Block copolymer (BCP) composed of two (more) immiscible polymers form variety structures with the periodicity of several tens nanometer both in bulk and thin films. BCP thin film has attracted great attention as an applicable material to various fields, e.g., solar cell [1–3], nanolithography [4–6], and size-selective separation [7, 8]. In bulk state, microphase-separated structure is predicted by the Flory-Huggins interaction parameter, the degree of polymerization, and the volume fraction of blocks , whereas in thin film, film thickness [10, 11] and substrate-polymer interaction and/or also polymer-air interaction must be taken into consideration. Controlling morphology, orientation and size of the structures are necessary for practical use although phase-separation behavior of BCP in thin film becomes more complicated. This has motivated numerous orientation control methodology studies that have examined the influence of film thickness [10, 11, 13, 14], surface/or interfacial free energy [14–17], surface topology [15, 18, 19], external applied fields (shear-induced [4, 20], electric field , magnetic field , and light-driven [23, 24]), solvent vapor or thermal annealing[25–30], and directional solidification [31–33]. Since functionality and physical property are also strongly related to the structure and the mobility in the vicinity of interface, revealing structure in detail is required. Suitable characterization techniques are required to monitor the structures of BCP both laterally and in-depth. Several approaches have been used to find BCP structures. Atomic force microscopy (AFM), electron microscopy, dynamic secondary ion mass spectrometry (DSIMS), X-ray photoelectron spectroscopy (XPS), grazing incidence small angle X-ray or neutron scattering (GISAXS, GISANS), X-ray or neutron reflectivity (XRR, NR), etc. have been used to study the structure of BCP thin films. AFM can enable an access of the information only near the surface although the surface structure can be directly observed and easily understandable. Electron microscopy is a powerful tool for visually examining a cross-sectional view of polymeric thin films in two-and three-dimensional real space . DSIMS can elucidate the BCP morphology and the self-diffusion of polymer chains in thin films along a depth direction . Time-of-flight (ToF) SIMS using ion cluster beam was reported to be a particularly well-suited technique that enables the in-depth profiling of polymers [36, 37]. X-ray photoelectron spectroscopy depth profiling with C60+ sputtering revealed the ion distribution in lithium salt-doped BCP thin films [38, 39]. Electron microscopy, DSIMS, and XPS techniques are essentially accompanied by the destruction of specimen because of processing such as sectioning or etching for analysis. In particular, it is important to take into account the deformation and losing of a precise original spatial coordinate induced by sectioning and chemical reactions induced by etching in the analysis of results obtained by these techniques. In contrast, neutron reflectivity (NRR) measurements enable a practically nondestructive analysis of depth profiles and ordering of microphase-separated structure in BCP thin films [40, 41]. However, the NRR provides structural information (density profile) only in the vertical direction to the sample surface and lateral information of the structure is inaccessible. In addition, accuracy of the density profile (depth-resolved information) perpendicular to the surface becomes worse when the film thickness is large for analyzing periodicity of microphase-separated structure. Generally, NRR depth profiling is suited for very thin film (less than 100 nm) as in the above case. GISAXS is another very powerful tool for understanding the nanostructure in both vertical and lateral directions of organic thin film (BCP thin film). And GISAXS is essentially nondestructive method under the condition of the no radiation damage of X-rays [42–47]. Commonly, SAXS and GISAXS methods have been conducted using hard X-rays with energy range of 6–14 keV. However, under these conditions, the penetration depth of X-rays rapidly reaches the thickness scale of the organic materials in the vicinity of the critical angle of total reflection at the polymeric surface, which is making depth-resolved GISAXS measurements with hard X-rays totally impractical. A depth-sensitive GISAXS method using tender X-ray (1.77 keV) was first reported for the BCP thin film by Okuda et al.  and Wernecke et al. . They investigated the structural relaxation near the surface and the dynamic heterogeneity of polymer chains in thin films. At even lower X-ray photon energies, near the adsorption K edges of the polymeric materials (the oxygen, nitrogen, and carbon K edges), the fine structure of the adsorption edge can be utilized in GISAXS as reported by Ruderer et al. . The grazing-incidence resonant soft X-ray (GI-RSoXS) has been applied for polymer blend thin films with low contrast in the real part of the refractive index for the hard X-rays but with significant differences in the soft X-ray regime. Furthermore, the X-ray penetration depth is drastically affected by the changes in the X-ray photon energy across the K-edge. The surface-and volume-sensitive structure of polymer blend films had been analyzed using this technique . Similar to the GISAXS, GISANS has been developed by Müller-Buschbaum and co-workers. GISANS is a perfectly nondestructive approach for structure analysis and has essentially the same capability for surface-sensitive , interface-sensitive (structural information near the polymer-substrate interface enabled by the ability of the neutrons to go through the substrate) [52, 53], and depth-sensitive analysis . Moreover, in time-of-flight mode GISANS (ToF-GISANS) [52, 55] a broad wavelength band is used instead of a single neutron wavelength, i.e., a range of different scattering vectors is directly probed by the measurement under a fixed angle of incidence. At an appropriate incident angle, it is possible to simultaneously conduct surface- and bulk-sensitive measurements. While GISANS possesses advantages as compared with the GISAXS, GISANS experiments still remain very rare because GISANS requires very high-flux sources to measure the much weaker signals in grazing-incidence geometry and the need for deuterium labeling (in some cases, of course, this is beneficial for structure analysis by tuning the contrast). These GISANS techniques have been well summarized in the reviews .
In this chapter, recent advanced GISAXS experiment utilizing low-energy X-rays will be introduced. GISAXS probes the complex nano- and microphase-separated structure in polymer thin films. Especially, tuning the energy of GISAXS in the tender and soft X-ray regime allows to the tailoring of X-ray penetration depth and contrast and thereby the probing of more complex morphologies in polymer thin films. GI-RSoXS has been applied for polymer blend thin films with low contrast in the real part of the refractive index for the hard X-rays but with significant differences in the soft X-ray regime. Furthermore, the X-ray penetration depth is drastically affected by the changes in the X-ray photon energy across the K-edge. The surface- and volume-sensitive structure of polymer blend films had been analyzed using this technique.
2. Grazing-incidence small angle X-ray scattering
Incident X-ray beam goes into the sample surface at a very shallow angle (normally less than 1°). Scattering is measured with a two-dimensional detector as a function of the exit angle (out-of-plane angle) and the in-plane angle . The magnitude of the scattering vector is given by q = 4πsinθ/λ(λ: wavelength of X-ray, 2θ: scattering angle). The scattering vector qz means the component vertical to the film surface. The qx and qy are the components of scattering vectors in the sample surface, perpendicular to and directed to the X-ray beam, respectively. For each data set I(y, z), pixels of the detector were converted into exit angle normal to the sample surface and a scattering angle parallel to the surface by simple geometrical consideration. The amplitude of scattering vector q is composed of qx, qy, and qz related to the experimental angles by
As shown in Figure 1, typical sample-to-detector distances (SSD) for GISAXS are of the order of 1–2 m. In the case of small angle scattering, the two-dimensional detector probes mainly the qy and qz information because qx is very small and the curvature of the Ewald sphere is negligible. The calculation of the out-of-plane scattering vector is considerably complex. Above critical angle of polymeric materials, the theoretical penetration depth is much larger than the film thickness (order of micro-meter) (when hard X-ray is normally used) as shown in Figure 2. The transmitted wave can therefore be reflected at the polymer-substrate interface in combination with diffraction from the structures in the thin film. Under the assumption that each X-ray scatters no more than once from the objects and there is no transmission through the substrate, there are four possible scattering events to happen, additionally refraction at the sample surface (air-polymer interface) (Figure 3).
3. Depth-resolved structure analysis of microphase-separated structures in block copolymer thin film by grazing-incidence small angle X-ray scattering utilizing tender X-ray
3.1. Cylindrical microdomain in block copolymer thin film 
In this section, GISAXS measurement with low energy (tender) X-ray (2.40 keV) is introduced in order to precisely elucidate the depth profile of a microphase-separated structure (hexagonally packed cylinders) of a polystyrene-b-poly (2-vinylpyridine (S2VP) thin film on a silicon wafer with the cylindrical microdomains (poly (2-vinylpyridine): P2VP) oriented parallel to the substrate after the appropriate thermal annealing in vacuum. The cylindrical domains in the S2VP thin film were preferentially oriented parallel to the surface of the substrate induced by the surface free energies and/or an interfacial interaction between S2VP and the substrate. In GISAXS, the structural parameters of the cylindrical domains in both the lateral and vertical directions are accessible because the diffraction spots appear with the offset in the qy direction.
S2VP thin film (number average molecular weight Mn = 26,400, molecular weight polydispersity index = 1.24, and ϕPS = 76.3 vol%) was prepared by spin casting from toluene solution (10 wt%) of S2VP onto a silicon wafer substrate at 3000 rpm for 30 s. Subsequently, the S2VP thin film was thermally annealed under vacuum at 170 °C for 48 h. The sample surface was composed of PS component (X-ray photoelectron spectroscopy and water contact angle , predicted by surface free energies of components ) was a very flat and smooth examined by atomic force microscopy and optical white-light interferometer microscopy measurements.
Tender X-ray GISAXS measurement (room temperature) was performed at BL15A2  at the Photon Factory, KEK, Tsukuba in Japan. The BL15A2 is an undulator beamline where X-rays in a wide energy range from 2.1 to 15 keV (energy resolution is 2 × 10−4) is available. In this study, the energy of X-ray was set at 2.40 keV (the wavelength of 5.16 Å) and the sample-to-detector distance (SDD) was 830 ± 5 mm. The accuracy of the camera lengths arises from the scattering vector calibration on a detector with a standard specimen and a footprint of the incident beam on the sample surface (sample size of c.a.1 cm). The X-ray incident angle was varied between 0.290° and 0.620° and PILATUS 2M designed for usage in vacuum was used as a detector for the 2D scattering pattern. X-ray exposure time of 300 s was sufficient to obtain a clear scattering pattern. Hard X-ray (wavelength 1.0 Å) GISAXS measurements were performed at BL10C in Photon Factory and BL03XU57 in SPring-8, Harima, Japan using PILATUS 2M and CCD (Hamamatsu Photonics) detectors with SDD of 2.3 m. All detectors were calibrated using lead stearate prepared in-house (d = 5.01 nm, calibrated).
The X-ray penetration depth Λ is defined as the depth at which the X-ray intensity is attenuated by 1/e. The value of Λ depends on X-ray energy (wavelength λ), the critical angle, , of total reflection, and the incident angle . Surface roughness influences practically the penetration depth of X-rays because various are provided. The roughness of the surface used here is regarded as sufficiently small to estimate the penetration depth as evidenced by the clear observation of the critical angle in XRR. Under experimental conditions with the ideally flat surface, is given by
where is the imaginary part of the complex refractive index. The critical angle is specified as where δ is deviation from the real part of the refractive index, and are given by
where re is the classical electron radius (2.82 × 10−5 Å), is Avogadro’s number, is the mass density, is the fraction of element Z, is the relative atomic mass, is the nonresonant term of the atomic scattering factor corresponding to the atomic number, and and are the real and imaginary parts of the anomalous dispersion for the incident X-ray energy E, respectively. For example, here we used 4.1468 × 10−5 for and 7.0239 × 10−7 for of PS at 2.40 keV. The value of S2VP thin film using GISAXS and XRR measurements was obtained. The calculated S2VP penetration depth is shown in Figure 2. It is hard to precisely control the penetration depth at the nanometer scale for GISAXS experiment conducted using hard X-rays (8–12.4 keV) because the value of rises steeply at . On the other hand, as the X-ray energy decreases, changes more gradually near the critical angle and shows decreased depth values at angles even greater than. Hence, better control of is expected for depth-resolved GISAXS measurements using tender X-ray (2.40 keV) because of the critical angle and attenuation coefficient values that are much greater than those for the hard X-rays.
GISAXS measurements of the S2VP thin film (thickness of 420 nm) using tender X-ray were performed at various incident angles and many Bragg spots were measured as shown for large in Figure 4. All spots were assigned to parallel oriented hexagonally packed cylinders. GISAXS patterns at approximately qy of 0.26 nm−1 are presented in Figure 4(c) and (d) and show a remarkable elongation of the Bragg spots in the qz direction for smaller. One-dimensional scattering profiles vertically cut at qy = 0.26 nm−1 with various incident angles are shown in Figure 5. Bragg peaks were assigned to the scattering from transmitted (denoted by T) and reflected (denoted by R) beams by the substrate. These two scattering events are typically noticeable in GISAXS measurements [45, 46]. The second-order peaks derived from (11) reflection at qz approximately 0.6 and 0.7 nm−1 were used for structure analysis because the primary peak from the (10) plans was partially invisible due to the detector gap. The magnitudes of the Bragg spot full widths at half maximum (FWHM) varied in the vicinity of the, with larger FWHM values observed at smaller incident angles.
The observed peak broadening can be interpreted by the change in the penetration depth. While generally such broadening can be understood by either the grain size effect and/or disordering of the crystal lattice, the FWHM in the qy indicated no change irrespective of the incident angles as shown in Figure 6, eliminating the influence of the lattice disordering because the broadening was mainly seen in the qz direction and the size effect was dominantly considered. Rather, the broadening in this case is because of the reduction in the size of the observed region. The FWHM of a scattering peak depends on the grain size of a crystal, as expressed by the Laue function, L(q)
where N is the number of the reflection plane and b is the unit lattice vector related to z-direction normal to the surface. Here, the X-ray wave decays exponentially, and considering attenuation decay, the Laue function can be re-expressed as
where is the periodicity of the (01) plane. Since the scattering intensity is proportional to the square of the Laue function, the FWHM can be calculated simply. The FWHM of the Bragg spots of the T (11) plane in qz direction experimentally obtained is shown in Figure 6. The calculated values for FWHM in the qz direction for the penetration depth Λ given by Eq. (2) are also plotted in Figure 6. The change in the calculated width shows the same trend as the experimental results, indicating that the broadening of the Bragg spots can be explained by the size effect determined by the depth Λ. Thus, the observed region of GISAXS measurement can be controlled with the incident angle, enabling depth-resolved GISAXS.
When , X-rays travel on the surface of the film and cannot propagate in the film. Only the evanescent wave can penetrate from the sample surface into the film. In this situation, the scattering peak along the qz direction is observed at the position given by the sum of the incident angle and the true scattering angle derived from the period of the observed structure. Thus, αs can be given as follows:
Using above relation, the true qz value of the (11) spot can be estimated from the experimental peaks. On the other hand, in the case of , an X-ray wave can travel into the film. The X-ray first refracts at the sample surface, goes through the film, is reflected by the interface between the sample and substrate, and finally exits out of the film surface with refraction as shown in Figure 3. Normally, some scattering events in GISAXS experiments occur because of the refracted X-rays at the polymer surface and reflected X-ray on the substrate surface, resulted in appearance of a number of scattering peaks. The scattering cross-section for GISAXS of the block copolymer thin film has been calculated within the framework of the distorted wave Born approximation (DWBA) . Lee et al. , Yoon et al. [44, 45], and Busch et al. [46, 47] introduced the DWBA (or a combination of Bragg’s and Snell’s laws, refraction and reflection) to estimate the scattering peak positions. Scattering intensity due to the incident X-ray (transmission) and reflected X-ray (reflection) were pronounced. Debye-Scherrer rings of the block copolymer films with powder-like orientation of lamellar domains. The scattering peaks arising from transmitted and reflected X-rays at the substrate can be calculated following 
where m represents the peak order, which is 31/2 for the (11) plane in hexagonally packed cylindrical microdomains. The upper (−) and lower (+) branches in the equation indicate the Bragg diffraction of the transmitted and reflected X-rays, respectively. D is the characteristic length of the given plane. As for the (11) plane, Eq. (9) can be derived from Eq. (8) as follows:
where D corresponds to the in this case. When the was set to 18.8 nm, the DWBA calculation Eqs. ( 8) and ( 9) gave the best representation for all Bragg spots as shown by crosses in Figure 7.
For GISAXS experiment in the soft X-ray region, the large curvature of the Ewald sphere may give rise to an apparent distortion of the GISAXS pattern when the measurements are conducted with a fixed angle of incidence and using the area 2D plane detector. Yamamoto et al.  discussed the effect of the Ewald sphere curvature and performed model calculations using DWBA . At the lower energy of 1.77 keV, while the interparticle interference peaks extended and bent inward at large qz, (approximately 2.0 nm−1), no bending of the extended peaks was observed using hard X-rays. In the presence of the Ewald sphere curvature, the unmodified equation is no longer valid. In this study, Eq. (9) that had been developed for the hard X-ray regime to explain the experimental GISAXS pattern is confirmed to be valid for this observed q-range with tender X-ray regime 2.40 keV .
The lattice constant b associated with the direction perpendicular to the surface was slightly smaller than the lateral lattice constant a. The hexagonal lattice was slightly deformed, in particular, the nanocylinders were packed into distorted hexagonal lattice that was laterally elongated and/or vertically collapsed. The distorted hexagonal lattice in polymeric films has been often observed during the drying of solvents . The lattice constant remained almost constant with respect to the depth. In contrast, the constant b and the angle φ between the lattice vectors increased with decreasing depth, i.e., approaching the surface, the lattice deformation was relaxed to a normal hexagonal lattice (Figure 8).
3.2. Orientation and relaxation behaviors of lamellar microdomains of poly(methyl methacrylate)-b-poly(n-butyl acrylate) thin film 
In this section, we investigated the phase-separation behavior of poly (methyl methacrylate-b-n-butyl acrylate) (PMMA-PnBA) forming a lamellar structure aligned parallel to the substrate after appropriate thermal annealing with GISAXS measurement. The structure development through such as degree of the lamellar orientation and relaxation of the lamellar domain spacing was inquired. Also, the GISAXS with tender X-ray for depth-sensitive analysis was conducted to reveal that the difference of the lamellar domain spacing near the surface from the bulk.
To obtain a thin film of the block copolymer PMMA-b-PnBA (Mn = 32,000, Mw/Mn = 1.17, fPMMA = 0.44), PMMA-b-PnBA in toluene (5 wt% polymer solution) was prepared. The thin film was obtained by spin cast on silicon wafer at 3000 rpm for 30 s. The thin films (thickness was 280±30 nm) were dried at room temperature, subsequently thermal annealing was performed at 160° for given time. GISAXS measurement utilizing hard X-ray and tender (soft) X-ray was performed. Hard X-ray GISAXS measurement was conducted at beamlines BL6A and BL10C in Photon Factory of KEK, Tsukuba in Japan and BL03XU in SPring-8, Hyogo in Japan [65, 66] with wavelength of 0.15 (BL6A), 0.1488 (BL10C), and 0.1 nm (BL03XU), respectively. Tender X-ray GISAXS measurement was performed at BL15A2 in Photon Factory.
2D GISAXS (hard X-ray) patterns with various annealing times were shown in Figure 9. The pattern of as-spun sample (Figure 9a) was shaped like an ellipse, which might arise from kinetically frozen or poorly ordered structure. Partially intense scattering was observed at qz of 0.25–0.28 nm−1 where was emphasized due to the so-called Yoneda peak, i.e., it did not indicate specific orientation, suggesting that no orientation of phase-separated structure of PMMA-b-PnBA appeared without thermal annealing. After the sample was thermally annealed for even 1 min, the scattering intensity around qy = 0 (near the beam stop) grew. In addition, two clear ring-shaped scattering patterns like Debye-Scherrer rings were observed. Each scattering ring was arising from transmitted (denoted by T) and reflected (denoted by R) beams as described in previous section. The scattering intensity near beam stop became strong with annealing time. This change in GISAXS pattern indicates the growth of the parallel orientation of the lamellar microdomain. The development of the normalized scattering intensity  from parallel lamellar structure is shown in Figure 10. Orientation is nearly complete after annealing for 60 min. The GISAXS measurement gave structure information about domain spacing of the lamellar morphology. The domain spacing (D) of the lamellar structures aligned parallel to the surface was estimated. To determine the accurate domain spacing, the distorted wave Born approximation (DWBA) was applied for analysis of the GISAXS patterns. The experimentally estimated D values are also plotted as a function of the annealing time in Figure 10. The value of the D approached to the D0 of the bulk sample (independently obtained to be 21.6 nm) with an increase in annealing time. The D of the parallel orientated structure was slightly smaller than D0 even after 4 h thermal annealing, i.e., the spacing collapsed vertically. Consequently, the lamellar structure was deformed along the depth direction (similar phenomena as the previous section). Thermal annealing induced the relaxation of the domain spacing and it seems taking approximately more than 2 h to complete the relaxation of D (equals to the value of the bulk)
As is well known, preferential wetting of surface and substrate interfaces plays an important role of orientation in thin film [12, 17]. In this case, surface energies of PMMA, PnBA, and Si substrate are 41.1, 33.7, and 77.4 ± 0.5 mJ/m2, respectively . According to the surface free energies, it will be predicted that PMMA segregates to the surface of the silicon substrate, whereas PnBA segregates to air surface. As a result of preferential wetting, the parallel orientation of lamellar structure is induced at the surface and/or the polymer/substrate interfaces and the oriented lamellae propagate into the entire film . In fact, XPS measurement proved that surface molar fractions of PnBA (within a few nanometers) were 80 mol% (repeat unit) in as-cast film and the PnBA component perfectly covered on the surface after thermal annealing with only 60 s. The segregation of each component, orientation of the lamellae, and relaxation of the domain spacing occurred in different time scale. It can be concluded that the PnBA first segregated at air surface within a minute after annealing (PMMA may segregated at the interface), second the microphase-separated structure aligned parallel to the surface, followed by relaxation of the domain spacing.
The polymer thin films have reported to have different mobility dependent on the local region, i.e., near the surface, inside, or near the polymer/substrate interface. It is quite intriguing to investigate that the depth dependence of structure difference exists, in other words, whether there are difference between the structure (orientation, morphology, d-spacing, etc.) in the vicinity of the surface and inside of the film, or not. The GISAXS measurements of PMMA-b-PnBA thin film thermally annealed for 2 h with tender X-ray was performed with various incident angles. As shown in Figure 11(a) and (b), in the case of αi < αc, the scattering (marked arrows) of the lamellar structure oriented parallel to the substrate was considerably diffuse and broaden, while in the case of αi > αc, the scattering became clear and sharp. The FWHM values of scattering peak (parallel lamellar domains) in the one-dimensional GISAXS profile obtained vertically cut at various incident angles can be simulated as the same manner of the size effect of measured region as discussed in the previous section (modified the Laue function). Thus, the penetration depth was controlled by changing the incident angle. At near the critical angle, the surface-sensitive measurement is possible as predicted from Eq. (2). The true qz value of the oriented lamellar structure parallel to the substrate is estimated using the experimentally observed peaks, i.e. D near the surface can be estimated. At αi of 0.525° (penetration depth Λ of 32.4 nm), D was obtained to be 21.6 nm which is equal to the D0 value (21.6 nm) of the bulk sample. The value of D near the surface is slightly larger than the value 21.4 nm obtained from DWBA calculation (inside the whole film). This means that relaxation of the domain spacing near the film surface preceded that of the inside. According to previous reports, polymer chain near the surface indicates higher mobility (lower glass transition temperature or viscosity) [68–70]. Moreover, the lamellar structure started to orient from both the air/polymer and polymer/substrate interfaces, which was induced by segregation of one component in the BCP. Therefore, that the faster relaxation of the D of the lamellar structure near the surface was caused by the faster orientation and higher mobility in the vicinity of the surface.
3.3. Evaluation of mesogen orientation in thin films of polyacrylate with cyanobiphenyl side chain 
Understanding the orientation behavior of polymer chain in the vicinity of interfaces (both substrate and free surfaces) is of practical importance in organic thin film technologies such as coating and photoresisting processes. Thus, a large amount of fundamental knowledge has been ever accumulated. It has been broadly recognized that diverse physical properties of polymeric materials in ultrathin film state are very different from those in the bulk state. Compared with the vast amount of studies for amorphous and crystalline (LC) polymers, studies on the anomaly in structure and orientation of side chain liquid crystalline polymers in ultrathin film states are rather unexplored. A large number of data related to mesogen orientation have been reported [72–78]. Accordingly, the side chain LC polymers are mostly aligned homeotropically [23, 79–81] The significant effect of the sample surface is apparent from the fact that the mesogen orientation changes to a planer orientation as the sample surface is covered by another layer or material [23, 82–84]. A cyanobiphenyl(CB)-containing polymethacrylate (PCBMA) exceptionally indicated the planar orientation regardless of the fact that the homologous polyacrylate (PCBA) oriented homeotropically . This unexpected orientation behavior is responsible for the difference in the main chain rigidity (but still no rational explanation). In these contexts, the investigation to reveal in detail the orientation of PCBA is proceeded by the GISAXS measurements by systematically changing the film thickness. Additionally, GISAXS with hard (8.05 keV) and tender (2.30 keV) X-rays were carried out.
The side chain LC polymer PCBA (chemical structure shown in Figure 12, Mn = 12,000, Mw/Mn = 1.83, glassy state – 13°C (Tg: glass-transition temperature)–smectic A - 95°C (Ti: isotropization temperature)) films on quartz plates were prepared by spin-casting from 0.12–3.0 wt % chloroform solutions to make different thickness samples. The spin-cast film samples were annealed at 135 °C, cooled to a target temperature, kept for 10 min, and then subjected to the measurements. The layer spacing of the smectic A of LC polymers in the bulk was estimated to be 4.6 nm (SAXS). GI-SAXS experiments with hard X-rays (Cu Kα radiation (λ = 0.154 nm)) were conducted with a FR-E X-ray diffractometer equipped with two-dimensional imaging plate R-AXIS IV detector (Rigaku). GI-SAXS experiments using low-energy X-rays were performed at BL-15A2  at the Photon Factory, KEK, Tsukuba, Japan. Experimental details of the GI-SAXS measurements were described in previous sections.
Figure 13 indicates GI-SAXS data measured with hard X-rays (λ = 0.154 nm) for 30 nm thick at 80 °C. For 140 nm thick film, the scattering peaks corresponding to d (smectic layer) = 4.6 nm (100) and 2.3 nm (200) were clearly seen in both out-of-plane and in-plane directions. The intensity of the peaks in the out-of-plane direction was significantly small in 30 nm thick film (as peaks was weakly shown in 1D profile), and no peaks were essentially recognized for 15 nm thick film, although those in the in-plane direction were clearly seen. These results evidently indicate the coexistent of planarly and homeotropically oriented CB in the films with film thickness greater than 30 nm, and that the CB mesogens were oriented only planarly at 15 nm thickness.
GI-SAXS measurements with synchrotron tender X-rays (λ = 0.539 nm, 2.30 keV) were achieved at various αi. Figure 14 shows the 2D GI-SAXS images for 30 nm thick film at room temperature. The CB mesogens at this thickness as mentioned before are oriented both in the homeotropic and planar directions (coexistence). The αc in this sample was estimated at about 0.54° for this X-ray energy. Under conditions of αi < αc (αc is about 0.54° for 2.30 keV), the scattering signals in the thin film was observed only the out-of-plane direction as shown in Figure 14a and b, where Λ is estimated as in the range less than 10–20 nm in these experimental conditions. It is apparently indicated that the CB mesogens adopt homeotropic orientation in the free surface region. When αi > αc, the out-of-plane scatterings were split into double peaks in the qz direction as shown in Figure 14c–e. The split double peaks originate from the transmitted X-ray through the film and then reflected on the substrate . Hence, the high qz peak of the double peaks can be assigned to the scattering from the reflection path on the film surface. The split spots means that the X-ray beam actually travelled through the overall film thickness and reached the sample/substrate interface. When αi > αc (Λ > 100 nm), the peaks appeared in-plane direction due to the planar orientation were clearly observed as shown in Figure 14d–f with arrows. These signals undoubtedly originate from the mesogens near the polymer/substrate interface.
From the overall data of UV-vis absorption spectroscopic  and the GISAXS measurements utilizing hard and tender X-rays, the orientation structural models of CB mesogens in PCBA thin films are schematically illustrated in Figure 15. In thick films with 140 nm, the CB mesogens are almost aligned homeotropically. However, a considerable number of the CB mesogens planarly anchored exist near the substrate (polymer/substrate interface) as revealed by GI-SAXS measurements with hard X-rays (Figure 13). At a thickness of 30 nm, the amounts of homeotropically and planarly oriented CB mesogens is comparable, where depth-resolved information is obtained by GI-SAXS with tender X-ray experiments (Figure 14). In the film thickness of 10–15 nm, the CB mesogens adopt almost planar alignment. When the film becomes further thinner from the critical level of 7 nm, the planar alignment near the surface disappears where the liquid crystal structuring (antiparallel packing of the CB mesogens) is lost.
4. Grazing-incidence resonant soft X-ray scattering 
The GI-RSoXS is a novel technique, which is in particular suited for more complex system such as multicomponent block copolymer and polymer blend films. Resonant soft X-ray scattering has already been successful for probing morphology and spatial structure in organic photovoltaic (OPV) systems [85, 86] and triblock copolymer system . GI-RSoXS allows for detecting near surface and inner structure separately at fixed incident angle by tuning X-ray photon energy because the penetration depth of the X-ray beam is drastically affected by the change in X-ray photon energy across the adsorption edge. Adsorption K edge for organic materials composed of mainly carbon, nitrogen, and oxygen are 284, 409, and 543 eV. The fine structure of the adsorption edge can be utilized in GISAXS measurements. Near edge X-ray adsorption fine structure (NEXAFS) spectrum needs to be probed for the polymeric materials in order to estimate the complex refractive index of X-ray that becomes important for depth-sensitive and component sensitive analyses. Typically, the NEXAFS spectra of polymers, which have low contrast in the real part of the refractive index in the hard photon energy regime, indicate considerable differences in the soft X-ray regime. In this section, investigation of nanostructure in the polymer blend thin film, poly(3-hexylthiophene) (P3HT) and poly[5-(2-ethylhexyloxy)-2-methoxycyanoterephtalylidene] (MEH-CN-PPV), is introduced as an example of GI-RSoXS experiment carried out by Ruderer et al. .
GI-RSoXS measurements with soft X-ray were conducted at the synchrotron beamline 22.214.171.124 of the advanced light source (ALS) at the LBNL in Berkeley (USA) [88, 89]. Due to the high adsorption of soft X-ray in air, full setup (sample and X-ray detector) was kept in high vacuum. The energy of X-ray was used in the range of 280–320 eV (λ: 4.4–3.9 nm). Sample-to-detector was 18.5 cm that was sufficient for detecting length scale in the range from 21 nm to a few micron meters. An incident angle αi = 2°, which is near the critical angle αC of 2.3° for 280eV and 1.5° for 283 eV. The both polymers (P3HT and MEH-CN-PPV) were dissolved in chloroform. The thin film of polymer blend was prepared by spin coating from the solution; the thickness was controlled to be about 70 nm. The films were annealed at 200 °C for 10 min in air without degradation. The NEXAFS spectroscopy measurement was conducted for taking the wavelength dependent refractive index (n = 1 − δ + iβ) of polymers used here for electromagnetic radiation near the adsorption edge. NEAXFS spectra were also obtained at the same beam line of the ALS. The sample environment is identical to the GI-RSoXS setup. The polymer thin films were prepared on silicon nitride membranes and measured in transmission geometry. The adsorption part β of the refractive index was obtained through Beer’s law. The real part δ of the refractive index was calculated from the β using Kramers-Krongis relation.
Figure 16a shows the X-ray energy dependence of the dispersion δ and the adsorption β of P3HT and MEH-CN-PPV homopolymer. The spectra of respective homo polymers are different. NEXAFS spectra of the blend system with different blend ratio can be obtained by a linear superposition of the spectra of P3HT and MEH-CN-PPV homopolymer weighted with the corresponding blend ratio. The dispersion δ spectra of P3HT and MEH-CN-PPV reveal positive and negative values and differ strongly depending X-ray energy. Therefore, the scattering contrast depends on the X-ray energy. Using the adsorption β spectra, the penetration depth Λ of the soft X-rays into the blend film is calculated as shown in Figure 16b. Figure 17 indicates the GI-RSoXS patterns of as spun P3HT/MEH-CN-PPV bulk heterojunction films with a P3HT content of 70 wt% for different energies from 280 to 289 eV (wavelength of X-rays from 4.43 to 4.29 nm). Although the wavelength is varied by only 3%, the scattering patterns significantly change. For energy below 284 eV, an intensity oscillation in vertical direction is observed, which comes from the correlated roughness originating from the interference of scattered X-rays from different interfaces. The correlated roughness vanishes with increasing energies of the X-rays. It indicates no scattering signal from the substrate interface is detected and X-ray penetrates near the surface as shown in Figure 17. The low scattering intensity of GI-RSoXS at 284 eV (Figure 17c) is due to the very low incident intensity at this energy. The reduction of the intensity at 286 eV (Figure 17e) can be ascribed to the significant low contrast between P3HT and MEH-CN-PPV at this energy. Thus, the surface structure is accessible at 286 eV. The change in the total scattered intensity was attributed to the changed contrast conditions from the contrast variation.