Open access peer-reviewed chapter

Control of Ribosomal RNA Transcription by Nutrients

By Yuji Tanaka and Makoto Tsuneoka

Submitted: August 1st 2017Reviewed: October 23rd 2017Published: February 28th 2018

DOI: 10.5772/intechopen.71866

Downloaded: 451

Abstract

The ribosome is a unique machine for protein synthesis in organisms. The construction of ribosomes is exceedingly complex and consumes the majority of the cell materials and energy. The materials for ribosome production are supplied by nutrients. Therefore, the production of ribosomes is restricted by environmental nutrients, and cells need mechanisms to control ribosome production in order to reconcile demands for cell activities with available resources. Transcription of ribosomal RNA is an essential step in ribosome biogenesis. It strongly affects the total amount of ribosome production, and thus rapidly growing cells have an elevated level of ribosomal RNA transcription. Ribosomal RNA transcription is controlled by many mechanisms, including the efficiency of preinitiation complex formation for RNA polymerase I (Pol I) and epigenetic marks in ribosomal RNA genes. These are affected by cell cycle progression, signal transduction pathways, cell-damaging stresses, nutrients such as glucose, and the metabolites. Recent studies also suggest that the epigenetic marks, acetylation and methylation, may be not only controlled by nutrients but also function as reservoirs for biological resources in chromatin. Further studies would provide information about the mechanisms cells use to adjust production of cellular components to available resources and clues for developing novel anti-cancer treatments.

Keywords

  • ribosomal RNA (rRNA)
  • transcription
  • nutrients
  • glucose
  • epigenetic

1. Introduction

The ribosome is a unique machine for synthesizing protein in organisms. Protein synthesis is essential for all biological events, and the quantity of ribosomes substantially affects all biological activities. Rapidly growing cancer cells require synthesis of much protein and thus many ribosomes. In vertebrates, a ribosome consists of about 80 proteins and 4 structural ribosomal RNAs (rRNAs): 5S rRNA, 5.8S rRNA, 18S rRNA, and 28S rRNA [1, 2]. The construction processes are exceedingly complex and include rRNA transcription, rRNA processing, synthesis of ribosome proteins and regulatory proteins, assembly of rRNAs and ribosome proteins, and maturation of the ribosome ( Figure 1 ). The entire process consumes up to 80% of the cell’s materials [3, 4], and 80% of the energy to proliferate cells [4, 5]. The materials for ribosome production are supplied by nutrients that are taken up from the environment. Therefore, the production of ribosomes is restricted by environmental conditions, and cells should have control of the mechanisms of ribosome production in order to reconcile demands for cell activities with the available biological resources.

Figure 1.

Processes of ribosome construction. Ribosomal RNA transcription, processing, and association of ribosomal proteins occur in the nucleolus. Mature ribosome functions in the cytoplasm. The process is outlined in the yellow box on the right side of this figure. Ribosomes contain four structural ribosomal RNAs (rRNAs): 5S rRNA, 5.8S rRNA, 18S rRNA, and 28S rRNA. The first step of ribosome construction is transcription of ribosomal RNA (rRNA) from the ribosome RNA gene (rDNA) repeating units in the nucleolus. RNA polymerase I (Pol I) transcribes pre-rRNA, which is processed to three structured rRNAs (18S, 28S and 5.8S rRNA). 5S rRNA is synthesized by RNA polymerase III. In the mature ribosome, 18S rRNA is contained in the 40S ribosome (small subunit of ribosome), and 28S, 5.8S, and 5S rRNAs are contained in the 60S ribosome (large subunit of ribosome). Ribosomal protein assembly, rRNA processing, and maturation occur in the nucleolus, and ribosomes are exported to the cytoplasm and perform the translation activity.

Three of the four structured rRNAs (18S, 28S, and 5.8S rRNA) constituting ribosomes are produced by processing of a precursor transcript, pre-ribosomal RNA (pre-rRNA). The pre-rRNA is coded by rRNA genes (rDNA) and specifically transcribed by RNA polymerase I (Pol I) in the nucleolus. Because a single copy of rDNA is not sufficient to supply the number of rRNA molecules required, there are 100–300 copies of tandemly repeated rDNAs per haploid genome in mammals. Paradoxically, only half the copies of rDNA are in transcriptionally active forms and the rest are silent, which may provide a control step for rRNA transcription [6, 7, 8, 9]. The transcription of rRNA is an essential step in ribosome biogenesis and affects the total number of ribosomes produced. It was suggested that 75% of total RNAs constitute rRNAs in Hela cells [10], and the rRNA transcription represents about 35% of all transcripts in proliferating cells [6], showing that rRNA synthesis uses a lot of materials. Therefore, the control of rRNA transcription plays a role in maintaining homeostasis in biological resources. In this review, we describe the control of rRNA transcription by various factors such as the cell cycle regulators, signal transduction pathways, growth factors, tumor-related proteins, and cell-damaging stresses. Then, we will discuss the control mechanisms of rRNA transcription in response to nutrients.

2. Formation of preinitiation complex (PIC) on rDNA promoter

The first step of rRNA transcription is the formation of the preinitiation complex (PIC) on the rDNA promoter. The upstream binding factor (UBF), the promoter selective factor 1 (SL1), transcription initiation factor IA (TIF-IA), and RNA polymerase I (Pol I) synergistically assemble at the rDNA promoter to form PIC ( Figure 2 ). While the name SL1 is used for human proteins, that for mice is transcription initiation factor 1B (TIF-IB). UBF and SL1 bind to an upstream control element (UCE) and the core promoter region of rDNA, respectively. SL1 recruits Pol I through TIF-IA, and UBF stabilizes the binding of SL1 to Pol I at the rDNA promoter [6, 11].

Figure 2.

Pre-initiation complex for RNA polymerase I and rRNA processing. The basic composition of the pre-initiation complex (PIC) for RNA polymerase I is illustrated. PIC is assembled on the rDNA promoter by synergistic action of the upstream binding factor (UBF), which is bound at the upstream control element (UCE), selective factor 1 (SL1), which is bound to the core promoter through TATA-box binding protein (TBP), transcription initiation factor IA (TIF-IA), and RNA polymerase I (Pol I). SL1 contains TBP and Pol I-specific TBP-associated factors (TAFIs: TAFI110, TAFI68, TAFI48, TAFI41, and TAF12). SL1 on the core promoter recruits RNA polymerase I through TIF-IA, which associates with both components of Pol I and SL1. After the completion of PIC formation, Pol I is released from the promoter by regulation of TIF-IA and starts to transcribe pre-rRNA. This release is the initiation step of rRNA transcription. Pre-rRNA is processed to structured rRNA, 18S, 5.8S, and 28S rRNA to construct ribosomes.

SL1 is a protein complex consisting of TATA-box binding protein (TBP), Pol I-specific TBP-associated factors (TAFIs), including TAFI110, TAFI68, and TAFI48, which were originally identified with TBP as essential transcriptional factors by an in vitro transcription assay [12]. TBP is not only a Pol I-specific factor, but also used for transcription by RNA polymerase II and III. Later, TAFI41 and TAF12 were identified as members of the TAFIs. TAF12 was originally reported as a transcription factor for RNA polymerase II [13]. One activity of TBP is binding to the TATA box in the core promoter to recruit SL1 complex. TIF-IA binds to the RPA43 subunit of Pol I complex and TAFI110, TAFI68, and TAFI41 of the SL1 complex. These binding activities are essential for TIF-IA to recruit Pol I to the promoter bound by SL1 to facilitate PIC formation on the rDNA promoter. The formation of PIC is controlled by various factors ( Figure 3 ). When Pol I starts the rRNA transcription, the interactions forming PIC are disrupted. This disruption is the initiation step of transcription, one of the control mechanisms.

Figure 3.

Factors regulating PIC formation. The PIC for Pol I transcription is controlled by various factors, including cell cycle signals, signal transduction pathways, stress signals, oncoprotein/tumor suppressors, and others. The classes of regulatory factors are expressed with specific color boxes as indicated in the box on the right. UBF is activated by cdk family proteins, ribosomal protein S6 kinase (S6K), casein kinase II (CK2), and CBP, and is repressed by the Rb/HDAC complex. SL1 is activated by c-Myc, PCAF, and cdc14B, and repressed by p53, SIRT1,cdc2/cyclin B, CK2, PTEN, and GSK3β. TIF-IA is activated by ERK1/2, RSK, mTOR, and CK2 (activated by Akt), and repressed by JNK2 and AMPK. RNA polymerase I (Pol I) is activated by SIRT7 and CBP.

3. Control of rRNA transcription during cell cycle progression

The activities of all classes of RNA polymerases are controlled during the cell cycle progression [14, 15]. The cell cycle regulator cyclin/cdk complexes control the level of rRNA transcription ( Figure 3 ). In the M phase, SL1 is inactivated by cdk1/cyclin B (cdc2/cyclin B) through phosphorylation of TAFI110 to silence rRNA transcription [16, 17]. On exiting mitosis, the phosphorylation in TAFI110 is removed by cell division cycle 14B (Cdc14B) [18]. Additionally, mitotic repression of rRNA transcription correlates with the hypo-acetylation of TAFI68 caused by Sirtuin 1 (SIRT1). The hypo-acetylation makes SL1 instable on binding to the rDNA promoter [18]. It was also reported that the site of deacetylation of TAFI68 by SIRT1 is acetylated by p300/CBP-associated factor (PCAF), which is correlated with the activation of rRNA transcription [18].

After mitosis, rRNA transcription is re-activated by G1/S-specific cyclins (cdk4/cyclin D, cdk2/cyclin E, cdk2/cyclin A) through phosphorylation of UBF on the specific sites (cdk4/cyclin D (S484), cdk2/cyclin E (S388, S484), cdk2/cyclin A (S388): in mouse) [19, 20].

4. Signal transduction pathways control rRNA transcription

Protein synthesis is required for cell growth, and the signal transduction pathways that affect cell growth, including phosphoinositide 3-kinase (PI3K)-AKT-mammalian target of rapamycin (mTOR) signaling and ERK (MAPK) signaling, are involved in the regulation of rRNA transcription ( Figure 3 ).

The PI3K-AKT-mTOR signal pathway is stimulated by binding of insulin/Insulin-like growth factors (IGF) to their cognate receptors on the cell surface. AKT activates rRNA transcription through the phosphorylation of CK2. CK2 regulates rRNA transcription at multiple levels by affecting the formation of PIC, initiation, elongation, and reinitiation, through phosphorylation of several proteins including UBF, TAFI110 (SL1), and TIF-IA [21, 22, 23, 24, 25, 26]. mTOR activates rRNA synthesis by translocating TIF-IA into the nucleolus using kinase activity [27]. The ribosomal protein S6 kinase (S6K), which is a downstream kinase of mTOR, also activates rRNA synthesis through regulation of UBF-SL1 interaction by phosphorylation of UBF. The mTOR activity also enhances the expression of UBF [28]. SNF2 histone linker PHD RING helicase (SHPRH), which was identified as a RAD5 homolog and known as E3 ubiquitin-protein ligase, binds to rDNA promoters using its PHD domain and promotes recruitment of Pol I to rDNA ( Figure 4 ). This activation of rRNA transcription by SHPRH is inhibited in an mTOR-dependent manner [29]. K-demethylase 4A (KDM4A)/JMJD2A activates rRNA transcription on serum stimulation ( Figure 4 ). This activation is mediated through the PI3K/serum/glucocorticoid regulated kinase 1 (SGK1) signaling cascade independent of the AKT pathway. SGK1 is one of the downstream kinases of PI3K signaling. The serum-stimulated KDM4A decreases a repressive histone H3K9me3 mark modification in rDNA to activate rRNA transcription [30]. In mouse adipocytes, polymerase I transcription and release factor (PTRF)/Cavin-1 promotes rRNA transcription, which is induced by insulin and repressed by fasting ( Figure 4 ). The stimulation of rRNA transcription by PTRF is mediated by the formation of the transcription loop that links the transcriptional start sites and termination sites. The formation may enhance transcriptional reinitiation [31].

Figure 4.

Signal transduction pathways under growth factor controlled rRNA transcription. Growth factors including insulin, insulin-like growth factor (IGF-1), epidermal growth factor (EGF), and unidentified serum factors (Serum) control rRNA transcription through signal transduction pathways, such as PI3K/mTOR, PI3K/SGK and ERK1/2, which control PIC components (SL1, TIF-IA, and Pol I), transcription factors (PTRF/Carvin-1, c-Myc and SHPRH) or chromatin/nucleosome regulators (lncRNA, NuRD, and KDM2B).

The binding of epidermal growth factor (EGF) or its related ligands to their cognate receptors on the cell surface stimulates a signaling cascade including the GTPase Ras, the kinases Raf, MAP kinase-ERK kinase (MEK), and extracellular signal-regulated kinase (ERK). ERK activates rRNA transcription through phosphorylation of UBF in the promoter [32] and gene body regions [33] ( Figure 3 ).This phosphorylation decreases the binding capacity of UBF to rDNA. In this case, it was reported that the dissociation of UBF from rDNA enhances Pol I release from the promoter, leading to activation of rRNA transcription. Additionally, ERK/90 kDa ribosomal S6 kinase (RSK) phosphorylates TIF-IA to activate rRNA transcription [34] ( Figure 3 ). It is still unclear how the phosphorylation by RSK induces rRNA transcription.

Elevation of the concentration of calcium ions (Ca2+) in the cytoplasm stimulates the signaling pathway of calcium/calmodulin-dependent protein kinase II (CaMKII). The stimulated CaMKII activates S6K, which phosphorylates UBF to activate rRNA transcription in colorectal cancer (CRC) ( Figure 3 ). In CRC, the function of adenomatous polyposis coli (APC) gene is frequently lost and the level of Ca2+ is increased in the cells [35].

5. Control of rRNA transcription by unidentified serum factors

Serum, used to supplement the cell culturing medium, contains many factors that control rRNA transcription. Although all factors and signal cascades are not completely identified, they perform critical functions in the regulation of rRNA transcription ( Figure 4 ).

Depletion of serum from a culture medium represses rRNA transcription. c-Myc plays a critical role for cell growth and proliferation in many types of cells, and is deregulated and overexpressed in tumor cells. c-Myc associates with the promoter and transcribed regions of rDNA and activates rRNA transcription in response to serum stimulation [36].

K-demethylase 2B (KDM2B)/JHDM1B is bound to rDNA to repress rRNA transcription. The repression is associated with the demethylation of trimethylated lysine 4 on histone H3 (H3K4me3) by KDM2B. Serum starvation increases the recruitment of KDM2B on rDNA, and resupply of serum decreases it. These data suggest that the activity of KDM2B in controlling rRNA transcription is regulated by serum factors [37].

The specific long non-coding RNAs (lncRNAs) are induced during periods of quiescence, such as serum starvation, and increase the level of histone H4K20me3 on the rDNA promoter in a suppressor of variegation 4-20 homolog (Suv4-20 h)-dependent manner. The elevated level of H4K20me3 leads to chromatin compaction. The lncRNAs are antisense transcripts against rDNA, are termed the promoter and pre-rRNA antisense (PAPAS), and associated with rDNA [38].

A chromatin remodeling complex, nucleosome remodeling deacetylase (NuRD), establishes the poised state of rDNA through regulation of histone modifications and nucleosome positions. The level of the state of rDNA is increased in the growth-arrested conditions induced by serum starvation and differentiation [39].

6. Oncoprotein and tumor suppressors in rDNA transcription

Tumor cells show abnormal growth that is thought to be associated with the elevation of ribosome biogenesis, and regulation of rRNA transcription by oncogenes and tumor-suppressor genes was reported.

The oncoprotein c-Myc is the product of oncogene c-myc, and its expression is stimulated by serum and associated with rRNA transcription [36] ( Figure 4 and Section 5). It was reported that c-Myc binds to the sites with the consensus sequences on rDNA and stimulates rRNA transcription [40]. c-Myc is also reported to control the PIC factors such as UBF and ribosomal proteins [41, 42, 43]. Therefore, c-Myc activates ribosome biogenesis at multiple steps [3, 44, 45] .

The rb gene is a tumor-suppressor gene. Rb protein binds to UBF, which may be related to restriction of cell proliferation by Rb [46]. The binding of Rb to UBF inhibits the binding of UBF to rDNA [47] or inhibits the binding of UBF to SL1 [48], both of which result in the repression of rRNA transcription ( Figure 3 ). Another report suggested that phosphorylated Rb (pRb) creates a complex with histone deacetylase (HDAC) and decreases the acetylation of UBF to repress rRNA transcription [49]. In this study, it was also reported that the acetylation in UBF is modified by CREB-binding protein (CBP) ( Figure 3 ).

A tumor-suppressor gene, p53, is frequently mutated in tumors, and p53 protein represses rRNA transcription through prevention of the interaction between SL1 and UBF [50] ( Figure 3 ).The phosphatase and tensin homolog deleted from chromosome 10 (PTEN) is known as a tumor suppressor. PTEN represses rRNA transcription by disrupting the SL1 complex in its lipid phosphatase activity-dependent manner [51] ( Figure 3 ). It was also reported that PTEN is phosphorylated by glycogen synthase kinase (GSK) 3β [52]. GSK3β and PTEN are selectively enriched in the nucleoli of RAS-transformed cells and associate with the promoter and coding region of the rDNA [53]. An activated GSK3β mutant abolishes rRNA transcription and associates with TAFI110 in the SL1 complex [53]. These results suggest a repressive function for GSK3β on rRNA transcription that supports its role as a tumor suppressor.

7. Controls of rRNA transcription by cell-damaging stresses

A variety of stresses such as UV, ionizing radiation, heat shock, and osmotic shock attack cellular vital components like DNA, proteins, and lipid membranes. These stresses also affect rRNA transcription.

c-Jun N-terminal kinase (JNK) phosphorylates c-Jun at the NH2-terminal Ser63 and 73 residues in response to UV irradiation and other stress stimuli [54]. JNK2 inactivates rRNA transcription through phosphorylation of TIF-IA to inhibit its function of bridging between Pol I and SL1 [55] ( Figure 3 ).

The DNA damage caused by ionizing radiation also induces the repression of rRNA transcription through other pathways, which involves Nijmegen breakage syndrome protein 1 (NBS1)-treacle, Ataxia Telangiectasia Mutated (ATM), and breast cancer susceptibility gene I (BRCA1). In the presence of double strand breaks induced by ionizing radiation, NBS1 translocates and accumulates in nucleoli in a treacle-dependent manner to silence rRNA transcription [56]. The treacle gene was found to be mutated in Treacher Collins syndrome, which is characterized by deformation of bones and other tissues in the face. ATM-dependent signaling was shown to shut-down rRNA transcription in response to chromosome breaks [57]. BRCA1, known as a tumor suppressor, was reported to interact with UBF, SL1, and Pol I. In response to DNA damage, BRCA1 bound to rDNA is dissociated, and induces instability of Pol I on rDNA to repress rRNA transcription [58].

Heat shock at 42°C represses rRNA transcription through the inactivation of TIF-IA by inhibition of CK2-dependent phosphorylation of TIF-IA and the lncRNAs PAPAS-dependent nucleosome regulation by NuRD complex [59]. Hypotonic stress represses rRNA transcription through upregulation of PAPAS to trigger nucleosome repositioning by NuRD [60]. In these conditions, Suv420h2 was neddylated and the levels of Suv420h2 and H4K20me3 marks were increased. However, the relationship between PAPAS and Suv420h2 was not clear. Cytoskeletal stress, which is related to cell shape, represses rRNA transcription through Rho-associated protein kinase (ROCK). ROCK is one of the kinases of myosin and induces recruitment of HDAC on rDNA, resulting in deacetylation of histone acetylated lysine 9 and 14 on histone H3 (H3K9/14) [61].

8. Control of rRNA transcription by nutrients

Cells obtain biological resources for cellular activities from their environment. The sensing of environmental nutrients is important for efficient usage of nutrients and maintaining cells. In murine intestinal epithelium, apical transcripts are more efficiently translated, because ribosomes were more abundant on the apical sides. Refeeding of fasted mice induces a basal to apical shift of mRNAs encoding ribosomal proteins, which is associated with an increase in their translation and increased protein production. These mechanisms allow efficient nutrient absorption in response to the rich conditions, although the molecular mechanisms are not clear [62]. It was shown that mTOR senses the levels of amino acids, especially leucine, in cells, and controls the translation activity through regulation of the eukaryotic translation initiation factor 4E binding protein (4E-BP)-eukaryotic translation initiation factor 4E (elF4E) axis and p70 S6K-S6 axis [4, 63, 64]. Recently, increasing evidence shows the presence of specific mechanisms to control rRNA transcription in response to nutrients ( Figure 5 ).

Figure 5.

Glucose and amino acids control rRNA transcription. Glucose, amino acids, and GTP control rRNA transcription through several pathways, such as ERK, mTOR/S6K, and AMPK, which control PIC components (UBF, SL1, and TIF-IA), transcription factors (c-Myc), or chromatin regulators (KDM2A, PIH1, and SIRT1/NML). High glucose activates ERK and mTOR/S6K pathways to control UBF. High glucose also activates PIH1 to control chromatin. Glucose depletion or low energy conditions activate SIRT1/NML and the AMPK pathway. Activated AMPK controls the activities of TIF-IA and KDM2A. Activated KDM2A controls rDNA chromatin to inhibit rRNA transcription. Amino acid depletion represses the mTOR pathway, resulting in the repression of TIF-IA, and decreases the expression of c-Myc. Guanosine triphosphate (GTP) is bound to TIF-IA, and the binding is required to control rRNA transcription.

8.1. Amino acids

The starvation of amino acids affects the frequency of initiation of nucleolar RNA polymerase, which was later established to be an rRNA transcription by Pol I [65]. The starvation of amino acids decreases the interaction of TIF-IA with SL1 and Pol I [66]. As described above in Section 4, mTOR controls rRNA transcription, and is important for regulation of rRNA transcription in response to amino acid levels. Amino acid starvation inhibits the activity of mTOR and its downstream kinase S6K. mTOR and S6K control TIF-IA and UBF, respectively, to regulate rRNA transcription [27, 67].

c-MYC is also involved in regulation of rDNA transcription in response to starvation of amino acids. Although translation of c-Myc is reported to be controlled by mTOR signaling [68, 69], the stabilization of c-Myc in response to amino acid starvation is controlled by an mTOR-independent pathway [70].

8.2. Guanosine triphosphate (GTP)

It was reported that the sizes of ATP and GTP intracellular pools affect the level of nucleolar RNA synthesis (rRNA transcription) [71]. Recently, the consensus sequences for GTP binding were identified in TIF-IA [72], and the binding of TIF-IA to GTP is required for the interaction of TIF-IA with ErbB3-binding protein (Ebp1). Ebp1 controls ribosomal biogenesis when located in the nucleolus [73]. Therefore, the level of GTP appears to be sensed by TIF-IA to affect rRNA transcription ( Figure 5 ).

8.3. Glucose

The major energy source for cells is glucose. Glucose is used to synthesize ATP. ATP is essential for most biological activities, including ribosome biogenesis. Several studies demonstrated that the levels of glucose and ATP production affect rRNA transcription.

Ribosomal biogenesis including rRNA transcription was reported to be induced by high glucose treatment or diabetes. A high level of glucose activates UBF through ERK1/2 and mTOR in kidney glomerular epithelial cells of mice [74].

The PIH1 domain-containing protein 1 (PIH1)/Nop17 is reported to enhance rRNA transcription through the recruitment of SNF5-Brg1 complex on the rRNA promoter [75] ( Figure 5 ). The complex increases acetylation of several histones, except histone H4K16Ac, on rDNA in high glucose conditions. Until now, the acetylation marks of histone in rDNA, excluding the acetylation at K16 in histone H4 (H4K16Ac), are linked to activation of transcription. The acetylated histones function as active marks in transcription in many cases because the acetylation of histone weakens the interaction of histone octamers with DNA, and the acetylated histones are recognized by several transcription-activating factors. On the other hand, the H4K16Ac mark is reported to be recognized by nucleolar remodeling complex (NoRC) in rDNA, which induces chromatin-silencing status [76]. Glucose starvation dissociates PIH1 and the SNF5-Brg1 complex from rDNA and increases histone H4K16Ac marks, which repress rRNA transcription [75]. Another report suggested that PIH1 interacts with mTORC1 to stabilize it, resulting in enhancement of rRNA transcription [77].

8.4. AMPK is activated by glucose starvation

Glucose starvation decreases ATP production and activates AMPK ( Figure 5 ). The AMP-activated kinase (AMPK) is known as an energy sensor, which recognizes the ratios of AMP, ADP, and ATP and regulates many phenomena in cells to maintain energy homeostasis.

Additionally, a recent study showed the existence of an AMP/ADP-independent mechanism that triggers AMPK activation ( Figure 6 ). Glycolysis is a determined sequence of 10 enzyme-catalyzed reactions. In the fourth step, the hexose ring of fructose 1, 6-bisphosphate (FBP) is split by aldolase into two triose sugars: dihydroxyacetone phosphate (a ketose) and glyceraldehyde 3-phosphate (an aldose). When extracellular glucose is decreased, intracellular FBP is decreased, and aldolase unoccupied by FBP promotes the formation of a lysosomal complex containing v-ATPase axin, liver kinase B1 (LKB1), and AMPK, which regulates AMPK activity [78]. These results suggest that the decreased level of the metabolite in glycolysis controls AMPK before the reduction of ATP production just after changing environmental conditions, emphasizing that AMPK is a highly sensitive monitor of energy conditions.

Figure 6.

Control of methylation by energy status and methyl marks as a reservoir for biological resources. Chromatin components are methylated and demethylated by specific enzymes influenced by metabolites in energy production. Formaldehyde is produced as a demethylation byproduct, directly generates one carbon unit, fuels the folate cycle through alcohol dehydrogenase 5 (ADH5) activity, and can be used as a source for production of SAM, which is used for methylation and nucleotides. AMPK, AMP-activated protein kinase; DHF, dihydrofolate; FBP, fructose 1, 6-bisphosphate; 3PG, 3-phosphoglycerate; SAM, S-adenosylmethionine; THF, tetrahydrofolate.

AMPK induces phosphorylation of TIF-IA ( Figure 5 ). The phosphorylation of TIF-IA by AMPK reduces interaction of TIF-IA with SL1, decreases the TIF-IA amount on the rDNA promoter, and interrupts PIC assembly, which results in the reduction of rRNA transcription [79].

KDM2A, identified as mono- and di-methylated lysine 36 on histone H3 (H3K36me1/2) demethylase [80], is accumulated in the nucleolus and binds to rDNA [81]. The repression of rRNA transcription by KDM2A is induced in response to serum and glucose starvation ( Figure 5 ). The repression requires the demethylase activity of KDM2A on the rDNA promoter [82]. The KDM2A-dependent regulation affects the levels of protein synthesis [81]. The demethylase activity of KDM2A proceeds with a co-reaction in which α-ketoglutarate (α-KG) is converted to succinate, both of which are organic acids constituting the TCA cycle. Interestingly, the enzyme activity of KDM2A is controlled by cell-permeable succinate (dimethyl succinate: DMS), suggesting that metabolites in the TCA cycle affect KDM2A activity. Recently, it was found that glucose starvation in the presence of serum induces the repression of rRNA transcription by KDM2A, in which activated AMPK induces KDM2A activity [83] ( Figure 5 ). Interestingly, treatment with a low concentration of the glycolysis inhibitor 2-deoxy-D-glucose (2DG) induces KDM2A-dependent repression of rRNA transcription associated with histone demethylation on the rDNA promoter, although it does not dissociate TIF-IA from the rDNA promoter. Treatment with a high concentration of 2DG induces both the dissociation of TIF-IA from the rDNA promoter and KDM2A-dependent demethylation of the rDNA promoter. These results suggest that the repression of rRNA transcription in response to glucose starvation is performed by two different mechanisms: epigenetic regulation by KDM2A and TIF-IA regulation, depending on the glucose starvation level.

AMPK phosphorylates dozens of proteins, but until now KDM2A has not been detected as a substrate of AMPK kinase activity. AMPK also controls the activity of mTOR [84], and mTOR is a candidate kinase for control of the states of histone methylation in the rDNA promoter, but currently there is no evidence connecting mTOR and KDM2A. Further studies are required to determine how the KDM2A activity in the rDNA promoter is induced by AMPK. H3K36me2 on the rDNA promoter which is demethylated by KDM2A on starvation is quickly restored by refeeding glucose and serum [82]. The data suggest that the control of H3K36me2 levels on rDNA promoters is reversible by changes in nutrient status, although which enzyme induces methylation of H3K36me2 on the rDNA promoter in response to refeeding of glucose and serum remains unknown. The control mechanism of rRNA transcription through epigenetic regulation by KDM2A may be a fine tuning device that quickly reflects nutrient states around cells.

8.5. Sirtuins

Sirtuins target a wide range of cellular proteins in the nucleus, cytoplasm, and mitochondria for post-translational modification by acetylation (SirT1, 2, 3, and 5) or ADP-ribosylation (SirT4 and 6). The deacetylase activity of sirtuins is controlled by the cellular NAD+/NADH ratio, where NAD+ works as an activator, while nicotinamide and NADH act as inhibitors ( Figure 7 ). The acetylation regulates a wide variety of cellular functions. Sirtuins participate in various cellular processes, deacetylating both chromatin and non-histone proteins, and their roles in aging have been extensively studied. Sirtuins may also play a critical role in tumor initiation and progression as well as drug resistance. Reduced compounds such as glucose and fatty acids are oxidized, thereby releasing energy. This energy is transferred to NAD+ by reduction to NADH, as part of glycolysis, the citric acid cycle, and β-oxidation. The mitochondrial NADH is then oxidized in turn by the electron transport chain, which generates ATP through oxidative phosphorylation.

Figure 7.

Control of acetylation by energy status and acetyl marks as a reservoir for biological resources. Chromatin components are acetylated and deacetylated by specific enzymes whose activities are influenced by metabolites in energy production. During deacetylation, the acetyl group from the substrate is accepted by the ribose to produce O-acetyl-ADP-ribose (OAADPr). A cytoplasmic esterase, which was suggested to be ADP-ribosyl hydrolase 3 (ARH3), hydrolyzes OAADPr to acetate and ADPr. Acetate that is generated from the deacetylation may be changed to acetyl-CoA, and used as resource for acetyl marks and lipogenesis. ACECSI, acetyl-CoA synthase 1; ACL, ATP-citrate lyase; OAADPr, O-acetyl ADP-ribose.

SIRT1 was reported to be required for the recruitment of nucleomethylin (NML) on rDNA. In response to glucose starvation, rRNA transcription is repressed through NML-induced chromatin regulation [85]. Although it is not clear that SIRT1 shows deacetylase activity on starvation, SIRT1 induces the deacetylation of p53, and this deacetylation activity is required for the repression of rRNA transcription. Further, the NAD+ synthesis enzyme nicotinamide mononucleotide adenylyltransferase (NMNAT1) modulates the repression of rRNA transcription [86]. As described above, SIRT1 is required for mitotic repression of rRNA transcription through deacetylation of TAFI68 ( Figure 3 and section 3). TAFI68 is acetylated by PCAF to restart transcription in the mitotic exit phase [18].

On the other hand, SIRT7, another SIRT family member, was reported to activate rRNA transcription depending on the deacetylation activity, through regulation of PAF53, which is an important component of Pol I complex [87, 88, 89, 90, 91].

8.6. Epigenetic marks may play a role in conserving biological resources

Acetyl-CoA is used as an acetyl group donor on acetylation of histone and other proteins ( Figure 7 ). Acetyl-CoA is produced from pyruvate, acetate, or fatty acid oxidation in multiple metabolisms. The amount of acetyl-CoA affects the activity of histone acetyltransferases (HATs). S-adenosylmethionine (SAM) is known to be used as a methyl donor in DNA/histone (protein) methylation ( Figure 6 ). SAM is produced through the condensation of methionine and ATP by methionine adenosyltransferase (MAT). The production of SAM is mediated through the folate and methionine cycles. The amount of SAM is thought to affect the activity of methylase. The production of the two epigenetic marks is clearly affected by energy production processes, suggesting that intracellular energy conditions affect the modifications of epigenetic marks [92, 93, 94].

Further, enzymes detaching the marks are also affected by metabolites in energy production. NAD+ activates sirtuin deacetylase, while nicotinamide and NADH inhibit the activity ( Figure 7 ). The demethylase activity of KDM2A is controlled by the amounts of intracellular ATP through AMPK ( Figure 6 ), and probably more directly by succinate. In another report, chromatin-associated fumarase generating fumarate inhibits the demethylation activity of KDM2A on the promoter region of the RNA polymerase II gene [95]. Additionally, 2-hydroxy-glutarate (2-HG), which was produced from α-KG by a mutant type isocitrate dehydrogenase (IDH) also modulates several jmjC-type enzymes including the lysine-specific demethylases (KDMs) such as KDM2A and Tet methyl-cytosine dioxygenases (TETs) [96, 97, 98]. Therefore, metabolites reflecting intracellular energy conditions can control the enzymes for detaching the epigenetic marks as well as adding them.

Interestingly, during deacetylation, the glycosidic bond of the nicotinamide ribose is cleaved to yield nicotinamide, and the ribose accepts the acetyl group from the substrate to produce O-acetyl-ADP-ribose (OAADPr). In vitro, a cytoplasmic esterase from humans and yeast, which was suggested to be ADP-ribosyl hydrolase 3, hydrolyzes OAADPr to acetate and ADPr [99]. Therefore, acetyl marks of proteins that are deacetylated by sirtuin may also be used as a source of acetate for acetyl marks and lipogenesis ( Figure 7 ). KDMs catalyze histone lysine demethylation through an oxidative reaction. The catalytic reaction begins with the coordination of molecular oxygen (O2) by Fe(II) and the conversion of α-KG to succinate and CO2 with the concomitant hydroxylation of the methyl group of the peptide substrate. The resulting carbinolamine is unstable and degrades spontaneously to an unmethylated peptide and the cytotoxic molecule formaldehyde ( Figure 6 ). Recently, it was shown that formaldehyde reacts spontaneously with glutathione (GSH) to yield S-hydroxymethylglutathione (HMGSH), and subsequently HMGSH is oxidized by alcohol dehydrogenase 5 with NAD(P)+ to create S-formylglutathione. This biochemical route provides a cell with formaldehyde detoxification as well as utilizable one-carbon units, which contribute to nucleotide synthesis. Therefore, cells reserve materials for one-carbon metabolism as methyl marks [100], which are released by KDM2A on starvation. These two examples of acetylation and methylation suggest that modifications of the epigenetic marks are not only controlled by intracellular energy conditions, but also function as reservoirs in chromatin for biological resources.

9. Applications for therapy

Enlarged nucleoli have been recognized as a hallmark of cancer cells [101, 102]. Elevated levels of rRNA transcription and protein synthesis are often observed in cancer cells. These observations suggest the possibility that the control of rRNA transcription could regulate the proliferation of cancer cells. Actually, the anti-cancer effects of some compounds are associated with down-regulation of rRNA transcription. Cisplatin [103], mitomycin C [104], and 5-fluorouracil [105], well-known anti-cancer drugs, are reported to inhibit rRNA transcription [11]. However, it is not clear whether the reduction of rRNA transcription in cancer cells is causal or only as a consequence of inhibition of cell growth.

On the other hand, there are drugs that appear to reduce rRNA transcription and then repress cancer growth. Actinomycin D (Dactinomycin) specifically represses rRNA synthesis at low concentrations through inactivation of transcriptional elongation by Pol I by interaction with GC-rich regions of rDNA, and thus inhibits growth of cancer cells. Actinomycin D is used as a chemotherapy medication to treat a number of types of cancer, including gestational trophoblastic neoplasia [106], Wilms tumor [107], rhabdomyosarcoma [108], Ewing’s sarcoma [109], and malignant hydatidiform mole [110].

CX-5461 was identified by screening for selective inhibitors of Pol I but not Pol II transcription. CX-5461 specifically inhibits ribosomal RNA transcription by impairment of SL1 binding to the rDNA promoter [111] and thus exhibits anti-cancer activity [83, 111]. The inhibition of rRNA synthesis by CX-5461 leads to senescence and autophagy in a p53-independent manner in a tumor cell line [111], to activation of p53-dependent apoptotic signaling in Myc-overexpressing B-lymphoma cells (Eμ-Myc lymphoma cells) [112], and to activation of the ATM/Ataxia Telangiectasia and Rad3-related protein (ATR) pathway in acute lymphoblastic leukemia to induce G2 arrest and apoptosis [113]. The potential therapeutic effect of CX-5461 was demonstrated in xenograft models using human pancreatic carcinoma (MIA PaCa-2), melanoma (A375) [111], biphenotypic B myelomonocytic leukemia (MV 4;11) [112] and breast cancer susceptibility gene II (BRCA2) deficient colon cancer (HCT116) [114], and in mice models transplanted with p53 wild-type Eμ-Myc lymphoma [112]. The treatment of CX-5461 in these experiments hardly affected on the health and body weights of mice [111, 112].

BMH-21, which was identified by cell-based screening, intercalates into GC-rich sequences, which exist at a high frequency in rDNA, and represses Pol I transcription [115]. Treatment with BMH-21 induces proteasome-dependent degradation of the largest catalytic subunit of Pol I, RPA194, resulting in a decrease of the Pol I level on rDNA. These effects were correlated to the anti-cancer activity of BHM-21. The anti-tumor activity of BMH-21 was demonstrated using human melanoma (A375) and colorectal carcinoma (HCT116) xenograft models with little effect on body weight [115]. These studies suggest that the chemicals that repress the rRNA transcription show anti-cancer activities.

Epigenetic controls of rDNA chromatin are also candidates for cancer therapy. For example, specific activation of KDM2A could reduce cancer cell proliferation. Because KDM2A activity is regulated by ATP levels through AMPK and also metabolites in energy production, control of these compound levels may regulate KDM2A activity and cell proliferation. As seen here, there are many elaborate mechanisms for control of rRNA transcription, some of which involve intracellular metabolites, which are produced from environmental nutriments. Further studies of the relationship between rRNA transcription and nutrients will provide information about the mechanisms by which cells reconcile demand and usage of biological resources, and clues for novel methods to treat cancers.

10. Conclusion

The construction of ribosomes consumes the majority of the cell’s materials and energy. Because the materials for ribosome production are supplied by nutrients, the production of ribosomes is largely restricted by environmental nutrients and cells need mechanisms to control ribosome production in order to reconcile demands for cell activities with available resources. Transcription of rRNA is an essential step in ribosome biogenesis, and strongly affects the total amount of ribosome production. Ribosomal RNA transcription is controlled by many mechanisms, including the efficiency of PIC formation for Pol I and epigenetic marks in rDNA. These are affected by nutrients. Recent studies suggest that the epigenetic marks, such as acetylation and methylation, may be not only controlled by nutrients but also function as reservoirs for biological resources in chromatin. Elevated levels of rRNA transcription and protein synthesis are often observed in cancer cells, and the control of rRNA transcription can regulate their proliferation. Indeed some chemicals that repress the rRNA transcription show anti-cancer activities. Further studies of the relationship between rRNA transcription and nutrients will provide clues for novel methods to treat cancers.

Acknowledgments

This work was supported by JSPS KAKENHI Grant numbers 17K07798 and 16K07358.

Abbreviations

AMPKAMP-activated kinase
ATMAtaxia telangiectasia mutated
CdkCyclin dependent kinase
CK2Casein kinase 2
ERKExtracellular signal-regulated kinase
FBPFructose 1, 6-bisphosphate
GSK3βGlycogen synthase kinase 3β
H3K36me2Dimethylated lysine 36 on histone H3
H4K20me3Trimethylated lysine 20 on histone H4
HDACHistone deacetylase
KDM2ALysine(K)-specific demethylase 2A
KDMsLysine(K)-specific demethylases
lncRNAsLong non-coding RNAs
mTORMammalian target of rapamycin
NuRDNucleosome remodeling deacetylase
PAPASPromoter and pre-rRNA antisense
PCAFp300/CBP-Associated factor
PI3KPhosphoinositide 3-kinase
PICPreinitiation complex
PIH1PIH1 domain-containing protein 1
Pol IRNA polymerase I
pre-rRNAPre-ribosomal RNA
PTENPhosphatase and tensin homolog deleted from chromosome 10
rDNARibosome RNA gene
rRNARibosomal RNA
RSKERK/90 kDa ribosomal S6 kinase
S6KRibosomal protein S6 kinase
SAMS-adenosylmethionine
SIRT1Sirtuin 1
SL1Promoter selective factor 1
Suv420h2Suppressor of variegation 4-20 homolog
TAFIsTBP-associated factors for RNA polymerase I
TBPTATA-box binding protein
TIF-IATranscription initiation factor IA
UBFUpstream binding factor

© 2018 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Yuji Tanaka and Makoto Tsuneoka (February 28th 2018). Control of Ribosomal RNA Transcription by Nutrients, Gene Expression and Regulation in Mammalian Cells - Transcription Toward the Establishment of Novel Therapeutics, Fumiaki Uchiumi, IntechOpen, DOI: 10.5772/intechopen.71866. Available from:

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