Experimental parameters of the analyzed regular waves.
\\n\\n
More than half of the publishers listed alongside IntechOpen (18 out of 30) are Social Science and Humanities publishers. IntechOpen is an exception to this as a leader in not only Open Access content but Open Access content across all scientific disciplines, including Physical Sciences, Engineering and Technology, Health Sciences, Life Science, and Social Sciences and Humanities.
\\n\\nOur breakdown of titles published demonstrates this with 47% PET, 31% HS, 18% LS, and 4% SSH books published.
\\n\\n“Even though ItechOpen has shown the potential of sci-tech books using an OA approach,” other publishers “have shown little interest in OA books.”
\\n\\nAdditionally, each book published by IntechOpen contains original content and research findings.
\\n\\nWe are honored to be among such prestigious publishers and we hope to continue to spearhead that growth in our quest to promote Open Access as a true pioneer in OA book publishing.
\\n\\n\\n\\n
\\n"}]',published:!0,mainMedia:null},components:[{type:"htmlEditorComponent",content:'
Simba Information has released its Open Access Book Publishing 2020 - 2024 report and has again identified IntechOpen as the world’s largest Open Access book publisher by title count.
\n\nSimba Information is a leading provider for market intelligence and forecasts in the media and publishing industry. The report, published every year, provides an overview and financial outlook for the global professional e-book publishing market.
\n\nIntechOpen, De Gruyter, and Frontiers are the largest OA book publishers by title count, with IntechOpen coming in at first place with 5,101 OA books published, a good 1,782 titles ahead of the nearest competitor.
\n\nSince the first Open Access Book Publishing report published in 2016, IntechOpen has held the top stop each year.
\n\n\n\nMore than half of the publishers listed alongside IntechOpen (18 out of 30) are Social Science and Humanities publishers. IntechOpen is an exception to this as a leader in not only Open Access content but Open Access content across all scientific disciplines, including Physical Sciences, Engineering and Technology, Health Sciences, Life Science, and Social Sciences and Humanities.
\n\nOur breakdown of titles published demonstrates this with 47% PET, 31% HS, 18% LS, and 4% SSH books published.
\n\n“Even though ItechOpen has shown the potential of sci-tech books using an OA approach,” other publishers “have shown little interest in OA books.”
\n\nAdditionally, each book published by IntechOpen contains original content and research findings.
\n\nWe are honored to be among such prestigious publishers and we hope to continue to spearhead that growth in our quest to promote Open Access as a true pioneer in OA book publishing.
\n\n\n\n
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Wave breaking is one of the most important process for coastal engineers since it greatly influences both the transport processes and the magnitudes of the forces on coastal structures [1, 2]. Wave breaking in the surf zone drives complicated turbulent structures and for this reason breaking is possibly the most difficult wave phenomenon to describe mathematically [3, 4, 5].
Pioneering experimental studies were carried out by [6, 7, 8, 9, 10, 11], who described the velocity field under plunging breakers in the outer region of the surf zone; more recently by [12, 13, 14, 15, 16, 17]. As observed experimentally by [18], during the pre-breaking stages, the maximum turbulence intensity appears at the core of the main vortex and decreases as the vortex moves downstream. Additional turbulence is then generated near the free surface during the breaking process.
Moreover, the difficulties of measuring velocity due to the existence of air bubbles entrained by the plunging jet have hindered many experimental studies on wave breaking encouraging the development of numerical model as useful tool to assisting in the interpretation and even the discovery of new phenomena. One of the great advantages of the numerical models is their ability to disclose the evolutions of undertow currents and turbulence quantities in the spatial and temporal domains, which are too expensive to be investigated by experiments. Therefore, the main emphasis for research is placed on the application and development of numerical methods. Furthermore, for consistent and accurate results, it is essential to calibrate the numerical models with experimental data.
The numerical models can be classified as Eulerian or Lagrangian method. In Eulerian method, the space is discretized into a grid or mesh and the unknown values are defined at the fix points, while a Lagrangian method tracks the pathway of each moving mass point. The Eulerian methods such as the finite difference methods (FDM), finite volume methods (FVM) and the finite element methods (FEM) have been widely applied in many fields of engineering because are very useful to solve differential or partial differential equations (PDEs) that govern the concerned physical phenomena [19, 20, 21, 22, 23]. Despite the great success, grid based numerical methods suffer from difficulties in some aspects such as the use of grid/mesh makes the treatment of discontinuities (e.g., wave breaking, cracking and contact/separation) difficult because the path of discontinuities may not coincide with the mesh lines.
Therefore, during the last years, research has been focused on Lagrangian techniques such as Discrete Element Method (DEM) [24], Smoothed Particle Hydrodynamics (SPH) [25], Immersed Particle Method (IPM) [26, 27]. The development and applications of the major existing Lagrangian methods have been addressed in some review articles such as [28, 29, 30]. In general, the Lagrangian methods provide accurate and stable numerical solutions for integral equations or partial differential equations (PDEs) with all kinds of possible boundary conditions using a set of arbitrarily distributed nodes or particles. During the last years, Smoothed Particle Hydrodynamics (SPH) has become a very powerful method for CFD problems governed by the Navier–Stokes equations such as fluid-dynamic problems with highly non-linear deformation [31, 32, 33, 34, 35, 36, 37]; multi-phase flows for coastal and other hydraulic applications with air-water mixture sand sediment scouring [38, 39, 40, 41, 42]; oscillating jets inducing breaking waves [43] and nonbuoyant jets in a wave environment [44, 45, 46]; fluid/structure/soil-interaction [47, 48, 49]; hydraulic jumps [50, 51, 52, 53]; multi-phase flows and oil spill [54, 55].
The present chapter is organized as follows. First, an WCSPH method is developed using the RANS equations and a two-equation
A Lagrangian numerical model is developed to solve free surface turbulent flows. The flow field is governed by the Reynolds Averaged Navier–Stokes (RANS) equations and the
Using the SPH approach, the fluid flow domain is initially represented by a finite number of particles. These particles can be viewed as moving numerical nodes, which move according to the governing equations and boundary conditions. Each discrete point is associated to an elementary fluid volume (or particle) i, which has position xi and constant mass mi.
To find the value of
where
Dehnen and Aly [60] showed that the Wendland
The advantage of SPH approach is that differential operator applied to
For further details on the different methods for SPH approximations of all the vector operators, the reader can see [67, 68]. In a Lagrangian frame, the Reynolds-averaged Navier–Stokes (RANS) equations and the
where
The RANS equations (3) in the SPH semi-discrete form become
where
The momentum equation is then solved to yield an intermediate velocity field
using a velocity smoothing coefficient
A pressure smoothing procedure is also applied to the difference between the local and the hydrostatic pressure values [71] in order to reduce the numerical noise in pressure evaluation which is present, in particular in WCSPH, owing to high frequency acoustic signals [72]. The present method is applied only to the difference between the intermediate pressure field
The eddy viscosity coefficient
where
According to Eq. (6) both the production term and dissipation term for ε become singular when
The results obtained from the numerical model outlined in the previous section have been validated against extensive experimental data [14], and then used to obtain further insight in the physics of the flow here analyzed.
The detailed experimental setup has been given in De Serio and Mossa [14]. Here only some important parameters are summarized.
Experiment was carried out in the wave flume 45 m long and 1 m wide of the
Figure 1a–f show the different parts of the complex experimental apparatus, which comprises the LDA system, the resistance wave gauge system and the wavemaker system. Further details about the experimental tests can be found in [14].
Experimental apparatus: (a) LDA probe; (b) DANTEC FVA signal processor and process computer; (c) laser coherent Innova and Dantec 2D fiber flow optics; (d) process computer with a AD/DA board for the wavemaker control; (e) a part of the wave channel; (f) the wavemaker.
A sketch view of the experimental setup is shown in Figure 2.
Sketch view of experimental setup.
Table 1 shows the main parameters of the examined waves listed for each experiment, such as the offshore wave height
Breaking type | ||||||
---|---|---|---|---|---|---|
T1 | 11 | 2 | 4.62 | 0.70 | 0.37 | Spilling/plunging |
T2 | 6.5 | 4 | 10.12 | 0.70 | 0.74 | Plunging |
Experimental parameters of the analyzed regular waves.
in which
Water surface elevations and velocities were measured at six different sections along the longitudinal axis of symmetry of the wave channel named 76, 55, 49, 48, 47, 46 and 45 (see Table 2). Specifically, for all two tests, section 48 was in the pre-breaking region, section 47 was where the incipient breaking occurred, while in sections 46 and 45, the wave re-arranged into a bore.
Investigated section | Distance from paddles (m) | |
---|---|---|
Section 76 | 10.56 | 70.0 |
Section 55 | 19.80 | 31.0 |
Section 49 | 22.44 | 16.5 |
Section 48 | 22.88 | 14.0 |
Section 47 | 23.32 | 11.3 |
Section 45 | 24.20 | 8.5 |
Location of measurement sections.
The WCSPH method coupled with a
Sketch of the computational domain wave channel with location of the seven investigated sections, used to calibrate the numerical model.
The adopted offshore boundary condition guarantees a regular development of the wave train before the sloping section of the channel and, therefore, does not influence the quality of the numerical solution, as shown by [32].
For both the two tests, the offshore boundary condition has been treated as dynamic boundary condition modeled by a numerical wave paddle also composed of ghost particles whose motion has been forced to obtain the frequency and amplitude of the wave paddle needed to generate the desired sinusoidal wave [76]. The initial water depth was set equal to 0.70 m. In the present simulations, the initial particle spacing Σ = 0.022 m, the value of η/Σ = 1.5 and
The instantaneous SPH particle distribution and velocity magnitude snapshots of the breaking wave are shown in Figures 4a–e and 5a–e, respectively, for the spilling and plunging breakers. These results show that the general features of wave breaking, collapsing and a turbulent bore propagating have been well captured by the SPH computations.
Instantaneous SPH velocity field in the SPH simulation of spilling wave (T1): (a) before; (b)–(c) during and (d)–(e) after breaking.
Instantaneous SPH velocity field in the SPH simulation of plunging wave (T2): (a) before; (b)–(c) during and (d)–(e) after breaking.
In order to further verify the accuracy of the SPH model the time series of wave elevations, horizontal and vertical velocities at the investigated sections (Figure 3) have been compared with the experimental data of De Serio and Mossa [14]. As an example, in Figure 6a–c both laboratory and numerical wave surface elevations, and velocities at vertical sections 48 and 45 are plotted for T1, referring to the point located at 1 cm from the bottom. The agreement between the calibrated numerical results and the laboratory measurements is fairly good.
Instantaneous computed and measured (a) wave elevations, (b) horizontal and (c) vertical velocities in section 48 and section 45 for T1.
One of the great advantages of the numerical models is their ability to show the evolutions of vorticity and turbulence quantities in the spatial and temporal domains, which are too expensive to be investigated by experiments. Using the SPH computational results, the turbulent kinetic energy distributions are shown in Figures 7a–e and 8a–e, respectively, for the spilling (T1) and plunging (T2) waves. For both breakers, the turbulence quantity has the largest values near the free surface and decreases into the water column. However, the results highlight that there exist fundamental differences in the dynamics of turbulence between the spilling and plunging breakers, which can be related to the processes of wave breaking production.
Instantaneous turbulence intensity distributions in the SPH simulation of spilling wave (T1): (a) before; (b)–(c) during and (d)–(e) after breaking.
Instantaneous turbulence intensity distributions in the SPH simulation of plunging wave (T2): (a) before; (b)–(c) during and (d)–(e) after breaking.
For the spilling wave (T1), higher turbulence levels are mainly concentrated in the breaking wave front and the highest turbulence level appears in the roller region (Figure 7d). After the breaking, as the wave propagates forward, the turbulence kinetic energy decreases (Figure 7e). Instead, the turbulence levels increase rapidly after the wave breaking for the plunging case (T2) as shown in Figure 8c–e. The maximum turbulence level is generated as the plunging jet touches down on the wave trough (Figure 8d) in sections 46–45 (Figure 2); After the breaking, the roller continues to spread downwards and therefore high turbulence levels are generated beneath the free surface after breaking (Figure 8e).
Using the SPH computational results, the vorticity maps are shown in Figures 9a–e and 10a–e, respectively, for the spilling and plunging waves. Vorticity is defined as
Instantaneous values of ω distributions in the SPH simulation of spilling wave (T1): (a) before; (b)–(c) during and (d)–(e) after breaking.
Instantaneous values of ω distributions in the SPH simulation of spilling wave (T2): (a) before; (b)–(c) during and (d)–(e) after breaking.
and is computed using instantaneous values of the horizontal and vertical velocity.
As noted by several authors [77, 78], for both breakers (T1 and T2), when the breaking begins, positive vorticity occupies the whole region of the surface roller and spreads out over the whole water column. However, the vorticity levels increase rapidly after the wave breaking for the plunging case (T2) due to the strong impingement of the jet on the forward trough, inducing a propagation of the positive vorticity towards the bottom (Figure 10c–e).
Moreover, the results highlight that there exist differences in the dynamics of vorticity between the spilling and plunging breakers. In fact, only during spilling formation (T1), small structures of negative vorticity are generated, instead when the plunging breaker (T2) occurs the fluid is relatively free of negative vorticity regions.
Figures 11 and 12 show the comparison between the instantaneous map of vorticity and of the surface parallel convective acceleration for the spilling and plunging waves (T1 and T2) when the breaking begins at time step of Figures 9b and 10b, respectively. The surface parallel convective acceleration here has been computed following [79]. As noted by Dabiry and Gharib [80], for both breakers (T1 and T2), a flow deceleration (Figures 11b and 12b) occurs in the same location where peaks of positive vorticity appear (Figures 11a and 12a). Therefore, the present results confirm the findings by Dabiri and Gharib [80] that the vorticity is convected due to the sharp velocity gradient of the fluid near the free surface with respect to the fluid below.
SPH simulation of spilling wave (T1): (a) Vorticity map and (b) surface-parallel convective acceleration map.
SPH simulation of plunging wave (T2): (a) Vorticity map and (b) surface-parallel convective acceleration map.
In the present chapter a WCSPH method has been developed using the RANS equations and a two-equation
For the spilling wave (T1), during the pre-breaking and breaking stages, the maximum turbulence intensity has been generated near the free surface and decreases as the vortex moves downstream. Instead, the turbulence levels increased rapidly after the wave breaking for the plunging case (T2). In fact, the maximum turbulence level was generated as the plunging jet touches down on the wave trough and after the breaking, the roller continued to spread downwards and therefore high turbulence levels were generated beneath the free surface after breaking.
For both breakers (T1 and T2), analyzing the instantaneous vorticity distributions, when the breaking begins, positive vorticity has occupied the whole region of the surface roller and has spread out over the whole water column. However, only during spilling formation (T1), small structures of negative vorticity have been generated, instead when the plunging breaker (T2) occurs the fluid was relatively free of negative vorticity regions.
Furthermore, comparing the instantaneous map of vorticity and of the surface parallel convective acceleration for the spilling and plunging waves (T1 and T2), the present results confirmed the findings by Dabiri and Gharib [80] that the vorticity was convected due to the sharp velocity gradient of the fluid near the free surface with respect to the fluid below.
Agricultural, urban, and natural tree stands have been the focus of extensive plant pathogen diagnostic and disease management research in recent decades [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16] which recorded an increase in the number of new fungal and bacterial pathogens and their detrimental impact on agroecosystems, ecosystems, and the human society. The economic effects of these pathogens are reflected in lost fresh fruit produce [17, 18, 19], reduced yields and quality of fruit or wood and cork products [20, 21], diminished ecological tree services, and death of whole trees, stands, and forest regions or decimation of fruit industries [19, 22].
\nIf left unmanaged, apple scab fungus
The biology of majority of these microorganisms, excluding
Tree injection, often referred to as trunk or stem injection, is a method of target precise delivery or application of pesticides, plant resistance activators and fertilizers into the xylem vascular tissue of a tree with the aim to protect trees from insect pests and pathogens or to provide tree nutrition and/or correction of micronutrient deficiencies. It primarily harnesses the transport capacity of the tree’s vascular system driven by transpiration stream of water in these tissues to translocate and distribute the active compounds into the trunk, branches, canopy and roots where protection or nutrition is needed. Tree injection as a plant protection method is viewed as environmentally safer alternative for pesticide application because it secures significant reduction of non-target exposure of water, soil, air and wildlife to pesticides and fertilizers in landscapes and urban greening areas. The active ingredients are delivered within the tree, thus providing selective exposure to plant pests, with limited negative effect of weather conditions like rain or sun radiation on the injected compound and with creating no immediate pesticide residue losses outside the tree.
\nTrunk injection relies significantly on tree physiology processes related to water transport, xylem and phloem tissue functions, and the weather conditions that influence these specific plant processes. To achieve delivery of an effective pesticide dose, its distribution and expected management of plant detrimental organism or nutrient deficiency, there are several key factors which should be monitored by an applicator as they influence success of trunk injection for these purposes. Besides the plant pathogen biology, ecology, and epidemiology, several factors play a key role in success of trunk injection efficacy: the time of application in relation to detrimental organism establishment and symptom occurrence [11], the season and time of the day of application [41], the chemical properties of pesticide active ingredient and its formulation [42], the injected volume or dose of a pesticide, and the type of tree injection device or technology. For example, a more effective management of plant disease or insect infestation can be achieved by the preventive injections of pesticides in comparison to the therapeutic pesticide applications after the disease or infestation has already occurred. Tree injection of active compounds is much faster and easier during spring and early summer months in comparison to the late or mid-summer, late fall and winter, because water in the soil is abundant and the green leaf canopy is facilitating intensive transpiration pull and flow of water through the xylem tissue in hardwood trees, starting from the roots and branches to the leaves [41]. The three key properties of injected active ingredient that determine its mobility or binding in xylem of the tree are organic carbon-water partitioning coefficient (ml/g or μg/g) or carbon adsorption coefficient (Ko/c), water solubility, and formulation type. Ko/c expresses the level of adhesion or adsorption of pesticide active ingredient to the carbon rich compounds in certain environments such as soil or xylem and is defined as a ratio of mass of a chemical that is adsorbed in a certain environment per unit mass of organic carbon in that environment per the equilibrium chemical concentration in a solution. Active ingredients that have high Ko/c values will strongly bind to the organic compounds present in soil, sap or xylem and reduce their systemic movement i.e. translocation, accumulation and distribution in the canopy, thus reducing the efficacy in pathogen or pest control. In contrast, the ingredients with low or moderate Ko/c values move and distribute fast after tree injection and distribute well in the canopy, securing good pest or pathogen control. Pesticide formulation is a form of a pesticide active ingredient ready for use or which quite often requires dilution in water prior to application. Formulation is made by adding different inactive ingredients with the aim to improve the properties of an active ingredient such as solubility, surface adhesion, distribution, effectiveness, shelf life, stability, handling or application (e.g. solvents, emulsifiers, surfactants and other adjuvants). Formulation of a pesticide or a fertilizer determines the properties and residue stability of an active ingredient and can modulate its mobility in xylem of phloem after tree injection for pest or plant management [12, 42]. Finally, trunk injection devices can loosely be divided into the ones using drill- or needle-based technology [43]. In case of the first one, access to xylem for pesticide delivery device is enabled by drilling into the trunk or root flare wood, removing a small part of the wood, and sealing of the opened injection port with an inserted plastic plug or not (plug contains an injection valve with a one-way silicone septum) [44]. For the injection application to be faster and hence economical in urban tree care, this system uses compressed air or hydraulic pressure to force-inject the pesticide solution into the wood. The second technology uses a knife-like or a flat, screwdriver-like needle with a lenticular profile, which is inserted into the wood by a hammer thus separating the wood fibers and creating a crevice while the delivery of a pesticide solution is conducted through the needle and infused into the wood [45]. This system can use force of hydraulic or compressed air pressure to deliver the pesticide solution into the xylem or is solely relying on the Venturi effect (vacuum) created by a transpiration stream in xylem to infuse the pesticide solution into the wood [45, 46, 47]. This injection technology requires longer time for injection solution delivery, especially when transpiration is limited, and thus is often less economical in urban tree care.
\nTree injection was initially developed for pesticide and fertilizer application on large size trees in proximity of urban areas where ground- and air-spray applications are impractical due to substantial pesticide losses through drift, lack of proper canopy coverage, or are prohibited due to possible human and domestic animal exposure. The second driver for development of tree injection and its more frequent use in recent decades has been the destructive nature and an increasing need for effective management options for invasive tree pathogens like
Due to a demonstrated ability of single trunk injection to increase the efficacy of injected pesticides over multiple years, a possibility to reduce the of number of topical spray applications [10, 12, 48] and a rising incidence of woody plant pathogens and insect pests in the environment [31, 33, 49, 50, 51], this approach has recently been investigated in agriculture where topical pesticide applications for plant food production is intensive. The most investigated tree fruit crops and their pathogens and insect pests are citrus (e.g.
While trunk injection for pesticide delivery is a relatively new technology investigated in tree-based agriculture for managing diseases like citrus greening [14, 15, 16, 66, 67, 68] or fire blight [11, 44], research in agricultural engineering will first need to design or invent an application system/s that allow scalability, i.e. achieving simultaneous trunk injection of large number of trees in a short period of time. Besides this end goal many other key questions arising from research outlined above will need to be addressed through experimental work before tree injection is used in agriculture, even in limited fashion. The first steps are providing enough evidence i.e. providing proof of concept that injected pesticides are effective in tree pathogen and insect management and that injected materials have minimal negative effect on fresh fruit consumer and beneficial orchard fauna. Because effective management options for Bot canker and decline of different
In the binomial nomenclature of fungi, Bot canker pathogen
Because
Majority of evidence indicates that
The sexual stage of an ascomycete fungus
Depending on the substrate it colonizes over the year, the life cycle of apple scab fungus has the saprophytic and the parasitic phase in its development. The saprophytic phase starts with apple leaf drop in autumn.
During the time after spores of apple scab fungus land on the susceptible plant surface, while they germinate, and up to 72 h after they penetrate below the cuticle on green tissue, they are vulnerable to spray-applied contact and systemic fungicides, respectively. However, once the infection is established by formation of mycelium under the cuticle, almost all fungicides applied to green plant surfaces have no efficacy in eradicating these infections and eventually lesions with conidia, or their effect is minimal. Furthermore, continued post-infection scab management with spray applications of fungicides that aim to prevent new infections on green tissues is complicated by large populations of conidia, which if exposed to fungicides with specific modes of action might increases the potential for fungicide resistance selection in this devastating pathogen. Because of the specific lifestyle of
Fire blight is caused by a Gram-negative bacterium
Once
During the incubation, i.e. usually sometime around 10 – 14 days before the first conspicuous blossom or shoot blight symptoms are visible, small white, amber or orange droplets of bacterial ooze can emerge and drip from the infected green tissues (flower pedicels, floral cup, sepals, immature fruit and shoots). With more wetting events and insect activity, ooze can spread to new flowers and actively growing shoots across the whole orchard. Since blossom and shoot blight symptoms are not yet visible, this dissemination of ooze allows secondary infections and can propel a fire blight outbreak into an epidemic, especially if the antibiotic spray application/s were not conducted during bloom. Once incubation is over, blossom blight is visible as dead, black or brown flower clusters with more droplets of bacterial ooze developing if weather conditions are humid. Shoot blight and immature fruit infections are visible as black or brown “flags” or “strikes” and brown to black shriveled fruitlets, respectively. Blighted shoot tips often bend in the typical shape of Shepherd’s crook. Fire blight cankers on branches, trunk and rootstock are formed by pathogen’s progress via xylem or the cortical parenchyma from the established infections on flowers, shoots and suckers, into the wood bark tissues.
\nWhen
To test the effect of injected fungicides and activators of plant systemic acquired resistance (SAR) [11] for reduction of Bot canker caused by
Trunk injected fungicide treatments evaluated for management of Bot canker fungus Diplodia corticola on northern red oak trees, Quercus rubra.
One injection point i.e. port per trunk of each potted tree, positioned ca. 5–7 cm above the ground level, was created by drilling 7–10 mm into the xylem tissue with a 4.3 mm diameter drill attached to a cordless drill. To inject the protective liquid solutions listed in Table 1, we used a Stinger needle for plugless trunk injection assembled on an individual feed line attached to the Tree IV air/hydraulic microinjection system, which operated at 60 psi air pressure (Arborjet Inc., Woburn, MA). The Stinger needles are used for injection of trees when trunk injection ports of large diameter (9.5 mm) are of concern or should be avoided and for injection of trunks with small diameters. The diameter of injection port for inserting a Stinger needle is smaller and does not require sealing with an Arborplug. In year one, the injected potted oak trees had trunk diameter at 5 cm height averaging 1.3 cm and ranging from 1 to 2.1 cm. In year two, a new set of injected trees had the diameter at 5 cm height averaging 1.5 cm and ranging from 1.1 to 2.2 cm. Trunk injection were conducted on 12 June in year one and on 16 August in year two.
\nTrees were inoculated with
Statistical analysis was done with MIXED procedure in SAS Studio software (SAS Institute Inc. 2017, Cary, NC) using the xylem necrosis areas (cm2). If the fungicide effect was found to be statistically significant (
With the goal to optimize timing and number of fungicide injections for management of apple scab fungus
Fungicide treatments trunk-injected across two seasons and sprayed for management of apple scab fungus Venturia inaequalis on ‘Mac Spur’ apple trees.
On each trunk injection date with fungicides listed in Table 2, a separate set of four cardinally-oriented trunk injection ports per each tree of ‘Mac Spur’ was created by drilling 25 mm into the xylem with a 9.5 mm diameter drill bit attached to a cordless drill. The first set of four injection ports was positioned ca. 25 cm above the ground level. The subsequent sets of four injection port were positioned ca. 5 cm above and between the lower four-port sets. Every port was sealed with Arborplug no. 4 (Arborjet Inc., Woburn, MA) positioned just below the bark level to allow port closure with cambium callus [44]. To inject the fungicides, we used the Quik-jet microinjection system (Arborjet Inc.) operating at hand-generated hydraulic pressure to deliver low volumes of liquid for injection, thus allowing faster application times, and the Tree IV air/hydraulic microinjection system (Arborjet Inc.) operating at up to 60 psi of air pressure to deliver large solution volumes of liquid for injection (≥600 ml). In the experiment 1 (Table 2), we injected all the treatments listed for 15 October in year one with Quik-jet (Table 2). On 11 April in year two, we injected propiconazole using the Viper air/hydraulic microinjection system set at 90 psi air pressure (Arborjet Inc.). At the later dates, we injected Alamo using the Tree IV and Phosphojet using the Quik-jet. In the experiment 2, we injected Phosphojet with Quik-jet and cyprodinil + difenoconazole with Tree IV. The needle/s of each of the used injection devices was inserted through the Arborplugs allowing the total liquid volume per tree, at one injection time, to be divided and delivered equally among the four ports.
\nAll the experiments were conducted under naturally high infection pressure during the primary season of
To test the effect of injected bactericides and activators of plant systemic acquired resistance (SAR) for blossom and shoot blight incidence reduction, the orchard experiments were conducted over 2 years (Table 3). The early spring injections in year one (26 March) were conducted with Viper air/hydraulic micro-injection system® at under 110 psi of air pressure and late spring injections (23 April) were done with Tree IV® air/hydraulic micro-injection system, at 60 psi air pressure (Arborjet Inc., Woburn, MA). In the year two, trunk injections on 1 and 22 May were applied using Tree IV® air/hydraulic micro-injection system at 60 psi of air pressure. The injection needles of these devices were inserted through the one-way valve silicone septum in the Arborplugs® which allowed delivery of protective solutions into he drilled injection ports. In each injection, the total injected volume per tree was divided equally among the four ports (Table 3). Four injection ports per each apple per tree, positioned ca. 10–15 cm above the ground level, were cardinally oriented and created by drilling 25 mm into the xylem tissue using a 9.5 mm diameter drill bit attached to a cordless drill. Each port was sealed with Arborplug® no. 4, by pushing the plug with a specialized screwdriver-like tapper hit with a hammer (Arborjet Inc., Woburn, MA, USA). The plug was positioned just below the bark level to allow port closure with cambium callus.
\nTrunk-injected treatments of bactericides and SAR-activators for management of fire blight bacterium Erwinia amylovora on flowers and shoots of ‘Gala’ and ‘Jonathan’ apple trees.
In the year one, we used 14-year-old ‘Gala’ apple trees which were trunk-injected using the compounds and dosages listed in Table 3. Injections were performed at the tight cluster growth stage in apples (26 March), or 21 days before 80% bloom, and at petal fall growth stage (23 April). In the year two, experiments were conducted on a new set of 21-year-old ‘Gala’ apple trees, injected with the same doses in Table 3. Injections were applied at early tight cluster growth stage (1 May) or 13 days before 80% bloom and at petal fall (22 May). The treatment Actigard 1 was injected only on the first date in both years (Table 3). Each dose in every treatment, except the Phosphojet, was diluted and injected with 520 ml of water per tree. The doses per tree were chosen according to the four rules: (1) the dose was equivalent to the US EPA pesticide label rate for a maximum amount per 0.405 ha with 250 planted apple trees; (2) the dose was one half of the maximum US EPA label rate allowed per one season; (3) the dose was equal to a rate delivered in one spray application treatment per 0.405 ha with 250 apple trees; or (4) the dose was selected based on previous research with trunk injection of similar pesticides [116]. Trees injected with water and the non-injected non-inoculated trees served as negative controls for efficacy comparisons. In year one, each treatment was replicated on four trees arranged in a randomized complete block design, where blocking controlled the variable crown tree sizes (large, medium, medium-small, and small) [117]. In the year two, we used the same number of replicate trees per treatment but arranged in a completely randomized design (CRD).
\nIn year one, on 16 April at 80% bloom, apple flowers of all experimental trees were inoculated with a suspension of
We analyzed the data with MIXED procedure in SAS 9.3 (SAS Institute, 2012). The main effect of treatments on blossom and shoot blight incidence were analyzed using
To test the reduction of shoot blight severity with bactericide oxytetracycline hydrochloride (Arborbiotic, MFG Scientific Inc., EPA Reg. No 88482-1; Arbor-OTC® Injectable Tree Antibiotic, Arborjet Inc., Reg No. 74578-7), apple trunk injections were performed in a similar fashion described above, but by using a Quik-jet® micro-injection system instead (Arborjet Inc., Woburn, MA, USA). This device relies solely on hand-generated hydraulic pressure to inject the necessary pesticide solution volume in each port. The injection ports were created and sealed with Arborplugs (Arborjet Inc.) in the same way as described above and injected volume per tree was divided equally among the four ports. The experiments were conducted in 2 years. In year one, at petal fall growth stage (23 April) mature 12-year-old apple trees of cv. ‘Jonathan’ were trunk-injected with Arborbiotic using dose in Table 3 diluted at 10% in water. The total dose per tree was calculated based on the unique trunk diameters at 30 cm height using the EPA label instructions. In year two, the same apple trees injected in year one were re-injected at petal fall (22 May) using the same dose in Table 3 delivered via a fresh set of drilled injection ports above the previous year’s set of injection ports. In both years, Arborbiotic treatment as well as water control were replicated on four trees arranged in a CRD.
\nA total of 10 terminal shoots per each tree were inoculated on 7 May in year one and on 30 May in year two. We used a previously reported inoculation method [114]. In brief, the upper third of leaf blade of the second or the third youngest leaf on each shoot tip was cut perpendicular to the leaf midvein with scissors dipped in
We analyzed the data using MIXED procedure in SAS 9.3 (SAS Institute, 2012). If the main effect of treatment on shoot blight severity was found significant (
All the three fungicides trunk-injected preventively provided significant reduction of Bot canker caused by
Percent reduction i.e. control of Bot canker necrosis area in trunk xylem in relation to water control on Quercus rubra trees in year one (A) and year two (B) achieved with trunk injections of fungicides Propizol (propiconazole), Arbotect (thiabendazole) and Phosphojet (potassium phosphites). Means followed by different letters are significantly different (A: p < 0.1, LSD test; B: p < 0.05, LSD test). In year one (A), the area of Bot canker necrosis in trunk xylem in water control was 5.15 cm2 and in year two (B) 5.8 cm2. Each mean consists of six replicate trees.
In the experiment 1, fungicides injected four times in total, once in fall and then three additional times in spring, during the primary scab infection period, provided significant reduction of apple scab incidence on spur and shoot leaves (Figure 2A). On spur leaves, the best scab reduction of 45.5% was achieved with injected Phosphojet high, but this control was not better in comparison to 78.6% in spray standard applied in spring during the primary scab season (Figure 2A). In contrast, control with injected Phosphojet high on shoots outperformed the spray standard with 73.6 vs. 62.9% in scab reduction (Figure 2A). Similarly, Alamo performed better on shoot leaved than on spur leaves (Figure 2A).
\nPercent reduction i.e. control of apple scab in relation to water control on ‘Mac Spur’ trees after in experiment 1 (A) and experiment 2 (B) achieved with trunk injections and sprays of potassium phosphites (Phosphojet, Arborfos) and of difenoconazole + cyprodinil (Inspire Super). Means within each graph section i.e. apple organ followed by different letters are significantly different (t-test, p < 0.05). F - one fall injection; 3S - three spring injections; S - one spring injection. In experiment 1 (A), scab incidences in water control on spur and shoot leaves were 72.2 and 54%. In experiment 2 (B) scab incidences in water control on spur leaves, shoot leaves and fruit were 88.3, 94.4 and 95.5%, respectively. Each mean consists of six replicate trees.
In the experiment 2, fungicides injected 1–2 times in total, across or within two seasons of fall and spring, revealed that the injected Inspire Super treatments largely did not significantly reduce disease incidence on spur and shoot leaves when compared to the water control. In contrast, all the injected Phosphojet treatments and Agrifos sprays did. Comparisons among these treatments clearly demonstrated that on all the three rated apple organs (Figure 2B), Phosphojet trunk injections provided statistically better apple scab reduction i.e. control in comparison to all the Inspire Super trunk injections. On spur leaves, two Phosphojet trunk injections, fall plus spring, was the best treatment among injections by providing 46.3% control which was similar to the Inspire Super sprays (Figure 2B). On shoot leaves, two Phosphojet trunk injections both done in spring, provided the best scab control of 66.5% similar to nine sprays of Agrifos (Figure 2B). On fruit, scab control was the best in Phosphojet trunk injection done once or twice in spring, and in fall plus spring: 62.8, 69.7 and 64.6%, significantly outperforming both the Agrifos and the Inspire Super sprays (Figure 2B).
\nIn both year one and year two, all the trunk-injected bactericides (Agrimycin) and SAR-activators (Actigard, Phosphojet) provided significant reduction of blossom blight incidence in comparison to the water control (Figure 3). In year one, which had low disease pressure (Figure 3A), there was no significant difference among all the treatments in disease reduction i.e. control (37.9–61.1%). In year two, with high infection pressure, Agrimycin was the best providing 28.9% blossom blight control (Figure 3B). Averaged across both years, Agrimycin and then Phosphojet were the best treatments with 45 and 40.5% achieved control, respectively (Figure 3).
\nPercent reduction i.e. control of blossom blight incidence in relation to water control on ‘Gala’ apple trees in year one (A) and year two (B) achieved with one to two trunk injections of ‘Gala’ apple trees with Agrimycin (streptomycin), Phosphojet (potassium phosphites) and Actigard (acibenzolar-S-methyl). Means within each graph followed by different letters are significantly different (t-test, p < 0.05). Blossom blight incidence in water control in year one was 47.2% (A) and in year two 72.9% (B). Each mean consists of four replicate trees averaged across three time points when disease was rated.
In year one, none of the trunk-injected products provided significant reduction of shoot blight incidence in comparison to the water control, hence did not differ among each other (Figure 4A). In year two, under high disease pressure, all the injected products significantly reduced shoot blight incidence for 23.4–36.5% in comparison to the water control, but when compared they did not significantly differ between each other (Figure 4B). If averaged across both years, Agrimycin and then Phosphojet achieved the best control of 53.5 and 42.8%, respectively (Figure 4).
\nPercent reduction i.e. control of shoot blight incidence in relation to water control on ‘Gala’ apple trees in year one (A) and year two (B) achieved with one to two trunk injections of Agrimycin (streptomycin), Phosphojet (potassium phosphites) and Actigard (acibenzolar-S-methyl). (A) In year one, the injected treatments did not significantly reduce shoot blight incidence relative to water control. (B) Means followed by different letters are significantly different (t-test, p < 0.05). Soot blight incidence in water control in year one was 22.4% (A) and in year two 68.5% (B). Each mean consists of 4 replicate trees averaged across two time points in (A) and three time points in (B) when disease was rated.
In both years Arborbiotic provided significant reduction i.e. control of shoot blight severity in comparison to the water control (Figure 5). When averaged across both years, the control of shoot blight severity reached 72.4% (Figure 5).
\nPercent reduction i.e. control of shoot blight severity relative to water control achieved from a single trunk injection of ‘Jonathan’ apple trees with Arborbiotic (oxytetracycline hydrochloride) in each year. Means with an asterisk indicate significant reduction of shoot blight severity (year one: Tukey’s HSD test; year two: t-test, p < 0.05). Each mean consists of four replicate trees averaged across five time points in year 1 and six time points in year 2 when disease was rated.
We present the first data on management of
The organic carbon-water partitioning coefficient (Ko/c) for thiabendazole is moderate to high and ranges from 1104 to 4680 ml/g, while water solubility is 50 mg/L at pH 7 and 38 mg/ml at pH 2 [118]. These parameters indicate on low to no mobility of thianbendazole in xylem as a carbon rich environment. The Ko/c of propiconazole is 1086–1817 ml/g which is moderate to high [119, 120] and water solubility is low, 100–150 mg/L [121]. This could have contributed to slow and reduced uniformity in distribution of injected fungicides in xylem. However, both Arbotect and Propizol are fungicides formulated for trunk injection on hardwood trees and if properly diluted and delivered preventively they can accumulate sufficiently to secure the internal control of specific plant diseases (e.g. Dutch elm disease caused by
In the future studies, we predict that the efficacy of preventive fungicide applications against
We evaluated the similar fungicides on apple,
The efficacy against this subcuticular pathogen that infects just below the waxy layer on leaves and fruit, clearly depended on the apple canopy organ and the time/s of fungicide injection/s. Namely, on spurs which hold much fewer leaves in total in comparison to the shoots, the best leaf scab incidence reduction was 45.5 and 46.3%. In contrast, scab reduction on shoot leaves with Phosphojet reached 66.5 and 73.6%. On apple fruit, scab reduction reached up to 62.8, 64.6 and 69.7%. These efficacy patterns clearly demonstrate the differential influences of the tree’s yearly and organ-specific physiology, the properties of injected compound, and the injection timing on the accumulation of fungicides in the canopy. Since the major water transport in xylem, occurs in spring, at least one to two injections of phosphites in early spring gave a good disease control, depending on the canopy organ. The best scab control with injected phosphites was achieved on the shoot leaves, followed by apple fruit, and then on the spur leaves. The injected phosphites probably accumulated more in the shoot leaves than in the spur leaves and they accumulate more in fruit than in spur leaves. This can be explained by the variable rates of transpiration from these organs, which influences the speed and abundance of fungicide accumulation after trunk injection. The total leaf area on shoots is larger in comparison to spurs. The fewer leaves on spurs, which are first to develop in spring and early reach their full size, have fewer total number of stomata on them in comparison to more numerous shoot leaves. Additionally, from petal fall up until terminal bud set, shoots keep growing and developing more leaves on the tips. Hence, apple shoots hold the higher number of stomata in total, thus allowing much higher transpiration intensity, abundant accumulation of injected fungicides and thus scab control. Similarly, apple scab control was lower on fruit than on shoots which could be explained by the fact that apple fruit hold 10- to 100-fold lower frequency of stomata on their epidermis in comparison to the apple leaves [122].
\nThe chemical properties of different active ingredients impact their distribution and accumulation in the canopy. For example, potassium phosphites have higher water solubility of 500 g/L in comparison to propiconazole and difenoconazole which have low to very low water solubilities of 100–150 mg/L and 13 mg/L, respectively [121, 123]. Potassium phosphites have low organic carbon-water partitioning coefficient (Ko/c) from 228 to 587 ml/g in comparison to moderate to high of propiconazole, 1086–1817 ml/g, and of difenoconazole, 3870–11,202 ml/g, respectively [119, 120]. This difference likely allowed phosphites to move faster in xylem [124] and accumulate more in leaves and fruit than the other injected fungicides. At the same time, propiconazole and difenoconazole were probably bound to the organic phase of xylem symplast and apoplast, thus lowering their accumulation in leaves and fruit and reducing their effect on scab incidence [65]. This is often referred to as a reservoir effect and Ko/c as is an important property of a pesticide that can explains its limited or abundant accumulation in the canopy [65, 125]. Besides the Ko/c and water solubility, the inactive components of the Inspire Super pesticide formulation we injected (stickers, emulsifiers, surfactants, etc.) could reduce the abundant accumulation of difenoconazole and a better scab control. Fungicides have to be formulated for injection to secure their upward translocation in xylem and often diluted prior to trunk injection to reduce the impact of Ko/c effect. Once the high solubility, low Ko/c and injectable formulation are possible for one active ingredient, a rapid and desired control effect on plant pathogen or insect pest can be expected [42, 45, 126].
\nThe reduction of apple scab and our prior work on analyzing the residues of injected pesticides on apple leaves and fruit [12, 61] indicates that accumulation of trunk-injected fungicides in the wood and canopy is a time-demanding process chiefly shaped by the tree physiology and tissue resistance points [127, 128]. Trunk injection is an opposite process to the immediate deposition of fungicide solution on the tree canopy by foliar spray applications. However, even though the injected dose per tree of phosphites in Phosphojet was 1.6–2 times higher than in the Agrifos sprays, the fact that just two injections secured better control of scab on fruit and spur leaves in comparison to nine Agrifos sprays demonstrated better persistence of injected Phosphojet. This shows that trunk injection is a superior delivery method for phosphites as it enhances their activity for 1–2 growing seasons [12].
\nThe fire blight bacterium
The best control i.e. reduction of blossom blight incidence across both trial years was achieved with two trunk injections of Agrimycin (45%) and of Phosphojet (40.5%). However, under high and low infection pressures in the two trial years, the levels of control with these materials (28.9, 61.1%, 25.1, 55.9) were far from comparable to 92–99% control often achieved and expected with preventive flower spray application of Agrimycin and Kasugamycin in commercial apple orchards [129, 130]. In the case of injected Phosphojet and Actigard, the achieved blossom blight reduction probably originated from an SAR effect triggered in the nearby spur leaves by these compounds, as the SAR effect in flowers was inconsistent [11]. SAR is a defense plant response which is activated after localized plant exposure to a pathogen or after a spray applications of a synthetic or natural compound, known as an SAR-inducer or activator [131]. Our 1–2 trunk injections of Actigard reduced blossom blight incidence for only 19–42%, indicating that this delivery method cannot not improve the SAR-effect of Actigard on flowers to combat blossom blight successfully. Namely, different sources report from 3 to 91% of blossom blight control with foliar sprays of Actigard on other apple cultivars [132, 133, 134].
\nVegetative flowers parts in
Even though reduction of shoot blight incidence was not statistically significant in year one, which was characterized with low infection pressure, it indicated that trunk-injected Agrimycin and Phosphojet might have potential to perform better than Actigard treatments. However, in year two, under the heavy infection pressure, this was not the case as all the injected treatments were similar. Overall, it seems that the reduction of shoot blight incidence with injected Agrimycin and Phosphojet across both years of 53.5 and 42.8%, was slightly better than the reduction of blossom blight incidence with the same materials of 45 and 40.5%, respectively. Shoots obviously have much higher green tissue area and transpiration rate in comparison to the flowers. Shoots likely accumulate higher amounts of trunk injected compounds in comparison to the green flower parts, which allowed slightly better disease reduction early after injection. Still, the shoot blight incidence reduction was far from the expected control with spray applied antibiotics in commercial apple orchards. In a trial with trunk injection of Arborfos (45.8% mono- and di-potassium salts of phosphorous acid, Mauget Inc., Arcadia, CA, USA), shoot blight was reduced for 67% on inoculated ‘Paulared’ apple trees [116]. The same dose per tree which we delivered in two injections of Phosphojet, achieved shoot blight incidence reduction of 23.4–62.1%. Since we have split the dose delivery temporally, this weakened shoot blight incidence reduction by Phosphojet and probably by Actigard too. In shoot inoculated trials multiple Actigard sprays achieved shoot blight reduction between 2.8 and 50.7% [135, 136] while by trunk injection we achieved only 1.7–30.9% of shoot blight reduction. Hence, the two-time trunk injection does not improve shoot blight reduction by Actigard.
\nThe reduction of shoot blight severity with Arborbiotic (MFG Scientific Inc., USA) was excellent and reached up to 82%. Such an effect with oxytetracycline hydrochloride demonstrates that this active ingredient is readily soluble in water and that the formulation we used is designed for trunk injection. Our results indicated that the trunk injected Arborbiotic limits i.e. stops systemic spread of
Our results on management of three different pathogens with partially similar or different biologies, where
In the biology i.e. life cycle of
In the case of
Finally, there is the case of a complex biology of
The work on
The work on
The fire blight work was funded by USDA-NIFA Pest Management Alternatives grant MICL05066 in 2012 and continuation grant MICL07748 in 2013–2014 to JCW for project “Trunk Injection: A Discriminating Delivery System for Tree Fruit IPM”. The work with evaluating Arborbiotic in reduction of fire blight severity was funded by Arborjet Inc., Woburn, MA, USA.
\nWe thank Joseph J. Doccola, director of research and development at Arborjet Inc. and John J. Aiken, regional technical manager, for donating injection equipment and chemicals used in apple scab and fire blight experiments. For assistance in conducting or facilitating experiments, we thank research staff of at Michigan State University’s Trevor Nichols Research Center (TNRC) in Fennville, MI, Anthony VanWoerkom, Gail Ehret, Jerri Gillett, Jason Seward, Laura Lamb and Kyle Coffindaffer. We acknowledge Dr. Annemiek Schilder, Dr. Brad Day, Dr. Jianjun Hao, Dr. Jim Miller and Dr. Randolph Beaudry for generously sharing their laboratory resources at Michigan State University in support of this research.
\nAll authors declare that the research was conducted without any commercial or financial relationships that could be interpreted as a potential conflict of interest.
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