Ca (m/z; 42.959) ion dissolution (Kim Y.K., et al. Autogenous teeth used for bone grafting: a comparison to traditional grafting materials. Oral Surg. Oral Med. Oral Pathol. Oral Radiol., 2013, in press)
1. Introduction
Autogenous bone, allogenic bone, xenogenic bone, and alloplastic materials are bone graft materials that are presently used in dental clinics. According to bone healing mechanism, they can be categorized into materials that induce osteogenesis, osteoinduction, and osteoconduction. Among the many different types of bone graft materials, autogenous bone is the most ideal since it is capable of osteogenesis, osteoinduction, and osteoconduction. Its advantage is the rapid healing time without immune rejection. As its biggest shortcomings, however, the harvest amount is limited, bone resorption after graft is unavoidable, and second defect is generated in the donor area. Therefore, to overcome such shortcomings, allogenic bone and synthetic bone were developed and used in clinics, and efforts have been made to develop more ideal bone substitution materials [1]. Lately, researchers and clinicians have become interested in the use of human dentin from extracted teeth in the context of autogenous bone grafts [2,3]. Dentin has inorganic and organic components that are very similar to those of human bone. In dentin, the inorganic content is 70 ~ 75%, whereas the organic content is about 20%. In alveolar bone, the inorganic content is 65%, and the organic content is 25%. At least 90% of organic content of dentin is type I collagen, which plays an important role in bone formation and mineralization. Dentin also contains bone morphogenetic proteins (BMP), which promote the differentiation of mesenchymal stem cells into chondrocytes and consequently enhance bone formation. In addition, both alveolar bone and teeth are derived from neural crest cells [4-6]. Thus, studies have been done to use fresh tooth in the form of demineralized dentin matrix (DDM) as a biocompatible autogenous bone graft material in alveolar bone repair. Butler, et al [7] and Conover and Urist, et al [8] successfully extracted bone BMP from rabbit DDM, and Bessho, et al [9] secured new bone formation
2. Osteoinduction of AutoBT
Many researchers have examined tooth dentin as a potential carrier for human proteins and as grafting material because its biological composition is very similar to that of alveolar bone [9, 24-28]. Both tooth and alveolar bone are derived from neural crest cells and are made up of the same Type I collagen. Furthermore, dentin contains BMPs, which induce bone formation and noncollagenous proteins such as osteocalcin, osteonectin, and dentin phosphoprotein [29, 30]. Since its investigation by Urist in 1965, BMP has been widely studied and used in clinical applications [31]. As a result, Yeoman and Urist, et al (1967) and Bang and Urist, et al (1967) showed the osteoinductivity of rabbit DDM by BMP [32, 33]. Bessho, et al extracted BMP from bone matrix, dentin matrix, and wound tissue after extracting teeth from rabbits. Each BMP was confirmed to have induced the formation of new bone when xenogenic implantation was performed [9]. Bessho, et al extracted human dentin matrix containing 4mol/L guanidine HC1 and refined it into liquid chromatography and found out based on SDS-PAGE and IEF that purified BMP is homogenous, inducing the formation of new bone within 3 weeks of implantation in muscle pouches in Wistar rats. Dentin matrix-derived BMP is not exactly same as bone matrix-derived BMP, but they are very similar. In other words, two types of BMP exhibit the same action in the body [34]. The organic component accounts for about 20% of dentin weight and mostly consists of type I collagen. Moreover, it was proven to have BMP promoting cartilage and bone formation, and differentiating undifferentiated mesenchymal stem cells into chondrocytes and osteogenic cells [30, 35-37]. Noncollagenous proteins of dentin such as osteocalcin, osteonectin, phosphoprotein, and sialoprotein are known to be involved in bone calcification [38,39].
Patterns of matrix protein in teeth must have osteoinductive potential even though it does not perfectly match the protein in alveolar bone. Moreover, the apatite in teeth has long been known to play the role of protecting proteins [40]. According to Boden, et al, LIM mineralization protein 1 (LMP-1) is an essential positive regulator of osteoblast differentiation and maturation and bone formation [41]. Wang, et al found that LIM-1 was expressed primarily in predentin, odontoblasts, and endothelial cells of the blood vessels of teeth [42].
Many researchers have observed that alveolar bone formation occurs around bone graft materials as a result of experiments on animals [43-47]. Chung registered the patent for the technology of extracting proteins from teeth in 2002 and 2004; this carries an important, serving as evidence that teeth contain bone morphogenic protein [48,49]. Ike and Urist suggested that root dentin prepared from extracted teeth may be recycled for use as carrier of rhBMP-2 because it induces new bone formation in the periodontium [10]. Murata, et al reported that demineralized dentin matrix (DDM) does not inhibit BMP-2 activity but shows better release profile of BMP-2. Human recycled DDM is an unique, absorbable matrix with osteoinductivity, and DDM should be an effective graft material as a carrier of BMP-2 and a scaffold for bone-forming cells for bone engineering [2].
Lee [50] performed quantitative analysis of proliferation and differentiation of the MG-63 cell line on the bone grafting material using human tooth. This study demonstrated that the cellular adhesion and proliferation activity of the MG-63 cell on partially demineralized dentin matrix (PDDM) were comparable to control with enhanced osteogenic differentiation (Figure 1). Kim & Choi [51] reported a case on tooth autotransplantation with autogenous tooth bone graft. The extracted right mandibular third molar of a 37-year-old man was transplanted into the first molar area, and a bone graft procedure using autogenous tooth-bone graft material was performed for the space between the root and the alveolar socket. Reattachment was achieved (Figure 2). Therefore, the autogenous tooth bone graft material is considered reasonable for bone inducement and healing in the autotransplantation of teeth.
Recently, we conducted a study to demonstrate the osteoinductivity of AutoBT when fabricated from bio-recycled dysfunctional teeth after patented processing. A total of 46 extracted dysfunctional teeth samples were collected from actual patients.
3. Osteoconduction of AutoBT
The analytic results showed that AutoBT consisted of low-crystalline hydroxyapatite (HA) and possibly other calcium phosphate minerals (ß-tricalcium phosphate (ß-TCP), ACP, and OCP), similar to the minerals of human bone tissues. Note, however, that the level of HA crystallization and the amount of HA differed greatly depending on the area of the tooth. The XRD pattern was much stronger in the crown portion with enamel than in the root portion (Figure 7). Likewise, the dental crown portion consisted of high-crystalline calcium phosphate minerals (mainly HA) with higher Ca/P ratio, whereas the root portion was mainly made up of low-crystalline calcium phosphates with relatively low Ca/P ratio [3, 23]. Kim, et al [52] performed the study to evaluate the surface structures and physicochemical characteristics of a novel autogenous tooth bone graft material currently in clinical use. The material’s surface structure was compared with a variety of other bone graft materials via scanning electron microscope (SEM). The crystalline structure of the autogenous tooth bone graft material from the crown (AutoBT crown) and root (AutoBT root), xenograft (BioOss), alloplastic material (MBCP), allograft (ICB), and autogenous mandibular cortical bone were compared using x-ray diffraction (XRD) analysis. The solubility of each material was measured with the Ca/P dissolution test. The result of the SEM analysis showed that the pattern associated with AutoBT was similar to that from autogenous cortical bone (Figure 8). In the XRD analysis, AutoBT root and allograft showed a low crystalline structure similar to that of autogenous cortical bone (Figure 9). In the CaP dissolution test, the amount of calcium and phosphorus dissolution in AutoBT was significant from the beginning, displaying a pattern similar to that of autogenous cortical bone (Tables 1, 2). In conclusion, autogenous tooth bone graft materials can be considered to have physicochemical characteristics similar to those of autogenous bone.
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3 d | 54.2 | 97.7 | 35.5 | 230.7 | 280.0 | 246.8 |
7 d | 48.6 | 71.7 | 33.6 | 162.7 | 255.2 | 189.2 |
14 d | 62.7 | 97.6 | 35.1 | 144.5 | 180.6 | 180.6 |
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3 d | 301.7 | 217.8 | 174.0 | 269.8 | 269.4 | 260.5 |
7 d | 311.4 | 191.2 | 151.7 | 282.8 | 230.2 | 282.8 |
14 d | 302.5 | 165.4 | 148.7 | 253.8 | 229.0 | 245.3 |
In an in vitro dissolution test, AutoBT showed excellent biodegradability, whereas apatite re-precipitation was actively visible immediately after transplantation. We conjecture that this material plays an effective role in inducing bone regrowth [52]. Priya, et al [53] reported that the extensive dissolution of calcium phosphate composites, which release calcium and phosphorus ions, induces the re-precipitation of the apatite onto the surfaces. According to them, the combination of dissolution and re-precipitation was the mechanism behind apatite formation. Apatite layer formation was expected to encourage the osseointegration of bioceramic composites.
Both the organic and inorganic compositions differ between the crown and root of autogenous tooth bone graft materials. Thus, when the material is grafted, crown and root show different healing mechanisms. Apatites present in bone tissues form a ceramic/high-molecular weight nanocomplex pattern [54]. In particular, apatites present in human bone tissues have low crystallinity and crystal size that are several tens of nanometers. On the other hand, hydroxyapatites prepared via the sintering process at high temperatures have high crystallinity. Grain growth occurs during the sintering process, resulting in sizes that are at least ten times larger than those apatites present in bone tissues [55]. The biodegradation of large particles with high crystallinity is almost impossible. Their osteoconduction capacity is very low, and osteoclasts cannot degrade them. Low-crystalline carbonic apatites show the best osteoconduction effects [56,57].
Nampo, et al introduced alveolar bone repair using extracted teeth for the graft material. DSP is a dentin-specific noncollagenous protein involved in the calcification of dentin. Based on immunohistochemical staining with anti-DSP antibody, the positive reaction was localized to the dentin of the extracted tooth fragments; thus suggesting that dentin has high affinity for and marked osteoconductive effect on the jaw bone [58].
Kim, et al reported bone healing capacity of demineralized dentin matrix materials in a mini-pig cranium defect [59]. A defect was induced in the cranium of mini-pigs, and those without defect were used as control. In the experimental group, teeth extracted from the mini-pig were manufactured into autogenous tooth bone graft material and grafted to the defect. The mini-pigs were sacrificed at 4, 8, and 12 weeks to evaluate histologically the bone healing ability and observe the osteonectin gene expression pattern with RT-PCR. At 4 weeks, the inside of the bur hole showed fibrosis, and there was no sign of bone formation in the control group. On the other hand, bone formation surrounding the tooth powder granule was observed at 4 weeks in the experimental group wherein the bur hole was filled with tooth powder. There was practically no osteonectin expression in the control group, whereas active osteonectin expression was observed from 4 to 12 weeks in the experimental group. In this study, excellent osteoconductive healing of autogenous tooth bone graft material was confirmed (Figure 10, 11).
4. Clinical application of AutoBT
Kim, et al developed a novel bone grafting material using autogenous teeth (AutoBT) in 2008 and provided the basis for its clinical application. Having organic and inorganic mineral components, AutoBT is prepared from autogenous grafting material; thus eliminating the risk of immune reaction that may lead to rejection. AutoBT was used at the time of implant placement -- simultaneously with guided bone regeneration -- and excellent bone healing by osteoinduction and osteoconduction was confirmed [3]. In a total of 6 patients, guided bone regeneration was performed simultaneously at the time of implant placement, and tissue samples were then harvested at the time of the second surgery with the patient’s consent. In the histomorphometric analysis of the samples collected from 6 patients during the 3 ~ 6 months’ healing period, new bone formation was detected in 46 ~ 87% of the area of interest, and excellent bone remodeling was achieved (Table 3) (Figure 12). Clinically available AutoBT consists of powder, chips, and block (Figure 13).
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1 |
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#24 | 3 | 43:11:46 | 74 |
2 |
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#17 | 4 | 85:14:1 | 87 |
3 |
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#17 | 6 | 56:39:5 | 46 |
4 |
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#24 | 5 | 84:12:4 | 73 |
5 |
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#36 | 3 | 51:1:48 | 52 |
6 |
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#25-27 | 6 | 65:0:35 | 68 |
Lee and Kim [60]performed a retrospective study to evaluate the clinical efficacy of AutoBT. This study included 37 patients (54 implants) into which AutoBT was grafted between Oct. 2008 and Dec. 2009. The mean follow-up period was 31 months. Postoperative complications and marginal bone status around the implants were evaluated using medical records and dental radiography. Wound dehiscence and hematoma developed in 7 patients (8 implants). Osseointegration failure in 2 patients (4 implants) was recorded. These complications were well managed through conservative treatment and re-implantation. Mean peri-implant marginal bone loss 1 year after implant placement was 0.33±0.63mm. Autogenous tooth bone graft was confirmed to be a safe procedure, showing excellent bone healing through a 2-year retrospective study (Tables 4, 5, 6).
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GBR | 29 (53.7%) |
Sinus graft (lateral approach) | 14 (25.9%) |
Sinus lifting (crestal approach) | 7 (13.0%) |
Ridge augmentation | 4 (7.4%) |
Total | 54 (100%) |
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Powder | 32 (86.5%) |
Block | 2 (5.4%) |
Powder + Block | 3 (8.1%) |
Total | 37 (100%) |
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Wound dehiscence | 7 |
Hematoma | 1 |
Osseointegration failure | 4 |
Total | 12 |
5. Sinus bone graft
If there is any material whose resorption speed is not too high and whose bone healing process approximates that of autogenous bone graft, it may be useful in maxillary sinus bone grafting. Likewise, more excellent clinical achievement may be expected when these materials are used in mixture with other bone substitutes with slow resorption properties [61,62,63]. With evidence presented in the foregoing paragraphs, AutoBT® developed by the author, et al was proven to exhibit bone healing ability through osteoinduction and osteoconduction, demonstrating a histological healing process similar to that of free bone grafting being resorbed over 3~6 months [3]. Accordingly, AutoBT® is regarded as a possible substitute when autogenous bone is needed for sinus bone graft, and it may wield a useful effect on increasing the volume of bone graft materials and minimizing repneumatization (Figure 14).
A retrospective study on sinus bone graft was performed. One hundred implants in 51 patients were selected, with the patients receiving maxillary sinus augmentation and implant placement using autogenous tooth graft materials at Chosun University Dental Hospital and Seoul National University Bundang Hospital (SNUBH) between July 2009 and November 2010. In cases of using autogenous tooth bone graft alone or together with other graft material, the implant survival rate was 96.15%. Based on the histomorphologic examination, autogenous tooth bone graft materials showed gradual resorption and new bone formation through osteoconduction and osteoinduction. The results suggest that autogenous tooth bone graft materials are appropriate for use in maxillary sinus augmentation [64].
Lee, et al [65] conducted a study to evaluate histomorphometrically and compare the efficiency of various bone graft materials and autogenous tooth bone graft material used in the sinus bone graft procedure. The subjects were 24 patients who had been treated with sinus bone graft using the lateral approach from October 2007 to September 2009 at SNUBH. The average age was 52.51±11.86 years. All cases were taken after 4 months of procedure and divided into 3 groups according to bone graft material: Group 1 for autogenous tooth bone graft material (AutoBT), Group 2 for OrthoblastII (Integra Lifescience Corp., Irvine, US)+Biocera (Osscotec, Cheonan, Korea), and Group 3 for DBX (Synthes, West Chester, PA, USA), BioOss (Geistlich Pharm AG, Wolhusen, Switzerland). A total of 37 implant placement areas was included and evaluated (7 in group 1, 10 in group 2, 20 in group 3). The evaluation of new bone formation, ratio of woven bone to lamellar bone, and ratio of new bone to graft material was performed on each tissue section. The Kruskal-Wallis test was used for statistical analysis (SPSS Ver. 12.0, USA). New bone formation was 52.5±10.7 % in group 1, 52.0±23.4% in group 2, and 51.0±18.3% in group 3 (Table 7) (Figure 15-18). There were no statistically significant differences between groups, however. The ratio of woven bone to lamella bone was 82.8±15.3% in group 1, 36.7 ±59.3% in group 2, and 31.0±51.2% in group 3. The ratio of new bone to graft material was 81.3±10.4% in group 1, 72.5±28.8% in group 2, and 80.3±24.0% in group 3. After a 4-month healing period, all groups showed favorable new bone formation and around the graft material and implant. Within the limitation of our study, autogenous tooth bone graft material may be used as a novel bone graft material for sinus bone graft. Kim, et al and Lee, et al performed sinus bone graft and guided bone regeneration using autogenous tooth bone from humans and took the tissue specimen 2 months and 4 months later for histomorphometric analysis. They found favorable new bone formation as a result and suggested that autogenous tooth bone graft materials could be used in various bone grafts [65,66].
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I | 52.5±10.7% |
II | 52.0±23.4% |
III | 51.0±18.3% |
6. Guided bone regeneration
Bone dehiscence or bone fenestration often develops after dental implant placement, and guided bone regeneration using bone graft materials has become a popular method. The most ideal material for guided bone regeneration is autogenous bone, but autogenous bone graft has limited sources and high risk of complications at the donor site and causes high resorption after bone graft. Therefore, alternative bone materials have been developed and used clinically, such as allogenic bone, xenogenic bone, and synthetic bone. Note, however, that they are often mixed with autogenous bone to maximize their advantages.
Autogenous teeth bone graft materials have very good osteoinductive and osteoconductive properties due to the organic and inorganic contents of the teeth, such as collagen, bone growth factors, and various forms of calcium phosphate. In our study, we achieved 46~74% new bone formation in 3~6 months compared with the results of Babbush [3,67]. Considering the histological healing of the sites where autogenous teeth bone graft materials were applied, bone graft materials were replaced with new bone following resorption, and new bone directly fused with the remaining autogenous teeth bone graft materials. A healing process associated with excellent osteoinduction and osteoconduction was observed in every sample, including abundant lamella bone; thus indicating that rapid bone reconduction was occurring [50,51,59,65,66]. Kim, et al [68] installed implants combined with guided bone regeneration using autogenous tooth bone graft material in 6 patients. In the 6 months’ histological examination after operation, excellent osteoconductive bone healing was noted. A clinically favorable outcome was obtained (Figure 19~21).
7. Ridge augmentation (Figure 22)
Autogenous bone grafting produces the best results in case a large volume of bone increase is required, as in the reconstruction of a site with lots of bone defects or ridge augmentation. The autograft may be taken from the endochondral bone such as ilium, rib, tibia, etc., and from the intramembranous bone such as calvaria, facial bone, etc. Alveolar ridge augmentation is a method of augmenting the height or width of the alveolar ridge by implementing bone grafting on the upper part or lateral part of the ridge in particulate or block type in case bone volume is insufficient vertically or horizontally; vertical and horizontal augmentation may be done simultaneously, but it may also be carried out individually. Since it is a kind of onlay graft, bone resorption occurs considerably after grafting, and dehiscence on the upper soft tissue easily arises [69]. Meanwhile, as for the autogenous bone graft, there may be some complications on the donor site, and doing the grafting takes time. Likewise, there are several problems such as limit to the volume of collection. Consequently, patients and clinical doctors are inclined to avoid it in many cases. As substitutes for autograft, bone graft materials such as allograft, xenograft, synthetic bone, etc., were developed, but the single use of each is not recommended in the method of augmenting bone tissue vertically or horizontally [69,70]. For the vertical or horizontal ridge augmentation, AutoBT may be a substitute method for autogenous bone graft and may be very useful in clinical practices when used in mixture with other graft materials in case of insufficient volume. Kim, et al. [71,72] reported the successful case of alveolar ridge augmentation using various autogenous tooth bone graft materials.
8. Extraction socket preservation or reconstruction (Figure 23)
The resorption of the residual alveolar bone in the vicinity of extraction sockets reportedly occurs primarily during the initial period after tooth extraction; in cases wherein teeth are infected with periodontal diseases, it shows more severe resorption [73]. Severe resorption of the alveolar bone may cause aesthetic problems in the anterior teeth. In addition, normal, natural healing may be difficult since the soft tissues may fall down into the defective area if there is progressive periodontal disease or periapical inflammatory lesion, or in case of serious defects of the surrounding bone wall after tooth extraction. Therefore, the preservation or reconstruction of the extraction sockets should be considered positively in case of serious defects after tooth extraction [74]. Ridge preservation methods using various bone graft materials were introduced and reported to be effective in preventing vertical and horizontal ridge resorption [75-77]. Kim, et al [78] reported an actual case of extraction socket preservation and reconstruction using autogenous tooth bone powder and block. They reported good healing of extraction socket after 3~3.5 months, and they could successfully perform the placement of implants.
9. Conclusion
It is obvious that autogenous tooth bone graft materials(AutoBT) are safer than allogeneic and xenogeneic bon egraft materials; the fact that they are compared with the healing performance of free autogenous bone graft in histological view is clear evidence. AutoBT can be used safely in a variety of bone reconstructive procedures such as sinus bone graft, GBR, ridge augmentation and extraction socket graft.
References
- 1.
Bone-grafting materials in implant dentistry. Implant DentMisch C. E Dietsh F 1993 2 3 158 167 - 2.
Human Dentin as Novel Biomaterial for Bone Regeneration. In: Rosario Pignatello R. (ed.) Biomaterials- Physics and Chemistry, InTech;Murata M Akazawa T Mitsugi M Um I. W Kim K. W Kim Y. K 2011 127 140 - 3.
Development of a novel bone grafting material using autogenous teeth. Oral Surg Oral Med Oral Pathol Oral Radiol Endod.Kim Y. K Kim S. G Byeon J. H Lee H. J Um I. U Lim S. C Kim S. Y 2010 109 4 496 503 - 4.
Ten Cate’s Oral Histology, 7thedi. Elsevier Inc.Nanci A 2008 202 211 - 5.
Oral Biochemistry. Daehan Narae Pub Co. Seoul.Min B. M 2007 22 26 - 6.
Orban’s Oral histology and embryology. 9th edition. Mosby Co. USA.Bhaskar S. N 1980 - 7.
Noncollagenous proteins of a rat dentin matrix possessing bone morphogenetic activity. J Dent ResButler W. T Mikulski A Urist M. R 1977 56 3 228 232 - 8.
Induction of heterotopic bone formation by demineralized dentin in guinea pigs: antigenicity of the dentin matrix. J Oral PatholBang G 1972 1 4 172 185 - 9.
Purification of rabbit bone morphogenetic protein derived from bone, dentin, and wound tissue after tooth extraction. J Oral Maxillofac SurgBessho K Tagawa T Murata M 1990 48 2 162 169 - 10.
Recycled dentin root matrix for a carrier of recombinant human bone morphogenetic protein. J Oral ImplantolIke M Urist M. R 1998 24 3 124 132 - 11.
An experimental study on the tissue reaction of toothash implanted in mandible body of the mature dog. J Korean Assoc Maxillofac Plast Reconstr SurgKim Y. K Yeo H. H Ryu C. H Lee H. B Byun U. R Cho J. E 1993 15 129 136 - 12.
Implantation of toothash combined with plaster of paris: Experimental study. J Korean Assoc Maxillofac Plast Reconstr SurgKim Y. K Yeo H. H Yang I. S Seo J. H Cho J. O 1994 16 122 129 - 13.
The experimental study of the implantation of toothash and plaster of Paris and guided tissue regeneration using Lyodura. J Korean Assoc Oral Maxillofac SurgKim Y. K 1996 22 297 306 - 14.
Transmitted electronic microscopic study about the tissue reaction after the implantation of toothash. J Korean Assoc Oral Maxillofac SurgKim Y. K Yeo H. H 1997 23 283 289 - 15.
An experimental study on the healing process after the implantation of various bone substitutes in the rats. J Korean Assoc Oral Maxillofac SurgKim Y. K Kim S. G Lee J. G Lee M. H Cho J. O 2001 27 15 24 - 16.
Cytotoxicity and hypersensitivity test of toothash. J Korean Maxillofac Plast Reconstr SurgKim Y. K Kim S. G Lee J. H 2001 23 391 395 - 17.
Grafting of large defects of the jaws with a particulate dentin-plaster of Paris combination. Oral Surg Oral Med Oral Pathol Oral Radiol EndodKim S. G Yeo H. H Kim Y. K 1999 88 1 22 25 - 18.
Combined implantation of particulate dentine, plaster of Paris, and a bone xenograft (Bio-Oss) for bone regeneration in rats. J Craniomaxillofac SurgKim S. G Kim H. K Lim S. C 2001 29 5 282 288 - 19.
Use of particulate dentin-plaster of Paris combination with/without platelet-rich plasma in the treatment of bone defects around implants. Int J Oral Maxillofac ImplantsKim S. G Chung C. H Kim Y. K Park J. C Lim S. C 2002 17 1 86 94 - 20.
Effects on bone formation in ovariectomized rats after implantation of toothash and plaster of Paris mixture. J Oral Maxillofac SurgKim S. Y Kim S. G Lim S. C Bae C. S 2004 62 7 852 857 - 21.
Osteogenic activity of the mixture of chitosan and particulate dentin. J Biomed Mater Res APark S. S Kim S. G Lim S. C Ong J. L 2008 87 3 618 623 - 22.
Effect of Tisseel on bone healing with particulate dentin and plaster of Paris mixture. Oral Surg Oral Med Oral Pathol Oral Radiol EndodKim W. B Kim S. G Lim S. C Kim Y. K Park S. N 2010 e34 e40. - 23.
Analysis of the inorganic component of autogenous tooth bone graft material. J Nanosci Nanotechnol.Kim Y. K Kim S. G Oh J. S Jin S. C Son J. S Kim S. Y Lim S. Y 2011 11 8 7442 7445 - 24.
Bone induction in excavation chambers in matrix of decalcified dentin. Arch SurgBang G Urist M. R 1967 94 6 781 789 - 25.
Noncollagenous proteins of a rat dentin matrix processing bone morphogenetic activity. J Dent ResButler W. T Mikulski A Urist M. R 1977 56 3 228 232 - 26.
Transmembrane bone morphogenesis by implanted of dentin matrix. J Dent ResConover M. A Urist M. R 1979 - 27.
Dentin matrix morphogenetic protein. The Chemistry and Biology of Mineralized Connective Tissues. Northwestern University. New York;Elsevier-North Holland Inc.;Conover M. A Urist M. R 1981 597 606 - 28.
Recycled dentin toot matrix for a carrier of recombinant human bone morphogenetic protein. J Oral ImplantolIke M Urist M. R 1998 24 3 124 132 - 29.
Gene expression of growth and differentiation factors-5,-6, and-7 in developing bovine tooth at the root forming stage. Biochem Biophys Res CommunMorotome Y Goseki-sone M Ishikawa I Oida S 1988 244 1 85 90 - 30.
Bone morphogenetic protein. J Dent ResUrist M. R Strates B. S 1971 50 1393 406 - 31.
Bone: formation by autoinduction. ScienceUrist M. R 1965 150 3698 893 899 - 32.
Bone induction by decalcified dentin implanted into oral osseous and muscle tissues. Arch Oral BiolYeomans D. J Urist M. R 1967 12 8 999 1008 - 33.
Bone induction in excavation chambers in matrix of decalcified dentin. Arch SurgBang G Urist M. R 1967 94 6 781 789 - 34.
Human dentin-matrix-derived bone morphogenetic protein. J Dent ResBessho K Tanaka N Matsumoto J Tagawa T Murata M 1991 70 171 175 - 35.
Use of wounds in the parietal bone of the rat for evaluating bone marrow for grafting into periodontal defects. J Periodontal ResTurnbull R. S Freeman E 1974 9 1 39 43 - 36.
Induction of cartilage and bone by dentin demineralized in citric acid. J Periodontal ResInoue T Deporter D. A Melcher A. H 1986 21 3 243 255 - 37.
A bovine tooth derived bone morphogenetic protein. J Dent ResKawai T Urist M. R 1989 68 6 1069 1074 - 38.
CA, MacDougall Ml. Genomic organization, chromosomal mapping, and promoter analysis of the mouse dentin sialophosphoprotein (Dspp) gene, which codes for both dentin sialoprotein and dentin phosphoprotein. J Biol ChemFeng J. Q Luan X Wallace J Jing D Ohshima T Kulkarni A. B D Souza R. N Kozak 1998 273 16 9457 9464 - 39.
Six decades of dentinogenesis research. Historical and prospective views on phosphophoryn and dentin sialprotein. Eur J Oral SciRitchie H. H Ritchie D. G Wang L. H 1998 Suppl1 211 220 - 40.
Intact growth factors are conserved in the extracellular matrix of ancient human bone and teeth: a storehouse for the study of human evolution in health and disease. Biol ChemSchmidt-schultz T. H Schultz M 2005 386 8 767 776 - 41.
LMP-1, aLIM-domain protein, mediates BMP-6 effects on bone formation. EndocrinologyBoden S. D Liu Y Hair G. A Helms J. A Hu D Racine M Nanes M. S Titus L 1998 139 12 125 134 - 42.
Immunohistochemical localization of LIM mineralization protein 1 in pulp-dentin complex of human teeth with normal and pathologic conditions. J EndodWang X Zhang Q Chen Z Zhang L 2008 34 2 143 147 - 43.
Continued development of 5-day old tooth-germs transplanted to syngeneic hamster (Mesocricetus Auratus) cheek pouch. Arch Oral BiolAl-talabani N. G Smith C. J 1978 23 12 1069 1076 - 44.
An histological study of the effects of extra-corporeal time on murine dental isografts. Arch Oral BiolSteidler N. E Reade P. C 1979 24 2 165 169 - 45.
Changes in periodontal fibre organization in mature bone/tooth isografts in mice. J Oral PatholBarrett A. P Reade P. C 1981 10 276 283 - 46.
The relationship between degree of development of tooth isografts and the subsequent formation of bone and periodontal ligament. J Periodontal ResBarrett A. P Reade P. C 1981 16 456 465 - 47.
A histological investigation of isografts of immature mouse molars to an intrabony and extrabony site. Arch Oral BiolBarrett A. P Reade P. C 1982 27 59 63 - 48.
Method for extracting tooth protein from extracted tooth. Korea Intellectual Property Rights Information Service. Patent. ApplicationChung P. H 10-2002 2002 - 49.
Tooth protein extracted from extracted tooth and method for using the same. Korea Intellectual Property Rights Information Service. Patent. ApplicationChung P. H 10-2004 2004 - 50.
Quantitative analysis of proliferation and differentiation of MG-63 cell line on the bone grafting material using human tooth. PhD thesis. School of Dentistry, Seoul National University;Lee H. J 2011 - 51.
Tooth autotransplantation with autogenous tooth-bone graft: A case report. J Korean Dent SciKim Y. K Choi Y. H 2011 4 2 79 84 - 52.
Autogenous teeth used for bone grafting: A comparison to traditional grafting materials. Oral Surg Oral Med Oral Pathol Oral Radiol.Kim Y. K Kim S. G Yun P. Y et al 2013 in press. - 53.
In vitro dissolution of calcium phosphate-mullite composite in simulated body fluid. J Mater Sci Mater MedPriya A Nath S Biswas K Bikramjit B 2010 21 1817 1828 - 54.
Molecular biology of mineralized tissues with particular reference to bone. Rev Mod PhysGlimcher M. J 1959 31 359 393 - 55.
Low Crystalline hydroxyl carbonate apatite. J Korean Dental AssocLee S. H 2006 44 524 533 - 56.
The biodegradation mechanism of calcium phosphate biomaterials in bone. J Biomed Mat Res Appl BiomaterLu J Descamps M Dejou J Koubi G Hardouin P Lemaitre J Proust J. P 2002 63 4 408 412 - 57.
Hanker mayer CR, Constantz BR, Ross J. Measurements of the solubilities and dissolution rates of several hydroxyapatites. BiomaterialsFulmer M. T Ison I. C 2002 23 3 751 755 - 58.
A new method for alveolar bone repair using extracted teeth for the graft material. J PeriodontolNampo T Watahiki J Enomoto A Taguchi T Ono M Nakano H Tamamoto G Irie T Tachikawa T Maki K 2010 81 9 1264 1272 - 59.
Bone healing capacity of demineralized dentin matrix materials in a mini-pig cranium defect. J Korean Dent SciKim J. Y Kim K. W Um I. W Kim Y. K Lee J. K 2012 5 1 21 8 - 60.
Retrospective cohort study of autogenous tooth bone graft. Oral Biol ResLee J. Y Kim Y. K 2012 36 1 39 43 - 61.
A clinical long-term radiographic evaluation of graft height changes after maxillary sinus floor augmentation with a 2:1 autogenous bone/xenograft mixture and simultaneous placement of dental implants. Clin Oral Impl Res.Hatano N Shimizu Y Ooya K 2004 15 3 339 345 - 62.
Long-term results with different bone substitutes used for sinus floor elevation. J Craniofacial SurgVelich N Nemeth Z Toth C Szabo G 2004 15 1 38 41 - 63.
Current concepts in augmentation grafting of the maxillary sinus for placement of dental implants. Dent Implantol Update.Garg A. K 2001 12 3 17 22 - 64.
Clinical Study of Graft Materials Using Autogenous Teeth in Maxillary Sinus ugmentation. Implant DentJeong K. I Kim S. G Kim Y. K Oh J. S Jeong M. A Park J. J 2011 20 6 471 475 - 65.
Histomorphometric study of sinus bone graft using various graft material. J Dental Rehabilitation and Applied ScienceLee J. Y Kim Y. K Kim S. G Lim S. C 2011 27 141 147 - 66.
Histomorphometric analysis of bone graft using autogenous tooth bone graft. ImplantologyKim S. G Kim Y. K Lim S. C Kim K. W Um I. W 2011 15 134 141 - 67.
Histologic evaluation of human biopsies after dental augmentation with a demineralized bone matrix putty. Implant DentBabbush C 2003 12 325 332 - 68.
Kim SGm Um IW. Guided bone regeneration using autogenous teeth: case reports. J Korean Assoc Oral Maxillofac SurgKim Y. K Lee H. J Kim K. W 2011 37 142 147 - 69.
Lee BG: Bone Graft and Implant. Clinical application of a variety of bone graft,Kim Y. K Kim S. G 2-2 Seoul: Narae Pub Co;2007 86 134 - 70.
A variety of grafting biomaterial used in dental surgery, Seoul: Narae Pub Co.;Kim M. J Kim Y. K Kim S. G 2004 19 24 - 71.
Horizontal Ridge Augmentation using Ridge expansion and Autogenous Tooth Bone Graft: A Case Report. J Dent Rehabil Appl Sci,Kim Y. K Yi Y. J 2011 2011 1 109 115 - 72.
Vertical and horizontal ridge augmentation using autogenous tooth bone graft materials: A case report. J Korean Assoc Maxillofac Plast Reconstr SurgKim Y. K Um I. W 2011 33 2 166 170 - 73.
Clinical and histological observation of sites implanted with intraoral autologous bone grafts or allografts. 15 human case reports. J PeriodontolBecker W Urist M Becker B. E et al 1996 67 1025 1033 - 74.
Autogenous tooth bone graft. Seoul: Charmyun:Kim Y. K Um I. W 2011 192 211 - 75.
Ridge preservation of the molar extraction socket using collagen sponge and xenogeneic bone grafts. Implant DentKim Y. K Yun P. Y Lee H. J Ahn J. Y Kim S. G 2011 20 4 267 272 - 76.
The Bio-Col technique. In: Bowyers LC, ed. Soft Tissue and Esthetic Considerations in Implant Therapy. Chicago, IL: Quintessence;Sclar A 2003 2003 163 187 - 77.
Ridge preservation with freeze-dried bone allograft and a collagen membrane compared to extraction alone for implant site development: A clinical and histologic study in humans. J Periodontol.Iasella J. M Greenwell H Miller R. L Hill M Drisko C Bohra A. A Scheetz J. P 2003 74 7 990 999 - 78.
Extraction socket preservation and reconstruction using autogenous tooth bone graft. J Korean Assoc Maxillofac Plast Reconstr SurgKim Y. K Kim S. G Kim K. W Um I. W 2011 33 264 269