Open access peer-reviewed Edited Volume

Next Generation Sequencing

Advances, Applications and Challenges

Next generation sequencing (NGS) has surpassed the traditional Sanger sequencing method to become the main choice for large-scale, genome-wide sequencing studies with ultra-high-throughput production and a huge reduction in costs. The NGS technologies have had enormous impact on the studies of structural and functional genomics in all the life sciences. In this book, Next Generation Sequencing Advances, Applications and Challenges, the sixteen chapters written by experts cover various aspects of NGS including genomics, transcriptomics and methylomics, the sequencing platforms, and the bioinformatics challenges in processing and analysing huge amounts of sequencing data. Following an overview of the evolution of NGS in the brave new world of omics, the book examines the advances and challenges of NGS applications in basic and applied research on microorganisms, agricultural plants and humans. This book is of value to all who are interested in DNA sequencing and bioinformatics across all fields of the life sciences.

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Next Generation SequencingAdvances, Applications and ChallengesEdited by Jerzy Kulski

Published: January 14th 2016

DOI: 10.5772/60489

ISBN: 978-953-51-2240-1

eBook (PDF) ISBN: 978-953-51-5419-8

Copyright year: 2016

Books open for chapter submissions

47263 Total Chapter Downloads

44 Crossref Citations

61 Web of Science Citations

96 Dimensions Citations


Open access peer-reviewed

1. Next-Generation Sequencing — An Overview of the History, Tools, and “Omic” Applications

By Jerzy K. Kulski


Open access peer-reviewed

2. Next Generation Sequencing in Aquatic Models

By Yuan Lu, Yingjia Shen, Wesley Warren and Ronald Walter


Open access peer-reviewed

3. MHC Genotyping in Human and Nonhuman Species by PCRbased Next-Generation Sequencing

By Takashi Shiina, Shingo Suzuki and Jerzy K. Kulski


Open access peer-reviewed

4. Transcriptomic Profiling Using Next Generation Sequencing - Advances, Advantages, and Challenges

By Krishanpal Anamika, Srikant Verma, Abhay Jere and Aarti Desai


Open access peer-reviewed

5. Computational Analysis and Integration of MeDIP-seq Methylome Data

By Gareth A. Wilson and Stephan Beck


Open access peer-reviewed

6. Analysis of Next-generation Sequencing Data in Virology - Opportunities and Challenges

By Sunitha M. Kasibhatla, Vaishali P. Waman, Mohan M. Kale and Urmila Kulkarni-Kale


Open access peer-reviewed

7. RNA-seq – Revealing Biological Insights in Bacteria

By Mariana P. Santana, Flavia F. Aburjaile, Mariana T.D. Parise, Sandeep Tiwari, Artur Silva, Vasco Azevedo and Anne Cybele Pinto


Open access peer-reviewed

8. Dealing with the Data Deluge – New Strategies in Prokaryotic Genome Analysis

By Leonid Zaslavsky, Stacy Ciufo, Boris Fedorov, Boris Kiryutin, Igor Tolstoy and Tatiana Tatusova


Open access peer-reviewed

9. Reaping the Benefits of Next-generation Sequencing Technologies for Crop Improvement — Solanaceae

By Sushil Satish Chhapekar, Rashmi Gaur, Ajay Kumar and Nirala Ramchiary


Open access peer-reviewed

10. Perspectives on the Application of Next-generation Sequencing to the Improvement of Africa’s Staple Food Crops

By Melaku Gedil, Morag Ferguson, Gezahegn Girma, Andreas Gisel, Livia Stavolone and Ismail Rabbi


Open access peer-reviewed

11. Hop-variety Identification Using First- and Second-generation Sequencing

By Hiromasa Yamauchi


Open access peer-reviewed

12. Analysis of Haplotype Sequences

By Sally S. Lloyd, Edward J. Steele and Roger L. Dawkins


Open access peer-reviewed

13. On Genotyping Polymorphic HLA Genes — Ambiguities and Quality Measures Using NGS

By Szilveszter Juhos, Krisztina Rigó and György Horváth


Open access peer-reviewed

14. DNA-based Diagnosis of Uncharacterized Inherited Macrothrombocytopenias Using Next-generation Sequencing Technology with a Candidate Gene Array

By David J. Rabbolini, Marie-Christine Morel Kopp, Sara Gabrielli, Qiang Chen, William S. Stevenson and Christopher M. Ward


Open access peer-reviewed

15. Clinical Implementation of Next-generation Sequencing in the Field of Prenatal Diagnostics

By Gwendolin Manegold-Brauer and Olav Lapaire


Open access peer-reviewed

16. Impact of Gene Annotation on RNA-seq Data Analysis

By Shanrong Zhao and Baohong Zhang


Edited Volume and chapters are indexed in

  • Worldcat
  • OpenAIRE
  • Google Scholar
  • AZ ebsco
  • Base
  • CNKI

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