology. 2004;76:80−82
11 - Hiratsuka T, Takei Y, Ohmori R, Imai Y, Ozeki M, Tamaki K, et al. ZFP521 contributes to pre-B-cell lymphomagenesis through modulation of the pre-B-cell receptor signaling pathway. Oncogene. 2016;35:3227−3238
12 - Tsuruyama T, Hiratsuka T, Yamada N. Hotspots of MLV integration in the hematopoietic tumor genome. Oncogene. 2017;36:1169−1175
13 - Hiai H, Tsuruyama T, Yamada Y. Pre-B lymphomas in SL/Kh mice: A multifactorial disease model. Cancer Science. 2003;94:847−850
14 - Hiai H, Yamada Y, Abujiang P, Lu L, Kamoto T, Tsuruyama T. Genetic and epigenetic susceptibility to endogenous retrovirus-induced lymphomas in SL mice. Progress in Experimental Tumor Research. 1999;35:64−77
15 - Abujiang P, Yamada Y, Haller O, Kobayashi H, Kamoto T, Lu LM, et al. The origin of SL family mice. Laboratory Animal Science. 1996;46:410−417
16 - Akagi K, Suzuki T, Stephens RM, Jenkins NA, Copeland NG. RTCGD: Retroviral tagged cancer gene database. Nucleic Acids Research. 2004;32:D523−D527
17 - Kaszynski RH, Akatsuka S, Hiratsuka T, Jin G, Ozeki M, Okuno T, et al. A quantitative trait locus responsible for inducing B-cell lymphoblastic lymphoma is a hotspot for microsatellite instability. Cancer Science. 2010;101:800−805
18 - Bowman EP, Campbell JJ, Soler D, Dong Z, Manlongat N, Picarella D, et al. Developmental switches in chemokine response profiles during B cell differentiation and maturation. Journal of Experimental Medicine. 2000;191:1303−1318
19 - Hardy RR, Carmack CE, Shinton SA, Kemp JD, Hayakawa K. Resolution and characterization of pro-B and pre-pro-B cell stages in normal mouse bone marrow. Journal of Experimental Medicine. 1991;173:1213−1225
20 - Hiratsuka T, Tsuruyama T, Kaszynski R, Kometani K, Minato N, Nakamura T, et al. Bone marrow pre-B expansion by SL/Kh-Bomb1 locus: Not sufficient for lymphomagenesis. Leukemia Research. 2008;32:309−314
21 - Lu LM, Shimada R, Higashi S, Zeng Z, Hiai H. Bone marrow pre-B-1 (Bomb1): A quantitative trait locus inducing bone marrow pre-B-cell expansion in lymphoma-prone SL/Kh mice. Cancer Research. 1999;59:2593−2595
22 - Paige CJ, Marshall AJ, Fleming HE, Wu GE. Modulation of the IL-7 dose-response threshold during pro-B cell differentiation is dependent on pre-B cell receptor expression. Journal of Immunology. 1998;161:6038−6045
23 - Wei CJ, Zeff R, Goldschneider I. Murine pro-B cells require IL-7 and its receptor complex to up-regulate IL-7R alpha, terminal deoxynucleotidyltransferase, and c-mu expression. Journal of Immunology. 2000;164:1961−1970
24 - Ihle JN, Schwaller J, Parganas E, Wang DM, Cain D, Aster JC, et al. Stat5 is essential for the myelo- and lymphoproliferative disease induced by TEL/JAK2. Molecular Cell. 2000;6:693−704
25 - Valle-Mendiola A, Weiss-Steider B, Rocha-Zavaleta L, Soto-Cruz I. IL-2 enhances cervical cancer cells proliferation and JAK3/STAT5 phosphorylation at low doses, while at high doses IL-2 has opposite effects. Cancer Investigation. 2014;32:115−125
26 - Natarajan K, Xie Y, Burcu M, Linn DE, Qiu Y, Baer MR. Pim-1 kinase phosphorylates and stabilizes 130 kDa FLT3 and promotes aberrant STAT5 signaling in acute myeloid leukemia with FLT3 internal tandem duplication. PLoS One. 2013;8:e74653
27 - Matsumura I, Kitamura T, Wakao H, Tanaka H, Hashimoto K, Albanese C, et al. Transcriptional regulation of the cyclin D1 promoter by STAT5: Its involvement in cytokine-dependent growth of hematopoietic cells. EMBO Journal. 1999;18:1367−1377
28 - Nosaka T, Kawashima T, Misawa K, Ikuta K, Mui ALF, Kitamura T. STAT5 as a molecular regulator of proliferation, differentiation and apoptosis in hematopoietic cells. EMBO Journal. 1999;18:4754−4765
29 - Tsuruyama T, Hiratsuka T, Jin G, Imai Y, Takeuchi H, Maruyama Y, et al. Murine leukemia retrovirus integration induces the formation of transcription factor complexes on palindromic sequences in the signal transducer and activator of transcription factor 5a gene during the development of pre-B lymphomagenesis. American Journal of Pathology. 2011;178:1374−1386
30 - Copeland NG, Warming S, Liu P, Suzuki T, Akagi K, Lindtner S, et al. Evi3, a common retroviral integration site in murine B-cell lymphoma, encodes an EBFAZ-related Kruppel-like zinc finger protein. Blood. 2003;101:1934−1940
31 - Hentges KE, Weiser KC, Schountz T, Woodward LS, Morse HC, Justice MJ. Evi3, a zinc-finger protein related to EBFAZ, regulates EBF activity in B-cell leukemia. Oncogene. 2005;24:1220−1230
32 - Yamasaki N, Miyazaki K, Nagamachi A, Koller R, Oda H, Miyazaki M, et al. Identification of Zfp521/ZNF521 as a cooperative gene for E2A-HLF to develop acute B-lineage leukemia. Oncogene. 2010;29:1963−1975
33 - Correa D, Hesse E, Seriwatanachai D, Kiviranta R, Saito H, Yamana K, et al. Zfp521 is a target gene and key effector of parathyroid hormone-related peptide signaling in growth plate chondrocytes. Developmental Cell. 2010;19:533−546
34 - Seriwatanachai D, Densmore MJ, Sato T, Correa D, Neff L, Baron R, et al. Deletion of Zfp521 rescues the growth plate phenotype in a mouse model of Jansen metaphyseal chondrodysplasia. FASEB Journal. 2011;25:3057−3067
35 - Shen S, Pu J, Lang B, McCaig CD. A zinc finger protein Zfp521 directs neural differentiation and beyond. Stem Cell Research & Therapy. 2011;2:20
36 - Hesse E, Saito H, Kiviranta R, Correa D, Yamana K, Neff L, et al. Zfp521 controls bone mass by HDAC3-dependent attenuation of Runx2 activity. Journal of Cell Biology. 2010;191:1271−1283
37 - Bernal-Quiros M, Wu YY, Alarcon-Riquelme ME, Castillejo-Lopez C. BANK1 and BLK act through phospholipase C gamma 2 in B-cell signaling. PLoS One. 2013;8(3):e59842
38 - Rickert RC. New insights into pre-BCR and BCR signalling with relevance to B cell malignancies. Nature Review Immunology. 2013;13:578−591
39 - Mullighan CG, Goorha S, Radtke I, Miller CB, Coustan-Smith E, Dalton JD, et al. Genome-wide analysis of genetic alterations in acute lymphoblastic leukaemia. Nature. 2007;446:758−764
40 - Li LX, Goetz CA, Katerndahl CD, Sakaguchi N, Farrar MA. A Flt3- and Ras-dependent pathway primes B cell development by inducing a state of IL-7 responsiveness. Journal of Immunology. 2010;184:1728−1736
41 - Daver N, Strati P, Jabbour E, Kadia T, Luthra R, Wang S, et al. FLT3 mutations in myelodysplastic syndrome and chronic myelomonocytic leukemia. American Journal of Hematology. 2013;88:56−59
42 - Dicker F, Haferlach C, Sundermann J, Wendland N, Weiss T, Kern W, et al. Mutation analysis for RUNX1, MLL-PTD, FLT3-ITD, NPM1 and NRAS in 269 patients with MDS or secondary AML. Leukemia. 2010;24:1528−1532
43 - Georgiou G, Karali V, Zouvelou C, Kyriakou E, Dimou M, Chrisochoou S, et al. Serial determination of FLT3 mutations in myelodysplastic syndrome patients at diagnosis, follow up or acute myeloid leukaemia transformation: Incidence and their prognostic significance. British Journal of Haematology. 2006;134:302−306
44 - Gerloff D, Grundler R, Wurm AA, Brauer-Hartmann D, Katzerke C, Hartmann JU, etal. NF-kappaB/STAT5/miR-155 network targets PU.1 in FLT3-ITD driven acute myeloid leukemia. Leukemia. 2015;29:535−547
45 - Yoshimoto G, Miyamoto T, Jabbarzadeh-Tabrizi S, Iino T, Rocnik JL, Kikushige Y, et al. FLT3-ITD up-regulates MCL-1 to promote survival of stem cells in acute myeloid leukemia via FLT3-ITD-specific STAT5 activation. Blood. 2009;114:5034−5043
46 - Wolf I, Rohrschneider LR. Fiz1, a novel zinc finger protein interacting with the receptor tyrosine kinase Flt3. Journal of Biological Chemistry. 1999;274:21478−21484
47 - Vempati S, Reindl C, Wolf U, Kern R, Petropoulos K, Naidu VM, et al. Transformation by oncogenic mutants and ligand-dependent activation of FLT3 wild-type requires the tyrosine residues 589 and 591. Clinical Cancer Research. 2008;14:4437−4445
48 - Tsuruyama T, Imai Y, Takeuchi H, Hiratsuka T, Maruyama Y, Kanaya K, et al. Dual retrovirus integration tagging: Identification of new signaling molecules Fiz1 and Hipk2 that are involved in the IL-7 signaling pathway in B lymphoblastic lymphomas. Journal of Leukocyte Biology. 2010;88:107−116
49 - Tan M, Gong H, Zeng Y, Tao L, Wang J, Jiang J, et al. Downregulation of homeodomain-interacting protein kinase-2 contributes to bladder cancer metastasis by regulating Wnt signaling. Journal of Cell Biochemistry. 2014;115(10):1762−1767. DOI: 10.1002/jcb.24842. PubMed PMID: 24824041
50 - Wei G, Ku S, Ma GK, Saito S, Tang AA, Zhang J, et al. HIPK2 represses beta-catenin-mediated transcription, epidermal stem cell expansion, and skin tumorigenesis. Proceedings of the National Academy of Sciences United States of America. 2007;104:13040−13045
51 - Nonomura C, Kikuchi J, Kiyokawa N, Ozaki H, Mitsunaga K, Ando H, et al. CD43, but not P-selectin glycoprotein ligand-1, functions as an E-selectin counter-receptor in human pre-B-cell leukemia NALL-1. Cancer Research. 2008;68:790−799
52 - Mao JH, Wu D, Kim IJ, Kang HC, Wei G, Climent J, et al. Hipk2 cooperates with p53 to suppress gamma-ray radiation-induced mouse thymic lymphoma. Oncogene. 2012;31:1176−1180
53 - Baba Y, Hashimoto S, Matsushita M, Watanabe D, Kishimoto T, Kurosaki T, et al. BLNK mediates Syk-dependent Btk activation. Proceedings of the National Academy of Sciences United States of America. 2001;98:2582−2586
54 - Imai C, Ross ME, Reid G, Coustan-Smith E, Schultz KR, Pui CH, et al. Expression of the adaptor protein BLNK/SLP-65 in childhood acute lymphoblastic leukemia. Leukemia. 2004;18:922−925