Role of mushrooms in biosorption of dyes.
Contamination of soil, water, and air by hazardous substances is the major environmental problem of today’s world. Mushroom consumption has become a tradition among many people due to its richness in flavors, proteins, and some medicinal importance. But its ability to degrade/decolorize hazardous substances and dyes by secreting various enzymes or by absorption and adsorption of colors from waste substances has made them of interest for use in the field of bioremediation. Mushroom acts as a good decomposer as it degrades cellulose and lignin of plants for their growth and development. It also maintains soil health by performing the role of hyperaccumulators. This chapter focused on the mushroom-based biodegradation/decolorization of dyes and effluents released from various industries or other sources. It also emphasizes the probable mechanisms involved in mushroom-based degradation and decolorization of dyes along with their recent achievements, advancements, and future prospective.
- agro-industrial wastes
To fulfill the demand of growing number of people, rapid industrialization and modernization not only give useful products but also release hazardous elements to nature. The release of industrial effluents and the accumulation of toxic substances into the biosphere destroy the environment by interacting with various components of the natural ecosystem . The effluents released from textile industries, food processing industries, pharmaceutical industries, etc., containing various synthetic dyes, toxic heavy metals, and other wastes, directly or indirectly come in contact with water and soil and destroy water and soil properties by changing the pH, total organic carbon (TOC), biological oxygen demand (BOD), and chemical oxygen demand (COD) [1, 2]. Various types of synthetic dyes are used extensively in the field of textile industries for coloring purposes. For example in batik industries, Remazol Brilliant Blue R (RBBR) and naphthol are used as coloring agents. Remazol Brilliant Blue R is a heterocyclic compound, and its derivatives are toxic to the environment. On the other hand, naphthol is insoluble in water and is used to dye cellulosic fibers. Improper handling, carelessness, and inefficient dye waste treatments of industries are the main reasons for the contamination of soil and water . The concentration of carcinogenic heavy metals like As, Cd, Co, Cr, Cu, Fe, Hg, Mn, Ni, etc. are relatively high in untreated industrial wastes. The rapid depletion of dissolved oxygen in water due to the presence of toxic heavy metals and other industrial wastes leads to “oxygen sag” . Majority of the synthetic dyes are used in the field of textile industries, and the effluents are discharged as wastewater. The dyes or their breakdown products are hazardous they are found to be carcinogenic .
Remediation refers to the complete or partial removal of contaminants from the polluted sites to provide a sustainable environment. Various physical and chemical remediation technologies are developed to eliminate the pollutant from the soil and improve soil health. Higher costs, limited applications with limited opportunities, and the inability to enhance intrinsic soil health make them almost abandoned . Bioremediation refers to the use of biological agents such as microbes, plants, or any other living things that help to reduce contamination to a nontoxic level or untraceable level . Paul Stamets first coined the term “mycoremediation” based on the fungal detoxification of contaminated soil. He defined the term mycoremediation as a process of sequestration of contaminated soil or water by using fungi to reduce contaminants . Mushrooms are sources of protein and their enzymatic machinery have the ability to degrade pollutants for their growth and developments. Thus, mushroom cultivation got much more attention in the field of decolorization and biodegradation research. Mushrooms are mostly basidiomycetes, a class of fungi which secretes a variety of extracellular enzymes for their growth and development . These enzymes include laccase, lignin peroxidase (LiP), versatile peroxidase (VP), manganese peroxidase (MnP), phenoloxidases, etc. . Singh reported that the lignin degradation ability of white-rot fungi is due to the presence of phenoloxidase . Due to the potential role in bioremediation of various dyes, lignin, and cellulosic compounds, the white-rot fungi became a model organism for mycoremediation . Due to the structural similarity of polycyclic aromatic hydrocarbons (PAH), polychlorinated biphenyl (PCB), various dyes, dioxins, and pesticides with lignin, cellulosic compounds, and with their substrates, mushroom-based mycoremediation processes got much more emphasized in the recent years . This chapter focused on the mushroom-based biodegradation/decolorization of dyes and effluents released from various industries or other sources. It also emphasizes the probable mechanisms involved in mushroom-based degradation and decolorization of dyes along with their recent achievements, advancements, and future prospective.
2. Role of mushroom in decolorization of dyes
Industries like textile, food processing, chemical, leather, dyestuff, dyeing, and pharmaceutical industries release a huge number of effluents containing various types of dyes. Textile industries are thought to be leading producers of dyes, and the dyes released from industries directly penetrate into the soil and water and disturb the natural ecosystem . Around 80,000 tons of dyestuff and shades are created in India. It has been assessed that 10,000 distinctive colored materials are economically accessible worldwide and the yearly generation is evaluated to be 7 × 105 metric tons . In the field of textile industries, azo dyes are mostly used as a colorant agent. The presence of one or more azo groups helps to prevent the molecules from breakdown and degradation and hence persistently accumulated into the environment. The entry of industrial effluents into the water causes a drastic reduction of dissolved oxygen; as a result great environmental damage will occur . The entry of dyes into the aquatic ecosystem disturbs light penetration into the deeper part; as a result, reduction of water quality, photosynthetic activity, as well as gas availability into the aquatic ecosystem is observed .
Different physical and chemical methods are developed for decolorization of dyes from the pollutant sites which includes absorption through activated carbon, flocculation, ion exchange, membrane filtration, etc. but due to high expensive, inefficient, and these methods also release different types of wastes which are also toxic for nature . On the other hand, mushroom-based decolorization and degradation of dyes got much more research attention because it is less expensive and eco-friendly and also produces negligible amounts of wastes . Due to the powerful enzymatic activities as well as high adaptability under physically harsh conditions, microbial decolorization and biodegradation is one of the most focused research areas for sustainable developments . Aromatic amines, phenolics, etc. are some intermediates generated during decolorization processes which are highly toxic with low biodegradability as compared to dye. Sometimes, such intermediates inhibit the decolorization ability of bacteria. While fungi have the ability to degrade complex organic compounds and the intermediates through their extracellular enzymes secretion. On the other hand, it is thought that the large surface area of fungi has a greater ability for biodegradation . The decolorization ability of dyes varies from strain to strain, and most of the studies are confined to single-strain-based degradation or decolorization of specific dyes. However, industrial effluents are a cocktail of various organic and inorganic pollutants. Considering these factors, the researcher proposed that the use of novel microbial consortium in the field of bioremediation could be a better option . And many reports suggest that the microbial consortium possesses greater biodegradation ability due to their interactive effect with the contaminants [13, 14, 15]. According to Forgacs et al., the individual strain of a consortium has a specialized role for specific contaminants and may attack the different portions of dye and also has the ability to degrade the intermediate components such as phenolics and aromatic hydrocarbons produced by co-strains . By using this approach, several components of the contaminants can be treated at the same time.
Biodegradation and decolorization ability of mushroom has shown a promising approach since the 1980s. There are many reports regarding the decolorization of different types of dyes by using mushrooms. Cripps et al. reported the decolorization of azo dyes by ligninolytic enzymes secreted by
3. Mechanism of mushroom-based decolorization of dyes
A slight change in additional organic molecules without changing the main structure of the compounds.
Fractionation of the complex structural organic molecules in such a way that the combination of the fractions could give rise to the original molecules.
Mineralization of the complex structural molecules, i.e., transformation of the complex molecules into the mineral forms.
It is thought that adsorption of the dyes is the primary mechanism of dye decolorization by fungus or any other biological mode of decolorization. In many reports, it was found that adsorption of dyes is the important mechanism of dye decolorization by which the transformation of dyes starts [1, 28]. Microscopic observation of the fungal cells showed that instead of fungal hyphae, fungal spores are the main dye-absorbing components . Hydrophilic and hydrophobic interactions of fungi and dyestuff play a crucial role in the enhancement of dye absorption . Some reports also state that when the concentration of extracellular enzymes and cell mass increased, the dye color in the medium decreased indicating that the decolorization of dyestuff is directly proportional to the cell mass as well as the extracellular enzymes produced by the fungus .
Biosorption is a complex physicochemical method of biological materials that have the ability to accumulate pollutants into cellular components through adsorption, ion exchange, deposition, etc. and plays an important role in dye decolorization by fungi [1, 30]. Fu and Viraraghavan reported that the decolorization of dyes like Disperse Red I, Congo red, Acid Blue 29, and Basic Blue 9 by
Fu and Viraraghavan from their study suggested that the adsorption efficiency could be enhanced by treating the biomass with suitable organic or inorganic molecules like formaldehyde, sulfuric acid, sodium hydroxide, calcium chloride, and sodium bicarbonate and by high temperature. Increase in temperature by autoclaving and chemical treatments by 0.1 N NaOH, 0.1 M HCl, and 0.1 M H2SO4 increased the biosorption. It was found that the physical treatments increased the biosorption rate of the Basic Blue 9 dye by 15 times, whereas chemical treatment with 0.1 M H2SO4 enhanced the rate of biosorption of Acid Blue 29 dye by 2 times. The physical treatment of autoclaving of fungal biomass could change the surface charge, and acid pretreatment enhanced the affinity of anionic dyes to bind with the fungal surface . Arica and Bayramoǧlu also observe the same result by autoclaving
|Serial number||Mushroom used||Name of the dye||Remarks||References|
|1.||Orange II, 10B (Blue), RS (Red)|||
|2.||Mushroom stump wastes are found to play a promising role in the decolorization of wastewater containing dyes released from various industries. Freeze-dried mushroom stumps showed higher decolorization efficiency for basic dyes, while heat-dried stumps showed greater biosorption efficiency for acidic dyes|||
|3.||Malachite green, xylidine||pH plays an important role in dye decolorization. Maximum biosorption was observed at pH 3 for malachite green, while, for xylidine, the pH values varied from pH 3 to 4|||
|4.||Malachite green||Biosorbant dose, time, and pH were important factors for biosorption. Ca+ and Na+ ions play a crucial role in biosorption. The presence of hydroxyl, carboxylic acid, phosphate, and amino group on the surface of biosorbent, i.e., |||
|5.||Reactive Red 120||Maximum uptake was noticed at pH 3.0 for all the fungal preparations. And highest dye uptake efficiencies were observed in heat-treated preparations followed by acid-treated, native, and base-treated preparations|||
|6.||Reactive Blue 49 (RB49)||Mix culture of |||
|7.||Methylene Blue||The biosorption ability of a white-rot fungi |||
|8.||Crystal Violet-Brilliant Green||Thermodynamic studies show that Crystal Violet-Brilliant Green adsorption by |||
|9.||Reactive Red 45||Biosorption of Reactive Red 45 by |||
|10.||Disperse Red 60||Immobilized fungal biomass of |||
Biodegradation is the process by which complex organic molecules are converted into its simpler forms by the action of enzymes secreted by fungi, bacteria, or any other living microorganisms . A lot of studies have been carried out to understand dye degradation by mushrooms and the enzyme produced by them, and these are represented in Table 2 . Many reports suggested that the extracellular enzymes produced by mushroom have a potential role in dye decolorization and also have degradability for non-polymeric compounds like polyhydroxy aromatic hydrocarbons, nitrotoluene, and pentachlorophenol under in vitro conditions . In recent years, degradation of polymeric compounds like plastics by various types of mushrooms is also reported .
|Serial number||Mushroom used||Name of the dye||Enzyme produced||References|
|2.||Remazol Brilliant Blue R||Manganese peroxidase, manganese-independent peroxidase, and phenoloxidase|||
|3.||Coralene Golden Yellow, Coralene Navy Blue, and Coralene Dark Red||Laccase, manganese-dependent peroxidase (MnP), and lignin peroxidase|||
|4.||Remazol Brilliant Blue R, Congo red, Methylene Blue, and ethyl violet||Laccase and manganese peroxidase|||
|5.||Acetyl Yellow G (AYG), Remazol Brilliant Blue R or Acid Blue 129 (AB129)||Dye-decolorizing peroxidase (DyP)|||
|6.||Poly-478 and Remazol Brilliant Blue R||Manganese peroxidase|||
|8.||Synthetic dye||Laccases, lignin peroxidases|||
|9.||Blue CA, Black B133, Corazol Violet SR||Laccases|||
|10.||CK, Congo red, Trypan blue, methyl green, Remazol Brilliant Blue R (RBB), methyl violet, ethyl violet, and Brilliant Cresyl Blue||Laccases|||
|12.||Synthetic dye||Ligninolytic enzymes|||
The degradation of polycyclic aromatic carbons by cleaving a carbon-carbon single bond is an important feature of white-rot fungi . The lignin-degrading enzymes of white-rot fungi such as lignin peroxidases or ligninases have a potential role to initiate oxidative depolymerization of lignin for degradation of various organo-pollutants. The ligninolytic activity of a white-rot fungi
4. Degradation of agricultural pesticides, chemical, and other wastes
Nowadays, agricultural management practices depend on the efficient management of biotic factors such as insects, pests, various diseases, weeds, etc.; otherwise, plant growth and development along with crop yields would decrease drastically. To minimize such drastic loss in crop production, there is continuous use of insecticides, pesticides, weedicides, and chemical fertilizers constantly releasing xenobiotic compounds into the environments [19, 57]. Xenobiotic compounds are not easily degraded by microbes and hence remain active in the soil and water . The use of pesticides in India is considerably increasing after 2009–2010. It was reported that the consumption of pesticides in 2014–2015 is 0.29 kg/ha which is 50% higher than that in 2019–2010 . According to previous reports, only 5% of the applied pesticides are effective in targeted pest management, and the rest of the pesticides are mixed with soil and water, affecting human health by interfering with the food chain [60, 61]. On the other hand, modernization, industrialization, and other anthropogenic activities continuously releasing wastes containing hazardous compounds like heavy metals, dyes, phenolic compounds, polyhydroxy aromatic hydrocarbons, etc. are also disturbing agricultural lands .
5. The mechanism involved in the degradation of agrochemicals and other wastes by mushroom
Mushroom-based degradation of agrochemical wastes, heavy metals, phenolics, polyhydroxy aromatic hydrocarbons, and other wastes basically involves enzymatic degradation, biosorption, and bioconversion techniques. Researchers have published a number of research articles on mushroom-based biodegradation of agronomic wastes [19, 62].
5.1. Enzymatic degradation of agricultural wastes
Mycologists and environmental researchers are giving attention to enzymatic degradation of agricultural wastes by using mushrooms. However, the proper role of enzymes in pesticide degradation is not clear. But there is some evidence suggesting that lignin-degrading enzymes are responsible for pesticide degradation. Mushrooms do not secrete pesticide-degrading enzymes in a similar manner; that is, it varies from species to species, type of condition, and other physical and chemical factors [5, 19]. Xenobiotics are chemical compounds that are found in the environment but not naturally produced in the environments. Sometimes, a naturally occurring component is also called xenobiotic when it is excessively available in the environment. Xenobiotics are not easily degradable in nature and hence actively present into the environment. Polycyclic aromatic hydrocarbons, alkanes, oil spills, azo dyes, antibiotics, dioxins, polychlorinated, chlorinated, polyaromatic compounds, etc. are major xenobiotics continuously released into the environment . Microbes play a significant role in the field of biodegradation. There are some reports suggesting the involvement of mushroom fungi in the degradation process of agrochemicals such as xenobiotics, heavy metals, and other agricultural wastes by secreting various enzymes like oxidoreductases, laccases, oxygenase, and peroxidases. These enzymes can degrade the hazardous compounds by the breakdown of ester, amide, ether bonds, and sometimes the aromatic ring or the aliphatic chains of those compounds [6, 19, 64]. The concentration of hazardous compounds, reaction conditions, and the suitable sites are also responsible for the degradation of such compounds. Sometimes, xenobiotic compounds are utilized by mushrooms for their growth and development as their source of energy, carbon, nitrogen, and sulfur . Some researchers reported that the ligninolytic enzyme produced by mushrooms can degrade the PAHs into mineral forms. For example, the ligninolytic enzyme produced by
|Serial number||Mushroom used||Name of the pollutant||Enzyme produced||References|
|2.||2,4-Dichlorophenol||Ligninolytic enzyme-derived vanillin|||
|3.||Radioactive cellulosic-based waste||Ligninolytic enzymes|||
|4.||Malachite green||Biosorption and enzymatic degradation|||
|6.||PAHs||Laccase, manganese-dependent peroxidase, and lignin peroxidase|||
|7.||Anthracene||Lignin peroxidase, laccase, and manganese peroxidase|||
|9.||Crude oil||Ligninolytic enzymes|||
|10.||Crude oil||Ligninolytic enzymes|||
|11.||PAHs, PCBs||lignin-degrading enzyme|||
|12.||PAHs, TNT, bisphenol, dimethyl, phthalate||Laccase, lignin peroxidase, manganese peroxidase, versatile peroxidase|||
|14.||DDT, PHAs, PCBs,||Lip, MnP|||
|15.||PAHs||Peroxidases (LiP, MnP)|||
|16.||Malachite green dye||Ligninolytic enzymes|||
|17.||Lignin, polycyclic aromatic hydrocarbons, polychlorinated biphenyl mixture, and a number of synthetic dyes||Ligninolytic enzymes|||
|19.||Fluoranthene||Manganese peroxidase (MnP) and laccase|||
As there are so many reports on enzymatic degradation of xenobiotics, there are also some reports on the non-ligninolytic degradation of xenobiotics. Jackson et al. reported the degradation of 2,4,6-Trinitrotoluene (TNT) by
5.2 Bioconversion of agricultural wastes
Agro-industrial wastes are the by-products of agricultural processing industries such as grain milling industries, oilseed-processing industries, brewery industries, and fruit and vegetable processing industries. These agro-industrial wastes are rich in various nutrients and bioactive compounds. Nowadays, researchers employ attention in bioconversion of such agro-industrial wastes into some other useful components . Mushroom cultivation on agro-industrial wastes is one of the most important examples of bioconversion where fruiting bodies are used as a product . The choice of agro-industrial substrates depends upon the availability of the substrates . Mushroom cultivated on agro-industrial wastes is mentioned in Table 4 . As agro-industrial wastes are rich in nutrients, mushroom-based mycoremediation of such components gives rise to protein-rich fruiting bodies by degrading such industrial wastes ( Figure 1 ) .
|Serial number||Mushroom species||Agro-industrial wastes||Results||References|
|1.||Banana leaves||Improved yield and provide sustainable feed for ruminant animals|||
|2.||Wheat straw||Bioconversion of wheat straw by synthesizing lignocellulosic enzymes and increased yield.|||
|3.||Wheat straw, rice straw, corn stover, corncobs, sugarcane bagasse (SCB), and banana stalk (BS)||Bioconversion by producing ligninolytic and cellulolytic enzymes|||
|4.||Cotton waste, rice straw||Lipase, peroxidase, cellulase, carboxymethylcellulose enzyme activity increased|||
|5.||Rice straw, banana stem, sorghum stalk||Yield increased, degradation of lignin was observed|||
|6.||Paper waste, cardboard industrial waste||High protein content was observed|||
|7.||Sawdust||Temperature and pH are important factors for the growth of mushrooms|||
|8.||Sawdust||Enzyme activity was measured, and high cellulosic activity is responsible for bioconversion|||
|9.||Paper waste, cardboard waste||Basidiocarps are grown and having high nutrients with no genotoxicity|||
|10.||Rice straw, cocoyam peels||Yield improved with high protein content, fat content|||
|11.||Eucalyptus waste||Successful bioconversion by lignin degradation was observed. Qualitative and quantitative changes are also noticed|||
|12.||Wheat straw||Bioconversion of wheat straw and production of lignocellulosic enzymes are observed|||
6. Biosorption of heavy metals
Biosorption is defined as “the ability of biologically active i.e. living or inactive i.e. non-living or dead organisms/materials that can accumulate and concentrate heavy metals even from very dilute medium by means of adsorption, absorption, ion-exchange or by using metabolic processes” . Biosorption is a complex process, depending upon different factors like temperature, pH, the concentration of the substrates, nature of the substrates, contact time, as well as the property of the host, i.e., cell wall composition, types of proteins, amino acids, lipids, etc. [8, 19, 94].
In recent years, mushroom-based biosorption for waste management is one of the important research interests. A lot of research is going on regarding mushroom-based bioremediation for the cleanup of the environment, and mushroom-based biosorption of heavy metals is an important one . Few reports on mushroom-based biosorptions of heavy metals are mentioned in Table 5 .
|Serial number||Mushroom species||Pollutants||Results||References|
|1.||Heavy metals||Absorb heavy metals from contaminated sites|||
|2.||Cadmium||Mushrooms are grown on the substrate containing cadmium and absorbed cadmium by the fruiting bodies|||
|3.||Heavy metals||Mushroom species are grown in soil by mixing heavy metals and played an efficient role in bioabsorption|||
|5.||Copper, zinc, iron, cadmium, lead, nickel||Mushroom species are grown in aqueous wastes containing heavy metals (copper, zinc, iron, cadmium, lead, and nickel and were found as an efficient bioabsorbant|||
|6.||Copper||Mushroom species showed efficient bioabsorption of copper|||
Lactarius piperatu has higher cadmium removal efficiency
7. Factors affecting the degradation
Biodegradation by means of mushroom basically depends on the survival and multiplication of mushrooms . Different intrinsic and extrinsic factors play an essential role in mushroom survival and multiplication. Substrate concentration, source of nitrogen, carbon-nitrogen ratio, pH, moisture, minerals, particle size, spawning level, surfactant, etc. are important intrinsic factors, while temperature, humidity, luminosity, air composition, etc. are extrinsic factors . Alteration on those factors largely affects mushroom multiplications, and ultimately mushrooms will be unable to survive [102, 103].
pH plays an essential role in mushroom growth and it varies from mushroom to mushroom. Bellettini et al. reported the pH value of 4.0–7.0 helps mycelium growth, while pH value between 6.5 and 7.0 helps basidiocarp development . Velioglu and Ozturk Urek reported that pH of 6.0 gives better growth of
Moisture is another important factor for mushroom growth as the flow of moisture helps to transfer nutrients from mycelium to fruiting body . High moisture contents cause difficulties for mycelium respiration and interpretation of the development of the fruiting body, while low moisture contents lead to the death of the fruiting body . Chang and Miles reported that 50–75% of moisture contents are suitable for the growth of mushrooms .
8. Advantages of mushroom-based mycoremediation
Mushroom cultivation under suitable conditions can help to detoxify various types of contaminants by secreting different types of nonspecific enzymes . Hyphae help to establish direct contact with the contaminants . Mushroom-based bioremediation of pollutants have several advantages, which are mentioned below:
Due to the low cost, it got much more public acceptance.
Safe and eco-friendly technique.
Easy and simple cultivation process.
Mushrooms grow faster and produce reusable end products.
9. Limitations of mushroom-based degradation
The role of mushrooms in bioremediation of environmental pollutants like industrial wastes containing dyes; heavy metals; agrochemical wastes like pesticides, herbicides, insecticides, and other xenobiotic compounds; and agro-industrial wastes like brewery wastes, grain milling wastes, and fruits and vegetable processing wastes are studied . However, certain drawbacks are noticed in mushroom-based remediation. Fungi-based degradation of pesticides is a slower and incomplete process; accumulation of incomplete substrate produces various secondary metabolites that might be harmful . Adaptation of the chosen mushroom species against the pollutant is another major problem of mushroom-based bioremediation . Physicochemical properties of soil and climatic conditions are sometimes problematic for bulk transfer of mushroom under field conditions . The use of mushrooms with beneficial bacterial strain could help to degrade pollutants at faster rates. Identification of genes that are responsible for biodegradation of pollutants and the introduction of such genes to the indigenous strain could solve the availability of capable strain under field conditions . Mushrooms are famous due to their richness in proteins and flavors and also for their medicinal importance. Mushrooms cultivated into the contaminated sites or cultivated for remediation of pollutants can accumulate different types of toxic substances into their fruiting bodies. Consumption of those mushrooms could cause major health problems and sometimes may become the reason for death .
10. Conclusion and future prospective
Logical advancements are considered as key components for the progression of underdeveloped countries. But the majority of industries do not have a legitimate waste treatment plan and discharge an enormous amount of effluents. The accomplishment of a microbial procedure for color removal from the industrial discharge relies upon the usage of microorganisms that viably decolorize manufactured colors of various compound structures. Most of the mushroom-based degradation/decolorization of dyes and other wastes has been performed under laboratory conditions. The outcomes gotten for the most part from the research facility tests rely upon explicit development and optimization of medium, proper handling of mushroom species, and biomass. Therefore, essential works on the topic are still under investigation to assess the information on the process of implementation under field conditions. Certain species of mushroom showed efficient degradation/decolorization/mineralization of the dyes, organo-chemicals, and other industrial wastes either by biosorption or by enzymatic secretion. Based on those facts, proper design of the waste management process by using proper strain is an essential step. The inhibition of growth and secretion of degrading enzymes of mushrooms by the contaminants containing different form of hazardous pollutants is another major problem in mushroom-based degradation of dyes or other pollutants. Utilizing molecular tools for identification of the genes responsible for the degradation of specific dyes may be helpful for biodegradation. Genetic engineering technologies for the development of genetically modified strain for the degradation or decolorization could solve the problem. The connection among the researchers of interdisciplinary research fields like biotechnology, microbiology, chemistry, and genetic engineering could help to develop a successful technique for bioremediations.