Open access peer-reviewed Edited Volume

Applications of Immunocytochemistry

Edited by Hesam Dehghani

Ferdowsi University of Mashhad, Iran

Immunocytochemistry is classically defined as a procedure to detect antigens in cellular contexts using antibodies. However, over the years many aspects of this procedure have evolved within a plethora of experimental setups. There are different ways to prepare a given specimen, different kinds of antibodies to apply, different techniques for imaging, and different methods of analyzing the data. In this book, various ways of performing each individual step of immunocytochemistry in different cellular contexts are exemplified and discussed. Applications of Immunocytochemistry offers technical and background information on different steps of immunocytochemistry and presents the application of this technique and its adaptations in cell lines, neural tissue, pancreatic tissue, sputum cells, sperm cells, preimplantation embryo, arabidopsis, fish gonads, and Leishmania.

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Applications of ImmunocytochemistryEdited by Hesam Dehghani

Published: March 9th 2012

DOI: 10.5772/1896

ISBN: 978-953-51-0229-8

eBook (PDF) ISBN: 978-953-51-5235-4

Copyright year: 2012

Books open for chapter submissions

46156 Total Chapter Downloads

15 Crossref Citations

30 Web of Science Citations

32 Dimensions Citations


Open access peer-reviewed

1. Immunostaining of Voltage-Gated Ion Channels in Cell Lines and Neurons – Key Concepts and Potential Pitfalls

By Elke Bocksteins, Andrew J. Shepherd, Durga P. Mohapatra and Dirk J. Snyders


Open access peer-reviewed

2. Optimizing Multiple Immunostaining of Neural Tissue

By Araceli Diez-Fraile, Nico Van Hecke, Christopher J. Guérin and Katharina D’Herde


Open access peer-reviewed

3. Immunohistochemical Correlation of Novel Biomarkers with Neurodegeneration in Rat Models of Brain Injury

By Shyam Gajavelli, Amade Bregy, Markus Spurlock, Daniel Diaz, Stephen Burks, Christine Bomberger, Carlos J. Bidot, Shoji Yokobori, Julio Diaz, Jose Sanchez-Chavez and Ross Bullock


Open access peer-reviewed

4. Immunoelectron Microscopy: A Reliable Tool for the Analysis of Cellular Processes

By Ana L. De Paul, Jorge H. Mukdsi, Juan P. Petiti, Silvina Gutiérrez, Amado A. Quintar, Cristina A. Maldonado and Alicia I. Torres


Open access peer-reviewed

5. Immunocytochemistry of Cytoskeleton Proteins

By Arzu Karabay, Şirin Korulu and Ayşegül Yıldız Ünal


Open access peer-reviewed

6. Immunocytochemical Approaches to the Identification of Membrane Topology of the Na+/Cl--Dependent Neurotransmitter Transporters

By Chiharu Sogawa, Norio Sogawa and Shigeo Kitayama


Open access peer-reviewed

7. Immunocytochemical Tools Reveal a New Research Field Between the Boundaries of Immunology and Reproductive Biology in Teleosts

By Alfonsa García-Ayala and Elena Chaves-Pozo


Open access peer-reviewed

8. Immunocytochemistry in Early Mammalian Embryos

By Hesam Dehghani


Open access peer-reviewed

9. Spermiomics: A New Term Describing the Global Survey of the Overall Sperm Function by the Combined Utilization of Immunocytochemistry, Metabolomics, Proteomics and Other Classical Analytical Techniques

By Joan E. Rodríguez-Gil


Open access peer-reviewed

10. The Plasticity of Pancreatic Stellate Cells Could Be Involved in the Control of the Mechanisms that Govern the Neogenesis Process in the Pancreas Gland

By Eugenia Mato, Maria Lucas, Silvia Barceló and Anna Novials


Open access peer-reviewed

11. Application of Immunocytochemistry to Sputum Cells to Investigate Molecular Mechanisms of Airway Inflammation

By Kittipong Maneechotesuwan and Adisak Wongkajornsilp


Open access peer-reviewed

12. The Schwann Cell-Axon Link in Normal Condition or Neuro-Degenerative Diseases: An Immunocytochemical Approach

By Alejandra Kun, Gonzalo Rosso, Lucía Canclini, Mariana Bresque, Carlos Romeo, Karina Cal, Aldo Calliari, Alicia Hanuz, José Roberto Sotelo-Silveira and José Roberto Sotelo


Open access peer-reviewed

13. Immunocytochemistry of Proteases in the Study of Leishmania Physiology and Host-Parasite Interaction

By Raquel Elisa da Silva-López


Open access peer-reviewed

14. Immuno-Glyco-Imaging in Plant Cells: Localization of Cell Wall Carbohydrate Epitopes and Their Biosynthesizing Enzymes

By Marie-Laure Follet-Gueye, Mollet Jean-Claude, Vicré-Gibouin Maïté, Bernard Sophie, Chevalier Laurence, Plancot Barbara, Dardelle Flavien, Ramdani Yasmina, Coimbra Silvia and Driouich Azeddine


Edited Volume and chapters are indexed in

  • Worldcat
  • OpenAIRE
  • Google Scholar
  • AZ ebsco
  • Base
  • CNKI

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