Rhizome length growth in rhizoboxes (rhizome pieces in cm); rhizoboxes 1 and 2 are positioned horizontally, and rhizoboxes 3 and 4 are positioned vertically (HBLFA Raumberg-Gumpenstein).
\r\n\tThe biological activities of the bioactive compounds are based on the lead or the privileged scaffold present in the structure. The different scaffolds present in natural bioactive compounds are indole, purine, chromone, coumarin, benzothiphene, lactone, etc. These privileged scaffolds modify into multiple molecules for having different bioactivity. Some of the bioactive compounds in large quantity have an adverse effect on health. Recently, bioactive compounds are widely used in green chemistry, nanotechnology, and metal chelation.
\r\n\tThe book provides a reference for a wide range including chemistry, analytical techniques, medicinal chemistry, pharmacology, nanotechnology, etc.
The pioneer works in this line of intervention and investigation arose in Cuba, in the decade of 1970. The late Dr. Eduardo B. Ordaz, the former director of the Havana Psychiatric Hospital, the mythical Prima Ballerina Assoluta Ms. Alicia Alonso, director of the Cuban Company “The National Ballet of Cuba” and the prestigious Cuban Psychologist, Dra. Georgina Fariñas, all together created Cuban psychotherapeutic method known as “Psicoballet” [1].
The Psicoballet is a tool, which combines science and art. This method uses art and its different expressions (dance, ballet, theater, and pantomime) to improve the quality of life of people with disabilities and psychiatric problems and, in some cases, to rehabilitate these patients and help them become incorporated into society. In 1984, after analyzing over 25,000 cases successfully treated using this method, the recognition was granted by the UNESCO with the establishment of this organization as the UNESCO Psicoballet Company of Cuba and appointed Georgina Fariñas as its director [2].
The Psicoballet arises as method of infantile psychotherapy to treat children with disorders of conduct that they were not evolving with play therapy and occupational activities. As method psychotherapeutic and psycho‐corrective, the Psicoballet was applied to pre‐school and school that were presenting disorders of conduct, as anxiety, hyperactivity, aggressiveness, isolation, and so on. In addition, the parents of these children received education in the school for parents.
From the year 1977, the Department of Public Health does a review of the method of the Psicoballet and verifies its therapeutic character; for what in the same year named officially and assigned to the Psychiatric Hospital of the Havana, already as department. Once the method was made official, they realized its normalization and introduced a code of practice for what, having perfected the methodology, its use is extending and in the treatment are included children and teenagers with mental delays, blind and visual problems, deaf weak and deaf/hard of hearing; adult and the elderly with moderate, severely, and deeply mental problems [3].
In February 1984, the National Commission of Cuba of the UNESCO constituted the group UNESCO of Psicoballet, due to the scientific welfare contribution and the achievements obtained in the treatment of the children, teenager, and adults with psychic, mental, motor, and sensory neuropathies. Today, its efficiency has led it to apply to a wide group of mental and neurotic severe patients and old people.
In 1989, in the Foundation Dance “Alicia Alonso,”—it was located in the Complutense University, where we started as pioneer studies of dance with the guide and support of his Magnificent Rector Mr. Gustavo Villapalos—we started the first investigations to adapt and to validate the Psicoballet’s Methodology to our culture with clinical applications and social intervention in different contexts. With the direct advice of Alicia Alonso, in the practical part of the procedure, and Alberto García, who guided us in the forms of theoretical work of the model as the first Cuban expert, we were mounting the Chair Alicia Alonso in the Complutense University. Simultaneously, we began the studies with a Magister based on the program of the existing studies on Cuba and, later, developing the Master with an experimental program, as we were confirming the discipline with the legal agreements of the Council of Education and the Department of Education.
In addition, a network of national institutions participating in the project was established with University of Alcalá of Henares in Madrid and with University of Castellón and Alicante. Also with the Valencian Generality that, in that time, was when the University of Valencia which had awarded a Honoris Causa Doctorate to Alicia Alonso, May 6, 1998. In this year, the University of Valencia only awarded two doctorates Honoris, the other one went to the historical maker of the Spanish transition to the democracy Mr. Adolfo Suárez Gonzalez. It was there, in the preparatory ones of her appointment like Doctorate Honoris where, in very emotive conversations, we took the initiative to start working toward a legal validation of the Psicoballet’s Cuban Methodology in Europe, but that process is not yet complete.
From these dates, different historical dancers of the National Ballet of Cuba, who have been exercising the teaching in our Institution and who have been occupying prominent positions in the international world of art, showed us procedures experienced for them in the National Ballet of Cuba. Not only they were showing to the world the ballet company, the most virtuous and universal global acclaimed, they also realized social and clinical labor in the shape of cultural enrichment and social positive action to raise the quality of life of the Cuban population with special needs.
Other companies have followed this example. At present, the English National Ballet, directed by the brilliant and prestigious ballerina, Dra. Tamara Rojo—“Prince of Asturias Prize” and the maximum distinction of the British Government, that realized her studies in our Institution and she took doctor degree [4] under the direction of the author of this chapter—is realizing meetings held in her headquarters in London of support to needy groups.
At that time of development of Psicoballet’s processes in Spain, we have been fortunate to have been supported by Loipa Araujo, Aurora Bosch, Marta Bosch, Mirta Pla, Lienz Chang, and Adolfo Roval; they were indispensable until we are in direct relation with Georgina Fariñas and her historical equipment who supervises, advises, and supports all our projects.
The first work and subject matter that we develop arose being the author the person in charge of the psychological direction of the Olympian Equipment of Gymnastics. One matter called us the attention, the dancers of the National Ballet of Cuba, neither had problems of corporal image nor the consequences derived from eating disorders. For this, we began an agreement of cooperation with the Royal Spanish Federation of Gymnastics that they adopted, to complete their trainings, the methodology of the Cuban School, which supposed a radical change that benefited to both institutions and that led to win a Golden Medal of the Spanish Equipment of Rhythmic in the 1996 Atlanta Olympics. Later, it applied to other disciplines obtaining also historical achievements as the First Medal of Gold of World Champion of Artistic Feminine Gymnastics and the First Olympian Medal of Artistic Feminine Gymnastics [5–7].
We realized the first intervention with 28 teenagers with problems of eating disorders that managed to overcome their problems of corporal image with the Psicoballet and with it to improve and to overcome finally their eating disorders [8–12].
These positives changes encourage us to develop later works with serious mental illness, realizing in 1989 the first process. The study was performed at the Psychiatric Hospital of the Havana, with a small group of 17 psychiatric patients where they obtained an enormous improvement of their quality of life and of a good number of symptoms.
In 1990, we began the first interventions of support to groups of elder people generating a system of intervention in psycho‐geriatrics, a process that has taken us to the current pioneering experiences in neurodegenerative diseases, as the investigations in process that we are realizing in “CREA” in Salamanca, center of reference of the IMSERSO over Alzheimer. They show the beneficial usefulness of the dance in the processes of support to the cognitive recovery, advances already gathered in different presentations in international congresses of maximum level, and in a doctoral thesis that the author has directed [13].
The intervention with victims of violence of gender, terrorism, violation, sexual exploitation, and so on had obtained ideal results. In neurodegenerative disorders (Parkinson and Alzheimer) this method has proved to be an effective instrument to reduce the speed of the degeneracy and helps to recover cognitive plots –it has been mentioned in the works of investigation of some doctoral theses directed by the author and realized in collaboration with the IMSERSO.
We have verified its efficiency in the problems of corporal image and eating disorders, chronic diseases (human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS), and breast cancer), and disabled persons. We have realized several interventions with patients with these illnesses [14–16].
A new intervention was made with patients diagnosed of fibromyalgia [17]. We are facing here a disease that does not find an organic test to explain an intense and diffuse pain, together with a great quantity of symptoms such as sleep problems, fatigue, depressive symptoms and anxiety, morning stiffness, and irritable bowel syndrome. From the Psicoballet, the person works with the body, with the movement, and with the expression. The objective psychotherapy is through the art as, developed and practiced with patients, that the changes in the movement will produce changes on the psychic level and emotional level. They pay attention to the pain and try to make the person explore through the body and the movement and in a secure space and with other people in the same situation, which will make all communications very easy.
This study was performed with a group of 27 women diagnosed of fibromyalgia and pharmacological therapy, of a middle age of 41 years. Before beginning the process and on having finished it, several clinical interviews were conducted on them and different tests of psychological evaluation were applied: Spielberger’s STAI and Rosenberg’s Self‐Esteem Scale and POMS. They received 20 sessions of 90 min (60‐min session of Psicoballet and 30‐min session of cognitive technologies). A session lasted a week for 5 months.
The activity physics‐artistic that the Psicoballet contributes improves the quality of life of this type of patients, strengthening their capacity of communication, improving the self‐esteem and the vigor, and reducing the fatigue and the perception of the pain. The arts are a tool validated of great usefulness in therapeutic and social interventions: improve the quality of life, the self‐esteem, and the self‐confidence level, and reduce the anxiety.
Starting on our valuation of the usefulness of the ballet as therapeutic instrument, we want to mention by exposing the illustrious intellectual Cuban Alejo Carpentier in his closing speech to the IV Festival IV of Ballet of the Havana (December 9, 1976). The speech described an experience personal that Georgina Fariñas has told us often and that exemplifies the value of the Ballet as channel of communication extra‐verbal naturally. Carpentier narrated us that his friend, an anthropologist, transferred two aborigines from Amazonian jungle to Caracas. He wanted to obtain some type of communication with the aborigines. He took them to several places, as way of stimulation, but the aborigines were not interested for anything in the new civilization. Carpentier and his friend, without many hopes already to achieve the wished communication, took these men to a ballet representation, but the aborigines had the same attitude during the presentation. Already frustrated in their attempts, they left the men in the room at the hotel that they were occupying and they withdrew theirs. In the middle of the night, Carpentier and his friend listened to a few strange noises in the room of the aborigines. They observed, with great surprise, as these men, smiling, with grotesque movements, were repeating the delicate variations of the ballet that they had observed in the theater. Carpentier concludes that from this moment, the aborigines showed them receptive and then it turned out to be very easy to establish relation with these men, seemingly apathetic and insensitive. For the observed, Carpentier assures that “About our culture, established on the word, we do not think that, in certain circumstances, it turns out to be difficult and in occasions impossibly to communicate across it.”
We think that this is the case of the majority of the mental patients, in occasions, so severely upset that they can have lost the whole contact with the world that surrounds them and, without having to come to extreme cases, we know that this case is also of anyone who suffers an emotional severe alteration. Anyone who is in deep condition of worry, melancholy or depression, finds it difficult to support a conversation beyond a few minutes, but the artistic forms, with intention of communication, have a great importance for these patients immersed in intense problems.
In the interventions that we have realized with chronic diseases, the works realized with AIDS patients stand out. The process of the disease of the AIDS is complex and very little understood for the population in general. It has different phases in which they have different emotional conditions that can be attenuated by physical and artistic activity, as we have seen in the Cuban experience of using the technologies of the Psicoballet for the treatment of different ailments.
Another area that we have investigated and adapted the experiences of the Psicoballet is the intervention with children and teenagers who have been victims of sexual abuse [18]. In this experience of adjustment of the methods of Cuba to our culture, 19 children and 13 teenagers took part, where a battery of tests were applied (Self‐esteem of Rosemberg, Spielberger’s STAIC/STAI, CDS depression) before the meetings to begin Psicoballet. Two meetings took place for 6 months every week, and on having finished, 81% of the participants were showing low self‐esteem to the pretreatment. To the posttreatment, this number descends to 53%. The level of anxiety in the pretreatment was 77% and to the posttreatment it descended to 41.3%; the depressive symptoms that before initiating the treatment had an incident of 83% descended to 51.03%. In the controls after 6 months of finishing the process, 47.6% not only improved in these variables but also recovered from the point of view clinic.
The sexual infantile violence has consequences in their victims. The whole series of repercussions and adverse psychological sequela, emotional and social, puts in commitment the integral development of the person and determines a series of neurological pathologies. A meta‐analysis with articles of 22 countries showed that 7.9% of men and 19.7% of women have suffered some form of sexual abuse before 18 years [19].
The sequels of a sexual abuse committed in the infancy or adolescents studied by several authors are fear, nightmares, disorder of posttraumatic stress, depression, social withdrawal, neurosis, regressive conduct, somatic disorders, inappropriate conduct sexual, regressive social behaviors, delinquency, problems of learning and hyperactivity, disruptive conduct, or direct affectation in the development psychosocial. All this can affect in the future conduct of the person with an increase in the delinquency and the sexual problems during the adulthood.
The Psicoballet occupies a modality inside the Therapies Artistic Creative or inside of the Body‐oriented therapies and psychotherapies (BOT/BOP) [20]. It defined the use of psychotherapeutic movement inside a process that chases the psychophysical (body‐mind) of the individual. It is characterized by the use that it does of the way and artistic process (in this case, the dance and the movement) to help settle the emotional or psychological conflicts. Part of the basic premise that any corporal movement can take in turn to change in the psyche promotes the health and the personal growth: The body, its movement, its language, and its forms of expression. The Psicoballet promotes and provides a soft way to repair the damages caused by the sexual abuse, in this case, occurred in the infancy. It offers a way of approaching the memories and painful recollections with dynamics and body techniques that are less challenging for the patient, promoting a therapeutic work embodied, focused on the emotional objection through the corporality that could offer well‐being in the current and future life of the patient. The Psicoballet introduces the use psychotherapeutic of the movement and the dance as a creative form of the emotional, cognitive, and social integration, using the body and its own corporal language. In addition to suffering significant physical, psychological and emotional consequences, these victims are often limited in their ability to work and to interact day in and day out.
The Psicoballet is a very useful tool to work with these problems because facilitates the access to the body to psychiatrists and psychologists, breaking the cuirasses that block the body with the movement and the communication [21].
The Psicoballet can be defined as a therapeutic method that integrates science and art, specifically the Psychology and the Ballet of harmonic and balanced form. From these two previous systems, the Psychology and the Ballet, the Psicoballet conforms as a new dynamic integral system in which diverse elements or subsystems are interrelated: technologies and psychological methods, which use it as base, dance, music, pantomime, dramatization, physical culture, movement, and games.
Psicoballet’s method takes elements of the educational methods as the learning that is achieved in elementary technologies of ballet and dance; it is in addition a therapy of movement, where we use the action, the movement systematized inside the ballet technology, which is the instrument to realize the therapy. This forms part of the group of artistic therapies, specifically dancing, which is of great usefulness as a method psycho‐corrective, given to the rectification of the structure of the personality and of the mechanisms psycho‐corrective.
This method chases as aim the psychic and social adequacy of the patient across the correction and compensation of his disability, achieving independence, self‐confidence, self‐assessment, self‐esteem, improvement in their communication, and familiar and social interrelationship, with the results of the Psicoballet as therapy of movement.
The systematizing of the steps helps in the development of the muscular coordination, the control of movements, the sense of the space, and the rhythm. The use of the dance in the meetings of Psicoballet helps in the royal enjoyment of the activity, which is very important for a therapy; by this way, an easy and agreeable communication establishes extra‐verbal that it is not required, it does not attack, and it provides happiness and possibilities of creation.
The general aim of this work is the rehabilitation, fitting out, or reeducation of these people in search of a feeling of self‐realization, as human beings are more part of their community and of their family of society. The preventive aspect develops in addition, with the aim to anticipate possible emotional disorders. The correct position is about obtaining a correct aesthetics of the body and a socially suitable behavior. In consequence of their diseases, these patients are inclined to choose a shod position that makes them look like guilty; it is important to work on the position, a matter that the ballet technologies facilitate. This constant growth, that is required in any class of ballet, help them to get a correct placement of the body.
The process of the education is a joint activity between teacher and student who possesses a double aspect: the instruction and the education. The method and technology for the therapeutic treatment of the Psicoballet possesses a methodological program, where the elementary level of the Cuban School of Ballet is selected. This program develops in five levels. In addition, the program possesses an offer of exercises as guide for the teacher and therapist to achieve that the mental is to the physical united.
The session of Psicoballet divides in three parts:
Motivation in bar is the moment where the therapeutic exchange begins to achieve the best correction of the steps with the fastened patients of the ballet bar. Here, it is where the steps are taught for the first time and a better balance is achieved for his future development; this is where the motivation of the therapy begins for the continuity of other meetings.
The fitting out in the center is already in the center of the lounge, where the steps that have been studied in the bar by a major balance are executed. These steps have the purpose of enabling the patient to move to this new form, with the rigor and pertinent adjustments.
The creative liberation is the moment of inter‐relationship, maximum communication, of discovery, and where the equipment can analyze to the patient in certain secret messages that they find hard to transmit verbally and to use the movement to express his personality. This is where the aim of the class is valued and where they look for the acceptance and not the conformity.
Taking advantage of this benefit that the ballet brings to us is the use of it as therapy to improve, in this case, the life of the persons with HIV/AIDS improving his quality of life, raising their conditions, finding the reasons of stress, the self‐confidence, in routes to optimize, and to balance the system of defense.
A very narrow relation exists between the emotional disorders and the lack of harmony of the movements. This lack of harmony appears across a sign of discomfort because any disorder concerns equally the body and the mind, the psychological problem reflects in physical problems and vice versa [22].
An abundant number of experimental studies show that the establishment of a program of exercise consistent and prolonged in the time has the effect of increasing the self‐esteem and of reducing the anxiety. The accomplishment of exercise can eliminate the anxiety and the tension. It is verified that the program of exercises of moderate intensity has a beneficial effect on the immune system. Specifically, we found that the exercise of moderate intensity was reducing the number of days of disease. The improvement of the immune function can derive from the reduction in the stress and from the benefits of the exercise as for the reduction of the concentrations of the hormones related to the stress as the cortisol [23].
The effects of the movement imply from the decrease of the immunosuppressant up to the increase of the self‐esteem. The increase of the self‐esteem transformed into an improvement in the quality of life. As for the physical qualities, an increase is registered in the perception of the force, the resistance, the flexibility and the balance as well as also in the physical appearance and in the physical skill. All the dimensions of the physical auto-concept are transformed into strength and flexibility.
From the numerous established educational programs, it is necessary to distinguish the line of applications of the dance in situations of posttraumatic stress that we initiate immediately after the tragic events of the terrorist attack on Madrid in March 2004. The attempts of March 11, 2004, were a series of terrorist assaults in four trains of the network of Surroundings of Madrid carried out by the jihadist terrorists. It is a question of the second major attempt committed in Europe up to the date, with 10 almost simultaneous explosions in four trains to the rush hour of the morning (between 07:36 and 07:40). Later, after an attempt of deactivation, the police would detonate, of controlled form, two appliances that had not exploded, and deactivate a third party that would allow, thanks to their contents, to initiate the first inquiries that they would lead to the identification of the authors. A total of 191 people died and other 1858 were hurt. On December 17, 2004, Gregorio Peces Barba was high Commissioned for the Support to the Victims of the terrorism for the Cabinet.
The own Gregorio Peces Barba, connoisseur of the Cuban experience, put in touch with our Institution to suggest us the creation of a program of attention to the victims of the attempt. We created a system of intervention combining the artistic therapeutic activities of the dance. We used and verified Cuban experience of treatment with the Psicoballet with cognitive technologies of treatment of the posttraumatic stress, incorporating new technologies in that moment in our country as the EDMR, desensitization, and prosecution for ocular movements, a psychological therapeutic technology used to desensitize and to re‐process psychological traumas in a natural and rapid way.
We initiate the contacts with victims of the attempt and their treatments, but the political questions ended up by bringing over to the victims of gender violence. We have realized seven programs with this group and have attended more than 700 victims of this type of violence, with very beneficial results of the decrease of anxiety and depression and elevation of self‐esteem [24, 25]. To the beginning of the last course, they requested a similar intervention with victims of sexual violence; the dance is a great auxiliary tool of support to an experience who realized Psicoballet by 21 women of an average age of 32 years who had suffered violation and had symptoms of posttraumatic stress [26–32].
On having finished the procedure, the participants showed positive changes to psychological and corporal level; 37% diminished his levels of anxiety in 45% as average and those of depression and their self‐esteem increased significantly, 42%, which allows us to affirm that the dance therapy is useful to treat this type of patients.
It is slightly functional to treat patients who present experiences from sexual abuse without a specific attention of the body, question that the Psicoballet allows and that canalizes and amplifies any therapeutic process. The utilization of the art‐therapy technologies under a cognitive behavioral model of intervention is very advisable for a problematics as the treated one. The use of an instrument to mobilize the body as the dance amplifies the effect of the psychological conventional treatments, and it allows overcoming the corporal inflexibilities that this type of disorders generates in their victims. On having unified the mental work with technologies of movement that allow to liberate tensions and be aware of the corporal condition, joining this fact the positive effect of being employed at a group of persons with same and delicate problematic opens channels of communication.
The tango is a dance characterized by passionate and marked movements; generally, it is associated with the Argentine and Uruguayan culture. Though it needs concentration and agility at the same time, a study realized in Washington verified that the dance turns out to be an excellent physical therapy for the patients with Parkinson’s disease. Besides favoring physically the persons with Parkinson, the tango might be a great source of social integration that, at the same time, would improve the self‐esteem of the patients and with this one, their emotional health.
The movement alterations seen in Parkinson’s disease are one of the most important symptoms and are the more concerning the quality of life. The dance‐like artistic and therapeutic practice can help in the rehabilitation of alterations neuromuscular and motor skill. We realize an exhaustive evaluation of studies that were investigated which brings over if the dance favors the rehabilitation of the patients with Parkinson.
Styles different from dance showed favorable results in parameters such as physical function, balance, walk, risk of fall, and quality of life. In spite of few clinical tests, the analysis of the results will arise that the dance can improve the rehabilitation of motor skill alterations; it appreciates a diminution of risk of fall on having improved the balance and the walk. All this would carry a better quality of life.
The Parkinson is one of the neurodegenerative diseases more prevalent in the population of advanced age and one of the principal reasons of falls. Difficulty with walking and the balance are common between the individuals with Parkinson, contributing to a major incident of falls. In these patients, the alterations of the movement are characterized by slowness and the accomplishment of short steps dragging them for the soil with a flexed position. Haste and/or freezing of their movement can be included. They are in the habit of presenting difficulties of balance on having realized drafts and having walked backward. The works of investigation that have studied alternatives of movement across the dance in patients of Parkinson have demonstrated benefits in the neurological condition and the initiation of the movement. The Argentine tango has arisen recently as a promising approach to lessen the problems of balance and walk. It is a combination of the following steps: they imply the beginning and frequent cessation of the movement, spontaneous way changes, rhythmic variation, alternative change of center of mass of a leg to other one, and a wide range of speeds.
These characteristics can direct for him specifically the alterations motorboats associated with Parkinson’s disease, as the difficulties with the beginning of the movement, the deficiency of the length of the stride, the freezing of the walk, and the drafts and the bradykinesia that these patients suffer. The Argentine tango is a form of expression artistic and full of meaning. The music of tango believes an environment of contemplation, desire, and intellectual stimulation. Provided the attention of a dancer must be divided between the navigation and the balance, the tango helps to develop cognitive skills, as the double task. On having used the Psicoballet with a group of patients of Parkinson, we could state benefits in the quality of life and improvements in their processes of walk and major safety and self‐esteem.
To psychiatric level and of severe mental illness, we have realized several interventions; some of them in process form a part of doctoral theses in process of ending, besides the pioneering investigation mentioned with schizophrenics, and recently we finish a process with bipolar patients. The bipolar disorder, considered a mental serious disorder, named traditionally as maniacal‐depressive disease, is characterized by a changeable state of mind that fluctuates between two opposite ends: the obsession, or phase of exaltation, euphoria and grandeur, and the depression, or phase in which they predominate over the sadness, the inhibition, and the ideas of death. A chronic disease limits the functionality of the patient, needs a mixed pharmacological boarding and psychosocial. In the cases badly diagnosed and with bad orientation of treatment, the effect in the disease is devastating and implies important economic loads and socio‐sanitary.
Often, the results for the patients with bipolar disorder where they are treated with medicament therapy only are suboptimal. The evidences suggest that the exercise is an adjuvant treatment psychosocial for the treatment of these patients. The exercise increases the aptitude to adapt to stressful environmental and it might reduce scoreboards of allostatic load reducing the activity of the axis hypothalamic‐pituitary‐adrenal (HPA), the sympathetic nervous system, and the corticosteroids. The experiences of the Havana’s Psychiatric Hospital with the introduction of the Psicoballet in these problematic complement the effects of the physical exercise on having included, plus the own exercise, adjuvant therapy of communication, accomplishment, and an increase of the self‐esteem.
An experience was realized with a group of 21 adults of bipolar disorder applying Psicoballet’s Cuban Methodology—the same way as carried out at the Havana’s Psychiatric Hospital—to study the effects with patients, of our culture, to whom a follow‐up was realized with clinical interviews [33].
The dance as physical exercise has the advantage of that the effort can graduate and adapt to the needs of every participant, without demanding a physical effort that fatigues and demotivates the people. The dance increases the communication and turns into a motive of expansion into the day that helps to the treatment.
This original therapeutic production, which had his historical origin in Cuba and which has spread over numerous countries of America, Europe, and Asia, was recognized by the UNESCO. We have studied and adapted, in our University in Madrid, Spain, the original Cuban methods to our culture and have studied their effects in different groups of disease and contexts, finding an undoubted usefulness of support for the clinical evolution of some disorders and in the evolution of the quality of life of the patients who suffer these problematics [34–37].
Psychology and Psychiatry have forgotten the body. The psychologists and the psychiatrists work on mind, thoughts, emotions and behaviors but they do not observe body, nor intervene on it, but art therapy is a way to get back to the body.
The patients work with the body and with the movement in company of other persons who have the same problems, traumas, and emotions with similar blockades. They enter a spontaneous communication of their problems that favor the work in groups, and moving and using the body as part of the therapeutic process, they liberate the blockades and inflexibilities shooting the efficiency of the psychological treatment.
The great contribution of this group of artists and clinical Cuban is an important advance to raise the quality of life of the patients for the effects that the art has in the brain: the music, the dance, the dramatization, and the singing [38].
Clinical meetings with psychologists, psychiatrists, doctors and professional artists to plan and to supervise the processes of treatment take place at all time.
We can conclude that the number of clinical tests with a suitable randomization and methodologically rigorous is not numerous in the international bibliography, being necessary to increase the investigation in this matter in order to obtain clear conclusions.
Nevertheless, several studies coincide in indicating the efficiency of the art therapy in the reduction of the negative symptomatology in mental illnesses as the schizophrenia. In this respect, there are promising results referred to the dance therapy and to the corporal psychotherapy, being, of between all the modalities, the most robust evidence for the music therapy. With regard to other variables, the superiority of the art therapy could not have demonstrated opposite to standards care, and though the music therapy has presented positive effects on the mental general condition or the social functioning, these are not considered in all the studies.
In September, 2008, the National Institute for Clinical Excellence National (NICE) published a document “Draft Full Guideline for Consultation” that dedicates a few pages to the expressive therapies and to the scientific evidence that has been demonstrated in the last studies of investigation. This study concludes that the principal benefit of the application of artistic therapies is the improvement in the negative symptomatology of people with psychosis. In the same document, details that the person who guides or executes the abovementioned practices must possess a specific formation in the matter.
In 2009, this Institute included a paragraph which mentioned the Artistic Therapies including them inside the “Psychosocial Interventions.” NICE recommends prescribing artistic therapies such as art therapy, dance movement therapy, corporal therapy, drama therapy, and music therapy as complement to the psychotherapy and to the pharmacotherapy.
The Cochrane Library has published different studies which have an effect on exhaustive review of articles that evaluate the art therapy as adjuvant treatment for the mental illnesses in comparison with the standard treatment and other psychosocial treatments. In its introduction quote “The British Association of Art Therapists (BAAT) [39] defines Art Therapy as: Art Therapy is the use of art materials for self‐expression and reflection in the presence of a trained art therapist. Clients who are referred to an art therapist need not have previous experience or skill in art, the art therapist is not primarily concerned with making an aesthetic or diagnostic assessment of the client’s image. The overall aim of its practitioners is to enable a client to effect change and growth on a personal level through the use of art materials in a safe and facilitating environment.”
The arts have a clear future in the clinical, social, and educational applications, and their utilization, consolidated in some pioneering countries in the matter as Australia, Cuba, Canada, The United States of America, the United Kingdom, and Israel, is universalizing according to evidence confirmed of their utilization and attested by a certificate by transnational organizations as the UNESCO.
The spread of non-native species and their impact on the environment are a much-noticed topic in science and nature conservation. Recently, also a broader public is becoming increasingly interested, especially as the annual economic loss caused by alien species is estimated to be up to 5% of the world economic output [1]. Moreover, invasive alien species are an important factor in the loss of biodiversity. In fact, an analysis of the IUCN Red List shows that it is one of the most common threats associated with extinct species. Invasive alien species can also lead to changes in the structure and composition of ecosystems that have a significant negative impact on ecosystem services and affect the economy and well-being of humans [2, 3, 4]. Although the number of documented invasive species is underestimated in many countries, the introduction of invasive species has increased significantly. In Europe, for example, the number of invasive alien species increased by 76% between 1970 and 2007 (IUCN). Only a few of the thousands of species introduced into new areas actually become invasive, which is why their identification is the main objective of invasion biology. In Austria, 1110 alien vascular plant species have been identified, which account for 27% of the total Austrian flora. Of these, 17 species are problematic for nature conservation as they invade near-natural habitats [5]. Japanese knotweed, Fallopia japonica, is one of them and is considered to cause large changes to the communities and ecosystems it invades. Its large size and its clonal, monocultural growth lead to the visual, structural, and chemical transformation of ecosystems. Wherever the plant takes root, the diversity of plant species decreases. The remaining competing species are mostly non-native [6, 7, 8, 9] and show strong reductions in height, biomass, and specific leaf area (SLA) [10]. Once a F. japonica stand is established, the clonal connectivity increases its ability to grow further [6]. The vast spreading in riparian areas also results in the reduction of an overall abundance of invertebrates [3, 7]. Therefore, a large-scale invasion of Fallopia species is likely to seriously affect the biodiversity and quality of ecosystems and should be prevented [7].
Not only does Japanese knotweed have a negative effect on the environment, but it also causes damage to infrastructure and costs effort and money for removal work. Each year, a considerable sum is spent on vegetation management on railway and road networks [11]. Fallopia japonica prefers manmade locations where other plants do not have a chance; in railway structures these are graveled areas, platforms, and loading areas. Weed control is primarily carried out in the track area in order to avoid fine soil and humus accumulation and thus reduce increased water retention capacity. Also, for the treatment of the track-accompanying paths, security is the main reason [12]. The urgent need for action can also be seen in our current projects: the project “Vegetation control on roads and railways” aims for vegetation control of traffic infrastructure areas with a balanced consideration between conventional and effective eco-alternative methods. In another project we are taking over the scientific monitoring for railway embankment grazing on the Koralm railway in order to control Japanese knotweed [13].
In agriculture, in addition to knotweed competing with crops, contaminated goods such as humus landfills pose a real problem. Open soils and disturbed vegetation provide an opportunity for problematic plants to colonize. One centimeter of root is enough for Japanese knotweed to form a new population [14]. According to Section 21 of the Carinthian Nature Conservation Act, the release or sowing of wild plants […] into areas in which they are not native requires a permit. A permit may only be granted if neither the natural habitats nor the native wild animal and plant species are damaged. Large economic losses can therefore occur if humus landfills partly or fully overgrown with knotweed can no longer be used as such.
At present, there is no fully effective method to control knotweed. Still, in the literature, there is a long list of control methods ranging from mechanical methods such as pulling out and mowing [15] to grazing with sheep and goats [16], planting competitive native species [17, 18], covering the roots with tarpaulin, and using herbicides [18, 19] to biological control such as the use of Japanese knotweed psyllid [20].
In summary, characteristics, effects, and control measures of Japanese knotweed are subject to numerous research projects in Central Europe and North America; “Game of Clones” is one of them and approaches the topic in a somewhat different and playful way. A team of scientists together with the help of high school students aims to spatially model the spreading behavior of knotweed under different circumstances and to create and provide a computer simulation as an experimental platform as well as a board game. Considering that multiple components are required, first, a vast understanding of knotweed, especially regarding its ecological optima, its dispersal strategy, and its response to different control measures, is necessary. Therefrom, sustainable assumptions can be developed to be able to model the responses of knotweed to each control measure. For answering some of the questions, experiments will be used. The outcomes will lead to the creation of a board game and a computer simulation model based on a cellular automaton to be able to analyze and demonstrate the spreading behavior of knotweed in an interactive manner. Players will try out different measures to eradicate the clones and to keep particularly valuable areas clear from the weed. Doing this, they should go as easy on resources as possible. Depending on the individual starting points, different measures and combinations of measures will lead to success, in other words, reduce or stop the plant growth. The game takes place on actual existing land (satellite images), so the computer simulation can also be consulted for concrete action planning. The students in the research project will also play a part in the browser-based programming of the strategy game; in this way, they will simultaneously be an important reference group regarding its user-friendliness and functionality. The present chapter covers the results of the research activities and experiments within the project and gives a comprehensive review about Japanese knotweed. Section 2 starts with a description of all methods used, and Section 3 will be about the corresponding results. Section 4 finally discusses the question of the necessity of invasive species removal, summarizes the results, and concludes with a range of further recommendations for improving the existing evaluation and monitoring frameworks.
In “Game of Clones”, a multitude of methods have been and are used. This is especially important because it is the only way to fully understand Japanese knotweed in all its parts and behaviors. The following chapter will describe each method with all its limitations and challenges in detail to be able to relate to the results.
As a start, the team of researchers has carried out an extensive literature search. The contributions and articles collected were reviewed and classified as more or less relevant to the research question of the project. With the support of the Regional Museum of Carinthia (Landesmuseum Kärnten), a bibliography of over 200 relevant papers on Fallopia japonica was compiled and divided into various topics: classification, taxonomy, identification, characteristics, history, growth, reproduction, spreading, usage, impacts, monitoring, control, management, invasions, and modeling.
For a serious discussion about the plant, the most urgent question that needs to be clarified and cannot be answered by the literature is what exact species we are dealing with in our project area. In Central Europe, there is evidence for two introduced species, Fallopia japonica and Fallopia sachalinensis; their hybrid Fallopia × bohemica has begun spreading as well [6, 21, 22]. The two original species are relatively easy to distinguish based on the shape and size of their leaves, but discriminating them from hybrids is challenging, even for experts. Hence, we will make use of DNA-barcoding, a taxonomic method for species identification using the DNA sequence of a marker gene [23]. The sequence of base pairs is used as a marker for a particular species, analogous to the barcode on food packaging. Since the DNA sequence changes by point mutations at a generally uniform rate, more closely related individuals (and species) have more similar sequences. As long as a species remains undivided, i.e., has a common gene pool, differences between different populations are compensated again and again by gene flow. So, if samples from two individuals have clearly different sequences, this is a sign that they come from different species [24]. The analysis of two marker genes (chloroplast marker and nuclear marker) should provide information on hybridization and distribution of the species in our project area of the Austrian federal states of Carinthia and Styria. The chloroplast marker is inherited from the maternal organism, so by using it we will see what species was maternal. The nuclear marker will indicate if the plant is homo- or heterozygote, therefore a hybrid.
In the months of July and August 2018, 95 leaf samples were collected and sent to the Canadian Centre for DNA Barcoding (CCDB) in Guelph for DNA sequencing. For 3 weeks, 72 of the leaf samples were taken from individuals in Carinthia and Styria. Care was taken to ensure that different locations and morphologically different stands were chosen. If a site was selected, a tissue piece with an area of 1 × 0.5 cm was sampled with clean forceps. Preference was always given to the youngest and greenest parts of the plant, rich in plastids and meristematic cells such as the tip of a leaf. The samples were then placed in airtight bags of silica gel and kept to dry. Before proceeding to the next sample, it was crucial to ensure that no residual tissue remained on the forceps by rinsing them in 95% ethanol and wiping them with a clean absorbent paper. For each sample, a herbarium voucher of several leaves and flowers was collected, dried, and archived in the Regional Museum of Carinthia. Additional metadata included the assumed species, age, and sex as well as a detailed description of the site consisting of GPS coordinates, address, and site conditions. A photo documentation comprising location, entire plant, leaf surface and underside, and flower complements the sample collection (Figures 1 and 2).
Required tools for field sampling (E.C.O Institute of Ecology).
Herbarium voucher of a sample of Fallopia sachalinensis (Herbarium collection code: KL—Kärntner Landesherbar).
Each sample was assigned to a Museum ID, which links it to the voucher, the metadata, and the photo documentation. A total of 13 of the 95 samples were collected from reliably identified individuals of all three species from the herbarium in the Regional Museum of Carinthia to serve as a reference. Ten of the 95 samples were not taken from the field, but CCDB offered to organize reference samples from Eastern Asia to have some samples from Japanese knotweed’s native range.
The analysis is still ongoing; in case the sequencing will be successful, the data will be fed into the global Barcode of Life Data System (BOLD).
Growth rates and propagation patterns are crucial parts of the basic data needed for the modeling of Fallopia populations. That is why we set up two transects on the campus of the Lakeside Science & Technology Park, a science and technology park in Klagenfurt. A transect is a straight line along which one counts and records occurrences of a species. The main advantage of transect mapping is its repeatability and standardization even under difficult terrain conditions. Both our transects (10 m each) were border on infiltration areas. The exact position of the transects was chosen in such a way that the shoots are rather in the middle of the observation area in order to be able to measure the propagation better. All methods were implemented according to the manual of vegetation-ecological monitoring by Andreas Traxler [25]. The transects were divided into 10 subplots (1 × 1 m each); the measured plants were each marked with a piece of yarn. In a weekly monitoring (April–July), the growth and propagation of Fallopia japonica was observed with two methods. On the one hand, three shoots were selected in both transects, in which the shoot width (at a height of 10 cm) and the height itself were measured with a caliper and meterstick. On the other hand, the number of shoots in each subplot was counted, and new shoots were marked and measured for their exact position. The data gain significance if they are interpreted in connection with the weather data for the period in question, as it is intended for the compilation of the logarithms of the computer simulation.
A good understanding of the underground processes in the Fallopia clone is of central importance for our research. To understand the connection between plant growth above- and underground and the knotweed’s reaction to obstacles, we laid bare the entire root network of two stands in a large-scale field experiment. The method we used was already developed and successfully applied for the root exposure of forest trees in the past [26].
The stands are located on the campus of the Lakeside Science & Technology Park in Carinthia that borders directly on the Natura 2000 site Lendspitz-Maiernigg. During construction works 2 years ago, building rubble was piled up and populations of Japanese knotweed were able to colonize the area. The first location is a 4 m high hill with a 2-year-old stand; the second location borders on the parking lot, and its stands already exist for 4 years.
After the excavation work had been carried out and the site on the hill and next to the parking lot had been dug down by 2 m, the manual excavation work began. Together with the students, teachers, and soil experts of our two cooperating schools “BORG Spittal” based in Carinthia and “HBLFA Raumberg-Gumpenstein” based in Styria, the roots were then uncovered in a period of 2 days (Figures 3 and 4). The rough work was done with shovels, spades, and picks; the fine work was mainly done with screwdrivers. Bit by bit, the earth was dug away along the rhizomes and roots, thus exposing the roots. The results were documented in writing, in photographically, and in overview and detail drawings. The excavated shoot and rhizome parts were disposed of by the waste management department of the city of Klagenfurt so as not to contaminate further soil. After the two work days, the holes were dug up again by the excavator.
Excavation work at location 1 (E.C.O. Institute of Ecology).
Measuring the length growth of the rhizome (HBLFA Raumberg-Gumpenstein).
Rhizoboxes are a non-invasive investigation method, which offers the possibility to survey the root system growth dynamics in time and space. Based on the root uncovering in Carinthia and the knowledge gained about length and width growth of the underground biomass, the experimental arrangements for the rhizoboxes were proposed. After a test experiment, adaptations took place; further experiments will follow. The method of using rhizoboxes aims to answer the following questions: how quickly do the rhizomes of knotweed grow (growth rates and depth and width growth) in vertical and horizontal rhizoboxes? What are the limiting factors (e.g., aboveground biomass, drought, cold, light, etc.)? For this purpose, 10 rhizoboxes in size of 30 × 100 cm were built, five in horizontal and five in vertical alignment (Figures 5 and 6).
The earth material must be sieved before the boxes are filled (HBLFA Raumberg-Gumpenstein).
The rhizome was traced to simplify the measuring (HBLFA Raumberg-Gumpenstein).
Attempt 1: For the first experiment, fresh rhizome mass of Japanese knotweed was used to illustrate the growth in length, height, and width. On 13 July 2018, the first two boxes were filled with fresh earth material and the rhizome mass was planted (box 1 = 4 cm piece and box 2 = 7 cm piece). The first attempt was aborted because of glass jumps, mold formation, and too much soil and water.
Attempt 2: The second attempt started on July 27, 2018; four boxes were filled with fresh earth material. The length growth of the rhizome was measured approx. every 3–4 days, and the growth spurts were documented in an Excel file. The alignment of the boxes was optimized; the rhizome parts cast less intensively. After about 3 weeks, the growth directions and lengths were traced with a white marker. The boxes are still filled, and the rhizomes are moving inwards. Next steps will include:
leaving some earth and rhizome material in the boxes and storing it over the winter (covered with fleece) in order to test whether there will be further growth next year,
experimenting with two boxes being filled and sampled and simulating a longer growing period in the boarding school at a nearly constant temperature—the results are then evaluated in spring 2019, and
starting a new rhizobox experiment with fresh material in spring 2019 and precisely documenting length and width growth.
The rhizobox experiments were all conducted by the students Philipp Poier and Julian Heywood and their teachers Renate Mayer and Irene Sölkner from the HBLFA Raumberg-Gumpenstein. Next year, observations will be longer and more regular. The present studies served as first pilot experiments to get familiar with the method.
All experiences gained in our research will influence and have influenced the development of both the analog and the digital version of the strategy game ‘Game of Clones’. The game is based on a spatial model using cellular automatons (Figure 7) to display dynamic vegetation patterns [27]. The basic approach of cellular automatons is a subdivision of the area into equally sized, mostly quadratic fields. The dynamics of the modeling results from an interaction between the neighboring cells, in which a “state” is set to overlap the neighboring field (discretely modeled temporal development). When defining neighborhoods for quadratic cells, the van Neumann neighborhood and the Moore neighborhood are being distinguished. Whereas in the van Neumann neighborhood only cells with common edges are considered neighbors (this results in 4 neighbors per cell), the Moore neighborhood also defines diagonally adjacent cells as neighbors (this results in 8 neighbors per cell). For “Game of Clones”, we chose the approach of a model with hexagonal cells, in which there are always exactly six neighbors. An application of a hexagonal model can be found, for example, in the SCIDDICA model, which models the behavior of landslides during strong water accumulations [28].
Principle of the cellular automaton with hexagonal cells (Institute of Networked and Embedded Systems, University of Klagenfurt).
The modeling of the game includes biological parameters such as nutrient uptake, growth, and propagation rates as well as system parameters such as the shape and size of the cells to be simulated. This intersection of disciplines requires a close cooperation between expert biologists and modelers. Starting from the literature and empirical findings (reference area and experiments), the model is developed in an iterative process.
To be able to run the model with as many systems as possible, a browser-based implementation using html5 is provided to make the system compatible. Html5 supports the execution on operating systems and is—with certain restrictions with regard to screen size—also suitable for mobile devices. NetLogo, a multi-agent programming language with an integrated modeling environment, will be used for the simulation. The development process requires a repeated feedback of the results with biologists, whereby the model parameters and assumptions are repeatedly adjusted and compared with available findings (literature and experiments). This process is of particular scientific interest and value. The user interface is developed at the same time as the model is created. For this purpose, early user tests prototypes of the user interfaces to ensure ease of operation and an attractive design. The separation of model, view, and controller (Model-View-Controller Design Paradigm) allows a largely independent further development of program parts and supports a later independent use for other projects. The software is developed under an open source license and made available as a project result.
The board game “Game of Clones” is the analog version of the computer simulation and focuses on playability and fun instead of enforcing fully realistic scenarios. In the cooperative game, players work together in order to compete against Japanese knotweed, either winning or losing as a group. The board game was developed during biweekly meetings of the experts of the E.C.O. Institute of Ecology and the Institute of Networked and Embedded Systems from the University of Klagenfurt. A prototype of the board game is already available. During the development process, the students of BORG Spittal played through several test rounds and made a strong contribution to improving the game. The computer simulation will be completed by October 2019. In contrast to the board game, full attention will be paid to the closeness to reality whereby the program will be filled with all recorded data.
Fallopia japonica (Houtt.) from the knotweed family (Polygonaceae) has a number of synonyms, which makes literature research more difficult (frequently: Polygonum cuspidatum (Sieb. & Zucc.), Reynoutria japonica (Houtt.), and Polygonum japonicum (Meissn.)) as well as a number of phenotypically similar species and hybrids (in Austria in particular: F. sachalinensis and F. × bohemica) [29, 30, 31]. Until the definitive identification of the species in our study area, we use Fallopia japonica as the provisional collective name for these species.
Screening the literature, one of the main findings was that considering that it is only one single species, there is a huge amount of papers that revolve around Japanese knotweed. The articles cover various aspects of the plant, having a focus on morphology, systematics, spread, and control. Following a brief story-time about knotweed’s introduction into Europe, this subchapter will be about the findings which proved to be relevant for the project.
Fallopia japonica was first introduced to Europe in 1825 by Philipp von Siebold, a Bavarian physician who worked for the Dutch government in Japan. Von Siebold had a strong interest in botany and natural history and sent a large shipment of live plants—over 500 different species—from Japan to the Netherlands, one of them being Japanese knotweed (under the name Polygonum sieboldii). It was intended to make a career as an ornamental and cattle feed plant and to be used in forestry as a feeding ground for red deer and as a covering plant for pheasants. The career as a useful plant did not start so well: it is of little use as a cover for pheasants, since it loses its leaves in winter and red deer do not eat it, neither our grazing livestock. However, since in early autumn it is an excellent bee pasture when most of the European native plants have already flowered, the beekeepers have discovered Japanese knotweed for themselves [32]. Although the German Federal Nature Conservation Act and most of the Austrian Federal State Conservation Acts prohibit the planting of alien plants in the wild, beekeepers generously distributed the Japanese knotweed in the area—a first step on the way to a spread that currently places Japanese knotweed at No. 37 in the “Global Invasive Species Database”, a database managed by the Invasive Species Specialist Group (ISSG) of the IUCN Species Survival Commission.
When Japanese knotweed got introduced into Europe, they only introduced a female (male sterile) individuum, never the male indiviuum. A significant proportion of knotweed in Central Europe is not F. japonica, but the hybrid between it and F. sachalinensis—F. bohemica. This hybrid can reproduce with either parent and thus can replace the missing male specimens of F. japonica. In the same process, the hybrid produces the genetic diversity that F. japonica lacks so strikingly [33, 34].
All Fallopia species have a strong clonal growth, which allows them to surpass the surrounding species as well as to colonize new areas quickly. The basic unit of the rhizome system is a shoot clump that varies in size in different Fallopia species. In general, the apex of a rhizome branch eventually becomes an aerial shoot. When the shoot clump no longer produces new aerial shoots and dies, some lateral buds break the dormancy and begin to grow horizontally as new rhizome branches sometimes extending over 1 m. While F. japonica has rather large shoot clumps connected by long thin rhizomes, F. sachalinensis produces smaller shoot clumps that are more closely connected and grow in rows. F. × bohemica combines the characteristics of both parents and has an intermediate patch structure with smaller shoot clumps than F. japonica and longer rhizome connections between individual shoot clumps than F. sachalinensis. The fragmentation and spread of rhizomes by flooding or human activity are the most important means of propagation, as rhizome fragments of 1 cm length and 0.7 g weight can regenerate. Fallopia species can also regenerate from stem parts, but with lower regeneration rates. F. × bohemica had the highest regeneration rate of all taxa (61%) and is the most successful in regenerating and establishing new shoots. F. japonica and F. sachalinensis show lower regeneration rates (39 and 21%, respectively) [35].
The ability to regenerate in very poor soils with low nutrient requirements allows the plant to occur in a variety of habitats. It is not unusual for F. japonica to grow at the foot of buildings or on concrete surfaces [36]. The plant achieves it competitive superiority primarily by limiting access to light [37]. A factor, Japanese knotweed is very sensitive to, is frost. The plant is exposed to significant damages by late spring frosts when the shoots appear and by early frosts in autumn when the leaves senesce at the end of the growing season. This situation suggests that minimum spring temperatures may limit its range expansion [38]. However, climate change will open up habitats within threshold values, and frost conditions in these areas will be less severe and restrictive [39].
All these circumstances and many more make it extremely hard to get control over the invasive species. In the literature, there are many control methods and attempts described, but there are none that are completely convincing, and it amounts to a combination of different methods. Mechanical regulations focus on mowing, and although mowing during the vegetation period reduces the height and the diameter growth of shoots, the total weight of the biomass more or less stays the same [15]. The combination of cutting or mowing and using glyphosate has shown to be the most efficient and least time-consuming strategy so far [19, 40]. It is important to replant the area immediately with competitive native species to fight against invasive recolonization. On average, a suppression of knotweed is necessary for 2 years, before native species can be successfully established [18]. Another option is a long-term grazing of cows, sheep, or goats to keep the area knotweed-free [16]. A strategy which is becoming more popular is biological control, not least since Japanese knotweed was introduced without all its natural enemies. Aphalara itadori (itadori being the Japanese word for Fallopia japonica), a species of psyllid from Japan which feeds on Japanese knotweed, is the subject of an application for release into the wild in Great Britain. It has been licensed by the UK Government for the biological control of Japanese knotweed in England; this is the first time that biological control of a weed has been sanctioned in the European Union [41]. Other biological controls include a leaf beetle, Gallerucida bifasciata [42] or snails, Succinea putris, and Urticicola umbrosus [43].
So far, the leaf samples have been sent to the Canadian Centre for DNA Barcoding (CCDB), the analysis is not yet complete though. The expected results should clarify which Fallopia species occur in Carinthia and Styria and in what proportion. The phenotypic determination suggests that only sporadic samples of F. sachalinensis are expected. Due to insufficient morphological differences, the phenotypic discrimination between F. japonica and F. x bohemica was not possible.
The weekly monitoring of the two transects provided information on growth rates in height and diameter. The average height growth of the six plants studied decreased as the vegetation period progressed. While the growth of the shoots in April was averagely 21.7 cm per week, it dropped to an average of 14 cm in May and to an average of 1.2 cm in June. As a percentage, the plants grew by 40, 1, 15, and 0.9%, respectively. In Figure 8, the growth rate is visualized for each individual, and the initial length of the shoots is as follows: 117.6 cm (A.4.1.), 35 cm (A.5.1.), 77.5 cm (A.6.1.), 32 cm (B.6.1.), 26 cm (B.9.1.), and 36.7 cm (B.9.1.). The average diameter growth was between 1.2 and−0.5 mm per week. The negative values result from the fact that knotweed does not have woody shoots and the diameter size depends strongly on the water balance of the plant. Thus, it can happen that the diameter shrinks temporarily. While the growth of the diameter in April was averagely 0.3 mm (5%) per week, the growth rose to 1.8 mm (17%) in May. The plants lost biomass in the end of May/beginning of June resulting in negative values of −0.25 mm (−33%) and recovered in June with 1 mm (13%) growth rate. The initial diameters of the shoots as visualized in Figure 8 are the following: 12 mm (A.4.1.), 2.1 mm (A.5.1.), 8.5 mm (A.6.1.), 3 mm (B.6.1.), 3 mm (B.9.1.), and 6 mm (B.9.1.). B.9.1. has low values starting in May; this results from a sudden wind break in the shoot, which has shortened the shoot and weakened the plant.
Height and diameter growth of shoots in two transects (A&B).
The rhizome uncovering could not confirm the assumption that the largest biomass of Japanese knotweed is underground. At site 1, the 2-year-old stand grew at a height of 4 m—the longest rhizomes reached a depth of 80 cm and were mainly horizontal. The reason to assume is that the plant mainly invests in the above-ground mass in the first few years. Site 2, a 4-year-old stand, underlines this assumption. The rhizomes reach 2 m into the deep until they stand at the groundwater body, which is generally high in Klagenfurt.
The initial length of the rhizome pieces put in the rhizoboxes ranges from 5 to 24 cm. The results show that there is no correlation between initial rhizome length and growth rate. The boxes have been positioned horizontally and vertically, which showed a slight advantage for the rhizomes put in the horizontal boxes. All rhizomes started growing at a slow pace and speeded up at the end. These preliminary results have been conducted by students; further experiments are planned (Figures 9–11) and (Table 1).
Overview drawing Site 1 (E.C.O. Institute of Ecology).
Detail drawing of a rhizome (E.C.O. Institute of Ecology).
Board game version of “Game of Clones”.
Date | Rhizobox 1 | Rhizobox 2 | Rhizobox 3 | Rhizobox 4 |
---|---|---|---|---|
27 July 2018 | 5 | 5 | 24 | 4 |
30 July 2018 | 6 | 7 | 27 | 5.5 |
2 August 2018 | 6 | 7.5 | 30.5 | 5.5 |
6 August 2018 | 6 | 7.5 | 31 | 5.5 |
10 August 2018 | 7 | 8.5 | 31.5 | 6.5 |
31 August 2018 | 13.5 | 15.5 | 42.5 | 9.5 |
Rhizome length growth in rhizoboxes (rhizome pieces in cm); rhizoboxes 1 and 2 are positioned horizontally, and rhizoboxes 3 and 4 are positioned vertically (HBLFA Raumberg-Gumpenstein).
All data and experiences gained in the project result in the development of a computer simulation. Along the way, we also created a board game for children and adults from the age of 10 years, which is currently on its way to a game publisher. The cooperation game allows players to work together as teammates against the opponent, Japanese knotweed. The game starts with a landscape full of differently suitable habitats for knotweed, occupied by randomly distributed Fallopia clones. The players will try out different measures to eradicate the clones and to keep particularly valuable areas clear from the weed. Doing this, they should go as easy on resources as possible. The player team wins if they manage to displace all plants from the game plan and loses if one of the nature conservation areas is overgrown or destroyed by clones of knotweed. The game is based on event and action cards. Each round starts with an event card, meaning Japanese knotweed moves in a specific speed and a specific spreading mechanism. Then, it is the players’ turn and they can choose between action cards that portray control methods such as mowing, pulling out, sheep grazing, glyphosate, cover foil, or biological control (Aphalara itadori). Depending on the individual starting points, different measures and combinations of measures will lead to success, i.e., reduce or stop the plant growth.
In several test rounds, we could see that the players started to realize how fast and determined Japanese knotweed can spread and how little can be done about it, if one does not take it seriously. The only way is to cooperate, to combine control measures and to act as soon as possible. Whenever the population is little, it is still quite easy to get rid of, and once the board is mostly overgrown by knotweed, it is extremely hard to push back the plant. The game is designed close to reality, and in terms of controlling knotweed, it shows that mechanical methods are time-consuming and inefficient, and that poison and cover foils are more efficient, but that they are not resource-saving and that one has to live with the consequences. Thus, Game of Clones creates awareness of invasive species in a playful way. The digital version is still in process, and the students in the research project will also play a part in the browser-based programming; in this way, they will simultaneously be an important reference group regarding its user-friendliness and functionality.
(E.C.O. Institute of Ecology, Institute of Networked and Embedded Systems at the University of Klagenfurt)
To our knowledge, the presented game is the only board game applying a cellular automata model to depict the spread of invasive plant species. While modeling plant growth with cellular automata is a well-established approach (a good overview can be found in [27]), there are only a few examples for usage of cellular automata simulations in board games: Franzel describes the usage of a board game to assess farmers’ preferences among alternative agricultural technologies in [44]. Kang et al. [45] depict a computer simulation model addressing evolutionary game theory within a five-species jungle game, which is based on a Chinese board game. The work most related to our approach is the board game “Alien Invaders!” which teaches students how introduced species can affect native species with the example of native birds being affected by introduced species [46].
The project at the time of this publication still has a duration of 1 year, which means that many of the results are not yet complete and require further research.
The current results show that the issue of Japanese knotweed is very complex, and numerous studies and research projects have already been carried out and many are still ongoing. Due to the complexity and the costs of the control, the question arises whether the extensive control of knotweed is really necessary. But it turns out that even if one is of the opinion not to additionally intervene in the ecosystem and let nature take its course, Fallopia japonica also has significant economic effects, which cannot be ignored [1]. The need for further studies also arises from several disagreements in the literature such as the one regarding the gender of the species. Some say that the plant is clearly dioecious with distinct male and female individual organisms, and others speak of the plant being gynodioecious, which is the existence of male sterile and hermaphrodite individuals. There is not so much literature on the underground growth of Japanese knotweed. Hence the root uncovering was very informative, which is exactly why it would be advantageous to uncover the roots at another site, especially with older stands in order to present comparisons and observe the underground growth after the initial years.
The one method to fight knotweed does not exist. For every area, every situation, and every circumstance, a different strategy makes sense and mostly only the combination of different methods achieves an impact. When combating invasive species, however, one must always think in years. The computer simulation “Game of Clones” will be able to be underlaid with satellite images in order to establish a relationship to real areas. On the basis of the simulation, control strategies can be considered in advance of a measure concept. The project will also result in a practical guide and an explanatory video because the best way to combat an invasion is prevention and environmental education. During the collection of samples, there were numerous encounters with neighbors who were not aware of the problem and planted Japanese knotweed as a screen or threw plant remains into the compost. We hope that as many people as possible can be picked up by the game and sensitized to this topic. It would also be interesting to test whether the cooperative game method could be applied to other invasive plants in an adapted form.
This research project is part of Sparkling Science, a research program funded by the Austrian Federal Ministry of Education, Science and Research (BMWFW), a program that supports projects in which pupils of all levels of education are actively involved in the research process.
In the case of our project, students aged between 14 and 18 from BORG Spittal—a grammar school with special emphasis on music, art, sport, and science in Carinthia—supported us in the research project. Hence, special thanks to the students who helped with the sample collection for the DNA barcoding, with uncovering the root network, and with the transect monitoring and who also significantly contributed to improving the strategy game through test games and feedback rounds.
Special thanks also to our project partner DI Arthur Pitman from the Institute of Networked and Embedded Systems at the University of Klagenfurt. He was mainly responsible for co-developing the rules of the strategy game, processing the data gained in the project, and programming and modeling the computer simulation.
We express our warm thanks to the students Philipp Poier and Julian Heywood and the teachers and experts Dr. Andreas Bohner, DI Renate Mayer, and Irene Sölkner from the HBLFA Raumberg-Gumpenstein, a higher technical education and research institute in Styria that participated as a project partner. The school supported the rhizome uncovering and fully overtook the work of the rhizoboxes. Additionally, both students conducted their pre-scientific work on Japanese knotweed; within this framework, they carried out further experiments.
A big thank you also to Lisa Schmied, E.C.O. Institute of Ecology, who, during the rhizome uncovering, drew the rhizome of Japanese knotweed in all its perspectives.
We would also like to show our gratitude to our final project partner, Dr. Roland Eberwein from the Regional Museum of Carinthia (Kärntner Landesmuseum) who has been instrumental in researching the literature and who is responsible for archiving the herbarium vouchers from the DNA Barcoding.
Finally, many thanks to the Lakeside Science & Technology Park for supporting us and letting us set up transects and carry out a large rhizome uncovering on their property.
The Authors declare that there is no conflict of interest.
This is a brief overview of the main steps involved in publishing with IntechOpen Compacts, Monographs and Edited Books. Once you submit your proposal you will be appointed a Author Service Manager who will be your single point of contact and lead you through all the described steps below.
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