Open access peer-reviewed Edited Volume

Genetic Manipulation of DNA and Protein

Examples from Current Research

Edited by David Figurski

Columbia University, United States of America

This diverse collection of research articles is united by the enormous power of modern molecular genetics. Every author accomplished two objectives: (1) making the field and the research described accessible to a large audience and (2) explaining fully the genetic tools and approaches that were used in the research. One fact stands out - the importance of a genetic approach to addressing a problem. I encourage you to read several chapters. You will feel the excitement of the scientists, and you will learn about an area of research with which you may not be familiar. Perhaps most importantly, you will understand the genetic approaches; and you will appreciate their importance to the research.

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Genetic Manipulation of DNA and ProteinExamples from Current ResearchEdited by David Figurski

Published: February 5th 2013

DOI: 10.5772/2156

ISBN: 978-953-51-0994-5

eBook (PDF) ISBN: 978-953-51-5352-8

Copyright year: 2013

Books open for chapter submissions

39018 Total Chapter Downloads

8 Crossref Citations

16 Web of Science Citations

28 Dimensions Citations


Open access peer-reviewed

1. Site-Directed Mutagenesis and Yeast Reverse 2-Hybrid-Guided Selections to Investigate the Mechanism of Replication Termination

By Deepak Bastia, S. Zzaman and Bidyut K. Mohanty


Open access peer-reviewed

2. Biochemical Analysis of Halophilic Dehydrogenases Altered by Site-Directed Mutagenesis

By J. Esclapez, M. Camacho, C. Pire and M.J. Bonete


Open access peer-reviewed

3. Targeted Mutagenesis in the Study of the Tight Adherence (tad) Locus of Aggregatibacter actinomycetemcomitans

By David H. Figurski, Daniel H. Fine, Brenda A. Perez-Cheeks, Valerie W. Grosso, Karin E. Kram, Jianyuan Hua, Ke Xu and Jamila Hedhli


Open access peer-reviewed

4. Directed Mutagenesis of Nicotinic Receptors to Investigate Receptor Function

By Jürgen Ludwig, Holger Rabe, Anja Höffle-Maas, Marek Samochocki, Alfred Maelicke and Titus Kaletta


Open access peer-reviewed

5. Site-Directed Mutagenesis as a Tool to Characterize Specificity in Thiol-Based Redox Interactions Between Proteins and Substrates

By Luis Eduardo S. Netto and Marcos Antonio Oliveira


Open access peer-reviewed

6. Protein Engineering in Structure-Function Studies of Viper's Venom Secreted Phospholipases A2

By Toni Petan, Petra Prijatelj Žnidaršič and Jože Pungerčar


Open access peer-reviewed

7. Site-Directed Mutagenesis in the Research of Protein Kinases - The Case of Protein Kinase CK2

By Ewa Sajnaga, Ryszard Szyszka and Konrad Kubiński


Open access peer-reviewed

8. Directed Mutagenesis in Structure Activity Studies of Neurotransmitter Transporters

By Jane E. Carland, Amelia R. Edington, Amanda J. Scopelliti, Renae M. Ryan and Robert J. Vandenberg


Open access peer-reviewed

9. Site-Directed Mutagenesis as a Tool for Unveiling Mechanisms of Bacterial Tellurite Resistance

By José Manuel Pérez-Donoso and Claudio C. Vásquez


Open access peer-reviewed

10. A Mutagenesis Approach for the Study of the Structure-Function Relationship of Human Immunodeficiency Virus Type 1 (HIV-1) Vpr

By Kevin Hadi, Oznur Tastan, Alagarsamy Srinivasan and Velpandi Ayyavoo


Open access peer-reviewed

11. New Insights into the Epithelial Sodium Channel Using Directed Mutagenesis

By Ahmed Chraibi and Stéphane Renauld


Open access peer-reviewed

12. Use of Site-Directed Mutagenesis in the Diagnosis, Prognosis and Treatment of Galactosemia

By M. Tang, K.J. Wierenga and K. Lai


Open access peer-reviewed

13. Inherited Connective Tissue Disorders of Collagens: Lessons from Targeted Mutagenesis

By Christelle Bonod-Bidaud and Florence Ruggiero


Open access peer-reviewed

14. Biological Activity of Insecticidal Toxins: Structural Basis, Site-Directed Mutagenesis and Perspectives

By Silvio Alejandro López-Pazos and Jairo Cerón


Open access peer-reviewed

15. Site-Directed Mutagenesis as Applied to Biocatalysts

By Juanita Yazmin Damián-Almazo and Gloria Saab-Rincón


Open access peer-reviewed

16. Using Cys-Scanning Analysis Data in the Study of Membrane Transport Proteins

By Stathis Frillingos


Open access peer-reviewed

17. Site-Directed Mutagenesis Using Oligonucleotide-Based Recombineering

By Roman G. Gerlach, Kathrin Blank and Thorsten Wille


Open access peer-reviewed

18. Studying Cell Signal Transduction with Biomimetic Point Mutations

By Nathan A. Sieracki and Yulia A. Komarova


Open access peer-reviewed

19. Using Genetic Reporters to Assess Stability and Mutation of the Yeast Mitochondrial Genome

By Shona A. Mookerjee and Elaine A. Sia


Open access peer-reviewed

20. Site-Directed and Random Insertional Mutagenesis in Medically Important Fungi

By Joy Sturtevant


Open access peer-reviewed

21. Recombineering and Conjugation as Tools for Targeted Genomic Cloning

By James W. Wilson, Clayton P. Santiago, Jacquelyn Serfecz and Laura N. Quick


Edited Volume and chapters are indexed in

  • Worldcat
  • OpenAIRE
  • Google Scholar
  • AZ ebsco
  • Base
  • CNKI

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