Open access peer-reviewed Edited volume

Gel Electrophoresis

Principles and Basics

Edited by Sameh Magdeldin

Niigata University, Japan

Most will agree that gel electrophoresis is one of the basic pillars of molecular biology. This coined terminology covers a myriad of gel-based separation approaches that rely mainly on fractionating biomolecules under electrophoretic current based mainly on the molecular weight. In this book, the authors try to present simplified fundamentals of gel-based separation together with exemplarily applications of this versatile technique. We try to keep the contents of the book crisp and comprehensive, and hope that it will receive overwhelming interest and deliver benefits and valuable information to the readers.

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Gel ElectrophoresisPrinciples and BasicsEdited by Sameh Magdeldin

Published: April 4th 2012

DOI: 10.5772/2205

ISBN: 978-953-51-0458-2

chaptersDownloads

Open access peer-reviewed

1. Introduction to Agarose and Polyacrylamide Gel Electrophoresis Matrices with Respect to Their Detection Sensitivities

By Patricia Barril and Silvia Nates

30582

Open access peer-reviewed

2. Gel-Electrophoresis and Its Applications

By Pulimamidi Rabindra Reddy and Nomula Raju

15622

Open access peer-reviewed

3. Principles of Nucleic Acid Separation by Agarose Gel Electrophoresis

By Muhittin Yılmaz, Cem Ozic and İlhami Gok

26187

Open access peer-reviewed

4. Discriminatory Power of Agarose Gel Electrophoresis in DNA Fragments Analysis

By Seow Ven Lee and Abdul Rani Bahaman

28435

Open access peer-reviewed

5. Gel Electrophoresis of Proteins

By Laura García-Descalzo, Eva García-López, Alberto Alcázar, Fernando Baquero and Cristina Cid

9535

Open access peer-reviewed

6. Gel Electrophoresis of Protein - From Basic Science to Practical Approach

By Gholamreza Kavoosi and Susan K. Ardestani

5282

Open access peer-reviewed

7. Two-Dimensional Polyacrylamide Gel Electrophoresis - A Practical Perspective

By Sameh Magdeldin, Ying Zhang, Bo Xu, Yutaka Yoshida and Tadashi Yamamoto

9611

Open access peer-reviewed

8. High-Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis: A Tool for Identification of Polymorphic and Modified Linker Histone Components

By Andrzej Kowalski and Jan Pałyga

2565

Open access peer-reviewed

9. Two-Dimensional Gel Electrophoresis (2-DE)

By Bruno Baudin

3201

Open access peer-reviewed

10. High Speed Isoelectric Focusing of Proteins Enabling Rapid Two-Dimensional Gel Electrophoresis

By Gary B. Smejkal and Darren J. Bauer Part

2777

Open access peer-reviewed

11. Denaturing Gradient Gel Electrophoresis (DGGE) in Microbial Ecology - Insights from Freshwaters

By Sofia Duarte, Fernanda Cassio and Claudia Pascoal

10355

Open access peer-reviewed

12. Statistical Analysis of Gel Electrophoresis Data

By Kimberly F. Sellers and Jeffrey C. Miecznikowski

3922

Open access peer-reviewed

13. Quantitative Analysis of Electrophoresis Data - Application to Sequence-Specific Ultrasonic Cleavage of DNA

By Sergei Grokhovsky, Irina Il’icheva, Dmitry Nechipurenko, Michail Golovkin, Georgy Taranov, Larisa Panchenko, Robert Polozov and Yury Nechipurenko

1878

Open access peer-reviewed

14. The Use of Pulsed Field Gel Electrophoresis in Listeria monocytogenes Sub-Typing - Harmonization at the European Union Level

By Benjamin Felix, Trinh Tam Dao, Bertrand Lombard, Adrien Assere Anne Brisabois and Sophie Roussel

3253

Open access peer-reviewed

15. Electrophoretic Techniques in Microbial Ecology

By Elena Gonzalez-Toril, David Lara-Astiaso, Ricardo Amils and Angeles Aguilera

1851

Open access peer-reviewed

16. Application of Multiplex PCR, Pulsed-Field Gel Electrophoresis (PFGE), and BOX-PCR for Molecular Analysis of Enterococci

By Charlene R. Jackson, Lori M. Spicer, John B. Barrett and Lari M. Hiott

2799

Open access peer-reviewed

17. The Use of Pulsed Field Gel Electrophoresis in Listeria monocytogenes Sub-Typing - Comparison with MLVA Method Coupled with Gel Electrophoresis

By Sophie Roussel, Marie-Leone Vignaud, Jonass T Larsson, Benjamin Felix, Aurore Rossignol, Eva Moller Nielsen and Anne Brisabois

1467

Open access peer-reviewed

18. Restriction Fragment Length Polymorphism Analysis of PCR-Amplified Fragments (PCR-RFLP) and Gel Electrophoresis - Valuable Tool for Genotyping and Genetic Fingerprinting

By Henrik Berg Rasmussen

30238

Open access peer-reviewed

19. Application of Two-Dimensional Gel Electrophoresis to Microbial Systems

By Fatemeh Tabandeh, Parvin Shariati and Mahvash Khodabandeh

8944

Edited volume and chapters are indexed in

  • Worldcat
  • OpenAIRE
  • Google Scholar
  • AZ ebsco
  • Base
  • CNKI
  • BKCI
  • BIOSIS Previews

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198504total chapter downloads

62Web of Science citations

17Crossref citations

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