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Biochemistry, Genetics and Molecular Biology » "Ideas and Applications Toward Sample Preparation for Food and Beverage Analysis", book edited by Mark T. Stauffer, ISBN 978-953-51-3686-6, Print ISBN 978-953-51-3685-9, Published: December 13, 2017 under CC BY 3.0 license. © The Author(s).

Chapter 8

Sample Preparation Methods for Pesticide Analysis in Food Commodities, Biological and Environment Matrices

By Renata Raina-Fulton and Zhen Xie
DOI: 10.5772/intechopen.69791

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QuEChERS and modified QuEChERS approaches.
Figure 1. QuEChERS and modified QuEChERS approaches.
Strategies for extraction utilizing solid phase extract for preconcentration or extract cleanup.
Figure 2. Strategies for extraction utilizing solid phase extract for preconcentration or extract cleanup.

Sample Preparation Methods for Pesticide Analysis in Food Commodities, Biological and Environment Matrices

Renata Raina-Fulton and Zhen Xie
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This chapter focuses on sample preparation procedures for pesticide analysis of food commodities, biological and environmental matrices. This will include pesticides with a broad range of polarity including those that are more amenable to gas chromatography-mass spectrometry (organochlorines, organophosphorus pesticides, and pyrethroids) and those commonly analyzed by liquid chromatography-mass spectrometry (carbamates, azole, and strobilurin fungicides, and phenylureas as well as organophosphorus pesticides). QuEChERS (quick, easy, cheap, effective, rugged, and safe) methods or QuEChERS methods with modifications to allow wetting of the dry sample matrix, buffering, changing extraction solvent from acetonitrile to ethyl acetate are examined. Subsequent cleanup using dispersive solid phase extraction or cartridge format solid phase extraction has also been completed to reduce matrix effects. Other solid matrices are frequently extracted with pressurized liquid extraction, microwave assisted extraction, or ultrasonic extraction combined with or followed by dispersive solid phase extraction or solid phase extraction. Particularly for chromatography-mass spectrometry, careful consideration of matrix effects needs to be made when considering the design of the sample preparation procedures. Selection of extraction solvent needs to consider both polarity of target analytes (and their solubility in selected solvents) as well as co-extracted matrix components.

Keywords: QuEChERS (quick, easy, cheap, effective, rugged, and safe) methods, solid phase extraction, gas chromatography-mass spectrometry (GC-MS), gas chromatography-tandem mass spectrometry (GC-MS/MS), liquid chromatography-tandem mass spectrometry (LC-MS/MS), azole fungicides, carbamates, organochlorines, organophosphorus pesticides (OPs), phenylureas, pyrethroids, strobilurin fungicides, metabolites, degradation products

1. Introduction

Gas chromatography-mass spectrometry (GC-MS), gas chromatography-tandem mass spectrometry (GC-MS/MS), and liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods are used to analyze for azoles, carbamates, organophosphorus pesticides, pyrethroids, phenylureas, strobilurin fungicides, and other pesticides in a diverse range of sample matrices including food commodities, biological and environmental matrices. The chromatography-mass spectrometry choices for the analysis of these pesticides and others have been recently reviewed [1, 2]. Briefly, organochlorines (OCs), organophosphorus pesticides (OPs), and pyrethroids are frequently analyzed with GC-MS or GC-MS/MS methods. Analysis of azole fungicides, carbamates, neonicotinoids, phenylureas, and strobilurin fungicides is more often analyzed by LC-MS/MS methods. Use of liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI+-MS/MS) for analysis of OPs has also increased over the last 10 years [1]. This chapter will discuss selection choices for extraction and cleanup of sample extracts or preconcentration of target analytes prior to chemical analysis (chromatography-mass spectrometry methods) to minimize matrix enhancement or suppression observed in MS detection. The options for preconcentration or cleanup of sample extracts also depend upon whether the sample is a liquid or solid matrix, fat content, and water content. Modified QuEChERS and microwave and pressurized solvent extraction remain the most widely used extraction procedures with inclusion or subsequent cleanup using dispersive solid phase extraction (dSPE) or solid phase extraction (SPE) methods and will be the focus of discussions in this chapter.

2. Modified QuEChERS procedures and dispersive solid phase extraction

QuEChERS (quick, easy, cheap, effective, rugged, and safe) methods without buffer or with acetate or citrate buffer or other modified QuEChERS methods remain one of the most popular approaches to sample extraction and cleanup of food commodities (Table 1). This approach has also been applied to other solid sample matrices including bee products and soil as shown in Table 1 [328]. Figure 1 shows a comparison of the typical parameters used in various modified QuEChERS methods. Phase separation and partitioning of target analytes into the organic phase is generally achieved with addition of anhydrous MgSO4 (subsequently noted as MgSO4) and NaCl. Addition of NaCl improves the removal of acetonitrile from the aqueous phase and partitioning of polar analytes into acetonitrile [29]. The salt-out extraction is followed by cleanup of the extract with dispersive solid phase extraction (dSPE). Common dSPE sorbents include C18 or C8 for removal of lipids; florisil for removal of polar and low-fat co-extracts; graphitized carbon black (GCB) for removal of pigments and some fatty acids; primary secondary amine (PSA) for efficient removal of saccharides and organic acid as it is a weak anion exchanger; and Z-Sep (ZrO2 bonded to silica) or Z-Sep+ (ZrO2 and C18 both bonded to silica) for removal of lipids [15]. PSA has been reported to remove butanoic acid, decanoic acid, heptanoic acid, hexanoic acid, linoleic acid, and phytosterol (stigmasterol), while not effectively removing alkaloids (caffeine and theobromine) and γ-tocopherol [30]. The use of GCB with PSA, C18, and anhydrous MgSO4 was found to improve recoveries for OPs and carbamates in egg matrix as compared to when GCB was not used [31].

QuEChERS (solvent, salts)dSPE (solvent)Sample matrixPesticides [recoveries]Analysis methodReference
ACN salt-outPSA:C18:GCB (1:1:1) 50 or 125 mg
(ACN followed by ACN/toluene 3:1)
Pollen and single bumble peesNeonicotinoids (acetamiprid, clothianidin, imidacloprid, thiacloprid, thiamethoxam) [81–87%, pollen; 88–96% bumble bee];
azoles (epoxiconazole, flusilazole, metconazole, tebuconazole, triticonazole) [81–102% pollen; 75–90% bumble bee];
strobilurin fungicides (fluoxastrobin, pyraclostrobin, trifloxystrobin) [71–87%, pollen; 74–82% bumble bee];
others (boscalid, carbendazim, carboxin, prochloraz, spiroxamine) [66–88%, pollen; 63–90% bumble bee].
7.5 mL H2O, 10 mL ACN, 6 g MgSO4, 1 g NaCl15 mg C18, 50 mg PSA, 50 mg MgSO4 per mL of ACN extractHoney beesAzoles (imazalil, prochloraz, tebuconazole, thiabendazole) [77–96%];
Carbamates (carbendazim, carbofuran, methiocarb) [70–95%];
neonicotinoids (acetamiprid, imidacloprid, thiamethoxam) [80–92%];
OPs (azinphos ethyl, azinphos methyl, chlorfenvinphos, chlorpyrifos, coumaphos, diazinon, diclofenthion, dimethoate, ethion, fenitrothion, fenthion, malathion, omethoate, parathion-ethyl, parathion-methyl, triclofos-methyl) [70–95%]; phenylureas (diuron, isoproturon) [82–86%];
pyrethroids (flumethrin, fluvalinate) [84–93%];
triazines (atrazine, simazine, terbumeton, terbuthylazine) [80–91%];
Degradation products (atrazine-desethyl, atrazine-desisopropyl, carbofuran-3-hydroxyl, fenoxon-sulphone [70–75%], fenoxon-sulfoxide, fenthion-sulfone, fenthion-sulfoxide [75–80%], terbumeton-desethyl, terbuthylazine-2-hydroxyl, terbuthylazine-desethyl [75–82%]) [80–94%].
10 mL ACN, 3 mL hexane before salt additionHoney bees
Wetted (10 mL H2O)
OPs (coumaphos, diazinon, dimethoate, heptenophos, methidathion, omethoate, oxydemeton-methyl, profenophos, pyrazophos, temephos) [70–93%]LC-ESI+-MS/MS[5]
ACN, 4 g MgSO4, 1 g NaCl, 1 g Na3citrate dihydrate, 0.5 g Na2Hcitrate sesquihydratePSA (25 mg), 150 mg MgSO4 per mL extractPollenAzole fungicides (bitertanol, bromuconazole, difenoconazole, diniconazole, epoxiconazole, fenbuconazole, flusilazole, flutriafol, hexaconazole, paclobutrazole, penconazole, prochloraz [70%], propiconazole, tetraconazole, etc.) [88–94%];
N-methylcarbamates (carbaryl, formetanate, methomyl, oxamyl, pirimicarb, propoxur) [93–96%];
Neonicotinoids (acetamiprid, clothianidin, imidacloprid, nitenpyram, thiacloprid, thiamethoxam) [average 96 and 107%];
OPs (azinphos methyl, demeton-s-methyl sulfone, diazinon, dicrotophos, dimethoate, ethio, ethoprophos, fenamiphos, fenthion degrades, malaoxon, methamidophos, phenofos, trichlorfon, etc.) [>70%];
Strobilurin fungicides (azoxystrobin, kresoxim-methyl, pyraclostrobin, trifloxystrobin) [77–107%];
Others (2,4-D, cyromazine, ethirimol, fipronil, pymetrozine) [35–66%].
10 mL ACN +3 mL hexane (pollen); 10 mL ACN for corn syrup with citrate buffer50 mg PSA + 50 mg C18 + 150 mg MgSO4Pollen and high fructose corn syrup
Wetted (1:4 dilution)
Neonicotinoids (acetamiprid, clothianidin, dinotefuran, flonicamid, imidacloprid, nitenpyram, thiacloprid, thiamethoxam) [>88–110%].LC-ESI+-MS/MS[7]
ACN, 1% CH3COOH, 6 g MgSO4, 1.5 g NaOAc0.6 g MgSO4, 0.2 g PSATomatoAzoles (bromuconazole, cyproconazole, difenconazole, diniconazole, epoxiconazole, flutriafol, hexaconazole, imazalil, myclobutanil, penconazole, propiconazole, thiaphanate methyl, triadimefon, triadimenol, triflumizole) [92–106%]; Carbamates (carbaryl, carbofuran, chlorpropham, cycloate, diethofencarb, ethiofencarb, fenoxycarb, methomyl, oxamyl, pirimicarb) [85–104%]; OPs (azinphos methyl, chlorpyrifos ethyl, chlorpyrifos methyl, diazinon, dimethoate, ethoprophos, fenthion, malathion, monocrotophos, omethoate, parathion methyl, pirimiphos methyl, prothiofos, thiometon) [83–109%]; strobilurin fungicides (azoxystrobin, kresoxim methyl, trifloxystrobin) [94–104%]; phenyl or benzoyl ureas (diuron; chlorfluazuron, hexaflumuron, lufenuron) [98–106%]; pyrethroids (bifenthrin, cypermethrin, deltamethrin, fenproprathrin) [93–112%]LC-ESI+-MS/MS[8]
ACN, 4 g MgSO4, 1 g NaCl30 mg PSA, 150 mg MgSO4Leaf vegetable (pakchoi, rape, crown daisy, amaranth, spinach, lettuce)Anilide fungicide (metalaxyl) [80–115%];
aryloxyphenoxypropionate herbicide (fluazifop-methyl) [83–119%].
OP (chlorpyrifos) [84–111%]; pyrethroid (Lambda-cyhalothrin) [81–117%].
10 mL ACN, 4 g MgSO4, 1 g NaCl50 mg PSA, 100 mg MgSO4Fruits and vegetables (apple, cabbage, carrot, tomato)Carbamates (aldicarb, baycarb, carbaryl, ethiofencarb, methiocarb); [88–120%]; OPs (azinphos-methyl, malathion, methidathion, pirimiphos-methyl [58–71%], etrimfos, pyraclofos, phosalone) [81–120%];
methiocarb-sulfone [72–87%]
14 mL 1% CH3COOH in ACN, 6 g MgSO4, 1.4 g NaOAc, 4 g NaClAll 900 mg MgSO4 and 150 mg PSA
>5% fat content
also 150 mg C18
<5% colorless to pale extract color, no other sorbents
<5% fat content with color (carotenoids/chlorophyll content high) 45 mg GCB
Food commodities (citric fruits, vegetables, tree nuts, eggs, dairy products, meat, poultry, edible oils, chocolate, coffee, beverages)OPs (acephate, azinphos-methyl, chlorpyrifos, chlorpyrifos-ethyl, diazinon, dimethoate, disulfoton, demeton-S, demeton-S methyl, ethion, fenamiphos, fenitrothion, fenthion, malathion, methamidophos, methidathion, mevinphos, monocrotophos, omethoate, formothion, parathion, parathion-methyl, phorate, phosalone, phosmet, phosphamidon, propetamphos, terbufos, tetrachlorvinphos, triazophos, trichlorfon, dicrotophos, edifenphos, fosthiazate, isofenphos-methyl, naled, phoxim profenofos, tolclofos-methyl, vamidothion, cadusafos, tribufos, coumaphos, dichlorvos, ethoprophos, isocarbophos, phenoate, quinalphos) PSA [84–107%]; PSA/C18 [83–111%]; PSA/GCB [83–110%] at 10 μg/kg;
carbamates (aldicarb, benfuracarb, carbaryl carbofuran, EPTC, fenobucarb, formetanate HCl, isoprocarb, methiocarb, methomyl, molinate, oxamyl, pirimicarb, propamocarb, thiobencarb, thiocarb) PSA [83–106%]; PSA/C18 [85–111%]; PSA/GCB [87–110%] at 10 μg/kg;
OP and carbamate degradates (sulfones, sulfoxides) and carbamate degrades (3-hydroxycarbofuran, methiocarb) [92–114%] at 10 μg/kg
(A) 10 mL 1% CH3COOH in ACN, 4 g MgSO4, 1 g NaCl, Method A, citrate buffer (1 g Na citrate dehydrate, 0.5 g Na2H citrate sesquihydrate)
(B) LLE with 1% HCOOH in acetone
SPE Oasis HLBMilk (10 mL)Azoles (azaconazole, epoxiconazole, fenbuconazole, paclobutrazol, thiabendazole, triflumizole) [(A) 82 to >130, (B) 35–114%];
carbamates (aldicarb, carbaryl, carbofuran, diethofencarb, iprovalicarb, methiocarb, methomyl, propamocarb, promecarb, thiophanate-methyl) [(A) <30 to >130%; (B) <30–138%];
neonicotinoids (acetamiprid, imidacloprid, thiacloprid) [(A) 67–123% (B) 83–124%];
benzoyl and phenylureas (diflubenzuron, isoproturon, linuron, metobromuron, metoxuron, monolinuron, pencycuron) [(A) 91 to >130%; (B) <30 to >130%];
sulfonyl ureas (chlorsulfuron, cinosulfuron, iodosulfuron methyl, triasulfuron, thifensulfuron methyl (A) <30–107%; (B) [<30–87%];
triazines (atrazine, metribuzin, propazine, sebuthylazine, simazine, terbuthylazine) [(A) 63 to >130%; (B) <30 to >130%].
15 mL ACN with6.0 g MgSO4 and 1.5 g NaClSPE Envicarb (GCB) + SPE Silica (pyrethroids)
SPE C18 (pyrethroid degradates)
15 g (A) lettuce, pepper, onion, carrot, broccoli
(B) Apple, grape, tomato, orange, banana
Pyrethroids (bifenthrin, cyfluthrin, cyhalothrin, cypermethrin, deltamethrin, esfenvalerate, permethrin) [(A) 49–11%; (B) 50–115%];
pyrethroid metabolites (3-PBA, DCCA, 4-F-3-PBA, DBCA, MPA [(A) 73–136%, (B) 61–121%])
10 mL ACN rinse with 1 mL ACN, citrate buffer (4 g MgSO4, 1 g NaCl, 0.5 g Na2Hcitrate-1.5H2O, 1 g Na3citrate dihydrateHigh fat (wheat flour, rolled oats, wheat germ): salt, PSA, C18
Rich in carotene and chlorophyll (red pepper): salt, PSA, GCB
Others (fruits and vegetables) salt, PSA
Wheat flour
and wheat germ shown %
OCs and other halogenated pesticides (aldrin, alachlor, benfluralin, dichlobenil, dieldrin (58–76%), heptachlor, HCHs, heptachlor epoxide, hexachlorobenzene (42–77%), endosulfan, endosulfan sulfate, iprodione, pendimethalin, trifluralin, triallate, vinclozolin) [most > 80–105% exceptions in brackets];
OPs (bromfenvinphos-methyl, bromophos-methyl, chlormephos, chlorpyrifos, coumaphos, diazinon, dichlorvos, heptenophos, ethoprophos, fenchlorphos, fenthion, fenitrothion, isofenphos, isofenphos-methyl, malathion, mevinphos, parathion, parathion-methyl, tolclofos-methyl) [>80%];
pyrethroids (λ-cyhalothrin, cypermethrin, deltamethrin, esfenvalerate, fenvalerate, flucythrinate, permethrin) [72–103%]
EtOAc or ACN, 4 g MgSO4, 1 g NaOAc100 mg PSA, GCB, Zr-Sep+ or C18 or mix of all at 50 mg eachSoya-based nutraceutical–wetted78–92% of pesticides in 70–120% with ethyl acetate; 3–28% with acetonitrileGC-EI-MS/MS[15]
15 mL 1% CH3COOH in ACN, 6 g MgSO4, 1.5 g NaOAc200 mg PSA, 600 mg MgSO4Parsley, lettuce, spinachAzoles (cyproconazole, difenoconazole, epoxiconazole, penconazole, propiconazole, tebuconazole, triadimefon, triadimenol, triflumizole) [90–100%]; carbamates (carbaryl, carbofuran, carbosulfan, ethiofencarb, fenoxycarb, methiocarb, oxamyl, pirimicarb) [78–111%]; OPs (chlorpyrifos, diazinon, dichlorvos, malathion, dimethoate, profenofos, prothiofos) [86–106%]; pyrethroids (bifenthrin, cypermethrin, deltamethrin, tau-fluvalinate) [98–102%]; neonicotinoids (acetamiprid, imidacloprid, kresoxim-methyl, thiamethoxam) [77–91%]; strobilurin fungicides (azoxystrobin, trifloxystrobin) [87–103%].LC-ESI+-MS/MS[16]
15 mL EtOAc, 4 g MgSO4, 1.5 g NaClFreeze-out, 100 mg Al2O3, 60 mg C18, 600 mg MgSO4(5 g) bovine liver and muscleAzoles (tebuconazole, tebufenozide) [73–109%], Benzoylphenylurea (triflumuron) [77–91%]; neonicotinoids (thiacloprid, thiamethoxam) [71–85%]; strobilurin fungicide (trifloxystrobin) [82–94%], other (Spinosyn D) [70–78%]LC-ESI+-MS/MS and GC-EI-MS[17]
ACN, 4 g MgSO4, 1g NaCl, 0.6 g Na2Hcitrate sesquihydrate, 1 g Na3citrate dihydrateFreeze-out followed by dSPE with 25 mg PSA and 150 mg MgSO4Wheat flour (wetted), fruits and vegetablesOrganophosphorus pesticides (chlorpyrifos, chlorpyrifos-methyl, fenitrothion, malathion quinalphos) [wheat flour 99–104%];
pyrethroids (bifenthrin, λ-cyhalothrin) [wheat flour 93–99%];
strobilurin fungicides (azoxystrobin, trifloxystrobin) [wheat flour 103–106%]
Azoles (difenconazole, tebuconazole) [88–96%]; carbamates (aminocarb, fenobucarb, prochloraz, propamocarb, thiobencarb) [73–108%]; neonicotinoids(acetamiprid, clothianidin, imidacloprid, nitenpyram, thiacloprid, thiamethoxam) [wheat flour 76–102%]; phenylureas (diflubenzuron, flufenoxuron, lufenuron, monolinuron) [wheat flour 86–98%]
10 mL ACN, 4 g MgSO4, 1 g NaCl1 g EMR-Lipid, 1.6 g MgSO4, 1 g NaCl10 g olive oil or avocadoAzoles (difenoconazole, paclobutrazol, penconazole, tebuconazole, tetraconazole) [76–116%]; carbamates (carbaryl, carbendazim, carbofuran, methomyl) [77–117%]; OPs (acephate, azinphos-methyl, chlorfenvinphos, chlorpyrifos [45–51%], chlorpyrifos-methyl [66%], diazinon [102–121%], dimethoate, fenamiphos, fenthion, malathion, methamidophos [60–67%], pirimiphos-methyl, quinalphos, trichlorfon) [71–103%]; neonicotinoids (acetamiprid, imidacloprid, kresoxim-methyl, thiacloprid, thiamethoxam) [82–102%]; phenylureas (chlorotoluron, diuron, flufenoxuron, isoproturon) [73–99%]; strobilurin fungicides (azoxystrobin) [92–96%]LC-ESI+-MS/MS[19]
10 mL ACN with 0.68 mL HCOOH, 2.5 g NaCl30 mg PSA, 100 mg C18, 60 mg GCB, 150 mg MgSO4pepperNeonicotinoid (thiacloprid); spirotetramat and its metabolites [100; 76–89%] at 5 μg/kgLC-ESI+-MS/MS[20]
1% CH3COOH in ACN, 2 g MgSO4 + 500 mg NaOAc125 mg PSA and 375 mg MgSO4Bivalve Scrobicularia planaOCs and related halogenated pesticides (alachlor, aldrin, cyhalofop-butyl, DDD, DDE, DDT, endosulfan, endosulfan sulfate, endrin, HCB, heptachlor, heptachlor epoxide, lindane, mirex, methoxychlor, metoachlor, trifluralin) [81–119%]; OPs (azinphos-methyl, chlorpyrifos, diazinon, dichlorvos, dimethoate, fenamiphos, fenitrothion, fonofos, malathion, methamidophos, parathion, parathion-methyl, phosmet, tetrachlorvinphos) [81–110%]
Pyrethroids (cyfluthrin, cyhalothrin, cypermethrin (6%), deltamethrin)[94–114%]; triazines (atrazine, cyanazine, metribuzin, propazine, propyzamide, simazine, terbuthylazine) [85–105%].
10 mL ACN, 4 g MgSO4, 1 g NaCl200 mg MgSO4, 200 mg C18Parsley, basil, mint, thyme, salviaCarbamates (aldicarb, asulam, benfuracarb, benomyl, benthiocarb, carbaryl, carbendazim, diethofencarb, ethiofencarb, fenobucarb, fenoxycarb, isoprocarb, oxamyl, methiocarb, pirimicarb, propamocarb, promecarb, propoxur) [72–98%] at 2 μg/kgLC-ESI+-MS/MS[22]
10 mL ACN150 mg Z-Sep+ and 150 mg MgSO4Edible oils (olive, sunflower, maize, linseed and sesame oils) (3:7 dilution with water)Carbamates (aldicarb, asulam, benomyl, benthiocarb, carbaryl, carbendazim, carbofuran, diethocarb, ethiofencarb, fenobucarb, fenoxycarb, isoprocarb, oxamyl, methomyl, methiocarb, metolcarb, napropamid, pirimicarb, promecarb, propamocarb, propoxur, thiodicarb) [71–104%]LC-ESI+-MS/MS[23]
10 mL ACN, followed by freeze-out (−20°C for fat precipitation)150 mg PSA, 40 mg activated charcoal sorbent, 300 mg MgSO4Edible oils
(rice bran and nut oil)
OCs (aldrin, chlordane, dieldrin, DDD, DDE, DDT, endosulfan, endrin, HCHs, heptachlor) [70–103%];
OPs (dichlorvos, chlorpyrifos, diazinon, fenitrothion, malathion, parathion, parathion methyl, phorate, quinalphos, profenofos, phosmet, phosalone) [67–96%]; Pyrethroids (allethrin, cyfluthrin, cypermethrin, deltamethrin, flumethrin) [68–88%] at 20 ng/g
1% CH3COOH in 10 mL ACN, 4 g MgSO4, 1.7 g NaOAc40 mg PSA, 150 mg MgSO4Orange juiceAzoles (bromuconazole, difenoconazole, epoxiconazole, penconazole, propiconazole, tebuconazole, tebufenozide, tetraconazole, thiabendazole) [89–117%]; carbamates (carbaryl, carbofuran, carboxin, mecarbam, thiobencarb) [81–101%]; neonicotinoids (acetamiprid, thiacloprid) [101–106%]; OPs (diazinon, dicrotophos, dimethoate, ethoprophos, fenamiphos, monocrotophos, o-methoate, triazophos) [82–113%]; Phenylureas (diuron, linuron, monolinuron) [90–101%]; strobilurin fungicides (azoxystrobin, dimoxystrobin, picoxystrobin) [84–112%].LC-ESI+-MS/MS[25]
1% CH3COOH in 10 mL ACN, 4 g MgSO4, 1.7 g CH3COONa100 mg PSA, 500 mg C18, 600 mg MgSO4 per 4 mL extractCoconut water and pulpAzole carbendazim (59% in water), cyproconazole, difenoconazole, thiabendazole, thiophanate-methyl (172% in water) [72–94%]; carbamate (carbofuran) [115 water and 78% pulp]; neonicotinoid (thiamethoxam) [100% water and 96% pulp].LC-ESI+-MS/MS[26]
10 mL ACN with 4 g MgSO4 and 1 g NaCl50 mg PSA, 100 mg C18, 100 mg MgSO4Meats (high proteins and fats)Pyraclostrobin, propiconazole, isopyrazam [76–94%] at 5 μg/kgLC-ESI+ -MS/MS[27]
10 mL ACN with 4 g MgSO4 and 1 g NaCl25 mg PSA +150 mg MgSO4Soil (wetting by diluted 1:1 with H2O)Neonicotinoids (acetamiprid, clothianidin, imidacloprid, thiacloprid, thiamethoxam) [94–105%]LC-ESI+-MS/MS[28]

Table 1.

Modified QuEChERS methods for pesticides.


Figure 1.

QuEChERS and modified QuEChERS approaches.

The selection of dSPE sorbent also depends on the target list of pesticides. The use of GCB can reduce recoveries of some pesticides including planar pesticides such as carbendazim, coumaphos, and other pesticides including prochloraz, boscalid, and pyraclostrobin due to strong absorption onto GCB [14]. The use of 25% toluene solution (v/v) can desorb planar pesticides and improve recoveries. The mass of dSPE sorbent is also optimized with reduction of mass improving recoveries for strobilurin fungicides and neonicotinoids along with other problematic pesticides [3]. The original QuEChERS method used 25 mg PSA per mL of extract, but others have increased PSA to 50 mg per mL of acetonitrile extract to obtain recoveries >77% [8].

The original QuEChERS version included no pH control, while current methods use acetate of citrate buffer for pH control to address pesticides that are partially ionized or those that degrade particularly at basic pH conditions such as observed for captan, folpet, dichlofluanid, and tolylfluanid [25, 26]. The buffers are selected as they allow for buffering to pH 4–5.5 for acid sensitive pesticides with minimal loss of base-sensitive pesticides. Some food commodities such as coconut water and pulp also see reduced co-extracts with use of acetate buffer [26]. Comparison of different QuEChERS including the original (salt only), CEN EN 15662 Standard Method (citrate buffer), and AOAC method (acetate buffer) show that recoveries ≥80% can be obtained for all methods for most pesticides (chemical classes including azoles, carbamates, organophosphorus pesticides, and strobilurin fungicide) in fruit and vegetable matrices analyzed by both GC and LC-MS/MS methods [32]. Acetate buffer pH 4.8 and citrate buffer 5.0–5.5 are used for low pH susceptible compounds such as thiabendazole and imazalil [25]. Low pH samples such as orange juice (pH~3.5) also need pH adjustment during extraction to efficiently extract pesticides of a range of polarities [25]. C18 cleanup decreased the differences in recoveries of the acetate and citrate buffer QuEChERS approaches and was generally found to further improve recoveries [32]. Target analytes with the lowest recoveries included folpet (63–69%) and tolylfluanid (63–71%) analyzed by GC and pymetrozine (31–82%) and tolylfluanid (60–76%) analyzed by LC methods [32]. Ethyl acetate instead of acetonitrile (extraction solvent) has also been used particularly for GC-amenable pesticides [15, 17, 31, 33], but the dSPE is generally more effective with acetonitrile and in some matrices such as peas, the co-extractives may increase significantly when ethyl acetate is used as the extraction solvent [32]. Others have found that the number of GC-amenable pesticides increases with the use of ethyl acetate rather than acetonitrile and good recoveries were obtained with dSPE using a mixture of PSA, GCB, PSA, and Zr-Sep+ [15]. Recoveries improved for cleanup of extracts for analysis of OPs and carbamates by LC-MS/MS (egg products) when acetonitrile, rather than ethyl acetate, was used, and when ethyl acetate was used, recoveries >120% were reported even when followed by dSPE [31]. The use of freezing-out after ethyl acetate salt-out extraction can remove the high lipid content in the co-extracted matrix and if this is followed by C18 and Al2O3 addition for removal of lipophilic compounds, fatty acids, sugars, and other acidic compounds (along with MgSO4 to handle water content and high protein content of extracts) it provides better recoveries than when only dSPE with PSA, C18, and Al2O3 combinations was used [17]. Buffering of the ethyl acetate extraction can also improve recoveries particularly when the sample matrix is acidic, but care should be taken to minimize ionization of the acidic pesticides (which subsequently increases their solubility in the aqueous phase) [33].

Acetone is a poor extraction solvent and has been found to poorly recover polar analytes such as acephate and cyromazine [31]. Addition of hexane to acetonitrile prior to salt-out has been used to improve recoveries of OPs for bee samples that contain co-extracted beeswax with exception of diazinon and coumaphos that observed a drop in recoveries of 22 and 12%, respectively [5]. Recoveries of neonicotinoids from pollen also improved with addition of hexane to acetonitrile due to the high wax content [7]. Chloroform has also been added to acetonitrile to reduce the amount of acetonitrile remaining in the aqueous phase after phase separations and to further improve the partitioning of polar OPs (methamidophos and acephate) into acetonitrile [29].

For food commodities, the recoveries of analytes analyzed by LC-MS/MS (OPs, azoles, sulfonylureas) increased with dSPE following the salt-out acetonitrile extraction, while for analytes (OCs, OPs, pyrethroids) analyzed by GC-MS/MS, recoveries often decrease into an acceptable range of 70–120% [30]. PSA can bind some analytes strongly such as cinosulfuron that observed 20% decrease in recoveries [30]. Some OPs may exhibit better recoveries with GC-MS/MS rather than LC-MS/MS methods as observed for acephate and methidathion [30]. GC-amenable pesticides tend to include the more lipophilic pesticides, particularly OCs and pyrethroids that have a higher tendency to be extracted with the fatty acid matrix components. If the sample has a low water content, a wetting step is often used; however, if the sample matrix has a high fat content such as wheat flour (5 mg/mL extract) and wheat germ (45 mg/mL) then removing this wetting step (using the Ultra Turrax) will avoid the potential for target analytes such as OCs and pyrethroids to partition into the fatty layer that can form when water is present [14]. QuEChERS method has also been used with a freeze-out step prior to dSPE with PSA for sample matrices with higher levels of co-extracts including lipids (or waxes and sugars) such as wheat flour and citrus extracts [18]. This step can minimize the need for use of other dSPE sorbents. PSA with C18 has improved the recoveries of neonicotinoids from pollen and high fructose corn syrup when the sample is diluted in water (1:4 or 1:8) prior to extraction with acetonitrile (neonicotinoids would be protonated under acidic conditions such that buffers are not used during the salt-out extraction) [7]. Extracts from soil samples also had better recoveries for neonicotinoids when extracted without buffering of acetonitrile (along with salt-out with MgSO4 and NaCl) [28]. C18 (200 mg) alone was used for extract cleanup for analysis of carbamates by LC-MS/MS and found to be better than other dSPE sorbents [22]. The addition of 200 mg of MgSO4 was also used to improve the removal of water so that the evaporation of organic solvent was quicker. QuEChERS with acetate buffer observed low recoveries for PSA + C18 when larger amounts of sorbent were used such that it is often preferred to use only 50 mg C18 [27]. PSA without C18 or GCB was found to provide better recoveries and precision for neonicotinoids in soil [28]. C18 can result in poor recoveries of some more nonpolar GC-amenable analytes (recoveries <70 or >120%) when the sample matrix has a high fat content and, under these situations, Zr-Sep+ has been used to remove lipids [15]. Zr-Sep+ was also used for cleanup of extracts from high fat content edible oil samples reducing matrix effects better than observed with PSA and C18 [23]. Activated charcoal with PSA has also been used for edible oils [24]. A new material called enhanced matrix removal (EMR)-Lipid was also found to perform similar or better than Zr-Sep+ or PSA+C18 for high fat content vegetable matrices with good recoveries for azoles, OPs, neonicotinoids, and phenylureas [19].

The addition of protectants including 3-ethoxy-1,2-propanediol and D-sorbitol prior to GC-MS/MS analysis can also minimize strong interactions of target analytes and matrix with the injector liner or GC column [14, 15, 21]. Re-acidifying extracts after cleanup with acetic or formic acid have also been used to improve peak shapes and response for GC-MS or LC-MS/MS methods and protect analytes that are sensitive to degradation at high pH [18].

QuEChERS approach does not always provide adequate recoveries at low concentrations and issues with large matrix peaks can still be observed in some separations of difficult matrix samples. Consequently, QuEChERS method has been modified to use cartridge SPE cleanup rather than dispersive SPE (Figure 1) [12, 13, 34]. Recoveries of pyrethroids and their metabolites improved with the use of cartridge SPE rather than dSPE with 42% of recoveries ≥90%, 70% were ≥80%, 90% were ≥70%, although a range in recoveries was still observed [13]. Metabolites 3-PBA and 4-F-3-PBA did not elute from GCB such that C18 SPE was selected and for some food commodity matrices, a second SPE step with silica or C18 was required [13]. A tandem GCB and PSA cartridge has been used for the cleanup of soil extracts after salt-out acetonitrile extraction for the analysis of range of pesticide classes including azoles, Ops, and pyrethroids [34]. For a wide range of chemical classes of varying polarity, Oasis® HLB (hydrophilic liquid balance) (SPE) was used after the acetonitrile with citrate buffer salt-out extraction to remove additional co-extract matrix components [12]. Although C18 can also provide good recoveries, it is more prone to clogging problems from turbid extracts (in food matrices extract may contain lipids and proteins) such that Oasis HLB is often preferred (Table 2) [12].

SPE sorbent (mg)Elution solvent (volume mL)Sample typePesticide chemical classes [average recoveries %]Analysis methodReference
C18 SEP-PAK (500)DCM (5)Urine (diluted 1:1 with H2O)Azoles, OCs, OPs, selected neonicotinoids (kresoxim methyl), pyrethroids [62–109%]
Azoles, carbamates, neonicotinoids, phenylureas, strobilurin fungicides [61–101%]
C18 Empore extraction disksACN (20)WaterOP (temephos and its degradation products)LC-ESI+-MS[37]
C18 (200)ACN (5.5)WaterCarbamates [90–99%]LC-ESI+-MS[38]
C18, top,+ aminopropyl, bottomNot specifiedDust (ultrasonic ext with methylene chloride)Pyrethroids and metabolites [51–101%, resmethrin 23%]GC-EI-MS/MS[39]
C18 (500)MeOH (3)UrinePyrethroid metabolites [90–98%]GC-EI-MS[40]
C18 (500)EtOAc (5)Air sorbents (filters, polyurethane foam, XAD-2, Tenax-TA), PSE EtOAcOCs and OPs [80–110%]GC-NCI-MS[41]
C18 (500) followed by DLLMEMeOH (1.5)WaterOCs, OPs, pyrethroids, selected carbamates (carbaryl, pirimicarb) [79–94%]GC-EI-MS[42]
ProElut C18 (200)DCM:MeOH (9:1)Blood serumOPs [90–118%] 2.7 ng/mLGC-EI-MS/MS[43]
OMICs C18 TIP, μSPEACN (0.05)Wheat (ACN pH 5 ext.)OPsLC-ESI+-MS/MS[44]
Activated carbon μSPE, (100)EtOAc (2.5)Vegetables and fruits (microwave ext. with hexane)OPs [92–105%]GC-EI-MS[45]
CleanInert TPT (three materials) (remove pigments, alkaloids, polyphenols)ACN:toluene 3:1 (20)teaTeaCarbamates, OCs, OPs, pyrethroids and selected others [88–101%] 5 μg/kgGC-EI-MS/MS[46]
GPC + FlorisilHexane:DCM 5:95 (8)MilkOCsGC-EI-MS[47]
Sep-Pak C18 (500)MeOH (10)WaterAzoles [92–122%], carbamates [OPs [0–108%], strobilurin fungicide [60%], triazine [123–127%] 20 ng/mLLC-ESI+-MS/MS[48]
GCB (300)MeOH (1) + DCM:MeOH 80:20 (5)Water (pH 2)Carbamates [83–100%], OPs [78–97%], phenylureas [91–99%], sulfonylureas [90–102%] Protocol 2LC-ESI+-MS/MS[49]
Oasis HLB (150)MeOH or ethanol (4)Tap water (pH 3)Chlorinated pesticides (alachlor, pentachlorophenol), OP (chlorfenvinphos), triazine (atrazine, simazine), phenylurea (isoproturon) [>80%]LC-ESI+-MS/MS[50]
Oasis HLB (150)MeOH (5), ACN (5)Water (tap, surface, etc.)OCs (metolachlor, metazachlor) [76–88%], phenylureas (isoproturon, chlorotoluron, diuron) [86–91%], triazines (atrazine, deethylatrazine, simazine, terbuthylazine) [77–85%]LC-ESI+-MS/MS[51]
Oasis HLB (200)EtOAc (6)WaterOCs [85–116%], OPs [91–112%], pyrethroids [92–113%], triazines [92–112%]GC-EI-MS and GC-EI-MS/MS[52]
Oasis HLB (60)DCM (1) + MeOH (1)Water pH 2.5OCs [55–91%], OPs [35–102%], pyrethroids [74–92%]
Azoles [7891], carbamates [86–90%], strobilurin fungicides [77–92%], phenylureas [88–98%]
Envir-carb+NH2-LCACN:toluene 3:1 (25)Berries (ACN salt-out ext.)OCs, OPs, selected azoles, and other GC-amenable pesticidesGC-EI-MS[54]
Oasis HLB (60) or Strata®-X (200)MeOH (1)Water (NH4Ac addition prior to SPE)Neonicotinoids [85–104%]LC-ESI+-MS/MS[55]
Oasis HLB (500)ACN (5)ChesnutChestnut, shallot, ginger diluted with water (LLE with ACN)Neonicotinoids [82–95%] at 0.01 mg/kgLC-ESI+-MS/MS[56]
C18 (1000)MeOH (5)Atmospheric particles collected on filtersNeonicotinoids and strobilurin fungicides [92–101%]LC-ESI+-MS/MS[57]
dSPE: SBA-15-NH2 (polyphenols removal)ACN:MeOH 7:3teaTeaNeonicotinoids [73–85%]LC-ESI+-MS/MS[58]
Florisil (500)MeOH (5)Honey (1 g diluted 3 mL water:MeOH)Neonicotinoid (thiamethoxam)+fipronil and degradation products [90–102%]LC-ESI (+or ) –MS/MS[59]
Oasis HLB (225)MeOH (5)Apple-based infant foods (LLE with ACN)Carbamates and degradates, azole (thiabendazole) [71–95%]LC-ESI+-MS/MS[60]
Oasis HLB (10)ACN (1)Rice powder (microwave ext – aqueous extract)Carbamates (aldicarb, carbaryl, carbofuran, isoprocarb, methomyl, metolcarb, propoxur), phenylurea (diuron) [67–103%] at 10 ng/gLC-ESI+-MS/MS[61]
Zorbax C18 (500)MeOH:ACN 1:1 (3)WaterCarbamates [74–93%]LC-ESI+-MS[62]
Graphene (30)Acetone (5)WaterCarbamates [55–95%]LC-ESI+-MS/MS[63]
Graphene (50)EtOAc (20)Apple juiceOPs [94–105%]LC-ESI+-MS/MS[64]
C18 (1000)EtOAc (5)Air sorbents XAD-2, Tenax-TA, polyurethane foam, PSE EtOAcOPs, Opoxons, and other OP degradation products [70–100%]LC-ESI+-MS/MS[65]
C18 (1000)0.1% HCOOH in EtOAc-2-Propanol-ACN, 10:55:35, (0.425)Air sorbents XAD-2, Tenax-TA, polyurethane foam, PSE EtOAcAzole fungicides [80–108%]LC-ESI+-MS/MS[66]
CN-SPE (500)DCM:MeOH 98:2 v:vPotato, tomato, orange (LLE)Carbamate (aldicarb and aldicarb sulfone and sulfoxide) [68–89%]LC-APCI+-MS[67]
Oasis HLB (60)MeOH (3)wastewaterWastewaterMetabolites of triazines, OPs, pyrethroidsLC-ESI (+ or )-MS/MS[68]
Bond Elut SAX + Strata-XNot specifiedMeconium samples from babiesCarbamate (propoxur), OPs, OP metabolites (dialkylphosphates), pyrethroids and metabolites, triazolesLC-ESI (+ or )-MS/MS[69]
Strata X-AWParent pesticides EtOAc (5); degradates MeOH:HCOOH 90:10 v/v (3)Meconium samples from babiesOPs (chlorpyrifos, diazinon, malathion), OP degradates, pyrethroids and degradate, carbamates, phenylurea and metabolite, phenoxyacid herbicideLC-ESI (+ or )-MS/MS[70]
Oasis HLB 96 well plate format (30)Acetone (0.75)UrineMetabolites of OPs [51–92%] and pyrethroids [86–97%]LC-ESI (+ or )-MS/MS[71]
Silica SPE (1000)
1.ISOLUTE ENV+ (200)
2.Bond Elut PPL (200)
MeOH (10)
1. DCM/EtOac 1:1 v:v (6)
2. DCM/EtOAc 1:1 v:v (6)
Urine LLE EtOAc
Urine diluted with NH4Ac buffer (25:10)
Oxy-pyrimidine metabolites of diazinon [LLE +SPE 95:106; SPE only 83–114%]GC-EI/MS
Carbograph (100)Toluene (8)HoneyPyrethroid (tau-fluvalinate), OP (coumaphos), Others: amitraz, fipronil, bromopropylate [99–106%]GC-PTV-EI-MS[73]
Oasis HLB (200)MeOH (8)wastewaterWastewaterDiazinon, IMP, pharmaceuticalsLC-ESI+-QTOF/MS[74]
Sep-Pak Plus PS -2, C18 (665) or
Oasis HLB (225)
ACN (5), followed by EtOAc (3)Surface waterOPs, triazines, and selected others [76–99%]LC-ESI+-MS/MS[75]
Oasis HLB (200)MeOH (5) followed by EtOAc (5)Surface waterOCs [45–101%], pyrethroids [45–91%]
Azoles [84–133%], carbamates [84–140%], neonicotinoids [104–119%], OPs [68–102], triazines [95–164%]
Bond Elut Nexus (polymeric)MeOH + DCM (1)Water and wastewater (acidified pH 3)OCs and OPs [70–120%] some selected OPs and OCs outside of rangeGC-EI-MS/MS[77]

Table 2.

Solid phase extraction (SPE) methods for pesticides.

For some basic analytes, such as pymetrozine which is highly polar, QuEChERS gives poor recoveries as the analyte remains in the aqueous phase as a protonated molecule and adjusting the pH of the extraction leads to problems with recoveries of other acidic or basic analytes. Liquid extraction with acetonitrile (without phase separation using salt) can provide better recoveries than QuEChERS for these analytes, as it does not discriminate basic analytes [35].

3. Solid phase extraction for preconcentration or extract cleanup

Figure 2 illustrates the application of solid phase extraction for different sample matrix types. Solid phase extraction is widely used for the preparation of liquid sample matrices including food beverages, biological fluids, and water samples (drinking water, surface water, ground water). It is also widely used as a cleanup step following prior extraction steps for solid samples such as bee products, air sampling sorbent materials, and soil samples (Table 2) [3677]. For solid sample matrices, popular initial extraction approaches include pressurized liquid extraction (PLE), microwave extraction (MAE), ultrasonic extraction, or liquid-solid extraction [41, 45, 57, 61, 66, 7896]. Often an organic solvent is selected for the initial extraction of pesticides from the solid materials such that the SPE procedure must be adapted to accommodate the organic content of the sample extract to ensure adequate sorption of target analytes or the sample is diluted with water if feasible prior to SPE.


Figure 2.

Strategies for extraction utilizing solid phase extract for preconcentration or extract cleanup.

Table 2 shows common SPE sorbents used along with sample matrix type and target chemical classes of pesticides. SPE sorbents include bonded silica phases such as C18 (or less commonly selected C8); polymeric phases with an aromatic moiety to give stronger retention for more aromatic pesticides through π-interactions; Oasis HLB which is made of a copolymer consisting of divinylbenzene and N-vinylpyrrolidone; carbon based sorbents including graphene for removal of pigments; and NH2-based sorbents for removal of polar matrix components such as sugars and proteins. N-vinylpyrrolidone acts as a hydrophilic group to give the Oasis HLB sorbent a mixed mode of retention and can improve the retention of more polar pesticides that are weakly retained on C18 sorbents. New generation molecularly imprinted polymers have also been used for cleanup of extracts for analysis of OCs [97]. Both the retention of target analytes and matrix co-extracts must be considered when optimizing an SPE procedure with sample pH and volume during loading, type of SPE sorbent, and extraction solvent and volume optimized. Conditioning solvents for the SPE sorbents are also an important consideration, particularly for liquid extracts that contain an organic solvent from a prior extraction step.

C18 sorbents are more effective at retaining nonpolar pesticides than Oasis HLB with solvent used for extraction often more nonpolar to improve solubility of the target analytes [36]. Selection of elution solvent should also consider a need to dry or evaporate the solvent after SPE either as a preconcentration step or for solvent exchange compatibility for GC-MS or LC-MS/MS analysis. Nonpolar solvents often have higher volatility with ethyl acetate, dichloromethane or mixtures of dichloromethane with methanol commonly selected [36].

For a range of polarity of pesticides (covering both GC- and LC-amenable pesticides), dichloromethane was better at recovering more pesticides (70 and 90 pesticides) compared to methanol (10 and 30 pesticides for LC- and GC-amenable pesticides) [36]. For triazines and phenylureas at acidic sample, pH recoveries were better with Oasis HLB compared to polymeric sorbents (two different nonfunctionalized styrene divinylbenzene (SDVB), hydroxylated SDVB) [51]. Oasis HLB and Strata-X gave good recoveries of neonicotinoids with a lower sample water volume and sorbent amount (60 mg) for Oasis HLB allowing for a small solvent elution volume (1 mL), thereby removing the need for a drying step [55]. Dinotefuran (most polar) and thiacloprid (least polar) had low recoveries due to matrix effects with recoveries improving to 60% with Oasis HLB with a washing step with 5% methanol [55]. For carbamates, better recoveries were observed with Oasis HLB when acetonitrile rather than methanol or ethanol was used as the elution solvent (the lowest recovery observed for methomyl with all solvents) [61]. Oasis HLB, Strata-X, and Strata-C18 were also shown to provide recoveries between 70 and 120% for more pesticides when water samples were acidified to pH 2.5 for both GC- and LC-amenable pesticide classes [53]. Under the optimized method, more pesticides had acceptable recoveries with Oasis HLB as expected from this mixed-mode sorbent [53]; however, recoveries for OCs and OPs varied (Table 2), so care should be taken if more nonpolar pesticides are of greatest interest. Under neutral pH conditions, recoveries for OCs and pyrethroids were also more variable when Oasis HLB was used [76]. A larger number of OPs and OCs gave acceptable recoveries with a polymeric sorbent with acidified water samples (Bond Elut Nexus) [77]. Chlorpyrifos and pendimethalin observed low recoveries with Sep-Pak plus PS-2 (C-18) with 5 mL acetonitrile as an elution solvent, but recoveries were improved to >76% with a second elution with 3 mL of ethyl acetate [75]. Carbamates gave good recoveries with C18, while other sorbents including Oasis HLB and carbon-graphitized cartridges gave good recoveries for these carbamates except for pirimicarb and carbofuran [62]. Hydroxylated polystyrene-divinylbenzene copolymer also gave poor recoveries for pirimicarb. Poor recoveries were observed for acephate, chlorpyrifos, and methamidophos in water with C18 SPE, although other OPs observed acceptable recoveries [48]. Graphene is a new SPE sorbent and performs slightly better than C18 or GCB for carbamates except for carbaryl which has lowest recovery of ~55% attributed to stronger π-π interactions with graphene than other sorbents [98]. PRS performed the worst for carbamates of all sorbents tested [98]. Carbon-based sorbents are often selected to remove pigments with the elution solvent selected as toluene or toluene:acetonitrile rather than dichloromethane or ethyl acetate as nonpolar analytes that can bind more strongly to this sorbent material [73, 54]. For extraction of OPs with graphene, ethyl acetate was found to provide better recoveries than dichloromethane or acetonitrile as the elution solvent [99]. Graphene sheets with covalently bonded Fe3O4 have also been used for magnetic solid phase extraction (MSPE) of organochlorines in orange juice [100]. Other modified MSPE with Fe3O4 including coated carbon nanotubes has been utilized for water or fruit juice extraction of GC-amenable pesticides [99, 101]. Zirconia nanoparticle-decorated calcium alginate hydrogel fibers have been used for extraction of OPs from water and fruit juices [102].

For added selectivity, a molecularly imprinted polymer has been used for SPE sorbent for the analysis of OCs in water, soil, rice, and tea leaves [97]. Micro-SPE has also been used in combination or after extraction methods for recovery of OPs [4445]. On-line SPE coupled with LC-MS/MS has been used with many of the same sorbents materials described for off-line methods with C18 or C8 and PLRP-s (styrene divinyl-benzene copolymer sorbent) as popular choices [103106].

4. Other considerations

Solid matrices including soil, sediment, food commodities, and air sampling solid sorbent materials (filter, polyurethane foam, solid sorbents (XAD-2, XAD-4, Tenax-TA)) are extracted prior to an SPE (or dSPE) cleanup step with a variety of approaches including microwave extraction, pressurized liquid extraction (as referred commonly as pressurized solvent extraction), ultrasonic extraction, and traditional solid-liquid extractions [41, 45, 57, 61, 66, 7896]. These approaches are not selective and the polarity of the organic solvents and choices of additives in these extraction procedures will impact the co-extractive matrix, which necessitate the subsequent SPE or dSPE cleanup choices. In addition, for SPE, aqueous extracts are easier to optimize SPE loading, washing, and elution steps as extracts of organic solvents need careful consideration to ensure adequate retention of target analytes on sorbent materials to prevent washout. The most common solvent choices for pressurized liquid extraction and microwave extraction of solid matrices were acetonitrile, ethyl acetate, acetone, hexane, or combinations of these solvents [8196]. With microwave extraction, acetone has been added to hexane (2:1) to improve the recoveries for polar OPs, while use of hexane can reduce matrix co-extractives [81]. Ethyl acetate and acetone have been used for microwave extraction of azoles [82]. Reduction in co-extracts has also been reported with acetonitrile rather than methanol or acetone (with microwave extraction) and good recoveries have been reported for OCs and neonicotinoids [83, 84]. Hexane, dichloromethane, ethyl acetate, acetone, and acetonitrile have also been commonly used for pressurized solvent extraction of a large range of polarity of pesticides [41, 57, 65, 66, 8596].


This work was financially supported by the Natural Sciences and Engineering Research Council (NSERC) of Canada.





Atmospheric pressure chemical ionization


Dispersive solid phase extraction


Electron ionization


Enhanced matrix removal-lipid


Electrospray ionization


Ethyl acetate


Graphitized carbon black


Gas chromatography-mass spectrometry


Gas chromatography-tandem mass spectrometry


Liquid chromatography-tandem mass spectrometry


Magnetic solid phase extraction


Microwave assisted extraction


Anhydrous magnesium sulfate




Organophosphorus pesticides


Pressurized liquid extraction


Primary secondary amine


Quick, easy, cheap, effective, rugged, safe


Solid phase extraction


1 - Raina-Fulton R, Dunn N, Xie Z. Pesticides and their degradation products including metabolites: Chromatography-Mass Spectrometry, Methods in Mass Spectrometry. Intech, Rijeka, Croatia, 2017
2 - Raina-Fulton R. Journal of AOAC International. 2015;98:1163‐1170
3 - David A, Botais C, Abdul-Sada A, Goulson D, Hill EM. Analytical and Bioanalytical Chemistry. 2015;407:8151‐8162
4 - Calatayud-Vernich P, Calatayud F, Simo E, Suarez-Varela MM, Pico Y. Science of the Total Environment. 2016;541:33‐41
5 - Barganska Z, Slebioba M, Namiesnik J. Molecule. 2014;19:2911‐2929
6 - Herrera Lopez S, Lozano A, Sosa A, Dolores Hernando M, Fernandez-Alba AR. Chemosphere. 2016;163:44‐53
7 - Chen M, Collins EM, Tao L, Lu C. Analytical and Bioanalytical Chemistry. 2013;405:9251‐9264
8 - Golge O, Kabak B. Food Chemistry. 2015;176:319‐332
9 - Fan S, Zhang F, Deng K, Yu C, Liu S, Zhao P, Pan C. Journal of Agricultural and Food Chemistry. 2013;31:2039–2044
10 - Liu M, Hashi Y, Song Y, Lin J. Journal of Chromatography A. 2005;1097:183‐187
11 - Chung SWC, Chan BTP. Journal of Chromatography A. 2010;1217:4815‐4825
12 - Aguilera-Luiz MM, Plaza-Bolanos PP, Romero-Gonzalez R, Martinez Vidal JL, Garrido Frenich A. Analytical Biochemistry. 2011;399:2863‐2875
13 - Li W, Morgan MK, Graham SE, Starr JM. Talanta. 2016;151:42‐50
14 - Rasche C, Fournes B, Dirks U, Speer K. Journal of Chromatography A. 2015;1403:21‐31
15 - Palenikova A, Martinez-Dominguez G, Arrebelo FJ, Romero-Gonzalez R, Hrouzkova S, Frenich AG. Food Chemistry. 2015;173:796‐807
16 - Esturk O, Yakar Y, Ayhan Z. Journal of Food Science and Technology. 2014;51:458‐466
17 - Souza R, Pareja L, Cesio MV, Heinzen H. Chromatographia. 2016;79:1101‐1112
18 - Paya P, Anastassiades M, Mack D, Sigalova I, Tasdelem B, Oliva J, Barba A. Analytical and Bioanalytical Chemistry. 2007;389:1697‐1714
19 - Lopez-Blanco R, Nortez-Mendez R, Robles-Molina J, Moreno-Gonzalez D, Gilbert-Lopez B, Garcia-Reyes JF, Molina-Diaz A. Journal of Chromatography A. 2016;1456:89‐104
20 - Li S, Liu X, Dong F, Xu J, Xu H, Hu M, Zheng Y. Food Chemistry. 2016;192:893‐899
21 - Cruzeiro C, Rodrigues-Oliveira N, Velhote S, Pardal MA, Rocha E, Rocha MJ. Analytical and Bioanalytical Chemistry. 2016;408:3681‐3698
22 - Natia EA, Moreno-Gonzalez D, Manfo FPT, Gamiz-Gracia L, Garcia-Campana AM. Food Chemistry. 2017;216:334‐341
23 - Moreno-Gonzalez D, Huertas-Perez JF, Garcia-Campana AM. Talanta. 2014;128:299‐304
24 - Deme P, Azmeera T, Devi BLAP, Jonnalagadda PR, Prasad RBN, Sarathi UVRV. Food Chemistry. 2014;142:144‐131
25 - Rizzetti TM, Kemmerich M, Martins ML, Prestes OD, Adaime MB, Zanella R. Food Chemistry. 2016;196:25‐33
26 - Ferreira JA, Ferreira JMS, Talamini V, Facco JF, Rizzetti TM, Prestes OD, Adaime MB, Zanella R, Bottoli CBG. Food Chemistry. 2016;213:616‐624
27 - Mu Z, Feng X, Zhang Y, Zhang H. Analytical and Bioanalytical Chemistry. 2016;408:1515‐1522
28 - Dankyi E, Gordon C, Carboo D, Fomsgaard IS. Science of the Total Environment. 2014;499:276‐283
29 - Liu G, Rong L, Guo B, Zhang M, Li S, Wu Q, Chen J, Chen B, Yao S. Journal of Chromatography A. 2011;1211:1429‐1436
30 - Zainudin BH, Salleh S, Mohamed R, Yap KC, Muhamad H. Food Chemistry. 2015;172:585‐595
31 - Choi S, Kim S, Shin JY, Kim M, Kim J. Food Chemistry. 2015;173:1236‐1242
32 - Lehotay SJ, Son KA, Kwon H, Koesukwiwat U, Fu W, Mastovska K, Hoh E, Leepipatpiboon N. Journal of Chromatography A. 2010;1217:2548‐2560
33 - Jadhav MR, Oulkar DP, Shabeer A, Banerjee K. Journal of Agricultural and Food Chemistry. 2015;63:4449‐4456
34 - Hayward DG, Wong JW, Shi F, Zhang F, Zhang K, Lee NS, DiBenedetto AL, Hengel MJ. Analytical Chemistry. 2013;85:4686‐4693
35 - Lacina O, Zachariasova M, Urbanova J, Vaclavikova M, Cajka T, Hajslova J. Journal of Chromatography A. 2012;1262:8‐18
36 - Cazorla-Reyes R, Fernandez-Moreno JL, Romero-Gonzalez R, Garrido-Frenich A, Martinez Vidal JL. Talanta. 2011;85:183‐196
37 - Lacorte S, Ehresmann N, Barcelo D. Environmental Science & Technology. 1996;30:917‐923
38 - El Atrache LL, Sghaier RB, Kefi BB, Haldys V, Dachraoui M, Tortajada J. Talanta. 2013;117:392‐398
39 - Starr J, Graham S, Stout II D, Andrews K, Nishioka M. Environmental Research 2008;108:271‐279
40 - Angerer J, Ritter A. Journal of Chromatography B. 1997;695:217‐226
41 - Raina R, Hall P. Analytical Chemistry Insights. 2008;3:111‐125
42 - Shamsipur M, Yazdanfar N, Ghambarian M. Food Chemistry. 2016;204:289‐297
43 - Chang C, Luo J, Chen M, Wu K, dong T, He X, Zhou K, Wang L, Chen D, Zhou Z, Wang X, Xia Y. Analytical Methods. 2016;8:4487‐4496
44 - Pelle FD, Di Crescenzo MC, Sergi M, Montesano C, Di Ottavio F, Scarpone R, Scortichini G, Compagnone D. Food Additives & Contaminants, Part A. 2016;33:291‐299
45 - Wang Z, Zhao X, Xu X, Wu L, Su R, Zhao Y, Jiang C, Zhang H, Ma Q, Lu C, Dong D. Analytica Chimica Acta. 2013;760:60‐68
46 - Zhao C, Ding R, Huo L, Li H, Dong Z, Wang F, Yang G, Lu X, Aboul-Enein HY. Journal of AOAC International. 2014;97:1001‐1006
47 - Zheng G, Han C, Liu Y, Wang J, Zhu M, Wang C, Shen Y. Journal of Dairy Science. 2014;97:6016‐6026
48 - Rocha AA, Monteiro SH, Andrade GCRM, Vilca FZ, Tornisielo VL. Journal of the Brazilian Chemical Society. 2015;26:2269‐2278
49 - Yang XJ, Du Z, Lin A, Yuan Q, Wan P, Wong C. Analytical Methods. 2013;5:2083‐2092
50 - Barbosa MO, Ribeiro AR, Pereira MFR, Silva AM. Analytical and Bioanalytical. 2016;408:8355‐8367
51 - Valls-Cantenys C, Scheurer M, Iglesias M, Sacher F, Brauch H, Salvado V. Analytical and Bioanalytical, 2016;408:6189‐6200
52 - Cruzeiro C, Pardal MA, Rocha E, Rocha MJ. Environmental Monitoring and Assessment. 2015;187:669, 21
53 - Donato FF, Martins ML, Munaretto JS, Prestes OD, Adaime MB, Zanella R. Journal of the Brazilian Chemical Society. 2015;26:2077‐2087
54 - Yang X, Zhang H, Liu Y, Wang J, Zhang YC, Dong AJ, Zhao HT, Sun CH, Cui J. Food Chemistry. 2011;127:855‐865
55 - Hao C, Morse D, Zhao X, Sui L. Rapid Communications in Mass Spectrometry. 2015;2225‐2232
56 - Xie W, Han C, Qian Y, Ding H, Chen X, Xi J. Journal of Chromatography A. 2011;1218:4426‐4433
57 - Raina-Fulton R. Journal of Agricultural and Food Chemistry. 2015;63:5152‐5162
58 - Zhang M, Chen H, Zhu L, Wang C, Ma G, Liu X. Journal of Separation Science. 2016;39:910‐917
59 - Garcia-Chao M, Agruna MJ, Calvete GF, Sakkas V, Llompart M, Dagnac T. Analytica Chimica Acta. 2010;672:107‐113
60 - Wang J, Cheung W, Grant D. Journal of Agricultural and Food Chemistry. 2005;53:528‐537
61 - Song W, Zhang Y, Li G, Chen H, Wang H, Zhao Q, He Q, Zhao C, Ding L. Food Chemistry. 2014;143:192‐198
62 - Nogueira JMF, Sandra T, Sandra P. Journal of Chromatography A. 2003;996:133‐140
63 - Shi Z, Hu J, Li Q, Zhang S, Liang Y, Zhang H. Journal of Chromatography A. 2014;1355:219‐227
64 - Han Q, Wang Z, Xia J, Zhang X, Wang X, Ding M. Journal of Separation Science. 2014;37:99‐105
65 - Raina R, Sun L. Journal of Environmental Science and Health, Part B. 2008;43:323‐332
66 - Raina R, Smith E. Journal of AOAC International. 2012;95:1350‐1356
67 - Nunes GS, Alonso RM, Ribeiro ML, Barcelo D. Journal of Chromatography A. 2000;888: 113‐120
68 - Rousis NI, Zuccato E, Castiglioni S. Science of the Total Environment. 2016;571:1349‐1357
69 - Meyer-Monath M, Chatellier C, Cabooter D, Rouget F, Morel I, Lestremau F. Talanta. 2015;138:231‐239
70 - Berton T, Mayhoub F, Chardon K, Duca R, Lestremau F, Bach V, Tack K. Environmental Research. 2014;132:311‐320
71 - Davis MD, Wade EL, Restrepo PR, Roman-Esteva W, Bravo R, Kuklenyik P, Calafat AM. Journal of Chromatography B. 2013;929:18‐26
72 - Yokley RA, Shen N, Cheung MW. Journal of AOAC International. 2000;83:1229‐1238
73 - Notardonato I, Avino P, Cinelli G, Vincenzo-Russo M. Food Analytical Methods. 2016;9:1675‐1685
74 - Gomez-Ramos M, Perez-Parada A, Garcia-Reyes JF, Fernandez-Alba AR, Aguera A. Journal of Chromatography A. 2011;1218:8002‐8012
75 - Shinomiya M. International Journal of Environmental Analytical Chemistry. 2013;93:858‐871
76 - Charalampous AC, Miliadis GE, Koupparis MA. International Journal of Environmental Analytical Chemistry. 2015;1283‐1298
77 - Martins ML, Danato FF, Prestes OD, Adaime MB, Zanella R. Analytical and Bioanalytical Chemistry. 2013;405:7697‐7709
78 - Mohammadi M, Tavakoli H, Abdollahzadeh Y, Khosravi A, Torkaman R, Mashayekhi A. RSC Advances. 2015;5:75174‐75181
79 - Ahmadi K, Abdollahzadeh Y, Asadollahzadeh M, Hemmati A, Tavakoli H, Torkaman R. Talanta. 2015;137:167‐173
80 - Moreno-Gonzalez D, Huertas-Perez J, Garcia-Campana AM, Bosqu-Sendra JM, Gamiz-Gracia L. Journal of Chromatography A. 2013;1315:1‐7
81 - Merdassa Y, Liu J, Megersa N. Talanta. 2013;114:227‐234
82 - Wang X, Mao X, Yan A, Tan T, Yang Y, Wan Y. Food Analytical Methods. 2016;9:3268‐3277
83 - Papadakis E, Kyrgidou A, Vryas Z, Papadopoulou-Mourkidou E. Food Analytical Methods. 2014;7:1271‐1277
84 - Zeng S, Wu H, Li Z, Wang J, Zhang H, Qian M. Journal of Separation Science. 2015;38:121‐127
85 - Kadir HA, Abas F, Zakaria O, Ismail IS, Lajis NH. Analytical Methods. 2015;7:3141‐3147
86 - Lorenzo RA, Racamonde PI, Garcia-Rodriguez D, Carro AM. Analytical and Bioanalytical Chemistry. 2012;404:173‐181
87 - Masia A, Vasquez K, Campo J, Pico Y. Journal of Chromatography A. 2015;1378:19‐31
88 - Van Emon JM, Chuang JC. Analytica Chimica Acta. 2012;745:38‐44
89 - Wang D, Weston DP, Ding Y, Lydy MJ. Archives of Environmental Contamination and Toxicology. 2010;58:255‐267
90 - Zhang Y, Yang J, Shi R, Su Q, Gao Y, Zhu X. Chromatographia. 2011;73:385‐391
91 - Wu G, Bao X, Zhao S, Wu J, Han A, Ye Q. Food Chemistry. 2011;126:646‐654
92 - Homazava N, Aquillon CG, Vermeirssen E, Werner I. International Journal of Environmental Analytical Chemistry. 2014;94:1085‐1099
93 - Kock-Schulmeyer M, Olmos M, de Alda ML, Barcelo D. Journal of Chromatography A. 2012;1305:176‐187
94 - Leyva-Morales JB, Valdez-Torres JB, Bastidas-Bastidas PJ, Betancourt-Lozano M. Journal of Chromatographic Science. 2015;53:1623‐1630
95 - Celeiro M, Llompart M, Lamas JP, Lores M, Garcia-Jares C, Dagnac T. Journal of Chromatography A. 2014;1343:18‐25
96 - Chitescu CL, Oosterink E, de Jong J, Stolker AAM. Talanta. 2012;88:653‐662
97 - Gao X, Pan M, Fang G, Jing W, He S, Wang S. Analytical Methods. 2013;5:6128‐6134
98 - Shi Z, Hu J, Zhang S, Liang Y, Zhang H. Journal of Chromatography A. 2014;1355:219‐227
99 - Wanibrahim W, Nodeh HD, Aboul-Enein HY, Sanagi MM. Critical Reviews in Analytical Chemistry. 2015;45:270‐287
100 - Sun T, Yang J, Li L, Wang X, Li X, Jin Y. Chromatographia. 2016;79:345‐353
101 - Chen F, Song Z, Nie J, Yu G, Li Z, Lee M. RSC Advances. 2016;6:81877‐81885
102 - Zare M, Ramezani Z, Rahbar N. Journal of Chromatography A. 2016;1473:28‐37
103 - Lucci P, Nunez O. Journal of Separation Science. 2014;37:2929‐2939
104 - Liao H, Hsieh C, Chiang S Lin M, Chen P, Wu K. Journal of Chromatography B. 2011;879:1961‐1966
105 - Sancho JV, Pozo OJ, Hernandez F. Rapid Communications in Mass Spectrometry. 2000;14:1485‐1490
106 - Mann O, Pock E, Wruss K, Wruss W, Krska R. International Journal of Environmental Analytical Chemistry. 2016;96:353‐372