Open access peer-reviewed chapter

Bioconvective Linear Stability of Gravitactic Microorganisms

By Ildebrando Pérez-Reyes and Luis Antonio Dávalos-Orozco

Submitted: November 12th 2018Reviewed: December 21st 2018Published: January 28th 2019

DOI: 10.5772/intechopen.83724

Downloaded: 181

Abstract

Interesting results on the linear bioconvective instability of a suspension of gravitactic microorganisms have been calculated. The hydrodynamic stability is characterized by dimensionless parameters such as the bioconvection Rayleigh number R, the gyrotaxis number G, the motility of microorganisms d, and the wavenumber k of the perturbation. Analytical and numerical solutions are calculated. The analytical one is an asymptotic solution for small wavenumbers (and for any motility number) which agrees very well with the numerical solutions. Two numerical methods are used for the sake of comparison. They are a shooting method and a Galerkin method. Marginal curves of R against k for fixed values of d and G are presented along with curves corresponding to the variation of the critical values of Rc and kc. Moreover, those critical values are compared with the experimental data reported in the literature, where the gyrotactic algae Chlamydomonas nivalis is the suspended microorganism. It is shown that the agreement between the present theoretical results and the experiments is very good.

Keywords

  • bioconvection
  • hydrodynamic stability
  • Galerkin method

1. Introduction

Since many years ago, efforts in the experimental and theoretical investigation of the bioconvection phenomenon have been made. These efforts, which lead to the understanding of bioconvective instability, have produced novel and interesting applications. For example, Noever and Matsos [1] proposed a biosensor for monitoring the heavy metal Cadmium based in bioconvective patterns as redundant technique for analysis, a number of researchers [2, 3, 4, 5, 6] have been working on the control of bioconvection by applying electrical fields (as in galvanotaxis) to use it as a live micromechanical system to handle small objects immersing in suspensions, Itoh et al. [7, 8] use some ideas of bioconvection in a study for the motion control of microorganism groups like Euglena gracilis to manipulate objects by using its phototactic orientation (as in phototaxis), and more recently possibly bioconvection seeded the investigation of Kim et al. [9, 10] for using a feedback control strategy to manipulate the motions of Tetrahymena pyriformis as a microbiorobot, among others. Perhaps, further applications on biomimetics [11, 12, 13] at the nano- and microscale could be driven by this contribution.

The term bioconvection was first coined by Platt [14] as the spontaneous pattern formation in suspensions of swimming microorganisms. This phenomenon has some similarity with Rayleigh-Benard convection but originates solely from diffusion and the swimming of the organisms. Reviews about this topic have been published by Pedley and Kessler [15] and Hill and Pedley [16]. Ideas and theories on cellular motility can be found in the book of Murase [17], and the effect of gravity on the behavior of microorganisms is widely explained in the book of Hader et al. [18]. In 1975, Childress et al. [19] presented a model for bioconvection of purely gravitactic microorganisms and their results of a linear theoretical study, and later Harashima et al. [20] studied the nonlinear equations of this model. According to the model of Childress et al. [19], the critical wavenumber at the onset of the instability is always zero. In ordinary particles and colloidal suspensions, the internal degrees of freedom like the internal rotation or spin are important under some geometrical and physical conditions [21, 22]. The case of a suspension of microorganisms is not an exception. For this case, Pedley et al. [23] proposed a gyrotactic model for a suspension of infinite depth. Their model includes the displacement of the gravity from the geometric center in the organisms along their axis of symmetry. Hill et al. [24] performed an analysis of the linear instability of a suspension of gyrotactic microorganisms of finite depth using the model of Pedley et al. [23]. Hill et al. [24] found finite wavenumbers at the onset of the instability, but agreement with the experiment was not good. Later, Pedley and Kessler [25] reported a model for suspensions of gyrotactic microorganisms where account was taken of randomness in the swimming direction of the cells. In a study of the linear instability of the system based on the model of Pedley and Kessler [25], Bees and Hill [26] found disagreement between their theoretical results and the experimental data reported by Bees and Hill [27]. Several experimental investigations of bioconvection have been reported by Loeffer and Mefferd [28] and Fornshell [29], by Kessler [30] and Bees and Hill [27] who take into account the gyrotaxis, by Dombrowski et al. [31] and Tuval et al. [32] who take into account the oxitaxis, and more recently by Akiyama et al. [33] who observed a pattern alteration response characterized by a rapid decrease in the bioconvective patterns. Pattern formation has been observed in cultures of different microorganisms such as Chlamydomonas nivalis, Chlamydomonas reinhardtii, Euglena gracilis, Bacillus subtilis, Paramecium tetraurelia, and Tetrahymena pyriformis.

More recently, investigations have been reported for a semi-dilute suspension of swimming microorganisms where cell–cell interactions are considered [34, 35, 36, 37, 38]. On the other hand, Kitsunezaki et al. [39] investigated the effect of oxygen and depth on bioconvective patterns in suspensions with high concentrations of Paramecium tetraurelia. Bioconvection is also studied from other points of view in gravitational biology. Interesting results are also available in Refs. [40, 41, 42] about the pattern formation in suspensions of Tetrahymena and Chlamydomonas subject to different gravity conditions. Further results are due to Sawai et al. [43] who investigate the proliferation of Paramecium under simulated microgravity, to Mogami et al. [44] who report an investigation of the formed patterns by Tetrahymena and Chlamydomonas as well as a physiological comparison, to Takeda et al. [45] who give an explanation of the gravitactic behavior of single cells of Paramecium in terms of the swimming velocity and swimming direction, to Mogami et al. [46] who present theory and experiments of two mechanisms of gravitactic behavior for microorganisms, and to Itoh et al. [47] who investigate the modification of bioconvective patterns under strong gravitational fields.

This chapter presents interesting results about the bioconvective linear stability of a suspension of swimming microorganisms. Use is made of the equations presented by Ghorai and Hill [48, 49] some years ago. In their approach, Ghorai and Hill [48, 49] used a different dimensionalization scale for the concentration microorganisms which gives distinct meaning to the basic state for the concentration of microorganisms and a bioconvective Rayleigh number defined in terms of the mean cell concentration. To the authors best knowledge, those equations along with the change in the basic state and Rayleigh number definitions have not been used to determine the linear bioconvective instability in an infinite horizontal fluid layer and to compare the results with experiment. These results were obtained by means of both numerical and analytical techniques. The critical values of the Rayleigh number Rcand the wavenumber kc, for fixed values of the gyrotaxis number G and the motility of microorganisms d, that characterize the hydrodynamic stability of the system are compared with the experimental data presented in Table I of Bees and Hill [27] and Table II of Bees and Hill [26] where the gyrotactic biflagellate alga Chlamydomonas nivalis is used as suspended microorganism. Below, it is shown for the first time that the numerical results have a very good agreement with the experimental data.

The chapter is organized as follows. The governing equations and boundary conditions [48, 49] as well as the basic state can be found in Section 2. Nondimensionalization and linearization of the system of equations is outlined in Section 3. In Section 4, use is made of an asymptotic expansion [50, 51, 52, 53] method and a Galerkin method [54] to find limiting cases and predict critical values of R and k for the instability. The numerical calculations done by means of the shooting method along with the graphics corresponding to the marginal curves are given in Section 5. In Section 6, the experimental data [27] are compared with the numerical results. A discussion is given in the final section.

2. Equations of motion

We consider an infinite horizontal layer of a suspension of gyrotactic microorganisms. The fluid layer is bounded at z=H,0. The fluid where the cellular microorganisms swim is water with density ρ. Each cell has a volume υand density ρ+Δρ, where Δρρ. The suspension is considered dilute and incompressible. Density fluctuations in the suspension are small enough such that the Boussinesq approximation is valid and the corresponding governing equations are

ρDuDt=pnυgΔρk+μ2uE1
nt=JE2
u=0E3

where tis the time, uis the suspension velocity, pis the pressure, gk is the acceleration due to gravity, k is the vertical unit vector, μis the viscosity, nis the concentration of microorganisms, and Jis the flux density of organisms through the fluid defined as

J=nu+nVcpDcnE4

where Vcis the cell swimming speed, pis a unit vector representing the average orientation of cells, and Dcis a scalar microorganism mass diffusion coefficient independent of the other parameters of the problem. Use is made of Cartesian coordinates with the z-axis in the vertical direction. The walls at z=H,0are considered to be rigid. As pointed out by Hill et al. [24] although the top boundary is open to the air, algal cells tend to collect at the surface forming what appears to be a packed layer, and it is unlikely that the boundary is ever fully stress-free. Then the boundary conditions are

u=0atz=H,0E5
Jk=0atz=H,0E6

In the basic state, the fluid velocity is zero and n=n0zand p0=k. Thus for n0zfrom Eq. (2) with the boundary conditions (6), we have

n0z=n¯VcHexpVcz/DcDc1expVcH/DcE7

where n¯represents the average concentration of organisms. Eq. (7) is the same basic state as presented by Ghorai and Hill [48, 49] whose linear stability will be investigated. It differs from that of Childress et al. [19] and Hill et al. [24] by the coefficient

VcHDc1expVcH/Dc

3. Linear stability

We make the governing Eqs. (13) nondimensional by scaling all lengths with H, the time with H2/Dc, the fluid velocity with Dc/H, the pressure with νDcρ/H2, and the cell concentration with n¯HVc/Dc. Now the dimensionless variables are expressed without star. The boundaries are located at z=1,0and the basic state is

u0=0,p0=k,n0z=edz1ed

where the nondimensional quantity d=VcH/Dcis the ratio of swimming speed of microorganisms and their representative mass diffusion velocity. Here, d is called the motility of the microorganisms. In order to investigate the linear stability of the system, small perturbations have to be considered. They are

u=u0+δu1,p=p0+δp1,p=p0+δp1,n=n0+δn1

where δ1. The components of u1 are u1v1w1. In this way, the nondimensional governing Eqs. (13) are linearized to order Oδ. Then, we have the following linear equations

Sc1u1t=pRn1k+2u1E8
n1t=w1dn0dzdn1zdn0p1+2n1E9
u1=0E10

where

Sc=νDc,R=n¯υgΔρH3dDcνρ

are the Schmidt and bioconvection Rayleigh numbers, respectively. Pedley and Kessler [55] give a definition of the vector p1for swimming microorganisms with spheroidal shape. They determine p1in terms of u1that in nondimensional form is

p1=G1+α0u1z1α0w1E11
G=BDcH2E12

where the subscript denotes the horizontal component, α0is the cell eccentricity, and Gis the nondimensional form of the gyrotactic orientation parameter B. Finally after substitution of p1and n0, the governing equations become

Sc1ut=pRnk+2uE13
nt=dedz1edw+G1+α02wz2+1α02w])dnz+2nE14
u=0E15

with boundary conditions

u=0atz=1,0andnzdn=0atz=1,0E16

where the superscripts have been deleted. Notice that the adimensionalization of the equations is different from that of Hill et al. [24]. Here, an application of a more general asymptotic analysis for any magnitude of d is used. An analytic Galerkin method and a shooting numerical method for the solution of the proper value problem allowed us to have an interesting perspective of the stability of the present problem under research. The results are used here to compare with the experimental data of the flagellated alga Chlamydomonas nivalis.

By elimination of the pressure from Eqs. (1315), it is possible to obtain a coupled system of two equations, for w and n, to describe the instability of the system. The perturbations of the variables will be analyzed in terms of normal modes of the form

w=Wzexpkxx+kyyi+σt,n=Φzexpkxx+kyyi+σt

where k=kx2+ky2is the wavenumber of the disturbance and σis the growth rate. The wavenumber is scaled as k=kHcorresponding to a nondimensional wavelength λ=2π/k. Thus, the governing equations become

Rk2Φ=σSc+k2D2k2D2WE17
σ+dD+k2D2Φ=dedz1ed1+G1+α0D21α0k2WE18

subject to the boundary conditions

W=DW=DΦdΦ=0atz=1,0E19

where D=d/dz. The variables of the above problem can be changed in order to simplify the analysis. The change of dependent variable is

Φ=Fzedz

Then, Eqs. (17) and (18) and the boundary conditions Eq. (19) become

Rk2Fedz=σSc+k2D2k2D2WE20
σdD+k2D2F=d1ed1+G1+α0D21α0k2WE21

subject to the new boundary conditions

W=DW=DF=0atz=1,0E22

In this form, the equations are very similar to those of the well-known problem of thermal convection in an infinite horizontal fluid layer between nonconducting boundaries [50, 51, 52, 53, 56]. The familiar fixed heat flux boundary condition is the main characteristic of those thermal convection problems and is analogous to that presented in Eq. (22). The equations derived by Childress et al. [19] can also be analyzed from the present view point of this change of variable. In the theory of thermal convection as in that of Childress et al. [19], a zero critical wavenumber is found as a result of the fixed flux boundary condition. In more recent models, which include the effects of gyrotaxis, the similarity with the thermal convection problem is not valid unless G=0.

4. Asymptotic analysis

In this section, the eigenvalue problem stated in the system of Eqs. (1315) with boundary condition Eq. (16) is investigated by means of two analytic methods. The magnitude of the marginal value of R is a function of all the other parameters. The way in which the solution of the stability problem is to be carried out is as follows. For a given value of d and G, we must determine the lowest value for R with respect to the wavenumber k. The values obtained are the critical Rayleigh numbers Rc at which instability will first occur.

4.1 Asymptotic analysis

We conducted a general asymptotic analysis in comparison with those used before [19, 24, 26] which included the restrictions of the limits d1for shallow layers and d1for deep layers along with different restrictions for G. In a similar way, as in other problems of convection, we follow the steps of Chapman and Proctor [51], Dávalos-Orozco [52], and Dávalos-Orozco and Manero [53]. Under the above conditions, the analysis is very complex, the reason why use has been made of the Maple algebra package. Thus, we look for a solution to Eqs. (20) and (21) using the following expansions:

W=W0+εW1+,E23
Φ=Φ0+εΦ1+,E24
R=R0+εR1+,E25
σ=εσ0+ε2σ1+E26

where ε1. We also consider no restrictions for dand Gand rescale the wavenumber as k=ε1/2k˜. Thus, after substitution of expansions Eqs. (2326) and the mentioned scalings in Eqs. (20) and (21) with boundary condition Eq. (22), we obtain the following systems of equations at different orders.

At order O1

D4W0+k˜2R0edzF0=0,E27
D2+dF0=0E28

subject to

W0=DW0=DF0=0atz=1,0.E29

At order Oε

D4W12k˜2+σ0ScD2W0+k˜2edzR0F1+R1F0=0,E30
d1edG1+α0D21W0σ0+k˜2F0+D2+dF1=0E31

subject to

W1=DW1=DF1=0atz=1,0.E32

At order Oε2

D4W22k˜2+σ0ScD2W1+k˜4+σ0k˜2Scσ1ScD2W0+k˜2edzR0F2+R1F1+R2F0=0,E33
d1edG1+α0D21W1G1+α0k˜2W0σ0+k˜2F1σ1F0+D2+dF2=0E34

subject to

W2=DW2=DF2=0atz=1,0.E35

The systems of equations at order Oεand higher are inhomogeneous and must satisfy their corresponding solvability conditions allowing to compute the Rayleigh number R as an eigenvalue in terms of the other parameters of the problem. Solvability conditions are found as usual [57]: each inhomogeneous system is multiplied by the solution to the adjoint of the homogeneous system and integrated over the range of the independent variable. The resulting integral must vanish.

Thus, the solvability conditions at Oεand Oε2are, respectively,

0=d1ed10edzG1+α0D21W0dzσ0+k˜210edzF0dzE36
0=d1ed10edzG1+α0D21W1G1+α0k˜2W0dzσ0+k˜210edzF1dzσ110edzF0dzE37

The solutions of the system of equations at leading order are

F0=1,W0=f1zdR0k˜2E38

where the function f1zdcan be obtained from the authors upon request. For convenience, the solution F0has been normalized to 1. The next step is to evaluate the solvability condition Eq. (36) at Oεand obtain an expression for σ0

σ0=k˜21d2G1+α0A0R01E39

The constant A0is large and can be obtained from the authors upon request. At order Oεsimilar steps as those for solving the system of equations at O1are followed to find F1and W1. Then, algebraically F1is

F1=G1+α0f2zd+f3zdR0k˜2E40

After substitution of F0, W0, F1, and σ0into Eq. (30), the velocity W1can be calculated subject to its corresponding boundary condition Eq. (32). Because of the term edzappearing in the system of equations at O1, the expression of W1is very large and complicated and will not be given here. The evaluation of the solvability condition at order Oε2given in Eq. (37) yields

σ1=1d2G1+α0k˜2A0R1G21+α02A1+G1+α0A2+A3R02+G1α0A4R0+R01d2G1+α0G1+α0A5+A6R0+A7+1d2G1+α0A8R0+A7/2/Sc}k˜4E41

The growth rate can now be obtained by substitution of σ0and σ1into the expansion for σgiven in Eq. (26). However σis omitted to save space but can be obtained from the authors upon request. Finally, use is made of the expansion Eq. (25) for R.

Now, the transition from stability to instability via a stationary state is investigated by setting σ=0. Thus, the corresponding value of Rfor the marginal state is

R=11d2G1+α0A0+k21d2G1+α0A0G1α01d2G1+α0+G1+α0A5+A61d2G1+α0A02+G21+α02A1+G1+α0A2+A31d2G1+α02A02+A7A0E42

where some simplifications have been made with the use of R0obtained from Eq. (39). The functions f2zdand f3zdand the coefficients A1to A8appearing in the above expressions are functions of dand can be obtained from the authors upon request. The result for R0is

R0=11d2G1+α0A0E43

From the expression for the Rayleigh number given in Eq. (42), it is possible to calculate the limit for d1. In this case, we consider dand kto be of the same order in such a way that kd=k/dand kdO1. Then, under these assumptions, the approximation of RkdGα0is

R=7201+d217420+G1+α0+kd21746227G52α0+Od3E44

Here we point out that in the present chapter, our definition of the Rayleigh number differs from that defined by Hill et al. [24]. If our approximation given in Eq. (44) is multiplied by d1ed=1+d/2+d2/12d4/720, the same approximation by Hill et al. [24] is obtained. Moreover, if G=0this becomes that given by Childress et al. [19]. In the more general expression of Rfor a small wavenumber approximation, Eq. (42) has a special characteristic due to its dependence on the square of the wavenumber k. The first coefficient at zeroth order in kcorresponds to R0, and that at the second order in kis R1. Even though in the experiments on bioconvection [27], only finite critical wavenumbers kc>0have found these coefficients are very useful. For example, it can be shown from R0that if A0>0and

1d2G1+α0>0E45

then R0<0. This corresponds to a stable stratification, which is not the case here. The second coefficient R1in Eq. (42) is a very important result, because it provides information about the shape of the marginal curve with respect the critical wavenumber. That information can be obtained by making zero the coefficient R1and calculating the following critical value of the gyrotaxis number Gc

0=G21+α02A1d2A5d2A021α01+α0+G1+α0[A2+A5d2A6+A7A0+A021α01+α0+A3+A6+A7A0E46

From this equation, two admissible cases are possible when Eq. (45) is satisfied. First, for fixed values of d, α0, and G>Gc, the marginal curve starts at k=0and then decreases monotonically. However, according to the numerical analysis presented below, the marginal curves in fact first decrease and then start to grow monotonically after a minimum is attained, at the critical wavenumber. In the second case, for fixed values of d, α0, and G<Gc, the marginal curves start at k=0and then grow monotonically. Here, the critical wavenumber is always zero. The importance of these results is that the magnitude of Gcagrees very well with the results of the marginal curves found in the numerical analysis given below. This critical value determines the magnitude for which the curves have a finite critical wave number (G>Gc) or a zero critical wavenumber (G<Gc). It is of interest to note that some of the magnitudes of the gyrotaxis parameter Gcalculated from the data in the literature are very near but above of Gc. This is the reason why some of the curves found from numerical analysis are almost flat in a range of wavenumbers near to zero. Because the experimental critical wavenumbers found for gyrotactic bioconvection are always finite, we conclude that Gcis important to point out where to find the theoretical limitations of the model.

4.2 Analytic Galerkin method

Here use is made of the analytical Galerkin method to study the eigenvalue problem of Eqs. (17)(18) with the boundary condition Eq. (19). This method has been used before by Pellew and Soutwell [58], Chandrasekhar [54], and Gershuni and Zhukovitskii [59]. Even though this is an approximate method, it has a very high precision. The advantage of the method is that it is possible to obtain an explicit expression of the Rayleigh number R. Here, it is supposed that σ=0.

Briefly, the method consists in assuming a trial function which satisfies the boundary conditions for each of the dependent variables. Let that variable be Φwhich after substitution in one of the equations of the problem allows for the exact solution of the other variables, let us say W. Both trial functions are now substituted into the other coupled equation. Then, use is made of the orthogonality properties of the solutions in this equation to obtain the proper value of the Rayleigh number as a function of the other parameters [60].

In this way, the proposed expansions of Φand Ware

Φ=n=0BnexpdzcosπnzandW=n=0BnWnE47

then, after substitution of Φof Eq. (47) into Eq. (20), Wnis the solution of the following differential equation:

D2k22Wn=Rk2expdzcosπnzE48

which is subjected to the conditions

Wn=DWn=0atz=1.0.E49

The solution is

Wn=a1cosπnz+a2sinπnzedzk2R+c1ekz+c2ekz+c3zekz+c4zekzE50

where c1to c4are constants of integration which can be found by evaluating in the boundary condition Eq. (49).

Next, Eq. (18) is multiplied by Φand is integrated in the range z=1to z=0, to get

d1ed10Φ1+G1+α0D21α0k2Wdz10ΦDedzDk2edzΦdz=0E51

After substitution of Φand W, given in Eqs. (47) and (50) and some simplifications, we obtain

d1ed10Φm1+G1+α0D21α0k2Wndz10ΦmDedzDk2edzΦndz=0E52

This determinant, calculated with the help of the software Maple, is the solvability condition from which the eigenvalue Ris calculated. The resulting algebraic expression of the integrals in this equation is very complex and will not be presented here. However, the first approximation of R, corresponding to the element (0,0) of the matrix in the determinant Eq. (52), is

R=1edA10ded1+G1α0k2d2G1+α0A9E53

where A9and A10are functions of the wavenumber k and d and can be obtained from authors upon request. This result is new because it includes, for the first time, all the parameters of the problem without any approximation. In the limit of d,k,G0, R reduces to the well-known value of 720. Higher-order estimates of R can be obtained from Eq. (52), which provides a useful check on numerical calculations. The comparison of Rgiven in Eq. (42), obtained from the asymptotic analysis, and that of Eq. (52) shows that in the limit k0, the agreement was very good.

5. Numerical computations by a shooting method

Here, the shooting method [61] is used to solve the eigenvalue problem posed by the system of Eqs. (20) and (21) subjected to the boundary condition Eq. (22). Curves of marginal stability in the plane kRwere calculated for fixed values of the parameters d, G, and α0. Notice that very good agreement was always found among the values of the Rof the asymptotic analysis (in the limit k0), of the Galerkin method, and of the numerical computations. Calculations were made in two ways. First, the parameters d, G, and α0were varied in order to obtain a representative set of marginal curves for the problem of bioconvection. Second, experimental data were also used to fix the values of d, G, and α0and used to find theoretical values of kcand Rcthat could be compared with their corresponding experimental values. Here, in particular, a selection is made of α0=0.4, which corresponds to the flagellated alga Chlamydomonas nivalis. Figures 13 show marginal curves for different values of the gyrotaxis parameter G, while dremains fixed with magnitudes 0.1, 1, and 5, respectively. These figures clearly show the effect the gyrotaxis parameter Ghas on the critical wavenumber. When the magnitude of Gis large enough, the critical wavenumber changes from zero to a finite value which increases with G, as shown by the squares located at the minimum value of R. Notice that it is found that the critical value Gc, which represents the magnitude at which the properties of the marginal curves change, from having kc=0to kc>0, is very well approximated by Eq. (46). This critical value is important because it represents the magnitude of Gbelow which the present theory ceases to predict the experimental results which always show critical wavenumbers kc>0.

Figure 1.

(a) Graphs of log R vs. k for fixed d = 0.1 . (b) Graphs of log R vs. k for fixed d = 1 . The black square markers indicate the position of the critical wavenumber and Rayleigh number.

Figure 2.

(a) Graphs of log R vs. k for fixed d = 5 . (b) Graphs of log R vs. k for experiments 35, 2, 4, and 9 with d increasing from the curve below to above. The black square markers indicate the position of the critical wavenumber and Rayleigh number.

Figure 3.

(a) Graphs of log R vs. k for experiments 26–29, 24, 31, and 16 with d increasing from the curve below to above. (b) Graphs of log R vs. k for experiments 13, 10, and 20 with d increasing from the curve below to above. The black square markers indicate the position of the critical wavenumber and Rayleigh number.

In the curves shown in Figure 1a–b, the critical values of the gyrotaxis parameter are Gc=0.0306,0.0266,0.0060, respectively. As mentioned above for G>Gc, the critical wavenumber is finite, and for G<Gcthe critical wavenumber is always zero. The combined effects of the velocity of the swimming of microorganisms, d, and that of gyrotaxis, G, change the location of the critical wavenumber. Note also that for fixed d, when Gincreases, the system becomes more unstable. From the Figure 1a and b, it can be seen that the most unstable case corresponds to that for d=0.1and G=10where kc=4.45and Rc=9.0618. This may be understood by the fact that the accumulation of microorganisms near to the top of a shallow layer is faster than in a deeper one. This is due to the important role that the mass diffusion of microorganism Dcand the depth of suspension Hplay on the instability of the system. The value of Gcin the limit of d,k0can also be calculated from Eq. (46) by means of an asymptotic analysis. That is,

Gc=1713252α0+Od2E54

Here, some theoretical curves are presented of which some have a very good agreement and others a reasonable agreement with the experiments 2, 4, 9, 10, 13, 16, 20, 24, 26, 27, 28, 29, 31, and 35, performed by Bees and Hill [27].

The values for the motility dand the gyrotaxis parameter Gused in Figures 2 and 3 were calculated based on experimental data by Bees and Hill [26, 27], which in here are presented in Tables 2 and 3 of the following section. In order to observe in detail the position of the critical point kcRcin Figures 2 and 3, a local magnification is included.

Here, a comparison is done of our theoretical results of kcRcwith the theoretical ones presented by Bees and Hill [26] in their Table VI. According to Bees and Hill [26], experiments 2 and 23 in their Table V have kcRcof comparable order with those in their Table VI. In our Table 1, we reproduce the comparison made by Bees and Hill [26] of their own theoretical and experimental results of their Table V, and we added the corresponding error in percent of the wavenumbers and Rayleigh numbers, respectively. Note that the value α0=0.4corresponds to flagellated microorganisms such as Chlamydomonas nivalis, while α0=0.2corresponds to nonflagellated. Notice that their experimental and theoretical values of dare not exactly the same.

Experimental resultsTheoretical predictionsError (%)
ENdBHkcRBHc×106dBHdBH2ηα0kcRBHc×106kRBH
244.75.113.2540160.2515.089853
40160.4519.0898176
232047.84863200320.22701700334396

Table 1.

Experimental measurements of Bees and Hill [27] and their theoretical prediction [26].

EN represents the experiment name. Subscript BH indicates that the definition of Bees and Hill, [26] for the parameters dand Ris used. ηis the gyrotactic parameter [26].

For the sake of comparison of our theoretical results with those of the experiments, Table 1 shows the percent of error calculated by taking the difference of the experimental and theoretical values and then dividing by the smallest one. In Table 1, the more realistic value α0=0.4for Chlamydomonas nivalis is included, which corresponds to the second line of experiment 2 of Bees and Hill [26] predictions. It is clear from Table 3 that our theoretical results show a very important improvement in the reduction of the percent error with respect to experiment 2.

6. Comparison with experiments

In this section a comparison is done of our theoretical results of Rcand kcwith the corresponding experimental values obtained by Bees and Hill [27]. Here use is made of the results of the 39 experiments shown in Table I of Bees and Hill [27]. Besides, the more realistic value of the parameter α0=0.4, corresponding to the flagellated algae Chlamydomonas nivalis, is also used to calculate d, G, and R.

In Table 3, the values of d, G, and Rresulting from the experimental data are presented. Note in Table 2 that the experimental results of the cell swimming speed Vsand of the cell diffusivity Dcare given inside a range of values. In this case, a particular value inside the range has to be selected. The swimming speed used here is 63×104cm/s. The decision is based on the results obtained by Hill and Hader [62], Pedley and Kessler [25], and Bees and Hill [26]. The value of the cell diffusivity was decided to be that corresponding to an average over the range given in Table 2, that is, Dc=27.5×105cm2/s.

NameDescriptionValue
ϑCell volume5 × 10−10 cm3
gAcceleration due to gravity103 cms−2
DcCell diffusivity5 × 10−5–5 × 10−4 cm2s−1
ρFluid density1 gcm−3
ρ+ΔρCell density1.05 gcm−3
νKinematic viscosity10−2 cms−2
VcCell swimming speed0–2 × 10−2 cms−1
BDimensional gyrotaxis parameter3.4 s
BIncluding flagella6.3 s
α0Cell eccentricity0.20–0.31
α0Including flagella0.4

Table 2.

Estimates and measurements of typical parameters for a suspension of the alga Chlamydomonas nivalis [24, 25, 64, 65].

Very recent experimental measurements on the diffusivity for different microorganisms like the biflagellated alga Chlamydomonas reinhardtii have been reported by Polin et al. [63]. Bees and Hill [27] state that there is some evidence to suggest that cells of Chlamydomonas nivalis are not gyrotactic during the first week of subculturing; then if it is not the case for the cells of Chlamydomonas reinhardtii, more measurements for the parameters α0, B, Vs, H, n¯, and kcwould be needed in order to perform comparison between theoretical and experimental results. The definitions of d, G, and Rare related with those of Bees and Hill [26] dBH, η, and RBH, respectively, as follows:

d=Vs2τK2DcK1dBH,G=DcVs2τη,R=K22τ3Vs5Dc2HK121expK1HK2VsτRBHE55

where the constants K2=0.15and K1=0.57(see [26] for more details). τis a direction correlation time which equals 1.3sin the nonflagellated case and 5sin the flagellated case. The data corresponding to the suspension depth Hand the average cell concentration of microorganisms n¯of each experiment (see [27] for more details) have not been reported in Table 3. Only the parameters d, G, k, and Rare presented in that table. It is also found that the value of the Gof each experiment is greater (but sometimes near) than their corresponding critical value Gcof Eq. (46). Under these conditions, all the critical wavenumbers have to be kc>0.

NEdG×102RERTkEkTError k (%)Error R (%)
17.631.567043.6410384.235.675.650.35347.6
29.071.1010599.1417075.285.126.2221.561.1
38.361.3023319.6213531.068.595.9544.472.3
410.20.87924835.7823783.255.966.7112.64.42
511.90.63612433.0337984.116.817.6912.9205
616.70.32659993.82105787.086.6110.964.976.3
79.141.094676.3417544.336.016.314.99275
88.731.197198.4715309.027.256.0919.0112
910.40.83320618.7825437.608.336.8421.823.4
1015.80.36488709.3789546.438.3410.323.50.943
116.462.183700.786613.535.245.311.3378.7
1212.10.62139993.6840261.725.667.9440.30.670
1314.80.41677622.3273779.007.859.7123.75.20
148.801.178561.4115655.836.936.1113.482.9
157.281.714022.379146.745.465.541.46127
167.101.806960.808540.986.435.4917.122.7
1710.70.78818965.8127708.924.167.0268.746.1
1810.70.78818965.8127708.928.327.0218.146.1
1910.70.78818965.8127708.924.897.0243.546.1
2016.60.331107793.38104192.948.710.925.33.45
218.801.178561.4115655.837.016.1114.782.9
228.131.376910.8112466.946.115.844.6280.4
2310.70.79141777.5227799.287.847.0611.050.3
246.672.046260.757202.626.075.3613.215.0
254.265.011044.172301.466.224.8229.0120
266.462.185663.126613.536.715.3126.416.8
276.462.185663.126613.536.055.3113.916.8
286.462.185663.126613.535.375.311.1316.8
296.462.185663.126613.535.945.3111.916.8
307.831.4833599.2411220.817.465.7430.0199
316.801.966478.077585.386.005.3911.317.0
324.474.564519.702583.996.664.8637.074.9
332.7012.4475.882180.734.976.5231.2358
343.856.141958.294879.036.216.464.02149
357.421.658259.409655.916.155.609.8216.9
367.831.4833599.2411220.816.495.7413.1199
375.223.332418.533794.616.374.9927.656.9
386.871.9220572.5322011.5110.47.1346.06.99
396.871.9220572.5322011.5110.87.1351.96.99

Table 3.

Experimental measurements of wavenumbers [27] and present theoretical predictions.

EN means experiment name, and subscripts E and T indicate experimental and theoretical data. Cell eccentricity α0=0.4is used.

By using the data of our Table 2 and Table I of Bees and Hill [27], the experimental values for d, G, and REwere calculated and listed in Table 3. The experimental value of the wavenumber kEwas also obtained from Table I of Bees and Hill [27] and was calculated as follows: the wavelength λ0cmis nondimensionalized with the corresponding suspension depth Hcmto get λE, and then the critical wavenumbers were calculated from kE=2π/λE. RTand kTare our theoretical wavenumber and Rayleigh number obtained by the shooting method. The curves of marginal stability corresponding to experimental results with good and very good agreement with theory are shown in Figures 2 and 3. As explained above, we have a substantial improvement in the agreement of the critical wavenumbers and Rayleigh numbers with respect to the experimental results (see Table 3). A great number of experimental data have been compared with the present theory in Table 3.

Some numerical results agree very well with experiments, as can be seen in the experiments 4, 10, 12, 13, 20, and 35 of Table 3. Others are good, such as the results of experiments 9, 16, 24, 26, 27, 28, 29, and 31. With respect to the other data in Table 3, it might be possible that if the experimental measurements are improved, the agreement with theory will be better. The results given here show that the approximate and numerical solutions of the system of governing equations presented in this paper may bring a light to the solution of many other problems of bioconvection.

7. Conclusions

The governing equations of bioconvection were used to investigate the problem of an infinite horizontal microorganism suspension fluid layer. The theoretical predictions of the critical wavenumber kcand Rayleigh number Rcwere compared with their experimental counterparts [27]. Very good, good, and fair agreements were found. But in general, we may say that our numerical results improve by far those obtained by Bees and Hill [26].

With the asymptotic analysis for k<<1, it was possible to calculate a Rayleigh number not reported before without any restrictions on the magnitudes of d and G. This result is important because it was also possible to calculate a critical value of the gyrotaxis parameter Gcwhich indicates the boundary between the possibility of a marginal curve with kc=0(G<Gc) and another one with kc>0(G>Gc).

However, it is clear from the experimental results that the critical wavenumbers are finite and large and that the former case is not physical. Therefore, this Gcalso defines the limit of validity of the theory. Note that it agrees very well with numerical analysis.

An analytic Galerkin method was also used to obtain a general expression of R without any restriction on the magnitudes of d, G, and kO1. This gave us an explicit expression of R not reported before which proved to be very useful when checking with the numerical computations.

Numerical results have shown that the system becomes more unstable when the layers are shallow. The physical interpretation of such situation is that the accumulation of microorganisms near the top of the layer in the shallow case is faster than in the deeper case, due to the smaller depth of suspension H. A consequence of this is that the critical wavenumber is smaller for shallower layers. This can be explained by means of the boundary conditions of the microorganism concentration. If the parameter d tends to zero, the boundary conditions tend to those similar to the “fixed heat flux” boundary conditions of the problem of natural convection heated from below [50, 51, 52, 53, 56]. Moreover, it has been shown above that by a change of variable, it is possible to transform the boundary conditions of the concentration into those similar to the “fixed heat flux” boundary conditions. In that problem it has been shown that the critical wavenumber tends to zero. However, due to the gyrotaxis, the critical wavenumber is not zero in the present problem if G > Gc, which, from the experimental results, is the case here. But notice in Figures 13 that in fact, also in this case, the critical wavenumber decreases with a decrease of d. The change of the critical wavenumber with respect to G is also clear in the figures. The critical wavenumber decreases with a decrease of G.

Finally, we would like to point out that it is our hope that the results presented in this chapter may stimulate researchers to make more new and precise experiments on bioconvection in the near future.

Nomenclature

B

dimensional gyrotactic parameter, s

k

wavenumber

Dc

cell diffusivity, cm2s−1

d

motility of microorganisms

G

dimensionless gyrotactic parameter

g

acceleration due to gravity, cms−2

H

layer depth, cm

J

flux density of organisms

n¯

average cell concentration

n

concentration of microorganisms

p

pressure

R

Rayleigh number

Sc

Schmidt number

t

time

Vc

cell swimming speed, cms−1

u

fluid velocity

x

Cartesian coordinates

Greek symbolsα0

cell eccentricity

μ

viscosity, gcm−1s−1

ν

kinematic viscosity, cm2s−1

ρ

water density, gcm−3

ϑ

cell volume, cm−3

SubscriptsBH

result obtained by Bees and Hill [26]

c

critical value

E

experimental result

T

theoretical result

Acknowledgments

The authors would like to thank Alberto López, Alejandro Pompa, Cain González, Raúl Reyes, Ma. Teresa Vázquez, and Oralia Jiménez for technical support. I. Pérez Reyes would like to thank the Programa de Mejoramiento del Profesorado (PROMEP).

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Ildebrando Pérez-Reyes and Luis Antonio Dávalos-Orozco (January 28th 2019). Bioconvective Linear Stability of Gravitactic Microorganisms, Heat and Mass Transfer - Advances in Science and Technology Applications, Alfredo Iranzo, IntechOpen, DOI: 10.5772/intechopen.83724. Available from:

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