Antioxidant activities of selected Nigerian plants.
Abstract
The search for natural antioxidants from plants would continue to be a dominant research interest for many years. This is because of the increasing understanding on the role of oxidative stress in damaging cell structures such as DNA, due to over production of free radicals and reactive oxygen species (ROS) in human systems, which are linked to inflammation, cancer and diabetes. However, phenolic compounds especially from phytochemicals or vegetable foods play important roles in reducing the risk of these diseases and reinforces the importance of natural antioxidants in human health. These antioxidant molecules neutralize or quench the ROS by either hydrogen atom transfer or single electron transfer mechanisms. Thus, the capacity to scavenge ROS and free radicals or inhibits lipid peroxidation is measured quantitatively as the strength of antioxidant activity. Several chemical and biochemical protocols have been used in the evaluation of plant extracts as antioxidants. Overwhelming literature reports have indicated varying degrees of antioxidant efficacies of extracts from Nigerian medicinal plants in comparison to synthetic antioxidants. These efficacies were analyzed to provide insight into the strength of antioxidant activity. This chapter reviewed 250 Nigerian medicinal plants in search of evidence for effective antioxidants.
Keywords
- Nigerian medicinal plants
- antioxidants
- DPPH
- ROS
- free radicals
1. Introduction
Since the discovery of enzyme superoxide dismutase (SOD) and the evidence that emerged in support of the role of free radicals in biological systems, human understanding of free radical biochemistry in health and disease continue to advance [1]. This provided the basis for continuous search on natural antioxidants from foods and phytomedicines. Overwhelming reports on Google search engine has indicated 92,800 hits for “antioxidant activity” of medicinal plants in the last 10 years (2008–2018). This is due to growing interest on the antioxidant properties of medicinal plants. Several chemical and biochemical protocols have been used in the evaluation of antioxidant activity including the oxygen radical absorbance capacity (ORAC), total radical-trapping antioxidant potential (TRAP), total oxidant scavenging capacity (TOSC), chemiluminescence (CL), croton bleaching, low density lipoprotein (LDL) oxidation, ferric reducing antioxidant power (FRAP), copper reduction assay (CUPRAC), 2,2′ azinobis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO), hydroxyl radical (OH) hydrogen peroxide (H2O2) and total phenolic assay among others [2]. Biochemical protocols are based on animal models for
2. Reactive oxygen species (ROS) in human health and disease
Human system uses oxidation for normal metabolic activities in the transformation of nutrients into energy. During oxidation, reactive oxygen species (ROS) are also produced at low levels in normal physiological conditions, which are necessary for maintaining normal cell functions such as signaling immunity and homeostasis [4]. These activities are maintained by endogenous antioxidant (enzymatic) defense systems produced by the body for protection against harmful effects. These include superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione reductase (GSH-Rx) and catalase [5]. Excessive production of ROS beyond the body defense mechanisms can be extremely harmful to cellular functions by damaging nucleic acids, oxidizing proteins, and causing lipid peroxidation [6]. The resultant cell damage by free radicals and ROS appeared as major contributor to aging and degenerative diseases of aging such as cancer, cardiovascular disease, immune system decline, liver diseases, diabetes mellitus, inflammation and brain dysfunction among others [7, 8]. These ROS and reactive nitrogen species (RNS) including superoxide anion O2−*, hydroxide ion OH−*, hydroxyl radical OH*, peroxyl radical ROO* and nitric oxide NO* as well as H2O2, lipid peroxides ROOH, and singlet O2 are very reactive and can initiates free radical reactions or lipid peroxidation in living cells.
ROS can be produced either by external sources (e.g., tobacco smoke, stress, etc.), as by-products during the mitochondrial electron transport of aerobic respiration or by oxidoreductase enzymes and metal catalyzed oxidation [9]. But the biological effects of ROS depend on the types of cell or tissue in relation to enzyme production, signal transduction and DNA repair [10]. ROS are harmful when excessive productions are not balanced by body antioxidant mechanism. This imbalance between ROS production and enzymatic antioxidant defense systems is called oxidative stress [11]. Antioxidants counteract oxidative stress by neutralizing free radicals because they are reducing agents that react with and buffer ROS as a form of defense against oxidative stress [12].
3. Phytochemicals as sources of natural antioxidants
Antioxidants are molecules that prevent oxidation or inactivates the reactive oxygen species and thus prevent oxidative damage to the cells and body tissues [13]. Antioxidants can also inhibit, quench or scavenge free radicals converting them into new and stable chemical compounds [14]. Broadly, antioxidants are classified as enzymatic and non-enzymatic with each class providing complementary role of protection against free radicals in human systems. Previous work has concisely discussed on antioxidants classification [3] as summarily reproduced in Figure 1. But our focus is the non-enzymatic antioxidant involving flavonoids, phenolic acids, vitamins, carotenoids, minerals and cofactors. They are exogenous sources of protection through diet. Plants foods contain a variety of nutrients and non-nutrients chemicals which are good antioxidant agents. These sources of natural antioxidants including Vitamin A (retinol) obtained from β-carotene, vitamin C (ascorbic acid), Vitamin E (α-tocopherol), lycopene and carotenoids occur naturally in fruits, vegetables, legumes and grains which are commonly consumed and play important role in the defense against free radicals [3, 15]. Medicinal plants are rich source of phenolic compounds such as flavonoids, phenolic acids and coumarins [16, 17]. Flavonoids are antioxidants compounds composed of anthocyanins, flavanones, flavonols, flavones, isoflavonoids and flavanones, while hydroxycinnamic and hydroxybenzoic acids such as gallic acid are components of phenolic acids widely distributed secondary metabolites in plants with antioxidant and antiradical properties [18]. They are important as chelators and free radical scavengers of hydroxyl and peroxyl radicals, superoxide anions and peroxynitrites [19]. Carotenoids natural pigments are important phytochemical antioxidants obtained from plants. They are structurally grouped into carotenes and xanthophyll based on the degree of oxygenation of carotenoid hydrocarbons and exert antioxidant effect by singlet oxygen quenching ability [3]. Several studies on the antioxidant activities of various herbal plants have indicated their enormous medicinal values as inhibitors of free radical and ROS [20].
4. Antioxidants from Nigerian medicinal plants
Nigeria is a west African country with an area of 923,769 km2 having a population of 198 million with 250 ethnic groups [21]. The country shares border with republic of Cameroun to the east, Niger and Chad republics to the north, Benin republic to the west and Gulf of Guinea to the south. Nigeria has favorable climate conditions with enormous diversity of plant species, which are distributed across geographical contrast of the mangrove swamps in South-South (SS), to the tropical rain forests covering South-West (SW) and South-East (SE) and to the grassland vegetation of North-Central (NC) up to the Sahel savannah of semi-arid North-East (NE) (Figure 2). Many of these plants are used as medicines for treatment of illness or management of human and animal health among rural and urban dwellers.
The application of herbal recipes especially in the management of human metabolic diseases such as diabetes and cancer is common knowledge among Nigerians. This prompted research interest in academia on the potentials of phytomedicines as complimentary or alternative treatment agents, and consequent research efforts to validate their pharmacological properties. The number of Nigerian medicinal plants reported for antioxidants is enormous. However, 250 medicinal plants evaluated for antioxidant activity were studied in addition to the 28 compounds isolated from 44 plants. But antioxidant evaluations on crude extracts rather than on pure compounds largely dominated the literature. Thus, effective activity based on concentrations required to inhibit 50% free radicals (IC50) for selected extracts are presented (Table 1) together with concentrations of various standard antioxidants used.
S. No | Plant name | Family | Part used | IC50 sample | IC50 standard | Ref. |
---|---|---|---|---|---|---|
1 | Seed | 1.92* 2.10* |
AA = 1.83 AA = 1.20 |
[37] | ||
2 | Euphorbiaceae | Leaf | 20.50* | TC = 15.4 | [68] | |
3 | Euphorbiaceae | Leaf | 15.25* | AA = 7.26 | [26] | |
4 | Asteraceae | Aerial | 28.9* | AA = 1.41 | [39] | |
5 | Zingiberaceae | Fruit Leaf |
0.04** 0.07** |
AA = 0.03 | [69] | |
6 | Asteraceae | Leaf | 31.25* | AA = 7.26 | [26] | |
7 | Apocynaceae | Leaf | 0.46** | VE = 0.25 | [70] | |
8 | Guttiferae | Leaf | 0.02** 0.1** |
VE = 0.01 | [71] | |
9 | Apocynaceae | Stem | 0.12** | AA = 0.06 | [72] | |
10 | Apocynaceae | Root | 19.7* | AA = 4.9 | [73] | |
11 | Amaranthaceae | Leaf | 35* | AA = 125 | [46] | |
12 | Amaranthaceae | Leaf Stem |
15.81** | TC = 13.2 | [27] | |
13 | Anacardiaceae | Bark Leaf |
5.66* 7.77* |
AA = 4.57 | [74] | |
14 | Annonaceae | Leaf | 45.72* 49.0* |
GA = 48.77 TX = 72.9 |
[35] | |
15 | Asteraceae | Leaf | 160* | AA = 120 | [75] | |
16 | Acanthaceae | Leaf | 100* | AA = 150 | [75] | |
17 | Caesalpiniaceae | Leaf | 20.52* | AA = 19.8 | [76] | |
18 | Caesalpiniaceae | Leaf | 5.56* | AA = 30.0 | [45] | |
19 | Bixaceae | Leaf | 0.45* | VE = 0.25 | [70] | |
20 | Rubiaceae | Aerial | 1.85** | AA = 0.05 | [77] | |
21 | Rubiaceae | Leaf | 2.98* | AA = 1.05 | [78] | |
22 | Euphorbiaceae | Leaf | 12.5* | AA = 7.26 | [26] | |
23 | Euphorbiaceae | Leaf | 0.1 μM | AA = 2.0 μM | [38] | |
24 | Crassulaceae | Leaf | 0.41** | VC = 0.067 | [79] | |
25 | Mimosaeae | Flower | 28** | VE = 38 | [80] | |
26 | Orchidaceae | Whole | 50.6* | AA = 1.41 | [39] | |
27 | Rubiaceae | Leaf | 23.9* | AA = 4.9 | [74] | |
28 | Solanaceae | Fruit | 1.15** | BHA = 0.96 | [25] | |
29 | Solanaceae | Fruit | 0.67** | BHA = 0.96 | [25] | |
30 | Caricaceae | Seed | 0.227** | AA = 0.109 | [67] | |
31 | Leguminosae | Leaf | 24.1* 46.6* |
AA = 4.9 TC = 38.9 |
[73] | |
32 | Leguminosae | Leaf | 1.20* | AA = 2.56 | [48] | |
33 | Amaranthaceae | Leaf | 120* | AA = 120 | [75] | |
34 | Menispermaceae | Leaf | 2.77* | AA = 0.067 | [25] | |
35 | Rutaceae | Peel | 12.1** | VC = 0.067 | [25] | |
36 | Burseraceae | Leaf | 0.33** 0.54** |
AA = 0.49 | [23] | |
37 | Malvaceae | Leaf | 11.8** 27.52* |
TC = 13.2 AA = 188.3 |
[27] [33] |
|
38 | Asteraceae | Leaf | 2.91** 1.73** |
VC = 1.18 VC = 0.56 |
[62] | |
39 | Cucurbitaceae | Leaf | 1.68** 71.1** |
BHA = 0.96 TC = 13.2 |
[27] [25] |
|
40 | Cucurbitaceae | Leaf | 150* | AA = 120 TC = 50 |
[75] | |
41 | Poaceae | Leaf | 1.35* | VE = 0.25 | [70] | |
42 | Leguminosae | Leaf | 15.5* | TC = 0.25 | [50] | |
43 | Apiaceae | Aerial | 4.61** | BHA = 0.96 | [25] | |
44 | Boraginaceae | Leaf | 0.47** | GA = 2.09 | [53] | |
45 | Asteraceae | Leaf | 120* | AA = 120 | [75] | |
46 | Myrtaceae | Leaf Bud |
0.03* 0.02* |
AA = 0.03 | [47] | |
47 | Asteraceae | Root | 22.4* 53.7* |
AA = 4.9 RT = 3.3 |
[73] | |
48 | Asteraceae | Leaf | 0.07** | AA = 0.06 | [72] | |
49 | Euphorbiaceae | Leaf | 2.5** | VC = 4.5 | [81] | |
50 | Rubiaceae | Root | 0.053** | VC = 0.048 | [43] | |
51 | Moraceae | Leaf | 0.86* | VE = 0.25 | [70] | |
52 | Moraceae | Leaf | 45.3* 44.6* |
GA = 48.8 TX = 72.9 |
[34] | |
53 | Moraceae | Stem | 42.0* | VC = 25.0 | [82] | |
54 | Loranthaceae | Leaf | 0.38** | VC = 0.06 | [79] | |
55 | Asclepiadaceae | Leaf | 70.0* | VC = 50 | [83] | |
56 | Tiliaceae | Leaf Stem |
0.32** 0.39** |
AA = 0.31 AA = 0.18 |
[32] | |
57 | Hypericaceae | Stem | 37.5* | BHT = 16.2 | [36] | |
58 | Boraginaceae | Aerial | 48.4* | AA = 1.41 | [39] | |
59 | Malvaceae | Leaf | 0.14* | AA = 0.02 | [84] | |
60 | Apocynaceae | Leaf | 7.2* | QT = 2.95 | [85] | |
61 | Convulvulaceae | Leaf | 24.3* | AA = 1.41 | [39] | |
62 | Irvingiaceae | Root Stem |
12.4* 25.5* |
AA = 4.9 TC = 38.9 |
[73] | |
63 | Acanthaceae | Leaf | 1.58 μM | AA = 2.52 | [86] | |
64 | Crassulaceae | Leaf | 180* | AA = 120 | [75] | |
65 | Asteraceae | Whole | 0.26** | QT = 0.83 | [25] | |
66 | Apocynaceae | Root | 8.8* 49.1* |
AA = 4.9 TC = 38.9 |
[73] | |
67 | Urticaceae | Leaf | 100* | AA = 150 | [75] | |
68 | Icacinaceae | Leaf Root |
0.30** 0.27** |
RT = 0.26 | [28] | |
69 | Asteraceae | Shoot Leaf |
1.94** 1.59** |
VC = 1.18 VC = 0.56 |
[62] | |
70 | Lythraceae | Leaf | 3.80* | AA = 7.26 | [26] | |
71 | Asclepiadaceae | Leaf | 42.3* | GA = 48.8 | [35] | |
72 | Solanaceae | Fruit | 1.16** 1.47** |
QT = 0.83 | [25] | |
73 | Rubiaceae | Leaf | 70.0* | VC = 7.59 | [87] | |
74 | Apocynaceae | Leaf | 6.1* | AA = 3.4 | [66] | |
75 | Moringaceae | Leaf | 0.16* | AA = 0.02 | [84] | |
76 | Rutaceae | Leaf | 7.35* | TC = 13.2 | [27] | |
77 | Rubiaceae | Stem | 18.12* | AA = 1.41 | [39] | |
78 | Rubiaceae | Leaf | 12.9* | AA = 4.9 | [73] | |
79 | Lamiaceae | Leaf | 1.0* | AA = 9.0 | [55] | |
80 | Lamiaceae | Leaf Stem |
0.14* 8.67* |
AA = 0.02 BHA = 3.36 |
[33] [27] |
|
81 | Chrysobalanaceae | Leaf | 13.5* | VC = 1.98 | [88] | |
82 | Leguminosae | Stem | 15.65* | AA = 7.26 | [26] | |
83 | Loranthaceae | Leaf | 1.9* 1.0* |
BHT = 4.6 VC = 10 |
[41] | |
84 | Fabaceae | Leaf | 10.3* | AA = 3.9 | [22] | |
85 | Fabaceae | Leaf | 14.7* | AA = 3.9 | [22] | |
86 | Piperaceae | Seed | 74* | AA = 31.7 | [89] | |
87 | Myrtaceae | Leaf | 0.04** | BHA = 0.05 | [24] | |
88 | Euphorbiaceae | Stem | 0.19** | BHT = 0.11 | [90] | |
89 | Fabaceae | Leaf | 0.59** | VC = 0.067 | [79] | |
90 | Simaroubaceae | Stem | 4.7* | BHT = 5.0 | [42] | |
91 | Solanaceae | Leaf | 6.21** | TC = 13.2 | [27] | |
92 | Amaranthaceae | Leaf | 12.6* | TC = 13.2 | [27] | |
93 | Anacardiaceae | Stem | 8.3* | AA = 11.5 | [52] | |
94 | Verbenaceae | Leaf | 5.0* | AA = 9.0 | [51] | |
95 | Apocynaceae | Root | 1.18** | VC = 0.067 | [79] | |
96 | Cucurbitaceae | Leaf | 0.16** | AA = 0.02 | [84] | |
97 | Meliaceae | Stem | 30.28* | AA = 20.72 | [64] | |
98 | Asteraceae | Leaf | 31.25* | AA = 7.26 | [26] | |
99 | Asteraceae | Leaf | 1.90 μM | AA = 2.0 μM | [49] | |
100 | Asteraceae | Leaf | 6.50* 8.0* |
GA = 0.62 | [30] | |
101 | Asteraceae | Leaf | 20.0* | AA = 18.0 | [91] | |
102 | Verbenaceae | Leaf | 53.23* | GA = 48.8 | [34] | |
103 | Annonaceae | Fruit | 1.04** | VC = 0.067 | [79] | |
104 | Zingiberaceae | Rhizome | 47.0* | AA = 36.4 | [92] |
5. Antioxidant activities of crude extracts
The antioxidant efficacies of Nigerian plants were largely evaluated using protocols involving DPPH, ABTS, FRAP, TAC, NO, OH and or H2O2 targets. The DPPH radical scavenging assay is one of the commonly used techniques for quick evaluation of antioxidant capacity. Plant extracts tested for DPPH inhibition have demonstrated interesting efficacies for instance, crude extracts of
The analysis of antioxidant efficacies on medicinal plants reported from 2013 to 2017 in 55 publications, involving 211 extracts from 144 plants was carried out. We observed that 70 extracts from 50 plants have exhibited good antioxidant efficacies on various free radical targets with 51 extracts from 53 plants having comparable efficacies to standard antioxidants. However, lower IC50 or higher percent (%) inhibitions compared to standards were observed with 20 extracts from 17 medicinal plants. The NO● inhibition on root extract of
Furthermore, DPPH inhibitions on
6. Chemical composition and antioxidant activity
6.1 GC-MS analysis of extracts and evaluation of antioxidant activity
The antioxidant evaluations of Nigerian medicinal plants with determination of chemical composition using gas chromatography-mass spectrometry (GC-MS) have become routine studies. The GC-MS is intended to give insight on the probable chemical entities of volatile components present in the sample extract. Several plants constituents have been analyzed using GC–MS by comparison of compounds’ retention times with library of standard chemical entities provided by the National Institute of Standards and Technology (NIST) database imbedded in the instrument. The chemical constituents with low molecular weights such as terpenoids, long chain alkanes, phenolics and fatty acid methyl esters (FAME) are separated and detected by GC-MS. This is perhaps one reason that FAME are prevalent from among plant extracts, but sharp contrast between lipophilic and hydrophilic components are determined by solvent polarity or method of extraction [44].
The GC-MS analyses and evaluation of antioxidants on
The leaf ethyl acetate extract of
6.2 GC-MS analysis of essential oils and evaluation of antioxidant activity
The essential oils (EO) from Nigerian medicinal plants have been analyzed using the GC–MS and evaluated for antioxidants activity. Because they are mixtures of several constituents containing largely low molecular weights compounds, EO are rapidly analyzed using GC–MS to ascertain their chemical composition. The essential oils (EO) from
6.3 HPLC analysis of extracts and evaluation of antioxidant activity
The high-performance liquid chromatography (HPLC) has been reported in the analysis of major chemical constituents of plant extracts alongside with the antioxidant activity. The HPLC technique uses reverse phase chromatography because of simplicity, versatility and sensitivity towards separation, purification, quantification and identification of diverse natural products such as plant phenolics, steroids, alkaloids and flavonoids [60]. Hence, the combination of HPLC methods with antioxidants evaluations may provide the needed understanding of antioxidant efficacies of plant extracts. Previous HPLC profiling of ethanol extract of
Although antioxidant activities of plant extracts using DPPH have been established to correlate with phenolics and flavonoids contents [63]. However, many of the plants evaluated for antioxidants activity have no correlation with the number and amounts of phenolics and flavonoids quantified by HPLC. The report on
7. Antioxidant activities of isolated compounds
The antioxidant evaluations on isolated compounds from Nigerian medicinal plants are rarely reported. This is probably due to funding problems associated to plant chemistry research in Nigeria, coupled with dysfunctional analytical instruments such as the NMR spectrometer. Most of the published research on isolation and characterization of compounds were carried out abroad. Of the 250 plants analyzed for antioxidant evaluations, only 28 compounds were isolated from 44 plants together with full spectral characterization. The antioxidant activities of quercetin and quercetin-3-
S. No | Chemical name | Plant | Model | Compd. (IC50) | Stand. (IC50) | Ref. |
---|---|---|---|---|---|---|
1 | Quercetin | DPPH | 10.64^ | AA = 12.52 | [93] | |
2 | Quercetin-3-O-rutinoside | DPPH | 16.11^ | AA = 12.52 | [93] | |
3 | Isovitexin | DPPH | 189.1^ | QT = 5.31 | [98] | |
4 | Trans-ethyl-3-(3, 4-dihydroxyphenyl acrylate | DPPH | 14.49^ | AA = 13.18 | [99] | |
5 | p-hydroxy benzaldehyde | DPPH | 73.50* | VC = 37.5 | [102] | |
6 | Tiliroside | DPPH | 360.1* | AA = 70.12 | [100] | |
7 | Isovitexin | DPPH | 211.6* | AA = 70.12 | [100] | |
8 | Helichrysoside-3′-methyl ether | DPPH | 183.4* | AA = 70.12 | [100] | |
9 | Betulin | DPPH | >100* | VC = 1.98 | [88] | |
10 | β-sitosterol | DPPH | >50* | VC = 1.98 | [88] | |
11 | Betulinic acid | DPPH | >100* | VC = 1.98 | [88] | |
12 | 4-(3′,3-dihydroxyl-1-mercaptopropyl) phenyl-glucosylpyranoside | DPPH | 75* | VC = 7.59 | [87] | |
13 | Agathisflavone | DPPH | 366.4* | AA = 4.57 | [74] | |
14 | Quercetin-3-O-rutinoside/rhamnoside | DPPH | 0.96* | AA = 4.57 | [74] | |
15 | Rosmarinic acid | DPPH | 4.99* | QT = 2.32 | [97] | |
16 | Methyl rosmarinate | DPPH | 5.97* | QT = 2.32 | [97] | |
17 | Caffeic acid | DPPH | 3.03* | QT = 2.32 | [97] | |
18 | Methyl caffeate | DPPH | 13.41* | QT = 2.32 | [97] | |
19 | Apigenin | DPPH | 26.67* | QT = 2.32 | [97] | |
20 | Luteolin | DPPH | 5.35* | QT = 2.32 | [97] | |
21 | Apigenin glucuronide | DPPH | 185.89* | QT = 2.32 | [97] | |
22 | Epicatechin | DPPH | 19.02^ | GA = 12.82 | [101] | |
23 | Epigallocatechin | DPPH | 15.88^ | GA = 12.82 | [101] | |
24 | Procyanidin B5 | DPPH | 8.80^ | GA = 12.82 | [101] | |
25 | Kaempferol-3-O-rutinoside | FRAP | 394.8* | QT = 2.95 | [85] | |
26 | Quercetin-3-O-glucoside | LPI FRAP |
10.4* 1649.4* |
QT = 2.95 | [85] | |
27 | Kaempferol-3-O-glucoside | FRAP | 337.5* | QT = 2.95 | [85] | |
28 | Quercetin-3-O-glucoside/galactoside mixture (1: 1) | LPI FRAP |
9.8* 1589.9* |
QT = 2.95 | [85] | |
29 | Quercetin | DPPH ABTS |
1.58# 0.81# |
AA = 2.44 TX = 0.81 |
[96] | |
30 | Kaempferol | DPPH | 7.75# | AA = 2.44 | [96] | |
31 | Dihydrokaempferol | DPPH | 82.93# | AA = 2.44 | [96] | |
32 | Piceatannol | DPPH | 3.96# | AA = 2.44 | [96] | |
33 | (−)-Catechin | DPPH H2O2 |
88* 13* |
AA = 6 AA = 8 |
[95] | |
34 | (+)-epicatechin | DPPH H2O2 |
40* 10* |
AA = 6 AA = 8 |
[95] | |
35 | (−)-epicatechin | DPPH H2O2 |
10* 8* |
AA = 6 AA = 8 |
[95] | |
36 | (2R,3R)-dihydroquercetin | DPPH H2O2 |
46* 18* |
AA = 6 AA = 8 |
[95] | |
37 | Catechin | DPPH Fe(II) |
0.03** 1.29** |
AA = 0.01 EDTA = 0.05 |
[15] |
8. Conclusion
Analysis of antioxidant efficacies of Nigerian medicinal plants reported from 1998 to 2018 was carried out. The aim was to provide evidence for effective antioxidants. Our findings have shown the enormous potentials of Nigerian plants as sources of natural antioxidants. We have observed various crude extracts obtained mainly from polar solvents with antioxidant efficacies better than standard compounds. Such preponderance of evidence indicated by broad spectrum of free radical and non-free radical inhibitions has defined the comparable strength of plant extracts to standard antioxidants. Nigerian plants have the capacity to protect or inhibit damage induced by free radical species. This study attempts to provide insights on the strength of antioxidant efficacies of plant extracts comparable to standard antioxidants. However, it is recommended that comprehensive approach to plant bioactive research must be adopted in search of antioxidants to avoid replication of studies especially on certain species. There is need for collaboration among Nigerian scientist working in related areas to enhance on the scope of research questions and improve on the quality of research output.
Acknowledgments
Authors acknowledged the plant taxonomist, Umar Shehu Gallah of National Research Institute for Chemical Technology (NARICT) Zaria, for providing the local (Hausa) names of plants. We are grateful to Ahmadu Bello University, Zaria for providing some of the facilities used during the study.
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