Open access peer-reviewed Edited Volume

Antibody Engineering

Antibody Engineering comprises in vitro selection and modification of human antibodies including humanization of mouse antibodies for therapy, diagnosis, and research. This book comprises an overview about the generation of antibody diversity and essential techniques in antibody engineering: construction of immune, naive and synthetic libraries, all available in vitro display methods, humanization by chain shuffling, affinity maturation techniques, de novo synthesis of antibody genes, colony assays for library screening, construction of scFvs from hybridomas, and purification of monoclonal antibodies by exclusion chromatography. In addition, other topics that are discussed in this book are application and mechanism of single domain antibodies, structural diversity of antibodies, immune-mediated skin reactions induced by TNF-alpha recombinant antibodies, and bioinformatic approaches to select pathogen-derived peptide sequences for antibody targets.

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Antibody EngineeringEdited by Thomas Böldicke

Published: February 21st 2018

DOI: 10.5772/65238

ISBN: 978-953-51-3826-6

Print ISBN: 978-953-51-3825-9

eBook (PDF) ISBN: 978-953-51-3975-1

Copyright year: 2018

Books open for chapter submissions

16803 Total Chapter Downloads

9 Crossref Citations

7 Web of Science Citations

18 Dimensions Citations


Open access peer-reviewed

1. Generation of Antibody Diversity

By Oliver Backhaus


Open access peer-reviewed

2. High Affinity Maturated Human Antibodies from Naïve and Synthetic Antibody Repertoires

By Chia Chiu Lim, Yee Siew Choong and Theam Soon Lim


Open access peer-reviewed

3. Display Technologies for the Selection of Monoclonal Antibodies for Clinical Use

By Lilian Rumi Tsuruta, Mariana Lopes dos and Ana Maria Moro


Open access peer-reviewed

4. Detailed Protocols for the Selection of Antiviral Human Antibodies from Combinatorial Immune Phage Display Libraries

By Philipp Diebolder and Adalbert Krawczyk


Open access peer-reviewed

5. Colony Assay for Antibody Library Screening: Outlook and Comparison to Display Screening

By Mieko Kato and Yoshiro Hanyu


Open access peer-reviewed

6. Construction and Characteristics of a Recombinant Single- Chain Antibody Fragment against Bacterial Type III Secretion

By Teiji Sawa, Atsushi Kainuma, Kiyoshi Moriyama and Yoshifumi Naito


Open access peer-reviewed

7. Separation of Monoclonal Antibodies by Analytical Size Exclusion Chromatography

By Atis Chakrabarti


Open access peer-reviewed

8. The Development of Single Domain Antibodies for Diagnostic and Therapeutic Applications

By Chiuan Herng Leow, Qin Cheng, Katja Fischer and James McCarthy


Open access peer-reviewed

9. Use, Applications and Mechanisms of Intracellular Actions of Camelid VHHs

By Anneleen Steels, Laurence Bertier and Jan Gettemans


Open access peer-reviewed

10. Structural Diversity Problems and the Solving Method for Antibody Light Chains

By Emi Hifumi, Hiroaki Taguchi, Ryuichi Kato, Mitsue Arakawa, Yoshiki Katayama and Taizo Uda


Open access peer-reviewed

11. Immune-Mediated Skin Reactions Induced by Recombinant Antibodies and Other TNF-Alpha Inhibitors

By Karolína Vorčáková, Péč Juraj, Péčová Tatiana and Martinásková Klára


Open access peer-reviewed

12. Bioinformatics as a Tool to Identify Infectious Disease Pathogen Peptide Sequences as Targets for Antibody Engineering

By Lavanya Suneetha, Prasanna Marsakatla, Rachel Supriya Suneetha and Sujai Suneetha


Edited Volume and chapters are indexed in

  • Worldcat
  • OpenAIRE
  • Google Scholar
  • AZ ebsco
  • Base
  • CNKI

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