Global chemical reactivity parameters (IP and EA), energy levels (HOMO/LUMO), and energy gap (HOMO‐LUMO) in the gaseous phase, and methanol as a solvent for all B3LYP/6‐31+G(d,p)‐analyzed carotenoids.
This chapter is about a theoretical study applied to six carotenoids present in vegetables containing carotenes and xanthophylls. Electronic properties are analyzed such as energy in frontier orbitals and the first molecular orbitals to work in the UV-Vis absorption spectroscopy. Electronic structure methodologies were used within the frame of the density functional theory (DFT) using the theoretical methods B3LYP/6-31G(d)//B3LYP/6-31G+(d,p) for ground states and B3LYP/6-31G(d)//CAM-B3LYP/6-31G+(d,p) for excited states. Results for the main absorption peak are in agreement with experimental results with a difference between zeaxanthin and violaxanthin results of 0.1 eV, approximately. The UV-Vis absorption spectra obtained for carotenoids are in good agreement with the experimental results. The possible use in energy generation systems is discussed for these systems. Diade chlorophyllide a-zeaxanthin was formed, and calculation results predicted energy transfer for these photosynthetic systems.
- artificial photosynthesis
- diade chl a-zx
Natural photosynthesis requires the participation of chlorophyll
In this way, mimicking photosynthesis has become a subject of great interest in the scientific world, and this global research trend has given origin to a recently created term, artificial photosynthesis [1–3]. This concept refers to a chemical process that replicates the natural process of photosynthesis; it mainly studies the process to convert sunlight, water, and carbon dioxide into carbohydrates and oxygen. This process aims to emulate natural ways by using man‐made devices to convert and store solar energy using chemical fuels as feedstock . To absorb the visible light part of the solar radiation (350–700 nm), green plants use chlorophyll
Carotenoids are important in photosynthesis, and with the mimicking of this natural process, they have raised their importance due to the fundamental need for renewable energy sources such as artificial photosynthesis . There are other fields in which carotenoids are important as well, such as food or health. Fruits and vegetables are the principal sources of carotenoids and play an important role in diet due to vitamin A activity [5–6]. In addition to this, carotenoids are also important for antioxidant activity, intercellular communication, and immune system activity [6–8]. Epidemiological studies reported that the consumption of diets rich in carotenoids is associated with a lower incidence of cancer, cardiovascular diseases, age‐related macular degeneration, and cataract formation [9–10]. Deficiency of carotenoids results in clinical signs of conjunctiva and corneal aberrations, including xerophthalmia, night blindness, corneal ulceration, scarring, and resultant irreversible blindness .
Carotenoids are classified in carotenes and xanthophylls. Carotenes contain only a parent hydrocarbon chain without any functional group, such as α‐carotene, β‐carotene, and lycopene. Xanthophylls contain oxygen as the functional group, including lutein and zeaxanthin . In plant tissues, carotenoids are typically located in chromoplasts (specific organelles) inside cells. Substructures composed of lipids, proteins, and carotenoids are being synthesized during chromoplast development, and depending on their morphology, they can be classified as crystalline, globular, fibrillar, membranous, or tubular‐type chromoplasts [13–15]. Carotenoids are embedded in a complex structural organization. Carotenes and xanthophylls contain more than seven conjugate bonds that enable visible light absorption and from here, they have the capability to participate in the photosynthesis . For light energy to be transformed into chemical energy, the electronic structure is fundamental in understanding how natural photosynthesis occurs and how this process can be associated with clean energy generation through artificial photosynthesis. Due to their chemical structure, carotenoids are tetraterpenoids so they have a long chain of conjugated double bonds; for this reason, these micronutrients are highly lipophilic [13, 16–18].
There are different determination methods to find out the basic chemical structure of carotenoids. Their structure is based on eight isoprenoid units with a conjugated double‐bond system, which makes isomeric forms very common . In addition, double bonds in the carbon chain make carotenoids susceptible to reactions, such as oxidation and isomerization (cis‐trans), especially due to light, heat, acids, and oxygen . Cyclization, hydrogenation, dehydrogenation, or additions of lateral groups, among others, are some modifications that lead to an extremely complex variety of compounds with common structures .
Moreover, carotenoids can be found in nature both in their free form and also in a more stable, esterified form with fatty acids . The high variability in their chemical structure and their poor stability greatly contribute to the difficulty of carotenoid analysis. Also, there is a lack of commercially available standards and other important reasons  that make it difficult to have more analytical methods to identify and to measure carotenoids in real samples [21–23].
Another analytic alternative is related to theoretical methods. There is a wide chart of choices to model and simulate these compounds that range from macro‐, micro‐, and atomic to subatomic methodologies. In this work, we use density functional theory (DFT) to learn more about carotenoids. We use DFT to model and simulate carotenoids’ ground states as well as excited states and analyze their electronic structure. Fundamentally, carotenoids have a strong absorption of visible light in the blue and green region of the solar spectrum. Most carotenoids found in photosynthetic organisms have the characteristic colors yellow, orange, and red. The lowest excited single state in most pigment molecules represents the lowest energy, which optically allows a one‐photon transition from the ground state . This chapter provides numerical data to parameterize some of the more important properties of carotenoids. It provides a good insight about their important role in both natural and artificial photosynthesis, and since these results relate to its more basic features, it can be useful for other applications as well, such as in the food and health industries.
2. Computational methods and details
All calculations were carried out employing Gaussian 09 program suite . This chapter was developed with computational calculations employing electronic structure methods using density functional theory (DFT). Then, a vibrational frequencies’ calculation was carried out to corroborate a global minimum. These calculations, geometry optimization, and vibrational frequencies were performed in the gaseous phase, using a methanol‐like solvent. Molecular orbitals for the different carotenoids were obtained with energy calculations using the B3LYP/6‐31G(d)//B3LYP/6‐31+G(d,p) theoretical method . Excited states in the gaseous phase were carried out for all six carotenoid variants within this work and in the solvent phase for xanthophylls molecules. These later calculations allowed us to obtain molecular orbitals and absorption states. The same set of calculations used in carotenoids was applied to chlorophyll
3. Results and discussions
In this section, calculations results are displayed and analyzed. The first set of results contains ground states data; first, the geometric structures are displayed and next the energy results are displayed, including molecular orbitals, energy gap, and relevant chemical properties. In the second set of results are included excited states data with their corresponding molecular orbital diagrams and absorption spectra based on TDDFT calculations.
3.1. Carotenoid structures
Carotenoids included in this work are displayed in Figure 1. Beta‐carotene and lycopene are carotenes with the characteristic of belonging to the hydrocarbons group, which means that their structure includes only carbon and hydrogen atoms. The rest are four carotenoids that belong to the xanthophylls which characterize themselves by containing within their structure carbon and hydrogen with oxygen atoms bonded to the six‐carbon ring.
3.1.1. Carotenoids ground states
The ground states energy results allow us to obtain data related to electronic conduction capabilities of the selected molecules in their ground states. For these calculations, the interpretation scheme of the difference between the Highest Occupied Molecular Orbital (HOMO) and the Lowest Unoccupied Molecular Orbital (LUMO) was applied, where HOMO and LUMO are frontier orbitals located in the valence band and in the conduction band, respectively. Resulting values for these orbitals relate to ionization energy in the case of HOMO, which means that a lower ionization energy corresponds to a higher HOMO energy. In fact, a lower energy for LUMO is associated with a higher electronic affinity. These effects were explained in more detail in our published work  and can be studied by further reading Table 1 as shown ahead in this section.
Frontier molecular orbitals’ values are shown in Table 1, and Figure 2 displays molecular orbitals obtained for the six selected carotenoid structures in their gaseous phase that will be discussed next. The diagram is divided into two sections: the first belongs to carotenes and the second to xanthophylls. The HOMO‐LUMO energy gap is obtained by calculating the difference between frontier orbitals’ energy values. Beta‐carotene and lycopene energy gap results have a small difference. Then, it is observed that these compounds present a similar trend in their HOMO orbitals. Based on these results, xanthophylls have a higher ionization energy which derives in the capability present in one of these variants to form a diade integrated system configured by chlorophyll
From xanthophylls displayed in the molecular orbitals diagram, one can see that zeaxanthin has the narrower HOMO‐LUMO energy gap. A narrow HOMO-LUMO energy difference benefits energy transfer process.
Photosynthesis requires that plants containing chlorophyll
In photosynthesis, carotenoids play the role of the aforementioned accessory pigments. Excited states analysis explains xanthophylls’ performance as pigments that are part of the photosynthetic process.
Figure 3 displays a molecular orbital diagram for excited states corresponding to all molecules within our chapter. This diagram, as occurred for ground states, provides information discussed previously in this section.
3.1.2. Carotenoids excited states molecular orbitals
As occurs in ground states, in excited states, zeaxanthin is the molecule with the narrower energy gap from the xanthophylls‐type carotenoids which means this is the molecule that can be excited more easily. Other data important to consider in excited states analysis is the UV‐Vis absorption spectra. Absorption spectra enable us to identify the wavelength in which a pigment absorbs sunlight and thus locate the reference electromagnetic radiation working range and the visible light required to favor photosynthesis.
Now, depending on the solvent used, sunlight absorption may have a benefit. In general, the absorption extends in larger wavelength when the solvent is employed if compared to the absorption results in the gaseous phase. Figure 4 displays the diagram of excited states molecular orbitals with the use of solvents, which in this case is methanol. This excited states calculation was carried out only for xanthophylls because zeaxanthin is the accessory pigment used to form the diade with chlorophyll
If one observes the molecular orbitals diagram corresponding to the molecules in methanol as a solvent, one can see that zeaxanthin is the molecule with the narrower energy gap as occurred in the gaseous phase calculations. Another important observation is how violaxanthin keeps the same values in both calculations, in the gaseous phase and in the solvent phase.
3.1.3. Carotenoids excited states UV‐Vis spectra
Absorption spectra for carotenoids‐type beta‐carotene and lycopene for the gaseous phase are shown in Figure 5. In this figure, a high coincidence in the absorption maximum peak between beta‐carotene with 472 nm and lycopene with 476 nm is observed. According to these calculations, the maximum absorption peak for beta‐carotene is surpassed by 20 nm. Meanwhile for lycopene, a better approximation with the experimental value of 470 nm is obtained.
3.1.4. Xanthophylls excited states UV‐Vis spectra
Absorption spectra for the gaseous phase and with the solvent for xanthophylls‐type carotenoids are displayed in Figure 6. According to the figures, one can observe that the variation in absorption results in the gaseous phase with respect to the solvent phase is different only by a wavelength displacement. Zeaxanthin is the carotenoid from xanthophylls with an absorption in a longer wavelength, similar to lutein. The difference between them is the absorbance value where zeaxanthin has the bigger absorbance.
Diades structures are formed with two molecules, one from the group of the main photosynthetic pigments and the other from the accessory pigments group. For this work, we used chlorophyll
For the intermolecular energy transfer study, photosynthetic pigments’ diade formed by chlorophyll
Since electronic properties of selected photosynthetic pigments depend of nature in both cases, the ground and the excited states, we moved forward with molecular orbitals analysis for isolated molecules and diade systems (in both ground and excited states). TDDFT was used, with Tamm‐Dancoff approximation (TDA) and the CAMB3LYP/6‐31+G(d,p) theoretical method.
After HOMO/LUMO energy analysis, UV‐Vis absorption spectra were obtained for isolated molecules and their corresponding diade systems. The same theoretical level was used with same functional set.
3.2.1. Diade molecular orbitals (HOMO/LUMO energies)
Results for molecular orbitals and HOMO‐LUMO energies corresponding to chlorophyll
Figure 7 shows an energy gap for chlorophyllide
For the diade system, it was found that the HOMO molecular orbital is provided by the corresponding carotenoid zeaxanthin, while the LUMO orbital corresponds to chlorophyllide
3.2.2. Absorption UV‐vis spectra for chlorophyllide
aand chlorophyll a
From excited states energy calculations, UV‐vis absorption spectra were determined for each of the two selected molecules including integrated systems in the diade. In Figure 9 are displayed UV‐vis absorption spectra for experimental and theoretical (corresponding to experimentation similar to calculations within this work) frameworks for chlorophyll
In Figure 9 are shown experimental values for the more characteristic chlorophyll
As can be seen in Figure 9, chlorophyllide
Regarding theoretical UV‐Vis absorption spectra (theoretical), calculations were developed for the two molecules integrated in the diades, and their diagram was based on the excited states’ results. In Figure 10, chlorophyllide
Theoretical calculations allowed us to predict UV‐Vis absorption spectra for carotenoid structures selected for this work, achieving a good accuracy with experimental results.
Within the selected carotenoids that were analyzed, zeaxanthin is the pigment with better electronic properties to form an integrated system, chlorophyll
Excited states molecular orbitals analysis for the diade system formed by chlorophyllide
Analysis of pigments related to the natural photosynthetic process allowed us to set a basis on how these pigments can be used in potential artificial photosynthetic applications and to develop new materials for alternative energy applications.
Electronic structure data are fundamental information for any material and may help to develop systems with carotenoids and accessory pigments in other fields such as the health or food industries.
The authors thank the National Council of Science and Technology (CONACYT, for its acronym in Spanish) for the financial support in the development of this scientific research to the basic science project, No. 158307. FTR and JMB thank CONACYT for a scholarship to support their doctoral studies.